Thesis (MscAgric (Horticulture))--University of Stellenbosch, 2006. / Protea, Leucadendron and Leucospermum belong to the Proteaceae family. These three genera form an important part of the floriculture industry in South Africa and are commonly used as fresh cut flowers or dried flowers for both the local and international market. The distribution of macro and micro - nutrients was investigated in Protea ‘Cardinal’ using rooted cuttings grown from October 2001 to March 2002. The plants were divided into 1st flush leaf and stem, 2nd flush leaf and stem, basal leaf and basal stem, roots and the flower bud. These tissues were analysed to determine N, P, K, Ca, Mg, Fe, S, Na, B and Mn concentration. Results indicated that N decreased over time in all tissues and accumulated more in leaves than in stems. P in leaves and stems increased with time, while K increased in leaf tissues and remained stable in stem tissue. Ca did not change in young leaves but was high in old leaves. Mg in leaves increased but in basal stem, 1st flush stem and roots Mg decreased over time. Fe in leaves and roots increased with time and not a less significant change occurred in stems. In most tissues, B and Na decreased with time. S increased in leaves and decreased in stems with time. Mn was relatively stable in most tissues except in basal leaves where it increased. Nutrient concentration in tissues, especially in leaves, showed no distinct pattern in the distribution of nutrients. Eleven cultivars from the three genera, namely Protea, Leucadendron and Leucospermum, were used to develop deficiency symptoms of macro - nutrients by using different nutrient compositions. The plants were grown in 20cm pots from December 2002 to September 2003. The eleven cultivars were chosen because of their high market value. Visual symptoms were recorded in two stages with a five - month interval for each stage. The first stage was recorded from December 2002 to April 2003 and the second stage was recorded from May 2003 to September 2003. Observed symptoms indicated significant differences between the control and treatments in which specific nutrients were withheld. Some cultivars exhibited some symptoms that are commonly noticed under field conditions, e.g. in the N deficiency treatment the upper leaves of Protea ‘Sylvia’ were reddish and the lower leaves were chlorotic especially at the later stage. Protea ‘Red Rex’ in the P deficiency treatment had random red tints around the leaf including the petiole, these symptoms are also commonly noticed in Protea ‘Red ‘Rex’ in field conditions. Leucadendron ‘Rosette’ also showed some uncommon symptoms of accumulating “sugar” on leaf tips in Ca deficiency treatment that was not observed in any other cultivar. In some cultivars the symptoms were systematic e.g. Leucadendron ‘Chameleon’ while in other cultivars the deficiency affected a certain leaf age e.g. Leucospermum ‘High Gold’ in the Ca deficiency treatment. The buds in 'High Gold' died prematurely in the Ca deficiency treatment. Protea ‘Cardinal’ was used to determine the optimal N and P source and concentrations for optimal growth. Protea ‘Cardinal’ was grown in a temperaturecontrolled glasshouse for seven months using silica sand as growth medium. Different levels and sources of N and P were applied. The N was applied in a complete nutrient solution as NH4 +, NO3 -, NH4 +: NO3 - (1: 1, 1: 4 and 4: 1 ratios) and Urea, at different concentration levels: 5 mM, 1 mM and 0.1 mM. P was applied at 1 mM, 0.1 mM and 0.01 mM. The plants were fertigated manually with 1L nutrient solution on every second day of the week. P at 0.01 mM and 0.1 mM resulted in optimal plant growth. The 1 mM P resulted in marginal leaf scorching or dryness. When N was applied at 5 mM plant growth was more optimal than when N was applied at 1 mM and 0.1 mM. More dry weight was accumulated at 5 mM than at 1 mM or 0.1 mM N. The dry weight of leaves, stem and roots tissues at 5 mM were higher in the NO3 - treatment.

Thesis (MScAgric (Horticulture))--University of Stellenbosch, 2009. / ENGLISH ABSTRACT: In order to develop a novel pink- or red-fleshed apple for the fresh consumer market,the red-fleshed genotype, ‘KAZ 91’ (Malus niedzwetzkyana Dieck.), was crossed with ‘Meran’ (M. domestica Borkh) at the Agricultural Research Council (ARC) apple breeding programme in South Africa. The objective of this study was to evaluate and quantify colour variability in the flesh and peel of this progeny (Family 1), to assess quality traits (i.e., acidity and total soluble solids) and phenolic levels in fruit peel and flesh, and to investigate consumer preference for the taste and appearance of redfleshed apples. All data, except for consumer preference, were also collected on two white-fleshed M. domestica Borkh families, i.e., ‘Reinette Burchardt’ x ‘Treco Red Gala’ (Family 2) and ‘Meran’ x ‘Treco Red Gala’ (Family 3). Fruit of Family 1 seedlings were on average darker red with greater blush coverage and higher anthocyanin and phenolic levels compared to fruits of Family 2 and 3. The proportion of bearing trees with red-fleshed fruits in Family 1 increased from 25% in 2007 to 35% in 2008. The intensity and distribution of red pigmentation in the flesh varied considerably between seedlings and even between individual apples from the same tree. A high intraclass correlation coefficient was found for red-flesh coverage within Family 1, indicating a high level of genetic determination that can be used in breeding. When only red-fleshed seedlings were considered, an intermediate repeatability coefficient (0.54) for red-flesh coverage indicates that the extent of red flesh coverage varies to some extent between seasons. The effect of environmental factors on red flesh colour needs to be assessed and breeders need to take care to select for genotypes with stable flesh colour intensity and coverage. Small intraclass correlations were found between families for other traits. No correlation was found for anthocyanin and total phenolics in both peel and flesh or between anthocyanin levels in the peel and flesh of Family 1 fruit. This suggests that red-fleshed fruit will not necessarily be high in antioxidants – since phenolics is by far the greatest contributor to fruit antioxidant capacity. The lack of a correlation between peel and flesh anthocyanin levels also suggest that fruit with dark red flesh will not necessarily have a dark red skin colour. Family 1 fruit were more prone to flesh browning and were more acidic compared to fruit of Families 2 and 3. With regard to consumer preference for flesh colour, 74% of South African consumers preferred white flesh while 64% preferred an attractive “floral” pattern created by the combination of a red cortex and white core. Consumers indicated a much lower liking for other distribution patterns and lower intensities of red flesh colour. Consumers preferred the taste of apples that were crisp, crunchy and high in apple flavour irrespective of flesh colour. Red-fleshed fruit were generally acidic or had poor texture, and some were also astringent. However, despite a general dislike in acidic fruit, consumers showed a preference for acidic fruit if that fruit also had high red-flesh coverage. / AFRIKAANSE OPSOMMING: Appeltelers van die Suid-Afrikaanse Landbounavorsingsraad (LNR) Infruitec- Nietvoorbij poog om unieke appelkultivars met ‘n pienk of rooi vleiskleur te ontwikkel. Vir hierdie doel het hulle die rooivleis genotipe, ‘KAZ 91’ (Malus niedzwetzkyana Dieck), gekruis met ‘Meran’ (M. domestica Borkh). Hierdie studie is uitgevoer ten einde kleurvariasie, interne kwaliteitseienskappe (i.e., suurheid en totale oplosbare vastestowwe) en vlakke van fenole in die vleis en skil van bogenoemde kruisingkombinasie se nageslag (Familie 1) te evalueer en te kwantifiseer asook om verbruikersvoorkeure vir die smaak en voorkoms van rooivleis appels te bestudeer. Buiten vir verbruikersvoorkeure, is alle data ook ingesamel vir twee witvleis M. domestica families, naamlik ‘Reinette Burchardt’ x ‘Treco Red Gala’ (Familie 2) en ‘Meran’ x ‘Treco Red Gala’ (Familie 3). Familie 1 saailinge se vrugte was gemiddeld donkerder rooi met ‘n groter rooi blos en hoër antosianien- en fenoolvlakke in vergeleke met vrugte van Families 2 en 3. Van die Familie 1 saailinge wat wel vrugte gedra het, het 25% en 35% in onderskeidelik 2007 en 2008 vrugte met rooi vleis gehad. Die intensiteit en verspreiding van rooi pigmentasie in die vleis het aansienlik varieer tussen saailinge en selfs tussen individuele appels van dieselfde boom. ‘n Hoë intraklas korrelasie koëffisiënt is gevind vir die proporsie van die vleis met rooi pigmentasie in Familie 1 nageslag. Dit dui op ‘n hoë vlak van genetiese determinasie en vinnige vordering met teling vir hierdie eienskap. Indien net rooi-vleis saailinge egter oorweeg word, word ‘n intermediêre herhaalbaarheid koëffisiënt (0.54) vir die proporsie van die vleis wat rooi is verkry, wat dui op aansienlike variasie tussen seisoene in die omvang van rooi pigmentasie. Die effek van omgewingsfaktore op rooi vleiskleur behoort dus bestudeer te word en telers moet let daarop om te selekteer vir genotipes met stabiele vleiskleur intensiteit en bedekking. Die intraklas korrelasies tussen families vir ander vrugeienskappe was klein. Antosianienvlakke en totale fenole in die skil en vleis van Familie 1 vrugte het nie gekorreleer nie. Dit dui daarop dat rooivleis appels nie noodwendig ‘n hoër antioksidantkapasiteit het nie – fenole maak by verre die grootste bydrae tot die antioksidantkapisiteit van vrugte. Antosianienvlakke in die skil en vleis van Familie 1 vrugte het ook nie gekorreleer nie wat daarop dui dat vrugte met ‘n donker rooi vleis nie noodwendig ook ‘n donker skilkleur sal hê nie. Familie 1 vrugte was gemiddeld suurder as vrugte van Families 2 en 3 en Familie 1 vrugte se vleis het gemiddeld ook meer verbruining ondergaan. Ten opsigte van verbruikersvoorkeur vir vleiskleur is gevind dat 74% van verbruikers ‘n wit vleiskleur verkies het terwyl 64% gehou het van die aantreklike “blom” patroon gevorm deur ‘n rooi korteks en wit kern. Verbruikers het aansienlik minder gehou van enige ander verspreiding van rooi pigment in die vleis of van ‘n laer intensiteit rooi kleur. In terme van smaak is geurige appels met ‘n bros tekstuur verkies ongeag hul vleiskleur. Rooivleisappels was oor die algemeen suur met ‘n swak tekstuur. Sommige rooivleisappels was ook frank. Tog, ten spyte van ‘n algemene afkeur in suur appels, het verbruikers ‘n voorkeur getoon vir vrugte met ‘n kombinasie van hoë suur en ‘n hoë proporsie vleis met rooi pigmentasie.

Thesis (MSc (Horticulture))--University of Stellenbosch, 2007. / This study was undertaken to quantify some of the effects of daily fertigation on ecophysiological responses in citrus trees (Citrus spp.). Initial research was conducted to optimise and standardise the sampling procedure to quantify stem water potential (ψstem) in citrus trees. To reliably determine the plant water status of citrus trees, the following conditions are required to minimise unwanted variation in ψstem measurements. Bagging of leaves with black polyethylene envelopes covered with aluminium foil 3 to 4 hours prior to measuring ψstem allows the plant water status in those leaves to equilibrate with whole-tree plant water status, thereby providing a realistic measurement of the current water status. The use of aluminium foil to cover the bagged leaves, reduces unwanted heat stress by reflecting sunlight, and dramatically reduced variation in ψstem. The time of day at which ψstem measurements are made is important to ensure consistency in comparisons among treatments and interpretation of irrigation treatment effects. “Physiological midday” is the preferred time of day to measure ψstem, i.e. 1100 HR. Transpiring leaves with open stomata would be in sun-exposed positions on the east side of trees and should be used for making ψstem measurements. Under similar experimental conditions as those used here, only three leaves per replicate are required to detect a difference of 0.05 MPa in ψstem between treatment means. Plant water status categories were developed which may have useful practical applications, i.e. >-1.0 MPa = no water deficit; -1.0 to -1.2 MPa = low water deficit; -1.2 to -1.4 MPa = moderate water deficit; <-1.4 to -1.6 MPa = high water deficit; and <-1.6 MPa = severe water deficit. Attempts are being made to develop systems that improve crop management and enhance citrus fruit production through efficient and timeous application of water and mineral nutrients which has led to the use of daily drip fertigation or the open hydroponics system (OHS). However, the perceived benefits are not necessarily supported by facts. Fruit size and yield are apparently enhanced, but possible negative aspects of the system have not been quantified. Fruit produced on trees grown under daily drip fertigation generally have a lower total soluble solids concentration than on trees under micro-sprinkler irrigation. This is mainly due to a dilution effect that is caused by the greater availability of water and the uptake thereof. Sugar accumulation can be optimised by controlling the amount of water that the plant receives at different developmental stages. Therefore, it is essential to quantify the ecophysiological responses and benefits of OHS/daily fertigation, as well as the effects of this technology on fruit quality. ‘Nules Clementine’ mandarin (C. reticulata Blanco) trees in two commercial orchards in Simondium, Western Cape province, South Africa, received differential irrigation treatments. The treatments were applied at the end of stage I (± mid December) of fruit development. Stem water potential, fruit size and internal fruit quality were determined. Water-deficit stress enhanced sugar accumulation of ‘Nules Clementine’ mandarin by 0.3 to 0.6 °Brix under certain conditions. These conditions require that the difference in ψstem should be of a sufficient intensity of between 0.16 and 0.3 MPa, and this difference should be maintained for a sufficient duration of between 4 and 6 weeks. Furthermore, deficit irrigation should be applied relatively early in fruit development, namely during the sugar accumulation stage which starts within 4 weeks of the end of the fruit drop period and continues until harvest.

Thesis (MScAgric (Horticulture))--Stellenbosch University, 2008. / Chemical thinning of fruit trees has become a central management practice for ensuring high fruit quality at harvest and return bloom the following season. Three trials were conducted in the 2004/5, 2006/7 and 2007/8 seasons to investigate the efficacy and mode of action of chemical thinning agents on European pear cultivars (Pyrus communis L) in the Western Cape, South Africa. The first trial was conducted in the 2004/5 and 2006/7 seasons to evaluate the efficacy of 50, 100 and 150 mg.l-1 6-benzyladenine (BA), and 30 and 40 mg.l-1 naphthylacetamide (NAD) on ‘Early Bon Chrétien’ pear. BA was more effective than NAD in reducing crop load and improving fruit size. Crop load decreased and fruit size increased with increasing rate of BA. BA significantly improved, whilst NAD failed to improve return bloom. In the second trial, three experiments were conducted in the 2006/7 and 2007/8 seasons to evaluate the efficacy of 100 to 200 mg.l-1 BA on ‘Forelle’ pear. The first experiment was conducted in the 2006/7 season where BA rates of 100, 125 and 150 mg.l-1 generally failed to reduce crop load or to improve fruit size and fruit size distribution and return bloom. The second experiment was conducted in the 2007/8 season where two BA rates, 150 and 200 mg.l-1 and a split-application of 3 x 50 mg.l-1 improved fruit size. The 200 mg.l-1 rate was the most effective treatment. BA did not improve fruit size distribution and return bloom. The third experiment was conducted in the 2007/8 season where the effect of rate and timing of BA applications was evaluated. Two rates, 150 and 200 mg.l-1 were applied 8, 11 and 17 days after full bloom (d.a.f.b.). There was no significant interaction between BA rate and application time. The 200 mg.l-1 rate and the 11 d.a.f.b. (i.e. 8 to 10 mm average fruit size) applications were more effective in reducing crop load, and improving fruit size. BA at 150 and 200 mg.l-1 and at all application times significantly improved return bloom relative to the control. From these trials we concluded that BA is a reliable thinner for ‘Early Bon Chrétien’ at rates of 100 or 150 mg.l-1. On ‘Forelle’, BA is not a reliable thinner and we recommended further trials with BA in combination with other thinning agents. In the third trial, three experiments were conducted in the 2007/8 season to investigate the mode of action and effect of BA application time on European pear cultivars. The effect of site of application, bourse shoot growth and fruit size at time of application on the efficacy of BA was evaluated. Results from the experiments on the effect of site of application and bourse shoot growth were inconclusive. In terms of fruit abscission, there was a significant interaction between BA application time and fruitlet size. Early BA applications (8 d.a.f.b.) were significantly more effective in promoting fruit abscission, than later (11 and 17 d.a.f.b.) applications. Smaller fruit (6 to 8 mm) were found to be more susceptible to BA-induced fruit abscission than bigger fruit (8 to 12 mm).

Thesis (MScAgric (Horticulture))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: Persimmon production is new to South Africa with about 700 ha planted to the dioecious, parthenocarpic Triumph cultivar since 1998. Little local expertise is available to assist growers in achieving high yields of high quality fruit and previous research has shown that recipes that are followed in Israel, from where ‘Triumph’ was introduced to South Africa, do not necessarily have any beneficial effect in South Africa. ‘Triumph’ orchards in South Africa are often late in reaching full production. Persimmon trees are generally vigorous and prone to excessive fruit drop, partly due to excessive vegetative growth, especially when young and grown on the very vigorous Diospyros lotus seedling rootstock. The first objective of this study was to evaluate the use of growth retardants and various severities of girdling to increase flower formation, fruit set and yield in vigorous, young ‘Triumph’ orchards. Scoring and girdling improved fruit set and yield in two such orchards and are recommended as tools to improve yield in ‘Triumph’ in South Africa. Strapping, prohexadione-Ca (P-Ca) and paclobutrazol (PBZ) did not increase yield whereas 5 mm bark removal was too severe a treatment and decreased fruit quality in the current season and yield in the following season. None of the treatments had an effect on flower formation or decreased vegetative growth. PBZ, especially as foliar spray, appears to advance fruit maturity. P-Ca at 125 mg L-1 and 250 mg L-1 induced phytotoxicity symptoms and decreased yields in both orchards. However, further research is required before P-Ca and PBZ are completely discarded as treatments to manage vigor in ‘Triumph’ persimmon in South Africa. In contrast to the negative effect of excessive vigor on fruit production, the profitability of orchards is dependent on the rapid growth of trees after planting in order to fill the allotted canopy volume and achieve full production as quickly as possible. Hence, the second objective of this study was to determine optimum levels of irrigation and fertilizer application rates to attain early, high yields in newly planted ‘Triumph’. Fertigation was applied at three levels, viz. ½X, 1X and 2X with 1X being the commercial standard application rate. Irrigation was also applied at these levels without addition of fertilizer. In addition, fertilizer was applied at 0X, ½X and 1X at 1X irrigation level. Tree size increased with an increase in water application rate. Yield also increased linearly with an increase in water application rate due to a linear increase in fruit size. Fertigation and ½X water as well as an increase in fertilizer application rate at 1X irrigation substantially delayed fruit ripening. Hence, careful management of fertilizer and water application rate could be used to extend the harvesting period and, therefore, the marketing window of South African ‘Triumph’. We recommend that the trial be continued for a further few seasons so that the effect of water and fertilizer application rates on fruit quality and storability can be assessed. Fruit set may also be affected as trees reach their mature size with a concomitant increase in shading. / AFRIKAANSE OPSOMMING: Persimmonverbouing is ‘n nuwe bedryf in Suid-Afrika met ongeveer 700 ha van die tweeslagtige, partenokarpiese Triumph cultivar wat sedert 1998 aangeplant is. Min plaaslike kundigheid is beskikbaar om produsente van raad te bedien oor hoe om te werk te gaan om hoë opbrengste van hoë kwaliteit te verkry. Vorige navorsing het getoon dat resepte wat ‘Triumph’ van Israel na Suid-Afrika gevolg het, nie noodwendig suksesvol hier toegepas kan word nie. ‘Triumph’ boorde in Suid-Afrika neig om lank te neem alvorens hul hul maksimum produksievermoë bereik. Persimmons is oor die algemeen baie groeikragtig en geneig tot hoë vrugval, deels as gevolg van hul geil groei, en veral terwyl hulle jonk is en op die uiters groeikragtige Diospyros lotus saailingonderstam geënt is. Die eerste doelwit van hierdie studie was om die invloed van groei inhibeerders en verskillende grade van strafheid van ringelering op blomvorming, vrugset en oesopbrengs in jonk, sterk-groeiende ‘Triumph’ boorde te evalueer. Insnyding en ringelering met ‘n handsaag het vrugset en oeslading in twee groeikragtige boorde verbeter en word aanbeveel as geskikte ingrepe om die oeslading van ‘Triumph’ te verhoog. Draad-ringelering, en aanwending van prohexadione-Ca (P-Ca) en paclobutrazol (PBZ) het nie die opbrengs verhoog nie terwyl die verwydering van `n 5 mm strook bas té aggresief was en die vrugkwaliteit in die seisoen van toediening en opbrengs in die daaropvolgende seisoen verlaag het. Geen van die behandelings het blomvorming geaffekteer of vegetatiewe groei verminder nie. Dit wil voorkom asof PBZ, veral as blaartoediening, vrugrypwording kan versnel. Blare het tekens van fitotoksisiteit getoon na aanwending van P-Ca teen 125 mg L-1 en 250 mg L-1. P-Ca het ook die opbrengs in beide boorde aansienlik verlaag. Verdere navorsing is egter nodig alvorens P-Ca en PBZ sondermeer verwerp word as behandelings om die groei van ‘Triumph’ te beheer. Die winsgewendheid van boorde is afhanklik daarvan dat bome aanvanklik vinnig groei ten einde die toegekende boomryvolume so spoedig moontlik te vul en sodoende so vinnig as moontlik hul vol produksievermoë bereik. Bogenoemde is natuurlik teenstrydig met die negatiewe effek van uitermatige geil vegetatiewe groei op vrugproduksie. Die tweede doelwit van hierdie studie was dus om die optimale vlakke van besproeiing en bemesting te bepaal wat die vroeë aanvang van hoë opbrengste in nuwe ‘Triumph’ boorde sal verseker. Vloeibare bemesting is in kombinasie met besproeiing teen drie vlakke toegedien nl. ½X, 1X en 2X met 1X die kommersiële standaard vlak van toediening. Besproeiing is ook teen hierdie vlakke toegedien sonder dat kunsmis bygevoeg is. Addisioneel hiertoe is bemesting ook toegedien teen ½X, 1X en 2X teen 1X besproeiing. Boomgrootte het toegeneem met `n toename in die vlak van besproeiing. `n Lineêre toename in vruggrootte met ‘n toename in die vlak van besproeiing het ‘n oorsaaklike lineêre toename in opbrengs tot gevolg gehad. Bemesting in kombinasie met besproeiing, ½X besproeiing sonder bemesting, asook `n toename in die bemestingsvlak by 1X besproeiing het vrugrypwording substansieel vertraag. Die omsigtige bestuur van bemesting- en besproeiingsvlakke kan moontlik gebruik word om die oesperiode, en dus die bemarkingsvenster, vir Suid-Afrikaanse ‘Triumph’ te verleng. Ons beveel aan dat die proef vir ‘n vêrdere aantal seisoene voortgesit word sodat die effek van bemesting- en besproeiingsvlakke op vrugkwaliteit en -houvermoë bepaal kan word. Verhoogde oorskaduwing soos wat bome van sekere behandelings hul toegekende spasie bereik en oorskry, kan ook in die toekoms ‘n invloed op vrugset uitoefen.

Thesis (MScAgric) -- University of Stellenbosch, 2000. / ENGLISH ABSTRACT: Postharvest berry split and abscission are prevailing physiological disorders that negatively impact on the quality of table grapes exported from South Africa. Inferior grape quality due to these disorders results in a considerable decline in consumer confidence in the branded product, which leads to a drop in demand, and consequently, lower prices. Since information concerning postharvest factors influencing postharvest berry split and abscission is limited, the search for reliable methods to adequately control these problems remains elusive. In an attempt to obtain the required information, the influence of harvest temperature, harvest maturity, perforated liners, field heat removal prior to packing, delay periods before and after packing, storage duration and the elevation of storage temperature on the development of berry split and abscission in 'Thompson Seedless' (Vitis vinifera Linnaeus) table grapes was investigated. Changes in abscission related factors during berry development, and the influence of pre-and postharvest ethylene inhibitors on the development of berry abscission in 'Waltham Cross' table grapes, was also studied. Berry split was aggravated by packing 'Thompson Seedless' grapes at high pulp temperatures of approximately 30°C, especially if the grapes were packed in non-perforated bags. The incidence of berry split could be reduced by between 80 and 90% by packing grapes in perforated instead of non-perforated liners. Perforated bags also reduced levels of S02 damage. However, due to significantly more moisture loss from grapes in perforated bags, compared to non-perforated bags, the risk of higher fruit and stem desiccation and softer berries existed. Optimum size and density of perforations needs to be determined to reduce berry split without excessive loss of moisture from the grapes, and S02gas from the air space surrounding the product. The influence of harvest temperature and liner type on berry abscission was not conclusive. Advanced maturity increased grape resistance to berry split. However, grapes harvested too mature were prone to stem desiccation and the development of Botrytis decay. The occurrence of berry abscission also appeared to increase with advanced harvest maturity. Consequently, to ensure optimal post-storage quality, 'Thompson Seedless' grapes should be harvested as soon as horticultural maturity has been reached, which appears to be at approximately 18°Brix. Field heat removal for 1.5 hours at 19°C prior to packing had no beneficial or adverse effect on berry split and abscission. Delay periods prior to packing aggravated berry abscission, but did not influence berry split significantly. Grapes delayed for 12 hours showed a significant increase in berry abscission and Botrytis decay, compared to grapes delayed for only 3 or 8 hours. Considering that the absence of fungal decay is the most important quality prerequisite in table grapes, it is of vital importance to pack grapes with as short a delay period as possible. Grapes packed in non-perforated liners and delayed for different durations after packing, before the onset of forced-air cooling (FAC), showed significant differences regarding the incidence of berry split. Grapes delayed for 18 hours had significantly higher levels of berry split directly after the delay period, compared to grapes delayed for 6 or 12 hours. No significant difference in berry abscission occurred between grapes delayed for different periods. To minimise the amount of berry split, FAC should be applied as rapidly as possible after the packing of grapes in non-perforated liners. Two storage related factors significantly influenced the incidence of berry split in 'Thompson Seedless' grapes during cold storage significantly, viz. the duration of storage at -O.soC,and the increase in temperature after low temperature storage. Berry split increased almost linearly with prolonged storage at -O.soC. An elevation of storage temperature from -O.soC to 10°C any time during the cold storage period, further aggravated the split problem. Consequently, the reduction of berry split in 'Thompson Seedless' table grapes during cold storage requires (a) the shortest possible cold storage period, and (b) good temperature management throughout distribution, from initiation of cooling until the final point of sale. The grape berry abscission potential, as quantitatively indexed by the measurement of the fruit removal force (FRF), showed significant changes during berry development of 'Waltham Cross' table grapes, from 27 to 111 days after full bloom (OAFB). This showed that at certain stages of fruit growth, 'Waltham Cross' grapes are more prone to berry abscission. At 27 OAFB, when the berries had an average diameter of 6.6mm, the grape bunches showed a significantly higher potential for berry abscission, compared to grapes sampled at a later stage. 'Waltham Cross' has inherently straggly bunches with bare shoulders. Therefore, any abscission during berry development will aggravate the problem. Consequently, it is of vital importance that any adverse factors such as moisture stress be avoided, especially during the period when 'Waltham Cross' grapes appear to be very susceptible to berry abscission. Of all parameters measured, moisture loss showed the best correlation with abscission. Grapes harvested with total soluble solids (TSS) of 12.3°Brix, 83 OAFB, had a significantly higher abscission potential than grapes harvested more mature. Therefore, by harvesting 'Waltham Cross' grapes at optimum maturity, at a TSS of approximately 16.4°Brix, berry abscission can be reduced to a great extent. It was evident that at veraison, the metabolism of grape berries changes drastically, and additional to the rapid increase in sugars and the rapid decrease in acidity, a decrease in FRF occurs. Preharvest sprays of ReTain™ (a derivative of aminoethoxyvinylglycine), which inhibits ethylene synthesis, showed no promise as a means to reduce postharvest berry abscission. A postharvest treatment with EthylBloc® (1-methylcyclopropene), which inhibits ethylene action, only reduced berry abscission during one season. / AFRIKAANSE OPSOMMING: Die fisiologiese defekte korrelbars en los korrels wat algemeen voorkom tydens opberging van sekere tafeldruif-kultivars, het 'n negatiewe invloed op tafeldruiwe wat uitgevoer word vanaf Suid-Afrika. Minderwaardige kwaliteit as gevolg van hierdie defekte het 'n aansienlike afname in verbruikers-vertroue tot gevolg wat aanleiding gee tot 'n ooreenkomstige afname in aanvraag en prys van die produk. Inligting rakende na-oes faktore wat die voorkoms van korrelbars en los korrels beïnvloed is beperk, en geen gewaarborgde metode bestaan om hierdie twee defekte volkome te beheer nie. In 'n poging om dié gewenste inligting te bekom, is ondersoek ingestel na die effek van oes-temperatuur, oes-rypheid, geperforeerde sakke, veldhitte verwydering voor verpakking, vertragingsperiodes voor en na verpakking, tydsduur van opberging, en die verhoging van die opbergingstemperatuur, op die voorkoms van korrelbars en los korrels by 'Thompson Seedless' (Vitis vinifera Linnaeus) druiwe. Daar is ook ondersoek ingestel na veranderings in afsnoering verwante faktore tydens korrel-ontwikkeling, en die invloed van vooren na-oes toedienings van etileen inhibeerders op die ontwikkeling van los korrels by 'Waltham Cross'tafeldruiwe. Korrelbars is vererger deur 'Thompson Seedless' met hoë pulptemperature van ongeveer 29.5°C te verpak, veral indien dit in 'n riie-geperforeerde sak verpak is. Die voorkoms van korrelbars kon tussen 80 en 90% verminder word deur 'Thompson Seedless' druiwe in geperforeerde sakke te verpak, in plaas van nie-geperforeerde sakke. Geperforeerde sakke het ook S02 skade op die druiwe verminder. Tog, as gevolg van betekenisvol meer vogverlies vanaf druiwe in geperforeerde sakke as vanaf druiwe in nie-geperforeerde sakke, bestaan die risiko van meer stingel-uitdroging en minder ferm korrels indien druiwe in geperforeerde sakke verpak word. Optimale grootte en digtheid van perforasies moet bepaal word om korrelbars te verminder, maar sonder oormatige vogverlies vanaf die druiwe en oormatige verlies aan S02. Die invloed van oes-temperatuur en sak-tipe op los korrels was nie oortuigend nie. Gevorderde oes-rypheid het die druif se weerstand teen korrelbars verhoog. Daarteenoor was druiwe wat té ryp geoes is, meer gevoelig vir stingel-uitdroging en Botrytis bederf. Dit wilook voorkom of die voorkoms van los korrels toeneem met gevorderde rypheid. Dus, om optimum kwaliteit na opberging te verseker, moet 'Thompson Seedless' geoes word sodra hortologiese rypheid bereik word, wat blyk om by 'n totale opgeloste vaste stof-inhoud (TOVS) van ongeveer 18°Brix te wees. Veldhitte verwydering voor verpakking, vir 1.5 uur by 19°C, het geen effek gehad op die voorkoms van korrelbars en los korrels nie. 'n Vertragingsperiode voor verpakking het die los korrel-probleem vererger, alhoewel dit geen betekenisvolle invloed op die voorkoms van korrelbars gehad het nie. Druiwe wat vir 12 uur voor verpakking vertraag is, het betekenisvol meer los korrels en Botrytis bederf getoon, in vergelyking met druiwe wat slegs 'n vertragingsperiode van 3 of 8 uur ondergaan het. Aangesien die afwesigheid van bederf die belangrikste kwaliteits-vereiste vir tafeldruiwe is, is dit van kardinale belang om druiwe so gou as moontlik na oes te verpak. Druiwe, verpak in nie-geperforeerde sakke, wat vir verskillende periodes vertraag is voor geforseerde-lug verkoeling, het betekenisvolle verskille getoon betreffende die voorkoms van korrelbars. Druiwe vertraag vir 18 ure voor verkoeling, het betekenisvol meer korrelbars getoon, soos gemeet onmiddellik na die vertragingsperiode, in vergelyking met druiwe wat slegs vir 6 of 12 ure vertraag was. Geen betekenisvolle verskille in los korrels het voorgekom tussen druiwe wat verskillende vertragingsperiodes ondergaan het nie. Om korrelbars te verminder, moet geforseerde-lug verkoeling so gou as moontlik na verpakking van druiwe in nie-geperforeerde sakke toegepas word. Twee opbergings-verwante faktore beïnvloed die voorkoms van korrelbars by 'Thompson Seedless' druiwe tydens koelopberging, naamlik die tydsduur van opberging by -O.soC,asook 'n styging in temperatuur vanaf -O.soC tot 1DoC. Korrelbars het feitlik liniêr toegeneem met verlengde opberging by -O.soC. 'n Styging in temperatuur vanaf -O.SoCtot 1DoCop enige tydstip gedurende die koelopbergingsperiode, het korrelbars verder vererger. Dus, om korrelbars by 'Thompson Seedless' tydens opberging tot die minimum te beperk, moet die tydsduur van opberging so kort as moontlik wees, en moet die koue ketting regdeur die distribusie-proses gehandhaaf word, vanaf inisiëring van verkoeling tot en met die uiteindelike verkoop van die produk. Die afsnoerings-potensiaal van druiwe, soos kwantitatief geïndekseer is deur meting van die vrug-verwyderings-vermoë (VVV), het betekenisvol verander gedurende korrel-ontwikkeling van 'Waltham Cross' tafeldruiwe, vanaf 27 tot 111 dae na volblom (DNVB). Dit het getoon dat 'Waltham Cross' druiwe by sekere stadiums van vrug-groei meer gevoelig is vir korrel afsnoering. By 27 DNVB, wanneer die korrels 'n gemiddelde deursnee van 6.6mm gehad het, het die druiwe 'n betekenisvolle hoër potensiaal vir afsnoering getoon, in vergelyking met druiwe wat op 'n latere stadium getoets is. 'Waltham Cross' is inherent geneig tot yl trosse met kaal skouers, gevolglik sal enige afsnoering tydens korrel-ontwikkeling die probleem vererger. Dus is dit van kardinale belang dat enige nadelige faktor, soos byvoorbeeld vogstres, vermy moet word, veral gedurende periodes wanneer dit wil voorkom of 'Waltham Cross' baie vatbaar is vir korrel afsnoering. Van al die parameters wat gemeet is, het vogverlies die beste korrelasie met korrel afsnoering getoon. Druiwe wat 83 DNVB, by 'n TOVS van 12.3°Brix geoes is, het 'n betekenisvol hoër potensiaal vir korrel afsnoering getoon, in vergelyking met druiwe wat ryper geoes is. Dus, deur 'Waltham Cross' druiwe by optimum rypheid te oes, by 'n TOVS van ongeveer 16.4°Brix, kan korrelbars in 'n groot mate verminder word. Tydens verelson, wanneer die metabolisme van die druiwe drasties verander, was daar gepaardgaande met die drastiese toename in TOVS en die drastiese afname in totale titreerbare sure (TSS), ook 'n afname in Voor-oes bespuitings met ReTain™, wat etileen sintese inhibeer, het geen potensiaal getoon om los korrels by 'Waltham Cross' te verminder nie. 'n Na-oes behandeling met EthyIBloc®, wat etileen werking inhibeer, het slegs korrel afsnoering in een van die seisoene effens verminder.

Thesis (MScAgric)--Stellenbosch University, 2000. / ENGLISH ABSTRACT: Red colour development on bi-coloured apples and pears ensures better prices for producers. The use of postharvest irradiation to improve colour has been successful on apples, and the objectives of this thesis were to optimise conditions during irradiation for apples and to evaluate the effects of irradiation on pears. 'Cripp's Pink' apples responded to postharvest irradiation with high-pressure sodium (HPS) lights by developing a red blush, whereas the response to irradiation with UV 8 plus incandescent lights was less effective. '8raeburn' apples held at -0.5°C for 4 or 8 weeks prior to irradiation showed a decrease in hue angle and an increase in anthocyanin concentration after 72 hours of irradiation with HPS lamps. In 'Forelle' pears treated in the same way, neither colour development nor anthocyanin synthesis was affected by irradiation. '8raeburn' and 'Cripp's Pink' apples picked weekly for 5 weeks until the optimum harvest date were irradiated with HPS lights. A significant increase in fruit colour was only measured in mature fruit of both cultivars. 'Cripp's Pink' apples were harvested from two production areas with different microclimates, namely, Ceres and Grabouw, and stored for 0, 2 or 5 days at -0.5 °C before irradiation for 120 hours at either 6°C or 20°C. Fruit from Ceres that were irradiated immediately after harvest developed better colour at 6 °C than at 20°C. The differences between fruit irradiated at the two temperatures were no longer significant after 5 days of cold storage prior to irradiation. Fruit from Grabouw consistently developed better colour when irradiated at 6°C than at 20°C. Colour development slightly after 5 days of cold storage prior to irradiation. In another experiment, fruit from both areas were stored at -0.5°C for 20 days before irradiation at either 6°C or 6/20°C. The fluctuating temperature regime resulted in decreases in hue angle of 70° and 65° for the fruit from Grabouw and Ceres, respectively. The decreases were smaller (±200) when fruit were irradiated at 6°C. The hue angle value of well-coloured 'Cripp's Pink' apples held at 3rC under HPS lights for 144 hours increased from 29.3° to 48.3°, and anthocyanin concentration decreased from 739.9 IJg·g·1to 283.6 IJg·g·1. Control fruit held at the same temperature in the dark did not show any change in hue angle value or anthocyanin concentration. 'Bon Rouge' and 'Red d' Anjou', two full red pear cultivars, irradiated with HPS lights for 72 hours, showed no significant changes in hue angle. 'Forelle' pears, harvested with or without attached leaves, were irradiated with HPS at two temperature regimes, 20°C and 200/6°C. The resulting decreases in hue angle were attributed to yellowing and not red colour formation. In conclusion, the response of apples to postharvest irradiation was affected by maturity and temperature, while pears failed to respond at all. / AFRIKAANSE OPSOMMING: Rooikleurontwikkeling van twee-kleur appels en pere verseker beter pryse vir die produsente. Na-oesbestraling om kleur te verbeter is al suksesvol uitgevoer op appels, en die doelwit van hierdie tesis was om die kondisies vir appels gedurende bestraling te optimaliseer en om die effek van bestraling op pere te evalueer. 'Cripp's Pink' appels het reageer op na-oesbestraling met hoëdruk-natriumligte (HDN) deur 'n rooi blos te ontwikkel, terwyl die reaksie op bestraling met UV-B plus gloeilamplig minder effektief was. 'Braebum' appels opgeberg by -O.5aC vir 4 of 8 weke voor bestraling het 'n afname in die kleurskakeringswaarde getoon, asook 'n toename in antosianienkonsentrasie na 72 uur se bestraling met HDN ligte. 'Forelle' pere wat dieselfde behandeling ontvang het, het geen kleurontwikkeling en geen antosianienontwikkeling getoon na bestraling nie. 'Braebum' en 'Cripp's Pink' appels wat weekliks geoes is vir 5 weke tot die optimum oesdatum is bestraal met HDN ligte. Slegs die volwasse vrugte van beide kultivars het 'n betekenisvolle toename in kleur getoon. 'Cripp's Pink' appels is geoes in twee produksie areas met verskillende mikroklimate, naamlik Ceres en Grabouw. Vrugte is opgeberg vir 0, 2 of 5 dae by -O.5aC voor bestraling vir 120 uur by of 6aC of 20aC. Vrugte van Ceres wat onmiddellik na oes bestraal is het beter kleur ontwikkel by 6aC as by 20aC. Kleurontwikkeling by vrugte bestraal by 6 of 20aC het nie verskil wanneer vrugte vooraf opgeberg was by -~5ac vir 5 dae nie. Vrugte van Grabouw het konstant beter kleur pntwikkel wanneer bestraling by 6°C eerder as 20°C plaasgevind het. In die volgende eksperiment, was beide die vrugte van Ceres en Grabouw vir 20 dae opgeberg by -o.soC voor bestraling by 6°C of 6°/20°C. Die flukturerende temperatuur regime het afnames van 70° en 65° in kleurskakeringswaarde getoon vir die vrugte van Grabouw en Ceres, respektiewelik. Die afname was kleiner (±200)wanneer vrugte by 6°C bestraal is. 'Cripp's Pink' appels wat goed gekleur was en opgeberg is by 3rC terwyl dit blootgestel is aan HDN ligte vir 144 uur, het 'n toename van 29.30 tot 48.3° getoon vir die kleurskakeringswaarde, en antosianienkonsentrasie het afgeneem van 739 I'g.g-1 tot 283.6 I1g.g-1. Die kontrole vrugte opgeberg by dieselfde temperatuur in die donker het geen verandering in beide die kleurskakeringswaarde of die antosianienkonsentrasie getoon nie. 'Bon Rouge' en 'Red d' Anjou', twee volrooi peerkultivars, is bestraal met HDN ligte vir 72 uur en het geen betekenisvolle verandering in kleur getoon nie. 'Forelle' pere, geoes met of sonder 'n aangehegte stingelsegment, is bestraal met HDN ligte by twee verskillende temperatuur regimes, nl. 20°C of 20/6°C. Die afname in kleurskakeringswaarde is aan vergeling toegeskryf eerder as aan rooikleurontwikkeling. Ter opsomming, die reaksie van appels op na-oes bestraling is beïnvloed deur rypheid en temperatuur, terwyl pere geen reaksie getoon het nie.

Thesis (MScAgric)--Stellenbosch University, 2001. / ENGLISH ABSTRACT: Sunburn is a major problem in the apple industry worldwide. A kaolin product, M-97- 009 (100% kaolin), originally developed for insect control, was evaluated for its efficacy in controlling sunburn on pome fruit. Trials were conducted over two seasons in two different areas of the Western Cape, South Africa. During the first season, 'Royal Gala', 'Fuji' and 'Granny Smith' apples were evaluated in the Koue Bokkeveld. The kaolin was mixed with water and applied to the trees by means of a hand-gun regularly throughout the season. In addition to the effect on sunburn, effects on yield, colour development, snout beetle damage and other defects were determined. Sunburn was reduced on all the cultivars tested, although not significantly on 'Granny Smith'. Inconsistent effects on yield parameters were observed. The applications significantly reduced red colour on 'Fuji', but this problem was rectified by an improved application technique the following season. During the second season, additional apple cultivars and 'Rosemarie' pears were included in the trials. The apple trials were conducted in the Elgin area, and the 'Rosemarie' trial in the Koue Bokkeveld. Surround™ (95% kaolin) was applied to the trees by means of a commercial "blower". The effects on sunburn, yield, colour development and fruit maturity were determined. In contrast to the previous season, sunburn was not reduced on any of the cultivars examined. Yield parameters were not affected except in the case of 'Royal Gala' where the number of fruit was increased, and 'Cripps' Pink' where the number of fruit was reduced. Fruit colour was not affected in the apple cultivars, but chroma of 'Rosemarie' pears was reduced, indicating a less intense colour. The kaolin applications had a variable effect on fruit maturity. The effect of the foliar applications on photosynthetic photon flux density (PPFD), photosynthetic rate, stomatal conductance and transpiration rate was determined. In addition to these spot measurements, photosynthetic light response curves were determined. Measurements were taken on both the inner and outer canopies The applications significantly reduced photosynthetic rates in the inner canopy and reduced the apparent quantum efficiency of leaves on the outer canopy. No significant effect on PPFD was found. It appears that the white coating reflects light and allows less light to penetrate the leaf, thus reducing photosynthesis. Surround™ treatments could not counteract the damaging effects of the high temperatures experienced in the Western Cape during this season and was not effective as a control measure for sunburn. This does not appear to be a commercially viable solution for the sunburn problem and it would be worthwhile to investigate the use of alternative options, such as evaporative cooling. / AFRIKAANSE OPSOMMING: Sonbrand is een van die grootste probleme wat wêreldwyd in die appelbedryf ondervind word. 'n Nuwe produk, M-97-009 (100% kaolien), is oorspronklik ontwikkel vir insekbeheer in geïntegreerde plaagbeheer, maar daar is beweer dat dit moontlik sonbrand op kemvrugte kan verminder. Proewe is oor twee seisoene uitgevoer in die Koue Bokkeveld en ook in die Grabouw-omgewing in die Wes-Kaap. Tydens die eerste seisoen is die effek van M-97-009 op 'Royal Gala', 'Fuji' en 'Granny Smith' appels beoordeel. Die kaolien is met water gemeng en deur die loop van die seisoen met 'n handspuit op die bome gespuit. Die effek van die produk op sonbrand, oesgrootte, vruggrootte, kleurontwikkeling, kalanderskade en ander defekte is bepaal. Sonbrand is op alle kultivars verminder, alhoewel nie betekenisvolop 'Granny Smith' nie. Die effek van kaolien op oesparameters was rue konsekwent nie. kleurontwikkeling op 'Fuji' benadeel, heel moontlik Die toedienings het as gevolg van die toedieningstegniek. Met verbeterde toedieningsmetodes die volgende seisoen, is kleurontwikkeling van 'Fuji' nie benadeel nie. Tydens die tweede seisoen is die effek van kaolien op nog appelkultivars en 'Rosemarie' pere ge-evalueer. Die appelproewe is in Elgin uitgevoer, terwyl die 'Rosemarie'-proefin die Koue Bokkeveld uitgelê is. Surround™ (95% kaolien) is met kommersiële spuitpompe toegedien. Weereens is die effek van die produk op sonbrand, oesgrootte, vruggrootte, kleurontwikkeling en vrugrypheid bepaal. Sonbrand is nie verminder nie. Vruglading tydens oes is nie betekenisvol beïnvloed nie, behalwe in die geval van 'Royal Gala' waar die vruglading verhoog is, en 'Cripps' Pink' waar daar minder vrugte op die gespuite bome was. Die Surround™ toedienings het geen uitwerking op kleur van appels gehad nie, maar het die chromawaarde van 'Rosemarie' pere verlaag, d.w.s die vrugkleur was minder intens. Die kaolienspuite het 'n uiteenlopende effek op vrugrypheid gehad. Fotosintetiese foton vloeddigtheid (FFV), fotosintesetempo, huidmondjieweerstand en transpirasietempo is gemeet en ligreponskurwes van beide die buitenste en binneste blaardak is bepaal. Die Surround™ toedienings het fotosintese van blare ill die binneste gedeelte van die blaardak verminder en die kwantumdoeltreffendheid van blare op die buitenste deel van die blaardak verminder. Geen betekenisvolle effek is op FFV gekry nie. Dit wil voorkom asof die wit laag kaolien op die bome lig weerkaats en veroorsaak dat minder lig na die blaar deurdring. Dit verminder dan die fotsintesetempo. Met die baie warm weer wat in die Wes-Kaap gedurende die tweede seisoen ervaar is, kon die SUITound™behandelings nie sonbrand verhoed nie. Dit wil voorkom asof Surround™ nie 'n baie doeltreffende oplossing in kommersiële boorde sal wees nie. Alternatiewe oplossings, soos byvoorbeeld evaporatiewe verkoeling, saloorweeg moet word.

Thesis (MScAgric) -- University of Stellenbosch, 2002. / ENGLISH ABSTRACT: The table grape industry is a major contributor to the South African economy, directly through foreign earnings from this predominantly export-based industry, as well as indirectly through the employment of thousands of people. It is a growing industry and consists of several production areas. The fastest growing table grape production area in South Africa is the Lower Orange River area, which produces some of the earliest grapes in the Southern Hemisphere. The biggest river in South Africa irrigates this area and it has an extreme climate characteristic of semi-desert areas. This area is considered to be optimal for the production of high quality, early, white seedless grapes. Previously, this area was predominantly planted to Sultanina vines for the purpose of raisin production. When seedless table grapes became a consumer preference, the producers very successfully converted their production practices to yield export quality seedless grapes from the established Sultanina vineyards. Extensive new plantings as well as re-plantings occurred in this area, also including newer cultivars from local and overseas breeding programmes. Being a viticultural and economical hot-spot, the Lower Orange River area is attracting much attention as a table grape production area and it also formed the backdrop to this study. The cultivar profile is changing in the area and it is projected that Sultana-, Regal-, Prime Seedless and Sugraone will be the four major early, white seedless cultivars in 2005. Based on this knowledge and prompted by a lack of information regarding production costs and general profitability of the new cultivars, this study was initiated in the form of a comparative analysis between the four mentioned cultivars spanning the early, middle and late regions of the Lower Orange River area. The approach used extracted information regarding cultural input costs (specifically labour as man-hours and the consequent costs) per manipulation performed in the vineyards. This approach is different from the more general method of obtaining input costs for a specific area based on combined mean values, often not distinguishing between cultivars. The specific aims of the study included a comparative analysis of input costs for production cultural practices per main manipulation action, as well as a comparative analysis taking into account productivity, value and extraordinary costs related to each of the four cultivars. To this end, 22 experimental plots were identified for use in the study. Collaboration of the production managers of each of the experimental plots were procured and information regarding production costs per manipulation and productivity of each cultivar and experimental plot were extracted from their own record keeping systems or from documents provided to the production managers. The value (price achieved) of the various cultivars for the 2001/2002 table grape season were put into perspective by using data from a survey which included information regarding payments for the various cultivars during the season in the Lower Orange River area. Information regarding fruit and vine royalties was obtained from the various plant breeders' rights holders of the various cultivars, where applicable. Primary descriptions of each experimental plot concerning general cultivation practices and information regarding the specific season were used to qualify results obtained from the various blocks. Several complicating factors impacted on the study and specifically the subsequent analyses of the results. Some of the factors were already identified as complicating factors in the planning stage of the study and were mostly linked to the recent introduction of two of the cultivars to the Orange River area. From the data gathered and the analyses performed it became clear that it would be difficult to discern significant differences (where significant is defined as PS0.10), but clear trends were observed and indications obtained. Based on the input cost analyses of this study it is proposed that mature Prime Seedless will have the highest labour input and cultural production cost of the four cultivars, followed by Sugraone. The labour input and the cost for the production cultural practices studied for young Prime Seedless vines were very high in comparison to the mature Sultana Seedless and Sugraone vines, especially for the canopy management and bunch manipulation actions. Prime Seedless was especially prone to the set of small and uneven berries, which lead to very high labour input requirements and subsequent cost for bunch manipulations. Sugraone is also known for the set of small and uneven berries in the Lower Orange River area, especially in difficult climatic seasons, also requiring high labour input for bunch manipulations. The initial indication is that mature Sultana- and Regal Seedless will require similar labour inputs for cultural production practices. The fact that Regal Seedless does not require expensive gibberellic acid (GA) applications, or girdling for thinning and berry enlargement purposes, is a tremendous advantage from a production cost point of view. Accordingly, initial indications are that Regal Seedless will have the lowest cultural production cost of the four cultivars. Regal Seedless was prone to the set of uneven berries during the year of study and accordingly it is suspected that this factor will ultimately determine the labour requirements and cultural production input cost, especially in difficult climatic seasons. The labour input and ultimately the cultural production cost for Sultana Seedless will be determined by the correct timing and concentration of the GA applications for thinning and berry sizing. Sultana Seedless and Sugraone produced high yields during the 2001/2002 table grape season in the Lower Orange River area. Yield information from the various experimental plots confirmed that there is little to choose between the two cultivars in terms of yield when cultivation conditions and practices are optimal. Large variation was observed in the yield results from the Regal- and Prime Seedless experimental plots. This is largely due to the recent introduction of the cultivars to the area and the consequent scarcity of blocks of these cultivars that are in full production. It was impossible to identify clear trends in terms of the future productivity of mature Regal- and Prime Seedless, but some indications of labour inputs could be extracted and qualified. Early maturing Prime Seedless and Sugraone performed very well in terms of price, especially in the harvest period prior to week 50. This advantage of high prices early in the season is, however, not always applicable to early cultivars in the later maturing regions of the Lower Orange River area. Later during the season, after week 50, when the supply of table grapes to the overseas markets has increased sharply, Sultana Seedless is usually the best performer in terms of price of the four cultivars. The ultimate price obtained by a cultivar is to a large extent determined by supply and demand, quality and acceptance of the specific cultivar. This study and its outcomes have a strong regional (Lower Orange River) and local (South Africa) impact and the specific results will undoubtedly be valuable to the producers, exporters and other role-players with vested interest in the cultivars studied or in table grape production per se. The methodology adopted in this study, however, is of broader interest and dearly shows the advantage of having detailed and qualified information regarding cultivation practices and bringing it in relation to the labour and consequent costs required per action. This should lead to more business intelligence and realistic planning on the producer side when decisions regarding the choice of a cultivar for a specific production area with a particular marketing scope have to be made. This study has also paved the way for similar studies, specifically with regard to the detailed description of the methodology that was established. Knowledge of the problems experienced in this study provides a useful reference for the planning and execution of similar studies. / AFRIKAANSE OPSOMMING: Die tafeldruifindustrie dra grootskaals by tot die Suid- Afrikaanse ekonomie: regstreeks deur middel van buitelandse valuta vanaf hierdie hoofsaaklik uitvoer-gebaseerde industrie, asook indirek deur werkverskaffing aan duisende mense. Dit is 'n vinnig groeiende industrie en bestaan uit verskeie produksie-areas waarvan die Benede-Oranjerivierarea, waar van die vroegste druiwe in die suidelike halfrond geproduseer word, tans die meeste groei toon. Die grootste rivier in Suid-Afrika vloei deur hierdie gebied wat deur uiterste klimaatstoestande, soortgelyk aan die van semi-woestyngebiede, gekenmerk word. Hierdie gebied is baie gunstig vir die produksie van hoë-gehalte, vroeë, wit pitlose druiwe. In die verlede is hoofsaaklik Sultanina vir die produksie van rosyne in hierdie gebied verbou. Namate pitlose tafeldruiwe voorkeur begin geniet het onder verbruikers wêreldwyd, het produseerders in die area hul verbouingspraktyke suksesvol aangepas vir die produksie van uitvoergehalte tafeldruiwe vanaf die grootskaalse, reeds gevestigde Sultanina-wingerde. Uitgebreide aanplantings en heraanplantings, wat nuwe cultivars van plaaslike en oorsese teelprogramme ingesluit het, is in hierdie gebied gedoen. Die vinnige groei in tafeldruifaanplantings en -uitvoere, asook die ekonomiese impak van die industrie in die Benede-Oranjeriviergebied, het die afgelope aantal jaar sterk op die voorgrond getree en het gevolglik gedien as agtergrond vir hierdie studie. Die cultivarprofiel in dié area is besig om te verander en volgens vooruitskattings gaan Sultana, Regal, Prime Seedless en Sugraone die vier prominente vroeë, wit, pitlose tafeldruifcultivars in 2005 wees. Gebaseer op hierdie feit en na aanleiding van 'n behoefte aan meer inligting met betrekking tot produksiekostes en algemene winsgewendheid van die nuwe cultivars, is 'n vergelykende studie aangaande die vier genoemde cultivars in die Benede-Oranjeriviergebied geloods. Die benadering wat gedurende die studie gevolg is, het inligting aangaande produksie-insetkoste (spesifiek arbeid in man-ure en gevolglike koste) per manipulasie onttrek. Hierdie benadering verskil van die meer algemene metodiek om insetkoste-inligting van 'n spesifieke area van gekombineerde gemiddelde waardes te verkry. Met so 'n benadering word gewoonlik geen onderskeid tussen cultivars getref nie. Die spesifieke doelwitte van hierdie studie het 'n vergelykende analise aangaande die insetkoste van die produksiepraktyke per hoofmanipulasie/aksie ingesluit, asook 'n analise waar produktiwiteit, waarde en buitengewone koste van die vier cultivars in ag geneem is. In totaal is 22 eksperimentele persele gebruik in die studie. Samewerking van die produksiebestuurders van die onderskeie esperimentele persele is verkry ten opsigte van die verskaffing van inligting oor produksiekoste per manipulasie, en die produktiwiteit per cultivar en eksperimentele perseel. Die produksiebestuurders het die nodige dokumente ontvang om die inligting te onttrek, of kon die inligting verskaf soos dit in hul rekordhoudingsisteem voorgekom het. Die waarde (prys behaal) van die onderskeie cultivars vir die 2001/2002-seisoen is in perspektief gestel deur gebruik te maak van 'n opname wat in die Benede Oranjeriviergebied plaasgevind het. Hierdie opname het inligting oor die uitbetalings van die onderskeie cultivars in die area vir die 2001/2002- seisoen ingesluit. Inligting rakende die stok- en vrugproduksie-tantieme is vanaf die onderskeie plantttelersregtehouers van die cultivars verkry. Primêre beskywings van die algemene verbouingspraktyke van elke eksperimentele blok en inligting oor die spesifieke seisoen is gebruik om die data wat vanaf die esperimentele persele verkry is, in perskektief te stel. Verskeie kompliserende faktore het die studie en die ontleding van data beïnvloed. Verskeie van hierdie faktore is reeds geidentifiseer met die beplanning van die studie en was meestal gekoppel aan die onlangse bekendstelling van Regal en Prime Seedless aan die Benede-Oranjeriviergebied. Na aanleiding van die data wat ingesamel en ontleed is, was dit duidelik dat dit moeilik sou wees om betekenisvolle verskille (waar "betekenisvol" as PS0.10 gedifinieer is) tussen die cultivars uit te lig, maar dat dit egter wel moontlik sou wees om aanvanklike indikasies en tendense te kry. Gebaseer op die insetkoste-ontleding van die studie blyk dit dat volwasse Prime Seedless die hoogste arbeidsinsetle en produksiekoste van die vier cultivars gaan hê, gevolg deur Sugraone. Die arbeidsinsetle en koste van die produksie-aksies wat van jong Prime Seedless bestudeer is, was baie hoog in vergelyking met volwasse Sultana Seedless- en Sugraone-stokke, veral ten opsigte van lowerbestuur en trosmanipulasies. Prime Seedless was veral geneig tot die set van klein, oneweredige korrels, wat tot baie hoë arbeidsinsetle en gevolglik koste vir trosmanipulasies gelei het. Sugraone is ook daarvoor bekend dat dit geneig is tot die set van klein, oneweredige korrels in die Benede-Oranjeriviergebied (veral in moeilike klimaatseisoene), wat gevolglik tot hoë arbeidsinstle vir trosmanipulasie lei. Die aanvanklike aanduiding is dat volwasse Sultana en Regal Seedless min of meer die dieselfde arbeidsinsetle vir verbouing sal vereis. Die feit dat Regal Seedless nie duur gibberelliensuur (GS)-behandelings vir blomtrosuitdunning of korrelvergroting benodig nie, is 'n enorme voordeel in terme van produksiekoste. Gevolglik is die aanvanklike aanduiding dat Regal Seedless die laagste produksieskoste van die vier cultivars sal hê. In die studiejaar was Regal Seedless egter geneig tot die set van onweredige korrels en gevolglik word verwag dat hierdie faktor uiteindelik die arbeidsinsetle en produsiekoste van die cultivar sal bepaal, veral in moeilike klimaatseisoene. Die arbiedsinsetle en produksiekoste van Sultana Seedless sal bepaal word deur die korrekte tydsberekening en konsentrasie van die GS-behandelings vir uitdunning en korrelvergroting. Sultana Seedless en Sugraone het gedurende die 2001/2002-seisoen hoë opbrengste in die Benede-Oranjeriviergebied geproduseer. Oesdata inligting van die onderskeie esperimentele persele het bevestig dat daar min te kies is tussen die twee cultivars in terme van produktiwiteit wanneer verbouingstoestande en -praktyke optimaal is. Groot variasie is egter waargeneem in die opbrengsresultate van die Regal en Prime Seedless. Dit is hoofsaaklik as gevolg van die onlangse bekendstelling van die twee cultivars in die area en dus ook die beperkte aantal blokke van die cultivars wat reeds in vol produksie was. Dit was dus onmoontlik om duidelike tendense in terme van die toekomstige produksie van volwasse Regal en Prime Seedless te identifiseer. Indikasies van arbeidsinsette en produksiekoste kon egter wel verkry word. Vroeg rypwordende Prime Seedless en Sugraone vaar baie goed in terme van die prys wat dit behaal, veral in die oesperiode voor week 50. Hierdie voordeel van hoë pryse behaal vroeg in die seisoen is egter nie altyd van toepassing op vroeë cultivars in die later rypwordende areas van die Benede-Oranjeriviergebied nie. Later in die seisoen (na week 50), wanneer die aanbod van tafeldruiwe op oorsese markte skerp toegeneem het, is Sultana Seedless gewoonlik die beste presteerder in terme van prys van die vier cultivars. Die uiteindelike prys wat deur cultivars behaal word, word tot 'n groot mate bepaal deur vraag en aanbod, kwaliteit en aanvaarding van die cultivar deur die verbruiker. Die studie en die uitkomste daarvan het 'n sterk streeks (Benede-Oranjerivier) en plaaslike (Suid-Afrika) impak, en die spesifieke resultate salongetwyfeld van waarde wees vir produseerders, uitvoerders en ander rolspelers met bestaande belange in die cultivars of vir tafeldruifproduksie as sulks. Die metodiek wat in hierdie studie gebruik is, is egter van breêr belang en wys duidelik die voordele daarvan om gedetailleerde en gekwalifiseerde inligting aangaande produksiepraktyke te hê, wat dit ook in verband bring met arbeid en gevolglike koste per aksie. Dit behoort te lei tot meer besigheidsintelligensie en realistiese beplanning deur die produseerder met betrekking tot cultivarkeuse vir 'n spesifieke produksiearea met 'n spesifieke bemarkings geleentheid. Hierdie studie het ook die weg gebaan vir soortgelyke studies, spesifiek ten opsigte van die gedetailleerde beskrywing van die metodiek wat gevestig is. Kennis van die probleme wat in hierdie studie ondervind is, kan dien as nuttige verwysing vir die beplanning en uitvoer van soortgelyke studies.

Thesis (MScAgric)--Stellenbosch University, 2003. / ENGLISH ABSTRACT: Protea cut flowers are exported worldwide but the vase life of some species and cultivars is considerably shortened by post-harvest leaf blackening. Research has established carbohydrate depletion to be positively correlated with this disorder. Consequently, a study had been made of the carbohydrate status of various species and cultivars, as well as the effect of supplemental glucose (pre and post-storage) on leaf blackening. Glucose, fructose, sucrose and starch concentrations of various Protea species and cultivars held in water were measured at harvest, and again at leaf blackening initiation. All measured carbohydrates declined significantly in 'Carnival', 'Pink Ice' and 'Sheila'. In 'Cardinal' all carbohydrate concentrations decreased significantly, except the sucrose concentration in the inflorescence. 'Susara' and 'Ivy' had very high initial carbohydrate concentrations in the leaves which decreased significantly. The very high initial carbohydrate concentrations in the inflorescence of 'Ivy' declined significantly. 'Brenda' differed from the other cultivars and species in that glucose concentrations increased over time. Carbohydrate concentrations of most of the tested proteas declined significantly from harvest to the initiation of leaf blackening. This highlighted the dependence of the leaves and inflorescence on the carbohydrate reserves, further substantiating the carbohydrate depletion theory. The inflorescences were characterized by high fructose and glucose concentrations and low sucrose concentrations when compared to the leaves. It was hypothesized that glucose pulsing and cold storage at 1°C for three weeks would significantly reduce leaf blackening. 'Brenda', 'Cardinal', 'Carnival', 'Pink Ice', 'Susara ' and 'Sylvia' had significantly less leaf blackening with glucose treatments of 4 and 10%. Leaf blackening of 'Sheila', P. cynaroides and P. grandiceps was not significantly reduced by glucose pulsing. P. magnifica showed a small, but significant, reduction in leaf blackening in response to the 3, 6 and 9% treatments after 10 days only, but despite this, leaf blackening was unacceptably high. 'Pink Ice' harvested at the soft tip stage had less leaf blackening than those harvested open or closed. Toxicity symptoms on the leaves, and in some instances flowers, were observed at higher glucose concentrations (8 and 10%) onP. grandiceps, P. cynaroides, 'Cardinal' and 'Sheila'. All glucose treatments resulted in toxicity symptoms on P. magnifica. A decrease in nonstructural carbohydrates post-harvest apparently occurs in all proteas but it appears that only members of the Ligulatae respond to glucose. Glucose pulsing followed by cold storage at 1°C for three weeks in combination with post-storage glucose vase solutions, significantly reduced leaf blackening of some Protea cultivars. Glucose (1 and 2%), with hypochlorite, significantly delayed leaf blackening in 'Cardinal' and 'Sylvia' after seven days. Leaf blackening of 'Brenda', 'Carnival', 'Pink Ice' and 'Susara' was not significantly reduced by the glucose vase solutions. Other disinfectants, in combination with the sugar treatments, need to be evaluated since the hypochlorite treatment had a dehydrating effect on all the cultivars and resulted in increased leaf blackening. Carbohydrate supplementation of protea flowers with glucose, pre and post-storage, will help meet the post-harvest carbohydrate requirements of certain Protea cultivars and species to an extent. Glucose treatments must be seen in conjunction with maintaining the cold chain and when combined with cold chain maintenance, can extend the storage and vase life. / AFRIKAANSE OPSOMMING: Protea snyblomme word wêreldwyd uitgevoer alhoewel die vaasleeftyd van sommige spesies en kultivars beduidend verkort word deur na-oes loof verbruining. Navorsing het koolhidraatverbruik positief gekorreleer met hierdie probleem. Gevolglik is 'n studie gemaak van die koolhidraatstatus van verskeie spesies en kultivars asook die effek van addisionele glukose (voor en na opberging) op loofverbruining. Glukose, fruktose, sukrose en stysel konsentrasies van verskeie Protea spesies en kultivars wat in water gehou is, is bepaal met oes en weer met die eerste tekens van loofverbruining. Al die gemete koolhidraatkonsentrasies het beduidend afgeneem in 'Carnival', 'Pink Ice' en 'Sheila'. In 'Cardinal' het al die koolhidraatkonsentrasies beduidend afgeneem, behalwe vir die sukrosekonsentrasie in die blom. 'Susara' en 'Ivy' het baie hoë begin koolhidraatkonsentrasies in die blare wat beduidend afneem. Die baie hoë inisiële koolhidraatkonsentrasies in die blom van 'Ivy' neem beduidend af met tyd. 'Brenda' verskil van die ander kultivars en spesies deurdat die glukosekonsentrasies toeneem met tyd. Koolhidraatkonsentrasies van die meeste getoetste proteas neem beduidend af vanaf oes totdat die eerste tekens van loofverbruining verskyn. Dit het die afhanklikheid van die blare en blom op die koolhidraatreserwes beklemtoon en daardeur verder die koolhidraatteorie ondersteun. Die blomme is gekarakteriseer deur hoë fruktose- en glukosekonsentrasies en lae sukrosekonsentrasies wanneer dit met die blare vergelyk is.Die hipotese is gestel dat die voorsiening van glukose, vir 'n aantal ure, gekombineerd met koue opberging by 1°C vir drie weke loofverbruining beduidend sal verminder. 'Brenda', 'Cardinal', 'Carnival', 'Pink lee', 'Susara' en 'Sylvia' het beduidend minder loofverbruining met glukose behandelings tussen 4 en 10%. Loofverbruining van 'Sheila', P. cynaroides en P. grandiceps is nie beduidend verminder deur glukose behandelings nie. P. magnifica het 'n klein, maar beduidende verlaging in loofverbruining getoon met die 3, 6 en 9% behandelings na 10 dae, maar ten spyte hiervan was loofverbruining onaanvaarbaar hoog. 'Pink lee' is geoes by die sagte punt stadium en het minder loofverbruining gehad as blomme wat oop of toe geoes is. Toksisiteitsimptome op die blare, en in sommige gevalle blomme, is waargeneem met hoër glukose konsentrasies (8 en 10%) op P. grandiceps, P. cynaroides, 'Cardinal' en 'Sheila'. Alle glukosebehandelings het toksisiteitsimptome tot gevolg gehad op P. magnifica. 'n Afname in nie-strukturele koolhidrate na oes kom waarskynlik voor in alle proteas maar dit wil voorkom of slegs lede van die Ligulatae positief reageer op glukose. Glukosebehandeling gevolg deur koue opberging by 1°C vir drie weke in kombinasie met na-stoor glukose vaasoplossings het loofverbruining van sommige Protea kultivars beduidend verminder. Glukose (l en 2%), saam met hipoehloriet, het loofverbruining beduidend verminder in 'Cardinal' en 'Sylvia' na sewe dae. Loofverbruining van 'Brenda', 'Carnival', 'Pink lee' en 'Susara' is nie beduidend verminder deur die glukose vaasoplossings nie. Ander ontsmettingsmiddels in kombinasie met die suikerbehandelings moet geëvalueer word aangesien die hipoehlorietbehandeling 'n dehidrerende effek op al die kultivars gehad het en 'n toename in loofverbruining tot gevolg gehad het. Byvoeging van glukose by proteablomme, voor en na opberging, sal tot 'n mate help om in die na-oes koolhidraatbehoeftes van sekere Protea kultivars en spesies te voorsien. Glukosebehandelings moet saam met die beheer van die koueketting gesien word en wanneer gekombineerd met koueketting beheer kan dit opberg en vaasleeftyd verleng.