Submitted by PPG Zoologia (zoologia-pg@pucrs.br) on 2018-06-29T17:28:48Z No. of bitstreams: 1 Dissertac?a?o_Mestrado_Raphaela_ (003).pdf: 1487803 bytes, checksum: 85e14999295df94278920f21e8362c4d (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-07-09T11:35:44Z (GMT) No. of bitstreams: 1 Dissertac?a?o_Mestrado_Raphaela_ (003).pdf: 1487803 bytes, checksum: 85e14999295df94278920f21e8362c4d (MD5) / Made available in DSpace on 2018-07-09T11:42:43Z (GMT). No. of bitstreams: 1 Dissertac?a?o_Mestrado_Raphaela_ (003).pdf: 1487803 bytes, checksum: 85e14999295df94278920f21e8362c4d (MD5) Previous issue date: 2018-02-26 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / The increase of environmental pollutants sources directly affects the ecosystem and human health, mainly due to loss of soil and water quality. Among these toxic agents are included endocrine disruptors, which can cause changes in biochemical pathways and physiological changes in organisms. Studies with different forms of bisphenols, compounds present in many products of human consumption and the environment, had already demonstrated extremely adverse effects on behavior and gene expression of targets related to neural and endocrine systems during development. The zebrafish, Danio rerio, is an excellent model organism for studying neurotoxicity, due to its advantages with respect to specific aspects of their development and a low maintenance cost when compared to other vertebrate model organisms. This work proposes the investigation of the effects of bisphenol A (BPA), its chlorinated derivatives and its substitutes (BPS and BPF), toxicological and behavioral parameters combined with characterization of its potential underlying cellular and molecular mechanisms such as cell death and proteins and gene expression in the nervous and endocrine systems. After the embryo and larvae were exposed to different concentrations of BPA and its derivatives, morphological analyzes and embryo survival were carried out, as well as behavioral tests of locomotion and cognitive evaluation of the larvae. In the concentrantion curve of the different bisphenolic compounds, no significant difference was observed in the survival, teratogenesis and hatching of the animals, however, a significant increase was detected in the exploratory parameters of the BPA and BPF groups. When comparing the aversive behavior of the BPA with the DMSO, there was no statistical difference between the groups, whereas in the BPS an improvement in the escape response of the larvae occurred. Additionally, behavioral and locomotor activity analyzes will be performed in adult animals previously treated with the compounds studied during the initial development. In this way, it is expected to contribute to the current knowledge of neurotoxicity mechanisms related to endocrine disruption caused by contaminants of the bisphenol family. / O crescimento das fontes de poluentes ambientais afeta diretamente o ecossistema e a sa?de humana, principalmente devido ? perda da qualidade do solo e da ?gua. Entre estes agentes t?xicos, est?o inclu?dos os disruptores end?crinos, os quais podem acarretar diversas mudan?as em vias bioqu?micas e altera??es fisiol?gicas em organismos. Estudos com diferentes formas de bisfen?is, compostos presentes em diversos produtos de consumo humano e inclusive no ambiente devido ? contamina??o por efluentes industriais, j? apresentaram efeitos extremamente nocivos na regula??o da express?o de genes relacionados ao sistema neural e end?crino durante o desenvolvimento, al?m de altera??es comportamentais severas. O zebrafish, Danio rerio, ? um excelente organismo modelo para estudar neurotoxicidade, uma vez que possui vantagens em rela??o a aspectos espec?ficos do seu desenvolvimento e um custo reduzido de manuten??o quando comparado a outros vertebrados modelos. Este projeto prop?s o estudo dos efeitos do bisfenol A (BPA), seus derivados clorados e substitutivos (BPS e BPF), sobre par?metros de embriotoxicidade e comportamento, buscando tamb?m caracterizar os mecanismos celulares e moleculares dos potenciais efeitos observados avaliando morte celular e n?veis de prote?nas e express?o de genes alvos em embri?es e larvas de zebrafish. Para tanto, ap?s a exposi??o dos embri?es e larvas a diferentes concentra??es de BPA e seus derivados, foram realizadas an?lises morfol?gicas e sobreviv?ncia dos embri?es; ensaios comportamentais de locomo??o e avalia??o cognitiva das larvas. Ao realizar a curva de concentra??o dos diferentes compostos bisfen?licos, n?o foi observada diferen?a significativa na sobreviv?ncia, teratog?nese e eclos?o dos animais, por?m, ao analisar o comportamento explorat?rio dos animais tratados com BPA e BPF foi detectado um aumento significativo nos par?metros explorat?rios das larvas. Ao compararmos o comportamento aversivo do BPA com o controle tratado com o ve?culo DMSO, n?o existiu diferen?a estat?stica entre os grupos, enquanto no BPS ocorreu uma melhora na resposta de escape das larvas. Adicionalmente, ser?o realizadas an?lises comportamentais e de atividade locomotora em animais adultos previamente tratados com os compostos estudados durante o desenvolvimento inicial. Desta forma, espera-se contribuir para o conhecimento atual dos mecanismos de neurotoxicidade relacionados ? disrup??o end?crina causada por contaminantes da fam?lia dos bisfen?is.

Submitted by PPG Comunica??o Social (famecos-pg@pucrs.br) on 2018-10-16T13:12:39Z No. of bitstreams: 1 TIAGO_DA_COSTA_GUIMAR?ES_DIS.pdf: 2281002 bytes, checksum: 0afcc652ef689015dd4a37e88d93fbce (MD5) / Approved for entry into archive by Sheila Dias (sheila.dias@pucrs.br) on 2018-10-17T14:44:02Z (GMT) No. of bitstreams: 1 TIAGO_DA_COSTA_GUIMAR?ES_DIS.pdf: 2281002 bytes, checksum: 0afcc652ef689015dd4a37e88d93fbce (MD5) / Made available in DSpace on 2018-10-17T14:55:11Z (GMT). No. of bitstreams: 1 TIAGO_DA_COSTA_GUIMAR?ES_DIS.pdf: 2281002 bytes, checksum: 0afcc652ef689015dd4a37e88d93fbce (MD5) Previous issue date: 2018-08-27 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This essay is a study of the impacts of disruptive organizations, taking as case study the Uber Technologies, defined for this research as a disruptive company, choosed for being one of the most prominent and controversial within the present-day capitalism?s new shape of own business. Uber Technologies communication practices are evidenced and discussed as an innovative organization, whose discourse is constructed according to their stakeholders. Therefore, its tactics include charm the printed media using the innovative speech and the quality improvement of the public transportation service, which can be reached through competition dynamics. This way, it is assumed that this model follower companies are ruled for an unbridled quest for innovation, causing market ruptures and pre-made social models, even analyzing, thereupon, the social impacts provoked by Uber Technologies. The research has a bibliographic and documentary character. The bibliographic character based on the disruption and creative destruction, based on the works of Christensen (2001), Schumpeter (1961) and Harvey (2011). The similarities of virtuality and ubiquity with the concepts of place and non-place, are presented from the works of ?uge (1994), L?vi (1996,1999), Santaella (2007) and Santos (2008). We also reviewed different perspectives of the organizational studies, drawing on the works of Baldissera (2010), Chanlat (2010), Freitas (1999), Hall (2006), Han (2017), Kunsch (2008), Mumby (2013), Schiraro (2004) and Srour (2012). The analysis of the Uber news published in the newspapers Zero Hora and Correio do Povo in the period from november 2015 to april 2018 showed at first the disruptive impact of the organization and, later, its trajectory and regulation. In turn, the Uber advertising campaign sought to humanize the relations between passengers/customers and drivers, representing situations of lightness and good acquaintanceship provided by the application. The presence of Uber Technologies is recent in the world, and with its maturation, new guidelines can become relevant in the eyes of the media organizations, acquiring more space in the social agenda. / A presente disserta??o trata sobre os impactos das organiza??es disruptivas, tendo como estudo de caso a Uber Technologies, justificando-se sua escolha por ser uma das empresas mais expoentes e controversas desse novo modelo de neg?cio pr?prio do capitalismo contempor?neo. Evidencia-se e discute-se as suas pr?ticas comunicacionais enquanto organiza??o inovadora, cujo discurso ? constru?do acordo com seus p?blicos de interesse. Suas estrat?gias incluem a sedu??o dos meios impressos, atrav?s do discurso da inova??o e da melhora na qualidade do servi?o de transporte, que pode ser atingido atrav?s das din?micas de competi??o. Assim, parte-se do pressuposto de que empresas que seguem esse modelo s?o pautadas por uma busca desenfreada pela inova??o e causam rupturas de mercados e modelos sociais preestabelecidos, analisando-se, em raz?o disso, tamb?m, os impactos sociais provocados pela Uber Technologies. A pesquisa de car?ter bibliogr?fico e documental, reflete sobre a tem?tica da disrup??o e destrui??o criativa, embasadas nas obras de Christensen (2001), Schumpeter (1961) e Harvey (2011). As semelhan?as de virtualidade e ubiquidade com os conceitos de lugar e n?o lugar s?o apresentadas a partir das obras de ?uge (1994), L?vi (1996,1999), Santaella (2007) e Santos (2008). Tamb?m foram revisadas diferentes perspectivas dos estudos organizacionais, apoiando-se nas obras de Baldissera (2010), Chanlat (2010), Freitas (1999), Hall (2006), Han (2017), Kunsch (2008), Mumby (2013), Schiraro (2004) e Srour (2012). A an?lise das mat?rias sobre a Uber veiculadas nos jornais Zero Hora e Correio do Povo, no per?odo de novembro de 2015 a abril de 2018, evidenciou num primeiro momento, o impacto disruptivo da organiza??o, e posteriormente, a sua trajet?ria e regulamenta??o. Por sua vez, a campanha publicit?ria da Uber procurou humanizar as rela??es entre passageiros/clientes e motoristas, representando situa??es de leveza e boa conviv?ncia proporcionadas pelo aplicativo. A presen?a da Uber Technologies ? recente no mundo, e com seu amadurecimento, novas pautas podem tornar-se relevantes diante dos olhos das organiza??es midi?ticas, ganhando maior espa?o na pauta social.