Thesis (Ph. D.)--University of Washington, 1995. / Vita. Includes bibliographical references (leaves [76]-83).

Diss. (sammanfattning) Stockholm : Karolinska institutet, 2007. / Härtill 6 uppsatser.

Thesis (Ph. D.)--University of Washington, 2005. / Vita. Includes bibliographical references (leaves 70-88).

Thesis (Ph. D.)--University of Washington, 2001. / Vita. Includes bibliographical references (leaves [109]-122).

Submitted by Franciele Moreira (francielemoreyra@gmail.com) on 2018-06-22T20:27:16Z No. of bitstreams: 2 Dissertação - Larissa Córdova Turones - 2018.pdf: 2576144 bytes, checksum: fb628d04d3221c6ac7ccaf79cc3d60ea (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Rejected by Jaqueline Silva (jtas29@gmail.com), reason: on 2018-06-22T20:38:02Z (GMT) / Submitted by Franciele Moreira (francielemoreyra@gmail.com) on 2018-06-22T20:39:29Z No. of bitstreams: 2 Dissertação - Larissa Córdova Turones - 2018.pdf: 2494325 bytes, checksum: 52754820438d7cd88e3dc298a96a898a (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Approved for entry into archive by Luciana Ferreira (lucgeral@gmail.com) on 2018-06-27T11:25:24Z (GMT) No. of bitstreams: 2 Dissertação - Larissa Córdova Turones - 2018.pdf: 2494325 bytes, checksum: 52754820438d7cd88e3dc298a96a898a (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) / Made available in DSpace on 2018-06-27T11:25:24Z (GMT). No. of bitstreams: 2 Dissertação - Larissa Córdova Turones - 2018.pdf: 2494325 bytes, checksum: 52754820438d7cd88e3dc298a96a898a (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2018-05-16 / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPES / The Bj-PRO-7a, a heptapeptide isolated and identified from Bothrops jararaca (Bj) venom, evoke potent therapeutic effects, as antihypertensive effect, natriuretic and diuretic effects, promotion of angiogenisis and vasodilatation. The effects of heptapeptide are independent of Angiotensin Converting Inhibitor (ACE), possibly dependent of Muscarinic Receptors subtype 1 (M1R) activation and oxido nitric pathways. Also, the Bj-PRO-7a actions upon central nervous system still need to be evaluated. Thus, the aims of this study were: i) to assess the effects of acute administration of Bj-PRO-7a upon behavior; ii) to reveal mechanisms involved in the effects of Bj- PRO-7a upon locomotion/exploration, anxiety and depression-like behaviors. For this purpose, adult male Wistar (WT) and Spontaneous Hypertensive Rats (SHRs) (300-380 g) received i.p. injections of Vehicle (NaCl 0.9%), Diazepam (2 mg/kg), Imipramine (15 mg/kg), Bj-PRO-7a (71, 213 or 426 nmol/kg), Pirenzepine (852 nmol/kg), α-metil-DL-tirosina (200 mg/kg) or Chlorpromazine (2 mg/kg) and were placed in the Elevated Plus Maze (EPM), Open Field (OF) and Forced Swimming (FS) tests. The heptapeptide promoted anxiolytic and antidepressantlike effects and increased the locomotion/exploration. These effects of Bj-PRO-7a seem to be strongly dependent on activation of M1R, catecholaminergic paths and dopaminergic receptors. / O Bj-PRO-7a, um heptapeptídeo isolado e identificado no veneno da Bothrops jararaca (Bj), evoca potentes efeitos terapêuticos, tais como o efeito anti-hipertensivo, efeitos natriurético e diurético, promoção da angiogênese e vasodilatação. Os efeitos do hepapeptídeo são independentes da inibição sobre a Enzima Conversora de Angiotensina (ECA), possivelmente dependentes da ativação dos Receptores Muscarínicos de Acetilcolina subtipo 1 (M1Rs) e vias do óxido nítrico. Ademais, as ações do Bj-PRO-7a sobre o sistema nervoso central ainda precisam ser avaliadas. Assim, os objetivos deste estudo foram: i) acessar os efeitos da administração aguda do Bj-PRO-7a sobre o comportamento; ii) revelar os mecanismos envolvidos nos efeitos do Bj-PRO-7a sobre a locomoção/exploração, comportamento tipo-ansiedade e depressão. Para esse propósito, ratos machos adultos Wistar (WT) e Espontaneamente Hipertensos (SHR) (300-380 g) receberam injeções i.p. de Veículo (NaCl 0,9%), Diazepam (2 mg/kg), Imipramina (15 mg/kg), Bj-PRO-7a (71, 213 ou 426 nmol/kg), Pirenzepina (852 nmol/kg), α-metil-DL-tirosina (200 mg/kg) ou Clorpromazina (2 mg/kg) e foram colocados nos testes de Labirinto em Cruz Elevado (LCE); Campo Aberto (CA) e Nado Forçado (NF). O heptapeptídeo promoveu efeito tipo-ansiolítico e antidepressivo e aumentou a locomoção/exploração. Esses efeitos parecem ser fortemente dependentes da ativação dos M1Rs, vias catecolaminérgicas e receptores dopaminérgicos.