Submitted by (edna.saturno@ufrpe.br) on 2016-10-06T12:22:38Z No. of bitstreams: 1 Elizabeth Sampaio de Medeiros.pdf: 1026668 bytes, checksum: c3f8468fa38781ce0ffddc9e51ab6c38 (MD5) / Made available in DSpace on 2016-10-06T12:22:38Z (GMT). No. of bitstreams: 1 Elizabeth Sampaio de Medeiros.pdf: 1026668 bytes, checksum: c3f8468fa38781ce0ffddc9e51ab6c38 (MD5) Previous issue date: 2008-02-25 / The goal of this research was to evaluate the occurrence and the sensitivity of Staphylococcus spp to antibiotics and disinfectants used in the control of bovine mastitis. Fifteen dairy farms located in the Metropolitan Recife regions (A), Agreste (B) and Zona de Mata (C), of the state of Pernambuco were studied. A total of 1,080 milk samples were collected and submitted to microbiological culture in order to isolate and identify Staphylococcus spp. To study the sensitivity and resistance of Staphylococcus spp to antimicrobials the diffusion disk technique with the following antibiotics was used: amoxiciline (10 mcg), ampiciline (10 mcg), azitromicine (15 mcg), cefquinome (30 mcg), cephalonium (30 mcg), ciprofloxacine (5mcg), cloxacillin (25 mcg), danofloxacine (10 mcg), enrofloxacine (5 mcg), eritromicine (15 mcg),florfenicol (30mcg), gentamicine (10 mcg), penicillin + novobiocine (10 mcg), sulfa (25 mcg) + trimetoprim (5 mcg),tobramicine (10 mcg) e tetraciclina (30 mcg) + neomicine (30 mcg) + bacitracine (10 mcg). For the in vitro study of sensitivity and resistance of S. aureus and Staphylococcus coagulase positive to the disinfectants, the following products were used: iodine (0.5%), chlorine (2.5%), quaternary ammonium (4%), lactic acid and chlorhexidine (2.0%). Of the 1,080 samples analyzed, 740 (68.5%) were positive and 340 (31.5%) negative. From the positive samples, Staphylococcus were isolated in 291 (39.3%). Of these, 170 (58.4%) were classified as coagulase negative Staphylococcus (SCN), 84(28.9%) as Staphylococcus aureus (S. aureus), and 37(12.7%) as coagulase positive Staphylococcus (SCP). The most efficient antibiotic in vitro was the combination between neomicine (98.4%), and bacitracine (99.3%) and tetracycline (89.7%) for regions A, B e C, respectively. The least efficient was ampicillin, which only showed a resistance of 56.5% to the isolates from region A, 72.8% for region B e 71.8% for region C. With respect to the disinfectants, iodine proved to be the most effective for S. aureus and SCP. The least effective was chlorine. Based on the results, it can be concluded with the epidemiological importance of Staphylococcus spp in subclinical mastitis in dairy cows in the regions chosen and the need for periodic evaluation of the antibiotics and disinfectants used to control mastitis. / Objetivou-se com esse trabalho avaliar a ocorrência e a sensibilidade de bactérias do gênero Staphylococcus spp frente aos antibióticos e desinfetantes utilizados no controle das mastites bovinas. Foram estudadas 15 propriedades situadas em municípios da Região Metropolitana do Recife (A), Agreste (B) e Zona da Mata (C) do estado de Pernambuco. Foram coletadas 1080 amostras de leite de vacas com mastite subclínica que foram submetidas ao exame microbiológico para isolamento e identificação dos Staphylococcus spp. Para o estudo da sensibilidade e resistência dos Staphylococcus spp aos antimicrobianos foi empregada a técnica de difusão em discos frente aos seguintes antibióticos: amoxicilina (10 mcg), ampicilina (10 mcg), azitromicina (15 mcg), cefquinome (30 mcg), cephalonium (30 mcg), ciprofloxacina (5mcg), cloxacilina (25 mcg), danofloxacina (10 mcg), enrofloxacina (5 mcg), eritromicina (15 mcg), florfenicol (30mcg), gentamicina (10 mcg), penicilina + novobiocina (10 mcg), sulfa (25 mcg) + trimetoprim (5 mcg), tobramicina (10 mcg) e tetraciclina (30 mcg) + neomicina (30 mcg) + bacitracina (10 mcg). Para o estudo “in vitro” da sensibilidade e resistência dos Staphylococcus aureus e Staphylococcus coagulase positiva aos desinfetantes, foram utilizados o iodo (0,57%), cloro (2,5%), amônia quaternária (4%), ácido lático e clorexidine (2,0%). Das 1080 amostras analisadas, 740 (68,5%) foram positivas ao exame microbiológico e 340 (31,5%) negativas. Das amostras positivas, isolaram-se bactérias do gênero Staphylococcus em 291 (39,3%). Dos 291 isolados, 170(58,4%) foram classificados como Staphylococcus coagulase negativa (SCN), 84(28,9%) como Staphylococcus aureus (S.aureus) e 37(12,7%) como Staphylococcus coagulase positiva (SCP). A melhor eficácia in vitro foi obtida pela associação entre neomicina + bacitracina + tetraciclina com percentuais de 98,4%, 99,3%, 89,7% para as regiões A, B e C respectivamente. O menos eficaz foi a ampicilina que apresentou 56,5% de resistência para os isolados da região A, 72,8% para a região B e 71,8% na região C. Com relação aos desinfetantes, observou-se que o iodo foi o mais eficaz para os S. aureus e SCP e o menos eficaz foi o cloro. Conclui-se com este estudo a importância epidemiológica dos Staphylococcus spp nas mastites subclínicas em vacas leiteiras nas regiões estudadas e a necessidade da avaliação periódica dos antibióticos e desinfetantes utilizados no controle da mastite.

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Quaternary ammonium compounds (QACs) have long served as lead disinfectants in residential, industrial, and hospital settings. Their simple yet effective amphiphilic nature makes them an ideal class of compounds through which to explore antibacterial activity. We have developed novel multiQAC scaffolds through simple and cost-efficient syntheses, yielding hundreds of diverse compounds strategically designed to examine various aspects of antibacterial and anti-biofilm activity, as well as toxicity. Many of these bis-, tris-, and tetraQACs display antibacterial activity 10 to 100 times greater than conventional monoQACs, and are among the most potent biofilm eradicators to date. Through analyzing their activity against several strains, we have uncovered and provided further evidence for key tenets of amphiphilic QAC bioactivity: a balance of hydrophobic side chains with cationic head groups generates optimal antibacterial activity, though toxicity to eukaryotic cells needs to be mitigated. Given their ubiquitous nature and chemical robustness, the overuse of QACs has led to the development of QAC resistance genes that are spreading throughout the microbial world at an alarming rate. These resistant strains, when found in bacterial biofilms, are able to persist in the presence of lead commercial QAC disinfectants, warranting the development of next-generation biocides. Several of our scaffolds were designed with QAC resistance machinery in mind; thus, we utilized these compounds not only as antibacterial agents but also as chemical probes to better understand and characterize QAC-resistance in methicillin-resistant Staphylococcus aureus (MRSA). Our findings support previous postulations that triscationic QACs would retain potency against QAC-resistant strains. Furthermore, we have identified monocationic and aromatic moieties, as well as conformational rigidity, as being more prone to recognition by the resistance machinery. Using our chemical toolbox comprised of QACs of various charge state and scaffold, we explored both the mechanism and scope of QAC-resistance by examining their structure-resistance relationship. Our holistic findings have allowed us to better understand the dynamics of this system towards the design and development of next-generation QACs that will: (1) allow us to better probe the resistance machinery, and (2) remain efficacious against a variety of microbial pathogens. / Chemistry

Orientador: Janaina Habib Jorge / Resumo: A desinfecção química associada ao método mecânico tem sido recomendada para a higienização de próteses removíveis parciais ou totais. Levando-se em consideração as desvantagens dos agentes químicos de limpeza utilizados para a desinfecção ou redução do biofilme das próteses, como o manchamento, branqueamento e corrosão das partes metálicas, novos estudos são necessários. Assim, o objetivo deste estudo foi avaliar as propriedades biológicas, físicas e mecânicas de uma resina acrílica para base de próteses após imersão em sabonetes líquidos desinfetantes, nas suas concentrações inibitórias minímas (CIM) para Candida albicans, após diferentes períodos de tempo. Primeiramente, a CIM de cada sabonete foi determinada. Amostras de resina acrílica (Vipi Wave®) foram confeccionadas e divididas em grupos para avaliação da capacidade de formação de biofilme (n=6), citotoxicidade (n=9), rugosidade (n=15), dureza (n=15) e alteração de cor (n=15), após imersão por 0, 7, 14, 21 e 28 dias, nas seguintes soluções: AD: imersão em água destilada a 37°C (grupo controle); SD: ciclos de imersão diária em sabonete Dettol®® a 0,39%, por 8 horas a temperatura ambiente, seguido de imersão em água destilada por 16 horas a 37°C, simulando a desinfecção noturna das próteses; SP: ciclos de imersão diária em sabonete Protex® a 3,12%, conforme descrito para o grupo anterior. SL: ciclos de imersão diária em sabonete Lifebuoy® a 0,78%, conforme descrito para o grupo SD. Além disso, a redução do biofilme de C... (Resumo completo, clicar acesso eletrônico abaixo) / Chemical disinfection associated with the mechanical method has been recommended for the cleaning of partial or full dentures. Taking into consideration the drawbacks of the cleaning chemicals used for disinfection or reduction of biofilm of prostheses, such as staining, bleaching and corrosion of metal parts, further studies are needed. The objective of this study was to evaluate the biological, physical and mechanical properties of an acrylic resin for denture base (Vipi Wave®) after immersion in liquid disinfectant soaps in their minimum inhibitory concentrations (MIC) for Candida albicans, after different periods of time. Samples of acrylic resin (Vipi Wave®) were made. Samples from acrylic resin (Vipi Wave®) were made and shared in groups for the assessment of the biofilm formation capacity (n=6), cytotoxicity (n=9), roughness (n=15), hardness (n=15) and color change (n=15) after immersion in the following solutions for 0, 7, 14, 21 and 28 days: AD: immersion in distilled water at 37 ° C (control group); SD: daily immersion cycles in soap Dettol® 0.39% for 8 hours at room temperature, followed by soaking in distilled water for 16 hours at 37 ° C, simulating the night disinfection of prostheses; SP: daily immersion cycles in soap Protex® to 3.12%, as described for the previous group. SL: daily immersion cycles in Lifebuoy® in soap to 0.78%, as described for the SD group. In soap Dettol® was found the MIC of 0.39% of the soap concentration; in Protex® 3.12% and in Lifebuoy® the MIC was 0.78%. In addition, capacity of reduction of the biofilm of Candida albicans formed on the surface of samples (n = 9) immersed for 8 hours (overnight) in the solutions was also evaluated. To know the biofilm-forming capacity, there were the forming unit count tests of colonies and alamarBlue® ...(Complete abstract electronic access below) / Mestre

This project focuses on two important topics which may pose hazards to human health. Firstly, drinking water disinfection by-products (DBPs), which are generated by the chemical disinfection of water have been investigated. What has not been shown is the effect of DBPs in human germ cells as well as somatic cells and whether oxidative stress is involved in the mechanism of genotoxic action. Three different DBPs (halo acetic acids: HAAs), together with the antioxidants – catalase and butylated hydroxyanisole (BHA), were investigated in peripheral blood cells and sperm from healthy individuals using the Comet assay and lymphocytes only using the micronucleus assay. Secondly, nanoparticles of the non-steroidal anti-inflammatory drugs (NSAIDs), aspirin and ibuprofen, have been investigated in patients with respiratory diseases, in the micronucleus assay and the Comet repair assay. NSAIDs inhibit cyclooxygenase enzyme activity, which plays part in tumour progression. In the Comet assay, BHA and catalase were able to reduce DNA damage in both cell types compared to HAAs alone. Similarly, in the micronucleus assay, micronuclei were reduced with the antioxidants, suggesting oxygen radical involvement in both assays. With the NSAIDs, reductions were seen for DNA damage in the micronucleus assay with aspirin and ibuprofen nanoparticles compared to their bulk forms. Using the Comet repair assay, aspirin and ibuprofen nanoparticles aided repair of DNA to a greater extent than their bulk counterparts, which in turn showed better repair compared to samples repaired without NSAIDs. These observations show the importance of DBPs and NSAIDs in genotoxic public health issues.

Disinfection of the municipal water systems is mostly achieved by means of chlorine addition at water treatment plants known as sources. Thus, there should be an adequate chlorine concentration at the source for an effective disinfection throughout the system by considering upper and lower limits of disinfectant. However, since the disinfectants are reactive and decays through the system, chlorine added at the source may not be enough to maintain desired disinfectant residuals which may lead to water quality problems in the water distribution system. Moreover, the disinfectants such as chlorine has also an effect to be carcinogen due to formation of disinfectant by-products. Thus, the system should balance the amount of disinfectant supplied while minimizing the health risk. In such a case, it is recommended that one or more booster disinfection stations can be located throughout the system. Such a method can provide more uniform distribution of the chlorine concentration while reducing the amount of the disinfectant used. In this thesis, optimum scheduling, and injection rates of the booster disinfection stations have been searched. The objective is to minimize the injected mass dosage rate subjected to the provision of adequate and more uniform residual concentration in the network. Determination of variable network hydraulics and chlorine concentrations is held out by EPANET network simulation sofware. A C++ code was developed to interface with EPANET by means of the EPANET Programmer&#039 / s Toolkit for linear optimization of the disinfectant mass dosage rate applied to the network.

Relatos sobre resistência de microrganismos a inúmeros produtos químicos-sintéticos convencionais, bem como demanda por tecnologias adequadas ao sistema de produção orgânico/agroecológico motivaram o desenvolvimento dessa pesquisa buscando soluções antimicrobianas originadas de extrações de vegetais. O objetivo deste trabalho foi avaliar atividade desinfetante em extrações de Jacaranda micrantha Cham. (caroba), selecionada baseado em investigação etnográfica sobre plantas medicinais nativas no sul do Brasil. Foram confrontadas as cepas padrões Salmonella Choleraesuis ATCC 10708 e Staphylococcus aureus ATCC 25923, bem como as isoladas em alimentos de origem animal Salmonella spp (N=20) e Staphylococcus coagulase positiva (N=20). O método foi o de diluição, pelo teste de suspensão quantitativo para avaliar atividade bactericida de desinfetantes e antissépticos químicos. As formas foram decocto e extrato hidroetanólico (EH). A triagem, com os organismos padrões, ocorreu nas proporções (p : v) 5 g : 100 mL, 10 g : 100 mL e 20 g : 100 mL, hidratados ao volume inicial. As densidades populacionais (DP) de confronto foram 107, 106 e 105 UFCmL-1, nos tempo de contato de oito e 24 horas. A proporção 10 g : 100 mL foi usada para confrontar as isoladas. Verificada a identidade fitoquímica detectou-se a presença de compostos fenólicos, taninos condensados e flavonas tanto no decocto quanto maceração hidroacoólica, e de saponinas apenas no decocto. A triagem evidenciou que o decocto praticamente não promoveu redução das densidades populacionais frente as duas padrões, o mesmo ocorrendo frente às isoladas. Já o EH, na menor proporção p : v, inativou as cepas padrões na menor DP, e nas proporções 10 e 20 : 100 todas DP (exceção de 107 da Salmonella, apenas com redução). Frente as isoladas de Salmonella spp., inativou 5 % delas nas DP 106 e 105 UFCmL-1, e frente as Staphylococcus coagulase positiva, 5 % na DP 107, 40 % na 106 e 75 % na 105 UFCmL-1. Concluiu-se que o decocto pouco apresentou atividade antibacteriana, enquanto que o EH promoveu ação de redução logarítmica das densidades populacionais ou mesmo de inativação total frente todas as bactérias. As evidências mostram que além da densidade populacional de confronto, características intrínsecas dos indivíduos isolados dos dois gêneros também interferiram no tempo necessário para que a ação fosse promovida. / Reports of resistance of microorganisms to many conventional chemical-synthetic products, as well as the demand for appropriate organic / agroecological system technologies motivated the development of this research originated seeking antimicrobial solutions for extractions vegetables. The objective of this study was to assess disinfectant activity extractions Jacaranda micrantha Cham. (caroba), selected based on ethnographic research on native medicinal plants in southern Brazil. Standards strains Salmonella Choleraesuis ATCC 10708 and Staphylococcus aureus ATCC 25923 were compared, as well as isolates in foods of animal origin: Salmonella spp (N = 20) and Staphylococcus coagulase positive (N = 20). The method was to dilution by the quantitative suspension test for evaluating bactericidal activity of chemical disinfectants and antiseptics. The forms were hydroethanolic extract (HE) and decoction, screening with standards strains, occurred in the proportions (p: v) 5 g: 100 mL, 10 g: 100 ml and 20 g: 100 ml, hydrated to the original volume. Population densities (PD) of confrontation were 107, 106 and 105 UFCmL-1, the contact time of 8 and 24 hours. The ratio 10 g: 100 mL was used to confront the isolates. Verified the identity phytochemical detected the presence of phenolic compounds, flavones and condensed tannins as hidroacolic both the decoction and saponins only decoction. The screening showed that the decoction hardly promoted reduction of population densities across the two standards, the same occurring against isolated. Have the HE, the lowest proportion p: v, inactivated strains patterns in lower PD, and in the proportions 10 and 20: 100 all PD (except for the 107 Salmonella, only reduction). Against isolates of Salmonella spp. 5% of inactivated at 106 and 105 UFCmL-1 PD and against Staphylococcus coagulase positive in 5% PD 107, 40% 106 and 75% at 105 UFCmL-1. It was concluded that the decoction showed little antibacterial activity, while the hydroalcoholic extract promoted action log reduction in population density or total inactivation forward all bacteria. Evidence shows that in addition to population density of confrontation, intrinsic characteristics of isolated individuals of both genders also interfered in the time required for the action to be promoted.

Relatos sobre resistência de microrganismos a inúmeros produtos químicos-sintéticos convencionais, bem como demanda por tecnologias adequadas ao sistema de produção orgânico/agroecológico motivaram o desenvolvimento dessa pesquisa buscando soluções antimicrobianas originadas de extrações de vegetais. O objetivo deste trabalho foi avaliar atividade desinfetante em extrações de Jacaranda micrantha Cham. (caroba), selecionada baseado em investigação etnográfica sobre plantas medicinais nativas no sul do Brasil. Foram confrontadas as cepas padrões Salmonella Choleraesuis ATCC 10708 e Staphylococcus aureus ATCC 25923, bem como as isoladas em alimentos de origem animal Salmonella spp (N=20) e Staphylococcus coagulase positiva (N=20). O método foi o de diluição, pelo teste de suspensão quantitativo para avaliar atividade bactericida de desinfetantes e antissépticos químicos. As formas foram decocto e extrato hidroetanólico (EH). A triagem, com os organismos padrões, ocorreu nas proporções (p : v) 5 g : 100 mL, 10 g : 100 mL e 20 g : 100 mL, hidratados ao volume inicial. As densidades populacionais (DP) de confronto foram 107, 106 e 105 UFCmL-1, nos tempo de contato de oito e 24 horas. A proporção 10 g : 100 mL foi usada para confrontar as isoladas. Verificada a identidade fitoquímica detectou-se a presença de compostos fenólicos, taninos condensados e flavonas tanto no decocto quanto maceração hidroacoólica, e de saponinas apenas no decocto. A triagem evidenciou que o decocto praticamente não promoveu redução das densidades populacionais frente as duas padrões, o mesmo ocorrendo frente às isoladas. Já o EH, na menor proporção p : v, inativou as cepas padrões na menor DP, e nas proporções 10 e 20 : 100 todas DP (exceção de 107 da Salmonella, apenas com redução). Frente as isoladas de Salmonella spp., inativou 5 % delas nas DP 106 e 105 UFCmL-1, e frente as Staphylococcus coagulase positiva, 5 % na DP 107, 40 % na 106 e 75 % na 105 UFCmL-1. Concluiu-se que o decocto pouco apresentou atividade antibacteriana, enquanto que o EH promoveu ação de redução logarítmica das densidades populacionais ou mesmo de inativação total frente todas as bactérias. As evidências mostram que além da densidade populacional de confronto, características intrínsecas dos indivíduos isolados dos dois gêneros também interferiram no tempo necessário para que a ação fosse promovida. / Reports of resistance of microorganisms to many conventional chemical-synthetic products, as well as the demand for appropriate organic / agroecological system technologies motivated the development of this research originated seeking antimicrobial solutions for extractions vegetables. The objective of this study was to assess disinfectant activity extractions Jacaranda micrantha Cham. (caroba), selected based on ethnographic research on native medicinal plants in southern Brazil. Standards strains Salmonella Choleraesuis ATCC 10708 and Staphylococcus aureus ATCC 25923 were compared, as well as isolates in foods of animal origin: Salmonella spp (N = 20) and Staphylococcus coagulase positive (N = 20). The method was to dilution by the quantitative suspension test for evaluating bactericidal activity of chemical disinfectants and antiseptics. The forms were hydroethanolic extract (HE) and decoction, screening with standards strains, occurred in the proportions (p: v) 5 g: 100 mL, 10 g: 100 ml and 20 g: 100 ml, hydrated to the original volume. Population densities (PD) of confrontation were 107, 106 and 105 UFCmL-1, the contact time of 8 and 24 hours. The ratio 10 g: 100 mL was used to confront the isolates. Verified the identity phytochemical detected the presence of phenolic compounds, flavones and condensed tannins as hidroacolic both the decoction and saponins only decoction. The screening showed that the decoction hardly promoted reduction of population densities across the two standards, the same occurring against isolated. Have the HE, the lowest proportion p: v, inactivated strains patterns in lower PD, and in the proportions 10 and 20: 100 all PD (except for the 107 Salmonella, only reduction). Against isolates of Salmonella spp. 5% of inactivated at 106 and 105 UFCmL-1 PD and against Staphylococcus coagulase positive in 5% PD 107, 40% 106 and 75% at 105 UFCmL-1. It was concluded that the decoction showed little antibacterial activity, while the hydroalcoholic extract promoted action log reduction in population density or total inactivation forward all bacteria. Evidence shows that in addition to population density of confrontation, intrinsic characteristics of isolated individuals of both genders also interfered in the time required for the action to be promoted.

Os MÃtodos da DiluiÃÃo de Uso (MDU) e da SuspensÃo da AOAC sÃo oficialmente aceitos no Brasil na avaliaÃÃo da eficiÃncia antimicrobiana de desinfetantes, para fim de registro comercial. O estudo teve como objetivo determinar a eficiÃncia antimicrobiana das soluÃÃes de hipoclorito de sÃdio nas concentraÃÃes 5, 100 e 200mg/L e de cloreto de benzalcÃnio a 77 e 10.000mg/L e avaliar os processos de higienizaÃÃo das mÃos e botas dos manipuladores e das caudas de lagosta, como matÃria-prima, em um Entreposto de Pescado na cidade de Fortaleza-CE. Os testes aplicados aos desinfetantes foram MDU (INCQS/POP No65.3210.007) que utiliza as cepas padrÃo Staphylococcus aureus, Salmonella Choleraesuis e Escherichia coli e SuspensÃo (AOAC 960.09) que testa os desinfetantes contra S. aureus e E.coli. Foi verificada a eficÃcia dos procedimentos de higienizaÃÃo das mÃos e botas dos manipuladores e das caudas de lagostas atravÃs da reduÃÃo decimal (RD) das bactÃrias heterotrÃficas cultivÃveis (BHC), de amostras colhidas durante o processamento. Procedeu-se a contagem bacteriana atravÃs do mÃtodo de Contagem PadrÃo em Placas (CPP) pelas tÃcnicas pour plate (mÃos e botas) e spreadplate (caudas), utilizando-se Ãgar Extrato Glicose Triptona (TGEA), e os resultados foram expressos em UFC/mÃo, UFC/cm2 e UFC/cauda. As soluÃÃes de hipoclorito de sÃdio foram reprovadas pelo MDU nas concentraÃÃes 5, 100 e 200 mg/L e aprovadas (RD > 5 ciclos logarÃtmicos) em pelo menos uma repetiÃÃo para o teste da SuspensÃo. As soluÃÃes do cloreto de benzalcÃnio foram aprovadas nos dois testes apresentando melhor eficiÃncia microbiolÃgica para o teste de suspensÃo (RD >5 ciclos logarÃtmicos) na concentraÃÃo 10.000 mg/L. As RD das mÃos e das botas dos manipuladores e das caudas de lagosta foram respectivamente < 0,8,< 0,6 e < 0,6 ciclo logarÃtmico. O MDU e o MÃtodo da SuspensÃo mostraram concordÃncia parcial na aprovaÃÃo antimicrobiana das soluÃÃes tendo esse Ãltimo maior nÃmero de aprovaÃÃes. A carga microbiana inicial definiu a boa eficiÃncia do processo de higienizaÃÃo para a matÃria-prima e mÃos e a ineficiÃncia para as botas no Entreposto de Pescado. As RD encontradas para as cepas padrÃo (Staphylococcus aureus e Escherichia coli), analisadas pelo mÃtodo da SuspensÃo testando hipoclorito de sÃdio e cloreto de benzalcÃnio foram maiores do que aquelas RD encontradas para BHC apÃs o processo de higienizaÃÃo das mÃos, botas e caudas de lagosta. / The AOAC use-dilution method (UDM) and suspension method are officially accepted in Brazil for the evaluation of the antibacterial efficiency of disinfectants when applying for product registration. The purpose of this study was to determine the antibacterial efficiency of solutions of sodium hypochlorite at 5, 100 and 200mg/L and benzalkonium chloride at 77 and 10,000mg/L, and to evaluate the procedures employed to disinfect raw material (lobster tails) and the hands and boots of handlers at a seafood processing facility in Fortaleza city. The disinfectants were tested with UDM (INCQS/POP No65.3210.007) using standard strains of Staphylococcus aureus, Salmonella Choleraesuis and Escherichia coli, and with the suspension method (AOAC 960.09) using S. aureus and E. coli. The hygienization procedures for hands, boots and raw material were evaluated by decimal reduction (DR) of aerobic mesophiles in samples collected during processing. The bacteria were quantified by standard plate count (pour platefor hands and boots; spread plate for tails) in Tryptone Glucose Extract agar, and results were expressed as CFU/hand, CFU/cm2 and CFU/tail. The sodium hypochlorite solutions failed the UDM test at all concentrations, but were approved (DR >5) in the suspension test for at least one repetition. The benzalkonium chloride solutions were approved with both methods, with the best performance observed for the suspension method (DR >5log) at 10.000 mg/L. DR values were <0.8 (hands), <0.6 (boots) and <0.6 (tails). The two methods (UDM and Suspension) were in partial agreement regarding the approval of solutions, but the latter method yielded a greater number of approvals. Due to differences in the initial bacterial load, the hygienization procedures were efficient for hands and lobster tails but inefficient for boots. DR values were significantly higher for standard strains (S. aureus and E. coli) tested with the suspension method using sodium hypochlorite and benzalkonium chloride than for aerobic mesophiles following the hygienization of hands, boots and lobster tails sampled at the seafood processing facility.