Made available in DSpace on 2014-12-17T15:32:17Z (GMT). No. of bitstreams: 1 GustavoPG.pdf: 468493 bytes, checksum: 5f74059b167ef7c7965d0eddca9dca0c (MD5) Previous issue date: 2006-05-19 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The aim of this study was to evaluate the clinical and pathological features of cases diagnosed as dentigerous cyst by the Department of Oral Pathology, School of Dentristy at the Federal University of Rio grande do Norte, attempting the possible correlation between histomorphological findings and epidemiological data contained at the files of the patients, in order to define a suggested variation of lesion named inflammatory dentigerous cyst. It was verified that dentigerous cyst are more frequently present in the earfy three decades of life, with the majority of cases occuring in the second decade (40,740/0 ), and also male (57,41%) and white patients (68,52%) were most affected. In relation to anatomic site, the dentigerous cyst was more prevalent at anterior maxila and posterior mandible, showing, usually, a slow growth pattem. The majority of lesions were asymptomatic and the radiographic observed was frequently na unilocular radiolucency. In regard to the histomorphological analysis, it was noticed that the lesions showed commonly a thin epithelium, with a capsule of fibrous connective tissue, richly vascularized and collagenized with an intense mononuclear inflammatory infiltrate. Finally, clinicopathological was performed and it was find out that cysts that showed a thick epithelium, with a high degree of vascularization and collagenization, intense inflammatory infiltrate in the cystic capsule, were located in the pre-molares region, in patients under 12 years old and the majority showing painfull sintomatology, properly, compatible with inflammatory dentigerous cyst. The findings of the present study indicate that, probably, there is a variant of the dentigerous cyst, and therefore, we suggest the denomination inflammatory follicular cyst for this entity / Este estudo teve como objetivo realizar uma analise clinicopatologica de casos diagnosticados como cisto dentigeno na Disciplina de Patologia Oral do Departamento de Odontologia da UFRN,enfatizando uma possivel correla??o entre os achados histomorfologicos e os dados epidemiologicos constantes nas fichas de solicita??o de exame histopatologico,no intuito de caracterizar uma possivel variante da les?o,denominada cisto dentigero de origem inflamatoria.Foi verificado que o cisto dentigero apresentou-se com maior frequencia nas tres primeiras decadas de vida,com maior numero de casos observados na segunda decada(40,74%);tendo sido o genero masculino o mais afetado,com 57,41% dos casos,e os pacientes leucodermas os mais acometidos com 68,52% da amostra total.A regiao anatomica mais atingida foi a anterior de maxila e a posterior de mandibula, sendo o padrao de crescimento lento o mais comumente observado.A maioia das lesoes apresentou-se assintomatica,e o aspecto radiografico frequentemente identificado foi o radiolucido inilocular.Quanto a analise histomorfologica,observou-se que as lesoes

Made available in DSpace on 2014-12-17T15:32:21Z (GMT). No. of bitstreams: 1 PedroCRN_DISSERT.pdf: 3117630 bytes, checksum: 2544a3022fe67731388c2238d7dc5289 (MD5) Previous issue date: 2012-02-29 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / Odontogenic cysts are pathologic cavities covered by odontogenic epithelium and filled by liquid, desquamated cells or other materials. The intraosseous lesions, such as radicular cyst and dentigerous cyst, present a potential of expansion capable of promoting the destruction of the surrounding osseous tissue. The mechanisms related to this process of expansion are the proliferation of cystic epithelium, the increase of the osmolarity of the cystic fluid and the synthesis of reabsorption factors such as IFN-? and TGF-?1. The aim of this study was to evaluate and compare the immunohistochemical expression of IFN-? and TGF-?1 between radicular cysts and dentigerous cysts in order to understand the role and behavior of these proteins in the expansion of these cysts. We selected 20 cases of radicular cyst and 20 cases of dentigerous cyst chosen from the files of UFRN s Laboratory of Oral Pathology. After analyzing the clinical data, the cases underwent the routine staining technique (HE) and immunohistochemistry for the appearance of IFN-? and TGF-?1 in the epithelium and capsule of these cysts. The statistical analysis using the Mann-Whitney test revealed no statistically significant difference in immunoexpression of IFN-? between the epithelium (p = 0.565) and capsules (p = 0.414) of radicular cysts and dentigerous cysts. Moreover, there was no statistically significant difference of immunoexpression of TGF-?1 between the epithelium (p = 0.620) and capsules (p = 0.056) of radicular cysts and dentigerous cysts. The Wilcoxon test revealed no statistically significant difference between IFN-? and TGF-?1 imunoexpressions in the epithelium (p = 0.225) and capsules (p = 0.370) of radicular cysts. There was no statistically significant difference between IFN-? and TGF-?1 imunoexpressions in the epithelium (p = 0.361) of dentigerous cysts. However, there was a statistically significant difference between IFN-? and TGF-?1 immunoexpressions in the capsule (p = 0.001) of dentigerous cysts, being TGF-?1 the factor which presented the most significant immunoexpression. Given these results, we conclude that there was no difference in immunohistochemical expression of IFN-? and TGF-?1 between radicular and dentigerous cysts and that TGF-?1 was more significant than the IFN-? in the capsule of dentigerous cysts / Os cistos odontog?nicos s?o cavidades patol?gicas revestidas por epit?lio odontog?nico e preenchidas por l?quido, c?lulas descamadas, ou outros materiais. As les?es intra-?sseas, como o cisto radicular e o cisto dent?gero, apresentam um potencial de expans?o capaz de promover a destrui??o do tecido ?sseo circunjacente. Os mecanismos relacionados a esse processo de expans?o s?o a prolifera??o do epit?lio c?stico, o aumento da osmolaridade do fluido c?stico e a s?ntese de fatores de reabsor??o ?ssea como IFN-? e TGF-?1. O objetivo deste estudo foi avaliar e comparar a express?o imuno-histoqu?mica do IFN-? e do TGF-?1 entre cistos radiculares e cistos dent?geros com a finalidade de compreender o papel e o comportamento dessas prote?nas no processo de expans?o destes cistos. Selecionamos 20 casos de cisto radicular e 20 casos de cisto dent?gero retirados dos arquivos do Laborat?rio de Patologia Oral da UFRN. Ap?s an?lise dos dados cl?nicos, os casos foram submetidos a t?cnica de colora??o de rotina (HE) e ao m?todo imuno-histoqu?mico para evidencia??o da express?o do IFN-? e do TGF-?1 no epit?lio e na c?psula dos referidos cistos. A an?lise estat?stica dos dados utilizando o teste de Mann-Whitney revelou que n?o houve diferen?a estatisticamente significativa da imunoexpress?o do IFN- entre os epit?lios (p=0,565) e c?psulas (p=0,414) dos cistos radiculares e cistos dent?geros. Al?m disso, n?o houve diferen?a estatisticamente significativa da imunoexpress?o do TGF-?1 entre os epit?lios (p=0,620) e c?psulas (p=0,056) dos cistos radiculares e cistos dent?geros. O teste de Wilcoxon revelou que n?o houve diferen?a estatisticamente significativa entre as imunoexpress?es do IFN- e do TGF-?1 no epit?lio (p=0,225) e na c?psula (p=0,370) dos cistos radiculares. N?o houve diferen?a estatisticamente significativa entre as imunoexpress?es do IFN- e do TGF-?1 no epit?lio (p=0,361) dos cistos dent?geros. No entanto, houve diferen?a estatisticamente significativa entre as imunoexpress?es do IFN- e do TGF-?1 na c?psula (p=0,001) dos cistos dent?geros, sendo o TGF-?1 o que apresentou a imunoexpress?o mais significativa. Diante destes resultados, conclu?mos que n?o houve diferen?a de express?o imuno-histoqu?mica do IFN-? e do TGF-?1 entre os cistos radiculares e dent?geros e que o TGF-?1 foi mais expressivo do que o IFN-? na c?psula dos cistos dent?geros

Made available in DSpace on 2014-12-17T15:32:23Z (GMT). No. of bitstreams: 1 VivianeAOM_DISSERT.pdf: 3188421 bytes, checksum: 338aa2ec46045198a33e6eea1db0d483 (MD5) Previous issue date: 2014-02-13 / The radicular cysts (RCs) and dentigerous (DCs), despite having different etiologies, form a pathological cavity lined by epithelium, which grows due to the buildup of fluid inside, as the surrounding bone is reabsorbed and the epithelium will being induced to proliferate. The epithelial proliferation, which has been identified as one of the key processes in the growth of odontogenic cystic lesions, is influenced by growth factors such as EGFR (epidermal growth receptor factor) and podoplanin (PDPN), many of which may have its production stimulated mainly during inflammatory processes. The objective of this research was to evaluate and compare the immunohistochemical expression of EGFR and PDPN in 30 cases of RCs and 30 cases of DCs, semiquantitatively, in light microscopy, associating it with the degree of inflammation, cellular localization of immunostaining and with the immunostained epithelial layers. Data were statistically analyzed by Chi-square test and Fisher exact test, considering a significance level of 5 %. The results showed high immunoreactivity of both proteins in the lesions studied, only statistically significant difference was observed in immunostaining of PDPN (p=0.033), which proved higher in RCs. The other analyzed parameters showed no relevant significant differences. We conclude that, as EGFR and PDPN showed high immunoreactivity in cystic lesions analyzed, these proteins participate the pathogenesis of these lesions through the epithelial stimulation process, despite having different etiologies. Furthermore, it can infer that the higher immunostaining of PDNP in RCs that DCs showed no distinction indicator between the two lesions, regarding their etiologies, once this protein also showed a considerable expression in DCs, independent of the intensity of the inflammatory infiltrate / Os cistos radiculares (CRs) e dent?geros (CDs), apesar de possu?rem etiologias diferentes, formam uma cavidade patol?gica revestida por epit?lio, a qual cresce em fun??o do ac?mulo de l?quido em seu interior, ? medida que o osso ao redor ? reabsorvido e o epit?lio vai sendo induzido a proliferar. A prolifera??o epitelial, que tem sido apontada como um dos processos determinantes no crescimento das les?es c?sticas odontog?nicas, ? influenciada por fatores de crescimento como o EGFR (receptor do fator de crescimento epid?rmico) e a podoplanina (PDPN), muitos dos quais podem ter sua produ??o estimulada principalmente durante processos inflamat?rios. O objetivo desta pesquisa foi avaliar e comparar a express?o imunoistoqu?mica do EGFR e da PDPN em 30 casos de CRs e 30 casos de CDs, de forma semiquantitativa, em microscopia de luz, associando-a com o grau de inflama??o, localiza??o celular da imunocolora??o e com as camadas epiteliais imunomarcadas. Os dados foram avaliados estatisticamente por meio de testes do Qui-quadrado e Exato de Fisher, considerando-se um n?vel de signific?ncia de 5%. Os resultados mostraram que houve elevada imunorreatividade das duas prote?nas nas les?es estudadas, sendo observada apenas diferen?a estat?stica significativa na imunoexpress?o da PDPN (p=0,033), que se mostrou mais elevada nos CRs. Os demais par?metros analisados n?o demonstraram diferen?as significativas relevantes. Conclui-se que, como o EGFR e a PDPN apresentaram elevada imunoexpress?o nas les?es c?sticas analisadas, essas prote?nas participam da patog?nese dessas les?es atrav?s da estimula??o epitelial, apesar de apresentarem etiologias diferentes. Al?m disso, pode-se inferir que a maior imunomarca??o da PDPN em CRs do que em CDs n?o se mostrou indicador de distin??o entre as duas les?es, com rela??o ?s suas etiologias, uma vez que nestes ?ltimos essa prote?na tamb?m apresentou express?o consider?vel, independente da intensidade do infiltrado inflamat?rio