Thesis (MScAgric) -- Stellenbosch University, 1944. / INLEIDING: Gedurende die afgelope twee dekades, terwyl die wereld die ekonomiese terugslag van die Eerste Wereld-Oorlog in sy volle felheid beleef het, het prysbeheer in verskeie lande meer en meer op die voorgrond getree. Die feit dat na prysbeheer so gretiglik gegryp word, selfs wanneer demokratiese lande in 'n ekonomiese wurggreep worstel, wys op die feit dat prysbeheer beskou ,is as 'n noodhulpmaatreel. Hoe ernstiger die ekonomiese nood, hoe meer algemeen is prysbeheer.

Thesis (MScAgric (Viticulture and Oenology))--University of Stellenbosch, 2011. / Includes bibliography. / ABSTRACT: The grapevine must constantly find a balance between two continually changing environments, the rhizosphere (i.e. soil) and the troposphere (i.e. macroclimate). The adaptations are extremely complex because they encompass complicated and interrelated processes that are not yet fully understood. In terms of water-use behaviour, differences between cultivars have been described in the literature. In this study, the water status and stomatal conductance of four cultivars (Shiraz, Grenache, Pinot noir and Chardonnay) grafted onto R99 were studied. Diurnal cycles of water status and stomatal conductance, from 07:00 to 19:00, were followed for a single day at the end of the 2009 season. The results showed that, at the end of the season, Shiraz was subjected to water stress conditions, losing leaves and showing symptoms of berry shrivelling. The other three cultivars had a much better canopy status and no symptoms of berry shrivelling were observed. Based on the canopy observations and a comparison of the curves of stem water potential (Ψs) and stomatal conductance (gs), it seems that Pinot noir and Chardonnay are closer to the water-use behaviour of Grenache noir, which is known as a “pessimistic” cultivar, than to Shiraz, which is an “optimistic” cultivar. A study of four plots each of Chardonnay/101.14 Mgt and Shiraz/101.14 Mgt was carried out in eight commercial vineyards in the Robertson region in order to investigate the relationship between soil and root morphology, and the influence thereof on canopy development and berry growth. These plots had different soil types. Important soil properties are reported to limit root growth, individually or as a combination of restrictions. It was found that the size of the root system of 101.14 Mgt is defined by soil physical and chemical properties. The roots of 101.14 Mgt under irrigation can grow to a depth of 100 cm or beyond if the soil physical and chemical properties allow it. Because the soil properties define the root system and the water storage/drainage, they greatly influence the plant water status, even under irrigation. In an arid zone like Robertson, irrigation is an important management tool. The balance between canopy growth before véraison and the ability of the root-soil system to maintain that canopy size during the ripening process is crucial in an area with a high evaporative demand. In this regard, not all the soil properties-root system combinations showed satisfactory performance in maintaining the canopy functioning, which affected berry sugar loading and berry volume. In another study that is presented, forty soil profiles were characterised in the Robertson valley. The root systems were considered as a product of the soil properties, and thus the morphology of the root systems was used as a starting point to group soils together. The importance of soil depth has been described well, thus the root systems were first classified according to rooting depth – into shallow and deep root systems. The deep root systems were then subdivided, creating two subgroups of high root density and low root density. The two extreme groups (i.e. shallow roots, and deep roots with high root density) have particularly different soil properties. The soil characteristics found in these extremes are represented up to certain point by families of the South African soil taxonomy, mainly due to the restrictive function of the B horizon. This restrictive function is related to soil properties that are taken into consideration in the South African soil classification and that are important for grapevine root growth, as well as the thickness of the described horizons and the physical and chemical differences between the horizons. Soil properties have an important influence on root morphology. Due to the fundamental role played by the root system in the overall plant functioning, soil properties are of critical importance. In an arid area, the low water pressure in the atmosphere and the high temperature greatly affect the plant water status. The soil-root system combination plays an important role in the ability of the root system to supply the plant with water during times of high evaporative demand. Different cultivars will react differently due to differences in transpiration control. The maintenance of an adequate water status will have an immense influence on canopy development and maintenance, and on normal and steady berry ripening. In this study it was found that not all the soil-root combinations can fulfil this satisfactorily. Thus, the grapevine balance determined by the combination of the soil-root-canopy complex and the influence of management techniques is extremely important for the favouring of a good canopy:root system ratio, a functional canopy throughout the season and a steady berry ripening curve.

Thesis (MScAgric (Viticulture and Oenology))--University of Stellenbosch, 2011. / Includes bibliogaphy. / ENGLISH ABSTRACT: Sauvignon blanc wines have become increasingly popular in South Africa as it is a cultivar that can be easily manipulated in the vineyard and cellar to produce a range of wine styles. These wines are usually given aroma descriptors such as green pepper, grassy and asparagus; while other more tropical aromas include passion fruit and guava. These aromas are thought to be mainly caused by methoxypyrazines and volatile thiols. These compounds are known to be character impacting compounds of Sauvignon blanc and are present in the grapes in the aromatic form (methoxypyrazines) or as non‐aromatic precursors (thiols) that can be released by the yeast during fermentation. Other aroma compounds such as esters, higher alcohols, fatty acids and monoterpenes are compounds that could potentially influence the aroma bouquet of a wine significantly. These aroma compounds exist either as precursors in the grapes (monoterpenes) or arise due to yeast metabolism during fermentation (esters, higher alcohols, fatty acids) and often display fruity, floral and pleasant aromas. In the cellar, winemaking practices can be manipulated to a certain extent to achieve the desired wine style. Winemaking tools such as temperature, skin contact, pressing conditions, oxygen (O2), sulphur dioxide (SO2) and yeast strain are only a few factors influencing the outcome of a wine. In general, South African winemakers maintain a very reductive environment during Sauvignon blanc wine production by using inert gasses, thereby causing the production costs to increase. There is sufficient evidence to support the reductive handling of white wine, however there seems to be a lack of information as to why the must should be treated reductively before fermentation. The over all goal of this study was thus to investigate the effect of different O2 and SO2 additions to Sauvignon blanc must before settling, specifically focussing on the typical aroma compounds often found in these wines. Chapter 2 gives an overview of the oxidation reactions occurring in must (enzymatic oxidation) and wine (chemical oxidation). This chapter also reports the origin of the specific Sauvignon blanc aroma compounds and their reaction to different must and wine treatments with a focus on oxidation. Chapter 3 reports research results focussing on the effect of the different must treatments on the character impacting compounds of Sauvignon blanc wines, specifically the methoxypyrazines and the volatile thiols. The effect of the treatments on the polyphenols and glutathione content in the must and wine was also investigated. Oxidation in the absence of SO2 led to a decrease in glutathione and certain phenolic compounds in the must. In general, volatile thiols were protected against oxidation by SO2, even when O2 was present in the must. Methoxypyrazines concentrations were not significantly influenced by the treatments. Chapter 4 elucidates the effect of the treatments on other yeast and grape derived aroma compounds often found in Sauvignon blanc wines, such as the esters, higher alcohols, fatty acids and monoterpenes. In general, the effect of SO2 seemed to have the greatest influence on the produced aroma compounds. The results reported in this thesis could possibly change the way South African Sauvignon blanc musts are handled in future during the winemaking process. It is clear that O2 and SO2 management in the cellar is of critical importance for the winemaker to produce wines of high quality. Future work is important to fully understand the mechanisms and evolution of important aroma compounds of Sauvignon blanc wines during the winemaking process. / AFRIKAANSE OPSOMMING: Sauvignon blanc wyn aroma word gewoonlik beskryf met terme soos groen rissie, grasagtig en aspersie terwyl ander tropiese aromas soos grenadella en koejawel ook dikwels voorkom. Die manipulasie van Sauvignon blanc in die wingerd en in die kelder tydens wynmaak, gee die wynprodusent die vryheid om ‘n wye reeks wyn style te produseer. Dit maak Sauvignon blanc baie populêr in die Suid‐Afrikaanse wynindustrie. Die bogenoemde aromas word waargeneem in die wyn as gevolg van die teenwoordigheid van sekere aroma komponente genaamd metoksipirasiene en vlugtige tiole. Hierdie komponente lewer ‘n unieke bydrae tot die aroma samestelling van Sauvignon blanc wyne en kom voor in die druiwe in die aromatiese vorm (metoksipirasiene) of as nie‐aromatiese voorlopers (tiole) wat tydens alkoholiese fermentasie deur die gis vrygestel kan word. Komponente soos esters, hoër alkohole, vetsure en monoterpene kan ook ‘n potensiële bydra lewer tot die algehele aroma van Sauvignon blanc wyne en kom voor in die druiwe (monoterpene) of ontstaan as gevolg van gis metabolisme gedurende alkoholiese fermentasie (esters, hoër alkohole, vetsure). Hierdie geur komponente word dikwels beskryf as vrugtig, blomagtig en oor die algemeen aangenaam. Tydens wynmaak kan die wyn tot ‘n mate gemanipuleer word om ‘n spesifieke wynstyl te bekom. Hulpmiddels soos temperatuur, dopkontak, pers omstandighede, suurstof (O2), swawel dioksied (SO2) en gisras is slegs ‘n paar faktore wat die algemene uitkoms van ‘n wyn kan beïnvloed. Oor die algemeen word Sauvignon blanc in Suid‐Afrika baie reduktief behandel tydens wynbereiding. Dit vereis sekere hulpmiddels, soos die gebruik van inerte gas, wat die produksiekoste dikwels verhoog. Navorsing ondersteun die reduktiewe behandeling van wit wyn, maar dit wil voorkom asof daar ‘n tekort aan navorsing is wat die reduktiewe behandeling van die sap voor fermentasie regverdig. Die algemene doel van die studie is dus om die effek van verskillende O2 en SO2 byvoegings tot Sauvignon blanc sap (voor afsak) te ondersoek met die fokus op die tipiese aroma komponente wat in die wyn voorkom. Hoofstuk 2 lewer ‘n algemene oorsig van die tipes oksidasie reaksies wat voorkom in sap (ensiematiese oksidasie) en wyn (chemiese oksidasie). Spesifieke Sauvignon blanc aroma komponente word ook ondersoek in terme van die oorsprong van die komponente asook die reaksie wat plaasvind met verskillende mos en wyn behandelings, met ‘n fokus op oksidasie. In hoofstuk 3 word die effek van die verskillende mos behandelings op tipiese Sauvignon blanc aroma komponente, spesifiek metoksipirasiene en vlugtige tiole, ondersoek. Die effek van die behandelings op die polifenole en glutatioon inhoud in die mos en wyn word ook gerapporteer. Oksidasie van die sap in die afwesigheid van SO2, het ‘n afname in glutatioon en sekere polifenol konsentrasies veroorsaak. Dit wil voorkom asof die produksie van vlugtige tiole oor die algemeen beskerm word teen oksidasie indien daar genoegsame SO2 teenwoordig is. Hierdie effek word ondervind selfs as die sap met suursof versadig word. Die effek van die behandelings op die konsentrasies van metoksipirasiene was nie beduidend nie. Hoofstuk 4 rapporteer die effek van die behandelings op ander aroma komponente soos esters, hoër alkohole, vetsure en monoterpene. Oor die algemeen wil dit voorkom asof die effek van SO2 beduidend was en waarskynlik die grootste invloed op die produksie van hierdie aroma komponente het. Na aanleiding van die resultate bevind in hierdie tesis, is daar ‘n moontlikheid dat die manier waarop Sauvignon blanc wyne geproduseer word in Suid‐Afrika, moontlik kan verander in die toekoms. Vir die wynmaker om hoë kwaliteit Sauvignon blanc wyne te produseer, is O2 en SO2 bestuur in die kelder van kardinale belang. Verdere navorsing moet steeds gedoen word om die meganisme en evolusie van belangrike aroma komponente in Sauvignon blanc wyne tydens die wynmaakproses, ten volle te verstaan.

Thesis (MScAgric (Viticulture and Oenology))--University of Stellenbosch, 2011. / Includes bibliography. / ENGLISH ABSTRACT: Grape and wine phenols consist of flavanols which is the building blocks for tannins. These building blocks are called monomers which consist of catechins, epicatechins, epigallocatechins and epicatechin-gallate. Tannin is important in wine as it contributes to bitterness, mouth feel (astringency) and maturation potential of the wine. Futhermore it has a health benefit as an antioxidant. Anthocyanins are responsible for the colour of red wine. The anthocyanins combine with tannins to form stable polymeric pigments. Due to the importance of tannins and anthocyanins in wine, it is imperitative that different winemaking techniques are used to extract as much of these components as possible and that the analysis is done quickly and accurately. The aim of this study was to evaluate different winemaking techniques and their extraction of tannins and anthocyanins into the wine. Too much tannin extraction can have a negative effect on the sensory quality of the wine. Therefore a second aim was to evaluate the mouth feel properties of a Shiraz wine. A third aim was to compare the two tannin precipitation methods in terms of time efficiency, repeatability and the ease of practice. To investigate the amount of tannin concentration extracted by different winemaking techniques, two cultivars (Cabernet Sauvignon and Shiraz) were used. These treatments included the addition of an enzyme during fermentation [E], cold maceration [CM], post maceration [PM] and the combination of cold and post maceration [CM+PM]. The grapes were harvested in two different climatic areas during the 2008 and 2009 vintages. The two climatic areas were classified according to the Winkler scale as a III (Morgenster) and a IV (Plaisir de Merle). The grapes were harvested at two different ripeness levels in order to evaluate the effect of the different winemaking processes on the extraction of tannins and anthocyanins. One harvest was before (LB) and the other after (HB) the commercial harvest. The results of this study showed significant differences in the phenolic composition of the wines. It was found that the warmer area showed higher tannin concentrations than the cooler area for both cultivars. In the 2008 Cabernet Sauvignon the CM extracted higher concentrations of tannin from the cooler area at both ripeness levels. In the warmer area, CM extracted the highest tannin concentration HB, but the CM+PM extracted the highest tannin concentration from Cabernet Sauvignon at the LB and CM at the HB of the warmer area. In 2009 the PM extracted the highest concentration of tannin at the lower ripeness level, while the E treatment extracted the highest concentration from the warmer area. In the cooler area the CM+PM extracted the highest concentration of tannin at a lower ripeness level, while there were no siginicant differences between the different treatments at the higher ripeness level. The highest anthocyanin concentration was found in the cooler area. The CM treatment was found to have no effect on anthocyanin extraction. Different methods are available to quantify the tannin concentration in wine. Two of the most popular tannin analytical methods are the bovine serum albumin (BSA) and the methyl cellulose precipitable tannin (MCP) methods. The BSA method is a very complex method which uses at least 3 times more reagents than the MCP method. The MCP method only analyzes tannins, while the BSA method analyzes tannins, monomeric pigments (MP), small polymeric pigments (SPP) and large polymeric pigments (LPP). In this study a good correlation was found between the two tannin precipitation methods (R2 – 0.88). There is controversy regarding the variability of these methods. Some scientists found that the two methods show a good correlation with HPLC, while others found that there was no such correlation between the precipitation methods and the HPLC. The MCP method had a practical advantage as it could be performed in half the time required for the BSA method. This has a significant impact in scenarios where a high sample throughput is required although it only measures total tannin. The phenolic composition and mouth feel of the wine was strongly influenced by the climatic area. In the warmer area the effect of tannin concentration on mouth feel was much less than in the cooler area. The wine made of riper grapes, was more grippy, bitter and numbing than the wines made from greener grapes. The E treatment was especially associated with a dry, grippy sensation. / AFRIKAANSE OPSOMMING: Druif en wyn fenole bestaan uit flavanole wat weer die boublokke is van tanniene. Hierdie boublokke, wat bekend staan as monomere, betsaan uit katesjiene, epikatesjiene, epigallokatesjiene an epikatesjien-gallaat. Tanniene is belangrik in wyn aangesien dit bydra tot bitterheid, mondgevoel (vrankheid) asook die verouderingspotensiaal van wyn. As antioksidante hou dit ook gesondheidsvoordele in. Antosianiene dra by tot die kleur van rooiwyn. Antosianiene kombineer met tanniene om meer stabiele polimeriese pigmente te vorm. As gevolg van die belangrikheid van tanniene en antosianiene is dit van uiterse belang dat verskillende wynmaak tegnieke gebruik word om ekstraksie in die wyn te bevoordeel en dat die analitiese metode so vinnig en akkuraat as moontlik gedoen word. Die eerste doel van hierdie studie was om die ekstraksie van tanniene en antosianiene deur middel van verskillende wynmaak tegnieke te evalueer. Te veel tanniene in die wyn kan negatiewe sensoriese kwaliteit tot gevolg het. Daarom is die tweede doel om die sensoriese kwaliteit van Shiraz wyn te evalueer. Die derde doel van hierdie studie was die twee tannien presipitasie metodes met mekaar te vergelyk in terme van die moeilikheidsgraad van die metode, tyd doeltreffendheid en herhaalbaarheid. Verskillende wynmaak tegnieke (ensiem byvoegings [E], koue maserasie [CM], verlengde dopkontak [PM] en ‘n kombinasie van koue maserasie en verlengde dopkontak [CM+PM]) is vergelyk ten opsigte van tannien en antiosianien ekstraksie. In 2008 en 2009 is twee kultivars (Cabernet Sauvignon en Shiraz) in twee verskillende klimatologiese areas gepars. Hierdie areas is geklassifiseer in die Winklerskaal as ‘n IV (Plaisir de Merle) en ‘n III (Morgenster). Om die effek van die verskillende wynmaak tegnieke op die ekstraksie van antosianiene en tanniene te vergelyk, is hierdie twee kultivars by twee verskillende rypheidsgrade geoes. Die eerste oes was net voor kommersiële oes (LB) en die tweede oes het net na kommersiële oes (HB) plaasgevind. Die 2009 Shiraz wyn is organolepties beoordeel om die effek van die verskillende wynmaak tegnieke op die wyn se mondgevoel te vergelyk. Die resultate van hierdie studie toon beduidende verskille in die fenoliese samestelling van die wyne. Dit is gevind dat die warmer area hoër tannien konsentrasies het as die koeler area. In 2008 het die CM+PM die meeste tanniene uit die Cabernet Sauvignon geëkstraheer by LB en die CM by HB in die warmer area. Die CM het in die koeler area meer tanniene geëkstraheer by beide die LB en HB rypheidsgrade. In 2009 het PM die meeste tanniene geëkstraheer by LB terwyl E die meeste tanniene geëkstraheer in die warmer area. In die koeler area het CM+PM die meeste tanniene geëkstraheer, terwyl geen van die behandelings ‘n effek gehad het by HB. Die meeste antosianien konsentrasie was in die koeler area gevind as in die warmer area. In beide 2008 (LB en HB) en 2009 (LB) het CM die meeste antosianiene geëkstraheer, terwyl geen behandeling ‘n effek gehad het by HB. Twee van die mees populêre tannien analitiese metodes is die BSA (bovine serum albumien) en die MCP (metielsellulose presipitasie) metodes. Die BSA metode is ‘n baie meer ingewikkelde metode waarvoor drie keer meer reagense gebruik word as vir die MCP metode. Maar waar die MCP net tanniene ontleed, ontleed die BSA metode tanniene, monomere (MP), klein polimeriese pigmente (SPP) en groot polimeriese pigmente (LPP). Dit help indien daar gekyk wil word na die evolusie van polimeriese pigmente. In hierdie studie is bevind dat daar ‘n redelike korrelasie (R2 – 0.88) tussen die BSA en MCP metode bestaan. Die herhaalbaarheid van die metodes het redelike kontroversie veroorsaak, waar sommige navorsers bevind het dat die BSA metode nie so herhaalbaar is soos eers bevind is nie. Die MCP metode het ’n praktiese voordeel aangesien dit in die helfde van die tyd van die BSA metode uitgevoer kan word. Dit het ‘n groot impak indien ‘n groot hoeveelheid monsters ontleed moet word. Die fenoliese samestelling en mondgevoel word sterk beïnvloed deur die klimatologiese area. In die warmer area was die effek van tannien konsentrasie op mondgevoel kleiner as in die koeler area. Die wyn van ryper druiwe het meer harder, verdowingseffek en bitter nasmaak gehad as by die wyn van groener druiwe. Die ensiem behandeling was meer geassossieerd met droë mond gevoel.

Thesis (MScAgric)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: Irrigation scheduling decisions are based either on the direct measurement of soil water status (SWS) or on physiological measurements like plant water status (PWS). Soil based measurements are quick and easily automated, but the plant response for a particular quantity of soil moisture varies as a complex function of evaporative demand. A plant-based approach measures the plant stress response directly, but is an integration of environmental effects as well. In contrary to soil-based methods, plant based measurements can indicate when to irrigate, but not the quantity. Pre-dawn leaf water potential (ΨPD) is determined mostly by the soil moisture level, and can serve as a measure of static water stress in plants and an index of bulk soil water availability or even as an estimate of soil water potential at the root surface. Therefore it should be possible to establish a link between SWS and PWS, but it is largely unknown how stable the link in a heterogeneous vineyard would be, and how the grapevine vegetative and reproductive response relates to this link. Plant water status plays a large role in determining vigour and yield of the plant. The levels of PWS are influenced by irrigation, but it was mostly affected by the season and vine location in the vineyard. More negative plant water potentials reduced vigour, but had a less pronounced effect on yield, while also reducing overall wine quality. Vigour variability in the vineyard was largely attributed to soil heterogeneity, which seemed to have a strong effect on SWS. SWS measurements were calibrated according to the observed variability, increasing the accuracy of measurements significantly. Soil water content values were used to establish a link between SWS and PWS. This link was determined over time using nine plots, consisting of rain-fed and irrigated regimes, in variable vigour areas. A non-linear relationship was found between ΨPD and percentage extraction of plant available water for rain-fed plots. When irrigation was applied, no correlation could be found. In this study, for Merlot in the Stellenbosch region, PWS differences affected vigour, and to a lesser extent yield, as well as wine quality. More negative plant water potentials reduced vigour more in high vigour areas than in lower vigour areas, which in turn led to unbalanced vegetative: reproductive ratios. This disturbed vine balance may have had a bigger impact on wine quality than PWS levels. Therefore a well-managed and balanced vine is able to withstand more stress, with less detrimental effects. This study also highlights the danger of limiting the assessment of soil and plant water status conditions to point measurements in vineyards with high levels of vigour variability. / AFRIKAANSE OPSOMMING: Besluite rakende die skedulering van besproeiing word gewoonlik gebaseer op die direkte meting van grondwaterstatus (GWS), of op fisiologiese metings soos byvoorbeeld plantwaterstatus (PWS). Grond gebaseerde metings is relatief vinnig en maklik om te outomatiseer, maar die plantrespons vir ʼn spesifieke grondwaterinhoud varieer as ʼn komplekse funksie van dampdruktekorte. ‘n Plantgebaseerde benadering meet die plantstresreaksie direk, maar is ʼn integrasie van omgewingstoestande. In teenstelling met grondgebaseerde metodes, kan plantgebaseerde metodes aandui wanneer om te besproei, maar nie die hoeveelhede wat besproei moet word nie. Voorsonsopkoms blaarwaterpotensiaal (ΨPD) word grootliks deur die grondwaterinhoud bepaal, en kan as ʼn maatstaf van statiese waterspanning in plante en as ʼn indeks van bulk grondwaterbeskikbaarheid dien, of selfs as ʼn benadering van die grondwaterpotensiaal by die worteloppervlak. Dit behoort dus moontlik te wees om ʼn verwantskap te bepaal tussen GWS en PWS, maar dit is grootliks onbekend hoe stabiel hierdie verwantskap sal wees in ʼn heterogene wingerd, asook hoe die wingerdstok se vegetatiewe en reproduktiewe reaksie die verwantskap kan beïnvloed. Plantwaterstatus speel ʼn groot rol in die bepaling van groeikrag en opbrengs in die wingerdstok. Die vlakke van plantwaterstatus word deur besproeiing beïnvloed, maar word skynbaar meesal deur die seisoen en wingerdstok se ligging in die wingerd bepaal. Meer negatiewe plantwaterpotensiaalvlakke het gelei tot laer groeikrag, maar het ʼn minder uitgesproke effek gehad op opbrengs, terwyl dit in die algemeen wynkwaliteit verswak het. Groeikrag variasie in die wingerd kon grootliks aan grond heterogeniteit toegeskryf word, wat skynbaar ʼn sterk invloed op grondwaterstatus gehad het. Grondwaterstatus metings is gekalibreer volgens die variasie wat waargeneem is, wat die akkuraatheid van metings beduidend verhoog het. Grondwaterinhoud waardes is gebruik om ʼn verwantskap aan te dui tussen SWS en PWS. Hierdie verwantskap is oor tyd bepaal vir nege persele, wat bestaan het uit droëland asook besproeide persele, in areas waarvan die groeikrag verskil het. ʼn Nie-liniêre verband is gevind tussen ΨPD en die persentasie onttrekking van plantbeskikbare water vir die droëland persele. Waar besproei was, kon geen verband gevind word nie. In hierdie studie, vir Merlot in die Stellenbosch area, het PWS vlakke groeikrag en tot ʼn mindere mate opbrengs en wynkwaliteit beïnvloed. Meer negatiewe plantwaterpotensiaal vlakke het groeikrag meer beïnvloed in hoër groeikrag areas as in die laer groeikrag areas, wat ook gelei het tot ongebalanseerde vegetatiewe:reproduktiewe verhoudings. Hierdie versteurde balans in die wingerdstokke kon dalk ʼn groter impak op wynkwaliteit gehad het as PWS vlakke. Daar moet dus gepoog word om goed bestuurde en gebalanseerde wingerdstokke te hê, sodat strestoestande beter weerstaan kan word met minder nadelige gevolge. Die studie beklemtoon ook die gevaar verbonde daaraan om die bepaling van grond- en plantwaterstatus te beperk tot puntmetings in wingerde met groot variasie in groeikrag.

Thesis (PhD(Agric) (Agronomy))--University of Stellenbosch, 2009. / ENGLISH ABSTRACT: The use of capacitance water sensors for the scheduling of irrigation for hydroponic tomato and cucumber crops grown in coir was investigated in a series of laboratory and glasshouse experiments in the Free State province of South Africa. Laboratory experiments in a climate controlled chamber were conducted to accurately calibrate ECH2O capacitance sensors, models EC-10 and EC-20, in coir with an improved calibration procedure. Water content predictions by the coir-specific calibration and manufacturer’s calibration equations were compared to actual water content measured from mass loss of the coir sample. The manufacturer’s calibration equation indicated a poor accuracy of prediction, which mostly underestimated the volumetric water content, compared to the near perfect prediction of the coir-specific calibration of individual sensors. A rapid calibration procedure for EC-10 and EC-20 sensors was proposed to reduce the calibration time of the sensors and promote their commercial use for irrigation management in coir. The accuracy of prediction by the rapid calibration procedure for the plant available water content range was high for both EC-10 and EC-20 sensors and allowed for the compensation for variation between sensors. Glasshouse studies aimed to characterise the water retention and ability of coir to supply water to greenhouse tomato and cucumber crops through the continuous monitoring of medium water content in small and large growing bags with the EC-10 and EC-20 capacitance sensors during a drying cycle, compared to well-watered plants. Stages of crop water stress were identified and, based only on the plant’s response to the drying cycle, it was suggested that water depletion can be allowed to the point of mild water stress for both greenhouse tomato and cucumber crops, which can be detected by soil water sensors. In a second series of glasshouse experiments, the identified stages of crop water stress were used to determine and apply depletion levels in coir and compare this irrigation strategy to a well-watered treatment for greenhouse cucumber and tomato plants, with regard to the water balance components, yield and water use efficiency for different bag sizes. Results indicated that irrigation was successfully managed to the pre-determined water depletion levels for cucumber and tomato plants in coir, through the use of in situ calibrated capacitance sensors. For both crops the depletion of water varied between bag sizes, indicating that various bag sizes require different irrigation management strategies. Scheduling to the highest pre-determined by 124 L m-2 in the small and 240 L m-2 in the large bags for cucumbers and 427 L m-2 in the small and 487 L m-2 in the large bags for tomato plants, compared to the well-watered treatments. Yields achieved by the greenhouse tomato plants in the large growing bags and cucumber plants in the small and large bags were maintained or improved when scheduled to the highest depletion level (approximately 60% available water content) compared to the well-watered treatment. The combination of reduced irrigation and improved or maintained yields resulted in improved water use efficiencies (based on irrigation and transpiration) for the highest depletion level compared to the well-watered treatments. In all glasshouse experiments the well-watered treatment resulted in luxury water use by the plants. Finally, a study was conducted in order to compare crop water stress of greenhouse cucumber and tomato plants under luxury water supply and cyclic water deficit conditions. The comparison was based on the transpiration ratio and yield, while the use of capacitance sensors was evaluated for irrigation scheduling in coir for both crops. Transpiration data indicated that cucumber and tomato plants subjected to luxury water supply experience water stress earlier than plants subjected to cyclic water deficit conditions, irrespective of bag size. Results also indicated that irrigation scheduling according to water depletion levels in small bags is not yet recommended for greenhouse tomato and cucumber plants grown in coir, until further research is conducted. Scheduling to water depletion levels in large bags is, however, justified by the improved or maintained yields of the greenhouse cucumber and tomato plants. The estimated depletion levels for large bags beyond which yield are reduced was at 85% for tomatoes and 70% for cucumbers. In conclusion, the results clearly indicated that the use of capacitance sensors in large growing bags improves irrigation management of hydroponic cucumbers and tomatoes in coir by eliminating over-irrigation and improving water use efficiency. More research is needed before a conclusion can be made regarding irrigation scheduling with capacitance sensors in small growing bags. / AFRIKAANSE OPSOMMING: Die gebruik van kapasitansie water sensors vir besproeiingskedulering van tamatie en komkommer plante wat hidroponies in kokosveen gegroei is, is ondersoek in ‘n reeks laboratorium en glashuis eksperimente in die Vrystaat provinsie van Suid Afrika. Laboratorium eksperimente is uitgevoer in ‘n klimaat beheerde kas om ECH2O kapasitansie sensors, modelle EC-10 en EC-20, akkuraat te kalibreer vir kokosveen deur ’n verbeterde kalibrasie prosedure. Waterinhoud voorspellings deur die kokosveen spesifieke kalibrasie en die vervaardiger se kalibrasie vergelykings is vergelyk met die werklike waterinhoud wat gemeet is deur die kokosveen monster se massaverlies te monitor. Akkuraatheid van voorspelling deur die vervaardiger se kalibrasie vergelykings was swak en het meestal die volumetriese waterinhoud onderskat in vergelyking met die byna perfekte voorspelling deur die kokosveen spesifieke kalibrasie van individuele sensors. ’n Vinnige kalibrasie prosedure vir die EC-10 en EC-20 sensors is voorgestel om die kalibrasie tyd te verkort en die kommersiële gebruik van die sensors vir besproeiingsbestuur in kokosveen aan te moedig. Die akkuraatheid van voorspelling deur die vinnige kalibrasie prosedure, binne die grense van plant beskikbare waterinhoud, was hoog vir beide EC-10 en EC-20 sensors, terwyl die prosedure ook voorsiening maak vir variasie tussen sensors. Glashuis studies is uitgevoer om die water retensie en vermoë van kokosveen om water te voorsien aan tamatie en komkommer gewasse in kweekhuise, te karakteriseer. Dit is bereik deur die mediumwaterinhoud van klein en groot plantsakke deurlopend te monitor met behulp van die EC-10 en EC-20 kapasitansie sensors gedurende ’n uitdroging siklus, en dit te vergelyk met ’n waterryke behandeling vir elke gewas waarvolgens die plante agt keer per dag besproei is. Fases van gewas waterstremming is geïdentifiseer en, volgens die reaksie van die plant tot die drogingsiklus, is dit voorgestel dat wateronttrekking toegelaat kan word tot die punt van matige waterstremming wat aangewys kan word deur kapasitansie water sensors vir beide kweekhuis tamatie en komkommer gewasse. In ’n tweede reeks glashuis eksperimente is die geïdentifiseerde fases van gewas waterstremming gebruik om onttrekkingsvlakke vir kokosveen te bepaal en toe te pas as besproeiingskeduleringstrategie vir kweekhuis komkommer en tamatie plante. Toegepaste vlakke is vir elke gewas vergelyk met ’n waterryke behandeling ten opsigte van die waterbalans komponente, opbrengs en watergebruiksdoeltreffendheid in verskillede sakgroottes. Resultate het aangedui dat besproeiing suksesvol bestuur is tot die voorafbepaalde wateronttrekkingsvlakke vir komkommer entamatie plante in kokosveen, deur gebruik te maak van in situ gekalibreerde kapasitansie sensors. Die onttrekking van water deur beide gewasse het verskil tussen klein en groot sakke, wat aangedui het dat verskillende sakgroottes verskillende besproeiingsbestuur strategieë vereis. Skedulering tot die hoogste voorafbepaalde onttrekkingsvlak het, in vergelyking met die waterryke behandelings, besproeiing verminder met 124 L m-2 in die klein en 240 L m-2 in die groot sakke vir komkommers, en 427 L m-2 in die klein en 487 L m-2 in die groot sakke vir tamatie plante. Opbrengste van kweekhuis tamatie plante in die groot plantsakke en komkommer plante in die klein en groot sakke is gehandhaaf of verbeter deur skedulering tot die hoogste onttrekkingsvlak (ongeveer 60% van beskikbare water inhoud), in vergelyking met die waterryke behandeling. Die kombinasie van verminderde besproeiing en verbeterde of gehandhaafde opbrengste het gelei tot verbeterde watergebruiksdoeltreffendheid (besproeiing en transpirasie) vir die hoogste onttrekkingsvlak, in vergelyking met die waterryke behandelings. In al die glashuis eksperimente het die waterryke behandeling gelei tot oorvloedige watergebruik deur plante. ’n Finale studie is uitgevoer om gewas waterstremming van kweekhuis komkommer en tamatie plante wat onderwerp is aan oorvloedige watervoorsiening deur agt keer per dag te besproei en sikliese watertekorttoestande, te vergelyk. Die vergelyking is gebaseer op die transpirasie verhouding en opbrengs, terwyl die gebruik van kapasitansie sensors vir besproeiingskedulering in kokosveen vir beide gewasse geëvalueer is. Transpirasie data het aangedui dat komkommer en tamatie plante wat onderwerp is aan oorvloedige watervoorsiening vroeër waterstremming ervaar as plante wat onderwerp is aan sikliese watertekorttoestande, ongeag van die sakgrootte. Resultate het aangedui dat besproeiingskedulering volgens wateronttrekkingsvlakke vir klein sakke nog nie aanbeveel kan word vir kweekhuis tamatie en komkommer plante alvorens verdere navorsing gedoen is nie. Skedulering tot wateronttrekkingsvlakke vir groot sakke word egter geregverdig deur die verbeterde of gehandhaafde opbrengste van kweekhuis komkommers en tamaties. Die beraamde laagste onttrekkingsvlakke vir groot sakke wat nie opbrengs betekenisvol sal beïnvloed nie is 85% vir tamaties en 70% vir komkommers. Ten slotte dui die resultate duidelik daarop dat die gebruik van kapasitansie sensors in groot plantsakke besproeiingsbestuur van hidroponiese komkommers en tamaties in kokosveen verbeter deur oorbesproeiing uit te skakel en die watergebruiksdoeltreffendheid te verbeter. Meer navorsing is nodig alvorens ’n gevolgtrekking gemaak kan word ten opsigte van besproeiingskedulering met kapasitansie sensors in klein plantsakke.

Thesis (PhD(Agric) (Agronomy))--Stellenbosch University, 2008. / The adoption of the no-till planting method brought about changes to the way the wheat crop is established in the Mediterranean climate of the Western Cape. Row widths have to increase from the normal narrow rows (170-180 mm) to at least 250 mm to allow for sufficient stubble handling. Furthermore, planters are designed to place seed accurately in the soil at uniform depth, which may increase seedling survival rates. The main objective of this study was to determine the influence of the use of wide row widths on yield, the components of yield and grain quality parameters and to revisit planting density recommendations to be used with the notill planting method. On-farm, producer managed trials which included cultivars, row widths and planting density treatments were planted at Riversdale, Swellendam and Caledon in the Southern Cape region and at Moorreesburg and Hopefield in the Swartland during the 2004 to 2006 production seasons. All trials were factorial RCB designs with split-split plot arrangements. Grain yield, grain protein, hectolitre mass (HLM) and the yield components, seedlings m-2, seedling survival (%), number of heads m-2, number of heads plant-1, number of kernels head-1 and thousand kernel mass (TKM) were determined at all sites in 2005 and 2006. Seedling survival rates of 80% were easily achieved in all trials with the exception of Caledon and Swellendam in 2005. The no-till planting method may be efficient to improve on survival rates of 50-70% found with the conventional planting methods. The yield component response that raised the most concern was the clear trend of the reduction in the number of heads m-2 as row widths increased, which was significant in eight out of the nine experiments. The number of heads plant-1 decreased significantly as planting density increased in all experiments. Cultivars differed in the grain quality parameters grain protein (%) and HLM but were influenced minimally by the other treatments. Reductions in grain yield occurred in three out of eight trials in the Southern Cape and in three out of six trials in the Swartland, with reductions of between 6.8% and 33% in some seasons. The risk of yield loss due to wide row widths could not be excluded by this study and therefore the row widths used by producers should remain as narrow as practically possible. Grain yield response to increasing planting density differed between the two regions. No significant yield benefits were found in any of these trials if planting densities were increased above 175 target plants m-2. Planting densities may be reduced to between 70 and 87.5 kg seed ha-1 to achieve this target if the crop is planted in time and seedling survival rates of at least 80% can be achieved.

Thesis (PhD(Agric) (Animal Sciences))--Univsersity of Stellenbosch, 1992. / The anatomy of the muscles of the hindquarters of the ostrich is described and presented graphically for the purpose of the present and future studies in meat science involving the ostrich. In the past, the lack of an accurate anatomical description of this species has led to the incorrect identification of certain muscles by researchers. The process of growth is described for 51 components of the ostrich body, which includes 21 muscles and four organs. Gompertz functions were calculated to formulate growth models for these components. Acceptable goodness-of-fit was obtained for the formulated models regarding the raw skin area, mass of the head, heart, filet and other muscles as well as certain bones. No information on similar models could he found for the description of growth of individual muscles or body parts of other meat animals. However, similar growth models are available for the description of live mass changes over time. Since the general growth curve is sigmoidal it is difficult to fit accurate curves by arithmetical methods. This has led to the development of simpler linear models, which express the growth of a body part relative to the growth of the same entire body. Time based predictions of yield are thus impossible. In fact, many of these allometric equations are simply in a different mathematical form, rather than being totally different equations. Furthermore, the latter equations cannot be used to predict accurate values at the positive and negative extremes since unrealistic values are obtained. Computer programs are presented in the dissertation which can be used without modification on various species, such as fish, poultry, plants and forests, as well as micro organisms in plant pathology, food science and sewage. With the development in computer technology and by means of the suggested programs, it is possible to describe the growth process on a time scale with ease. It is also possible to estimate the mass of any body part at birth (and possibly at conception) as well as at maturity. Evidence is provided that unrealistic asymptotic values are obtained with time based linear and polynomial functions as well as with published non linear allometric models for the description of growth in the ostrich. A goodness-of-fit test for the Gompertz function is presented. It is advisable to carry out this test before fitting alternative models, since the relationship between two variables is only linear on a double log scale when both variables can be described by similar functions on a time scale. A method for the calculation of the standard error at the point where maximum growth occurs is also presented. These methods can successfully be used for any of the above mentioned species. The point at which maximum growth occurred was used to compare the muscles of the hindquarters of the ostrich with that of Merino rams. This showed that these two species differ markedly in this regard. Furthermore, it was proved that certain economically important muscles still gained weight after the optimal skin size (120 dm2) was reached at the age of ten months. It is also shown that the fat content of ostrich meat (filet) varied independently of the abdominal fat content, and that it reached a minimum value of 0.3% at the popular slaughter age of 14 months. Reliable methods for age estimation of a slaughtered bird is presented, based on the growth models. This information can be used to develop a classification system for ostrich meat and other meat animals.

Thesis (MScAgric)--University of Stellenbosch, 1994 / 127 Leaves printed single pages, preliminary pages i-viii and numberd pages 1-118.Includes bibliography,tables and figures. / Date on t.p.: Dec. 1994. / ENGLISH ABSTRACT: Effects of pH,sucrose, boric acid and temperature on in vitro germination of pollen of Protea repens (L.) L. cv. 'Embers' were investigated in hanging-drop culture to establish optimum conditions for germination. Optimum values were found within ranges pH: 5 - 8, sucroseconcentration:0.4 - 0.7 M, boric acid concentration:50 - 500 mg.e-1 , and incubation temperature: 5 - 30°C. Storage temperature and humidity on pollen viability was studied in four Pro tea clones. Pollen was stored at a range of temperatures and relative humidities for up to one year and tested for ability to germinate in vitro. Pollen of P. repens cv. 'Sneyd', P. eximia cv. 'Fiery Duchess' andP. magnifica clone 'T 84 07 OS', stored in liquid nitrogen (-196°C) and in a freezer (-14° to -18°C), retained a germination percentage as high as that of fresh pollen regardless of humidity. The study showed that long-term storage of protea pollen is not feasible at temperatures above O°C. The correlations between the fluorochromatic reaction (FCR) and germinability were found to be low and nonsignificant. Fifteen month old cryopreserved 'Sneyd' pollen was shown to retain its ability to fertilize and set seed equal to that of fresh pollen. 'Sneyd', 'Fiery Duchess' and 'T 84 07 OS' pollen could be repeatedly thawed and frozen in liquid nitrogen before its germinability in vitro decreased. The morphology and size of Protea pollen was studied, using light and scanning electron microscopy. Polymorphic grains were observed in two interspecific hybrids. Very small differences in pollen grain size were recorded between clones/species. The male fertility of 25 interspecific Pro tea hybrids, based on in vitro pollen germinability, was investigated. The majority of hybrids were found to be sufficiently fertile to be used in a breeding programme. Pistil structure and pollen tube pathways were investigated in 'Sneyd' using light and scanning electron microscopy. The pistil had four distinct regions, consisting of the stigma, the vertebra-shaped upper style, the heart-shaped lower style, and the ovary. The pistil had a stylar canal along its entire length and this canal was also the route by which pollen tubes grew to the ovary. Very low numbers of pollen tubes reached the ovary. The breeding system of 'Sneyd' and 'Fiery Duchess' were determined from pollen tube and seed set data, after controlled hand-pollinations. Both clones were found to be fully selfcompatible. Very low percentages autogamous seed set were recorded. Interspecific crosses had a low success rate. An incompatibility reaction probably occurred on the stigma and/or in the upper style regions.The attainment of maximum stigma receptivity of two Protea cultivars was investigated by means of seed set experiments, pollen tube growth observations and measurement of the degree of opening and closing of the stigmatic groove. Both cultivars were found to be protandrous. The maximum stigmatic groove width of both cultivars never exceeded the pollen grain diameter. It was concluded that Protea spp. must be hand-pollinated two to six days after anthesis in order to obtain maximum seed set; while for the observations of pollen tubes in the ovary, inflorescences must not be harvested before seven days after pollination. / AFRIKAANSE OPSOMMING: Ten einde 'n optimale medium vir die in vitro-ontkieming van Protea-stuifmeel te ontwikkel, is die effek van pH, sukrose, boorsuur, en temperatuur op die in vitro-ontkieming van Protea repens (L.) L. cv. 'Embers'-stuifmeel deur middel van die hangdruppel-metode ondersoek. Die volgende reekse van veranderlikes wat getoets is, is as optimaal gevind; pH: 5 - 8, sukrosekonsentrasie: 0.4 - 0.7 M, boorsuurkonsentrasie: 50 - 500 mg.e-1 en inkubasietemperatuur: 5 - 30°C. Die invloed van bergingstemperatuur en humiditeit op stuifmeel-Iewenskragtigheid is in vier Protea-klone ondersoek. Stuifmeel is gestoor by 'n reeks temperature en relatiewe humiditeite vir tot eenjaar, en vir in vitro-ontkiemingsvermoe getoets.' Stuifmeel van P. repens <?v. 'Sneyd', P. eximia cv. 'Fiery Duchess', en P. magnifica kloon'T 84 07 OS', in vloeibare stikstof (-196°C) en in 'n vrieskas (-14° tot - 18°C) geberg, het 'n ontkiemingspersentasie gelykstaande aan die van vars stuifmeel gehandhaaf, ongeag van die humiditeit. Hierdie studie het verder aangetoon dat langtermynberging van Protea-stuifmeel bokant O°C me die moeite werd is me. Die korrelasie tussen die fluorochromatiese reaksie (FCR) en ontkieming was laag en me betekemsvol me. 'Sneyd' -stuifmeel wat vir 15 maande in vloeibare stikstof gestoor is, het die bevrugtings- en saadsetvermoe gelykstaande aan vars stuifmeel behou. 'Sneyd', 'Fiery Duchess' en 'T 84 07 OS'-stuifmeel kon herhaaldelik in vloeibare stikstof gevries en ontdooi word voordat hul ontkiemingsvermoe afgeneem het. Die morfologie en grootte van Proteastuifmeel is deur middel van lig- en skandeerelektronmikroskopie bestudeer. Polimorfiese stuifmeelkorrels is in twee interspesie-hibriede waargeneem. Baie klein verskille in stuifmeelkorrelgroottes het tussen klone/spesies voorgekom. Die manlike vrugbaarheid van 25 Protea-interspesiehibriede, gebaseer op die in vitro-ontkiembaarheid, is ondersoek. Dit is gevind dat die meerderheid hibriede 'n voldoende graad van vrugbaarheid het om in 'n teelprogram te gebruik. Die stamperstruktuur en stuifmeelbuiswee in P. repens is deur middel van lig- en skandeer-elektronmikroskopie ondersoek. Die stamper bestaan uit vier kenmerkende gebiede, naamlik die stempel, die werwelvormige bo-styl, die hartvormige onderstyl, en die vrugbeginsel. Die stamper het 'n stylkanaal regdeur die totale lengte van die stamper, en hierdie kanaal is ook die weg waarvolgens stuifmeelbuise na die vrugbeginsel gegroei het. Min stuifmeelbuise het die vrugbeginsel bereik. Die teelsisteem van 'Sneyd' en 'Fiery Duchess' is deur middel van stuifmeelbuis- en saadsetdata na gekontroleerde handbestuiwings ondersoek. Beide kIone was ten volle selfverenigbaar. Die persentasie outogame saadset was baie laag. Interspesiekruisings het 'n baie lae sukses gehad. Dit is voorgestel dat die onverenigbaarheidsreaksie in die stempel en/of in die bopunt van die styl plaasvind. Die bereiking van maksimum stempelontvanklikheid van twee Protea-cultivars is deur middel van saadseteksperimente, stuifmeelbuisdata en waarnemings van die oop- en toemaak van die stempelgroef ondersoek. Beide cultivars was protandries. Die maksimum stempelgroefwydte het nooit die stuifmeelkorreldeursnee oorskry nie. Dit is afgelei dat Protea-spesies twee tot ses dae na antese handbestuif moet word vir optimale saadset. Vir die waarneming van stuifmeelbuise in die vrugbeginsel, moet bloeiwyses nie voor sewe dae na bestuiwing geoes word nie.

Thesis (MSc)--University of Stellenbosch, 2004. / ENGLISH ABSTRACT: Despite numerous attempts involving a variety of target genes, the successful transgenic manipulation of sucrose accumulation in sugarcane remains elusive. It is becoming increasingly apparent that enhancing sucrose storage in the culm by molecular means may depend on the modification of the activity of a novel gene target. One possible approach to identify target genes playing crucial coarse regulatory roles in sucrose accumulation is to assess gene expression during the developmental transition of the culm from active growth to maturation. This study has resulted in the successful optimisation of a mRNA hybridisation technique to characterise the expression of 90 carbohydrate metabolism-related genes in three developmentally distinct regions of sugarcane culm. A further goal of this work was to extend the limited knowledge of the regulation of sucrose metabolism in sugarcane, as well as to complement existing data from physiological and biochemical studies. Three mRNA populations derived from the different culm regions were assayed and their hybridisation intensities to the immobilised gene sequences statistically evaluated. The relative mRNA transcript abundance of 74 genes from three differing regions of culm maturity was documented. Genes exhibiting high relative expression in the culm included aldolase, hexokinase, cellulase, alcohol dehydrogenase and soluble acid invertase. Several genes (15) were demonstrated to have significantly different expression levels in the culm regions assessed. These included UDP-glucose pyrophosphorylase and UDP-glucose dehydrogenase, which were down-regulated between immature and mature internodes. Conversely, sucrose phosphate synthase, sucrose synthase and neutral invertase exhibited up-regulation in maturing internodal tissue. A variety of sugar transporters were also found to be up-regulated in mature culm, indicating a possible control point of flux into mature stem sink tissues. Combined with knowledge of the levels of key metabolites and metabolic intermediates this gene expression data will contribute to identifying key control points of sucrose accumulation in sugarcane and assist in the identification of gene targets for future manipulation by transgenic approaches. / AFRIKAANSE OPSOMMING: Ondanks verskeie pogings, waartydens verskeie gene geteiken is, is daar nog weinig sukses behaal om sukrose-akkumulering te verhoog. Toenemend wil dit voorkom asof suksesvolle genetiese manipulering van sukroseberging in die stingel van die verandering van ‘n nuwe geen afhanklik sal wees. Een van die moontlike benaderings wat gevolg kan word om potensiële teiken gene wat ‘n belangrike rol in die beheer van sukrose-opberging speel te identifiseer, is om geen uitdrukkingspatrone in die stingel tydens die omskakeling van aktiewe groei tot volwassenheid te karakteriseer. In hierdie studie is ‘n metode gebaseer op die hibridisering van mRNA geoptimiseer en suksesvol aangewend om die uitdrukkingspatrone van 90 verskillende geselekteerde gene, wat vir sleutelensieme in die beheer van koolhidraatmetabolisme kodeer, te bestudeer. Die doel met die ondersoek was om die beperkte kennis oor die regulering van koolhidraatmetabolisme uit te brei en om die bestaande inligting afgelei van fisiologiese en biochemiese-studies aan te vul. Drie verskillende mRNApopulasies, verkry uit verskillende dele van die stingel, is ontleed deur verskillende peilers te gebruik. Die gegewens is statisties ontleed en dit het afleidings oor die verandering in uitdrukking van hierdie gene moontlik gemaak. Die relatiewe konsentrasies van 74 verskillende gene is gedokumenteer. Gene wat sterk uitgedruk word het aldolase, heksokinase, sellulase, alkoholdehidrogenase en ongebonde suurinvertase ingesluit. Die uitdrukkingspatrone van 15 gene het tussen die verkillende weefsels gevarieer. Gene waarvan die uitdrukking tydens die oorgang na volwassenheid verlaag sluit in UDP-glukose pirofosforilase en UDP-glukose dehidrogenase en waarvan die uitdrukking verhoog sukrosefosfaatsintase, sukrosesintase en neutrale invertase in. Die uitdrukking van verskeie suikertransporter gene verhoog tydens volwassewording. Hierdie inligting te same met die huidige kennis oor heersende metabolietvlakke sal bydrae tot die identifisering van geenteikens vir toekomstige genetiese manupulering.