The purpose of this study was to rationally design animal component free, chemically defined serum free media (ACF-CD-SFM) for MRC-5 and Vero cells while adhering to the Quality by Design guidelines. This was achieved by using the Modified Vero Serum Free Medium (MVSFM) as the basal formulation and supplementing it with various combinations of growth factors (LONG® EGF, LONG® R3 IGF-I, rTransferrin, bFGF, TGF-3 and PDGF-AA), lipids (linoleic acid, cholesterol, and dexamethasone), lipid precursors (ethanolamine and phosphoethanolamine) and vitamins (all-trans retinoic acid, -tocopherol and ascorbic acid). Media development was achieved by conducting a series of steps using different experimental methodologies with the end goal of satisfying the requirements of each cell line. MRC-5 and Vero cells were each cultured in specific media containing unique concentrations of supplements that were prepared according to the different statistical design methodologies. The original objective was to create a SFM, however due to the stringent nutritious requirements of anchorage dependent cell lines, only a reduction to 0.5% FBS was achieved. For MRC-5 cells, the one-factor-at-a-time (OFAT) generated the Prototype + 0.5% FBS medium. The Definitive Screening Design (DSD) gave rise to the Delta 1 + 0.5% FBS, which was the optimum medium formulation for MRC-5 cells as it had comparable cell yields to DMEM + 10 % FBS. This result was confirmed by the Genetic Algorithms-Hill Climbing (GA-HC) method. In the case of Vero cells, the OFAT and the DSD confirmed that MVSFM + 0.5 % FBS was the most optimal formulation. The morphology in both media for both cell lines was comparable to that in DMEM-10% FBS. It was concluded that the DSD method successfully achieved a reduction of the serum concentration from 10% to 0.5% FBS. / October 2017

Thesis (M.F.A.)--Rochester Institute of Technology, 2009. / Typescript. Includes bibliographical references (p. 139-141).

Thesis (Master)--İzmir Institute of Technology, İzmir, 2006. / Keywords: Globalization, local culture. Includes bibliographical references (leaves.74-79).

This study examines the ways in which the concept of &#039 / national design&#039 / informs evaluation of products and their designers, through the example of the field of industrial design in Turkey and the recently influential design magazine Art+Decor. For this purpose, first of all, &#039 / evaluation&#039 / is analyzed as a means in which meanings are imposed on products, and as a tool in struggles for positions and status within the field of industrial design. Then, the role of &#039 / nationality&#039 / in such a function of evaluation is investigated. Finally, a case study is provided, in which the employment of the concept of &#039 / Turkish design&#039 / in evaluation of products and designers is analyzed within the texts published in Art+Decor magazine between 2003 and 2005.

Este estudo trata de exposições de fins educativos que tenham como objetivo oferecer uma reflexão sobre design. Para tanto, importa conceber a exposição como modalidade de projeto, o que torna oportuna a fundamentação teórica em design de exposição, alternativa na área do design gráfico-ambiental. As bases conceituais do trabalho compreendem a exposição como produto que constitui variável na percepção dos visitantes. O objetivo geral é elaborar critérios projetuais a partir da análise, em três estudos, sobre as ações de organizadores e designers, com vistas à comunicação com o público. A pesquisa é de caráter exploratório, para a qual lançamos mão de entrevistas semi-estruturadas. Os casos analisados, em três cidades do país, nos anos de 2010 e 2011, revelaram que, além de contribuir para a difusão do design, estes eventos constituem modos de refletir sobre os desafios do design e o papel do designer na contemporaneidade. / This study concerns educative exhibitions that aim to offer a reflection on design. Therefore the exhibition is conceived as a design type of project, which makes timely the theoretical foundations on exhibition design, an alternative in environmental-graphic design. The conceptual basis comprehends the exhibition as a product which is a variable in the visitors’ perception. The aim is to generate project criteria throughout the analysis in three studies regarding the actions of organizers and designers, considering the communication with the audience. The research is of exploratory kind, to which we lay hold of semi-structured interviews. The cases analyzed in three Brazilian cities during the years of 2010 and 2011 revealed that, besides its contribution to the diffusion of design, these events consist ways of reflecting about the challenges of design and the designer's role in contemporary society.

Este estudo trata de exposições de fins educativos que tenham como objetivo oferecer uma reflexão sobre design. Para tanto, importa conceber a exposição como modalidade de projeto, o que torna oportuna a fundamentação teórica em design de exposição, alternativa na área do design gráfico-ambiental. As bases conceituais do trabalho compreendem a exposição como produto que constitui variável na percepção dos visitantes. O objetivo geral é elaborar critérios projetuais a partir da análise, em três estudos, sobre as ações de organizadores e designers, com vistas à comunicação com o público. A pesquisa é de caráter exploratório, para a qual lançamos mão de entrevistas semi-estruturadas. Os casos analisados, em três cidades do país, nos anos de 2010 e 2011, revelaram que, além de contribuir para a difusão do design, estes eventos constituem modos de refletir sobre os desafios do design e o papel do designer na contemporaneidade. / This study concerns educative exhibitions that aim to offer a reflection on design. Therefore the exhibition is conceived as a design type of project, which makes timely the theoretical foundations on exhibition design, an alternative in environmental-graphic design. The conceptual basis comprehends the exhibition as a product which is a variable in the visitors’ perception. The aim is to generate project criteria throughout the analysis in three studies regarding the actions of organizers and designers, considering the communication with the audience. The research is of exploratory kind, to which we lay hold of semi-structured interviews. The cases analyzed in three Brazilian cities during the years of 2010 and 2011 revealed that, besides its contribution to the diffusion of design, these events consist ways of reflecting about the challenges of design and the designer's role in contemporary society.

Este estudo trata de exposições de fins educativos que tenham como objetivo oferecer uma reflexão sobre design. Para tanto, importa conceber a exposição como modalidade de projeto, o que torna oportuna a fundamentação teórica em design de exposição, alternativa na área do design gráfico-ambiental. As bases conceituais do trabalho compreendem a exposição como produto que constitui variável na percepção dos visitantes. O objetivo geral é elaborar critérios projetuais a partir da análise, em três estudos, sobre as ações de organizadores e designers, com vistas à comunicação com o público. A pesquisa é de caráter exploratório, para a qual lançamos mão de entrevistas semi-estruturadas. Os casos analisados, em três cidades do país, nos anos de 2010 e 2011, revelaram que, além de contribuir para a difusão do design, estes eventos constituem modos de refletir sobre os desafios do design e o papel do designer na contemporaneidade. / This study concerns educative exhibitions that aim to offer a reflection on design. Therefore the exhibition is conceived as a design type of project, which makes timely the theoretical foundations on exhibition design, an alternative in environmental-graphic design. The conceptual basis comprehends the exhibition as a product which is a variable in the visitors’ perception. The aim is to generate project criteria throughout the analysis in three studies regarding the actions of organizers and designers, considering the communication with the audience. The research is of exploratory kind, to which we lay hold of semi-structured interviews. The cases analyzed in three Brazilian cities during the years of 2010 and 2011 revealed that, besides its contribution to the diffusion of design, these events consist ways of reflecting about the challenges of design and the designer's role in contemporary society.

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No description available.

Com base em um referencial teórico que incluiu a história da arte e do design, a semiótica e estu-dos da cor na cultura, esta tese defende a ideia de cor como linguagem e construção cultural, observando seu uso em diferentes sistemas de significação, no design e, especialmente, no design de embalagens. O trabalho parte da evolução da linguagem visual da embalagem, observada en-tre o final do século XIX e o início do século XXI, expondo as mudanças ocorridas no design como expressão dos diferentes momentos da sociedade e da cultura visual ocidental, e resgatando a importância histórica das cores para a comunicação. Num segundo momento revisam-se funda-mentos da semiótica, transpondo-os para a abordagem da cor como signo e para a investigação da linguagem cromática como um sistema. Para explicitar os princípios estruturais dessa lingua-gem, os significados atribuídos às cores e aos atributos cromáticos são revisados por meio de pesquisa bibliográfica. Através de estudo empírico, esta tese examina a linguagem cromática do design de embalagens contemporâneo, a partir de um corpus de embalagens de alimentos co-mercializados no Brasil. Os resultados mostraram que o sistema de significados da cor no design de embalagens se apoia tanto nos campos associativos gerados pelos atributos de matiz, clarida-de e saturação das cores, quanto nas oposições cromáticas e semânticas que tais características criam. A abordagem do estudo foi além da análise estrutural, envolvendo simultaneamente aspec-tos sintáticos, semânticos e pragmáticos da cor, buscando explicitar sua utilização como instru-mento para a difusão de ideias e valores. Foi observado que as cores assumem funções visuais, icônicas, indiciais e simbólicas na comunicação da embalagem. No nível simbólico, os sentidos mais frequentes assumidos pelas cores corresponderam a diferentes discursos que orientam as práticas alimentares contemporâneas. A pesquisa mostrou que o design dialoga com outros sis-temas de significação e resgata, difunde e reelabora o simbolismo das cores para construir ima-gens de qualidade, pureza, bem-estar, saúde, ecologia e feminilidade, entre outras, refletindo concepções ideológicas e culturais que têm lugar na vida contemporânea. / Based on a theoretical referential which included art and design history, semiotics and cultural studies of color, this thesis supports the idea of color as language and cultural construction, observing its use in different systems of signification, in design and, particularly, in packaging design. The work starts from the evolution of visual language of packaging, observed between late 19th century and early 21st century, showing the changes occurred in design as an expression of the different moments of western society and visual culture, and bringing back the historical importance of colors in communication. Secondly, the work reviews the fundamentals of semiotics, transposing them to the approach of the color as a sign, and to the investigation of color language as a system. In order to explain the structural principles of this language, the meanings of colors and chromatic attributes are reviewed through bibliographical research. Through an empirical study, this thesis examines the chromatic language of contemporary packaging design, from a corpus of food packaging commercialized in Brazil. The results showed that the system of significations of color in packaging design leans both on the associative fields generated by the attributes of color -- hue, brightness and saturation -- and on the chromatic and semantic oppositions that such characteristics create. The study approach went beyond the structural analysis, involving simultaneously syntactic, semantic and pragmatic aspects of color, trying to explain its use as a tool for the diffusion of ideas and values. It was observed that the colors assume visual, iconic, indexical and symbolic functions in the packaging communication. In the symbolic level, the most frequent senses assumed by the colors corresponded to different discourses which guide the contemporary food practices. The research showed that design communicates with other systems of signification and rescues, spreads out and reconstructs the color symbolism to construct images of quality, pureness, welfare, health, ecology and femininity, among others, reflecting ideological and cultural conceptions which have their place in contemporary life.