Thesis (MScAgric (Agronomy)--University of Stellenbosch, 2005. / The seven evergreen Disa species are indigenous to South Africa. These orchids grow on mountain ranges subject to winter rainfall and are found on stream banks, around waterfalls and in other damp areas. Although the Disa genus accommodates more than 130 species, by far the most commonly grown is Disa uniflora and hybrids stemming from this species. Disas have great potential as cut flowers and pot plants, but production techniques need to be further investigated since cultivation methods vary greatly between hobbyists. This study evaluated the effect of N-source, shading, root medium temperature, electrical conductivity (EC), irrigation method, foliar feeding at different plant growth stages and substrate on the growth of evergreen Disa hybrids in a controlled environment. Results showed that Disa plants can be classified as being ammonium tolerant. Disa unidiorosa performed best with 40% of the applied N in the NH4 form, while D.kewensis was more tolerant towards a higher level of NH4 and grew best at 60% NH4. Shading levels (56% and 69%) were compared and did not differ regarding the growth of plants. A cooled root medium was found to have a negative effect on root growth and a positive effect on leaf length. High EC levels produced heavier mother plants with a bigger root:shoot ratio and a bigger stem diameter. Biomass accumulation was the best in plants receiving ‘Drip’ irrigation, compared to ‘Ebb-and-Flood’ irrigation treatments. Plants in the vegetative reproducing stage were more susceptible to leaf abscission and new leaves formed at a low rate compared to small- and potential flowering plants. Where foliar feeding is concerned plants seemed to benefit more by the presence of NH4NO3 than urea. There were no significant differences in root development between substrates in the ‘hardening-off’ phase. ‘Hydroton’ (clay pebbles) was not suitable as substrate for the cultivation of Disa plants. The growth and flowering properties of plants were optimal with sphagnum moss and peat but were negatively affected when the pH of acid peat:sand mixtures were increased. More research is needed before Disas can be cultivated on a commercial scale, while the effect of the treatments on flowering properties has to be investigated.

Thesis (MScAgric) -- University of Stellenbosch, 2003. / ENGLISH ABSTRACT: Cowpeas are produced under low and irregular rainfall in most of arid and semi-arid areas of sub-Saharan Africa. Growth and yield are therefore reduced due to the occurrence of water stress during the growing season. Knowledge of the responses and adaptive mechanisms of cowpeas to water stress may help to improve the management practices for these areas. Therefore, three glasshouse experiments were conducted at Welgevallen Experimental Farm of the University of Stellenbosch to test the responses of two cowpea cultivars to water stress. In the first experiment, physiological responses were used to identify those physiological parameters, which can be used to distinguish between drought tolerant and susceptible cowpea cultivars. In the second experiment, some of the identified physiological parameters together with some morphological growth responses, yield and grain protein content of the same two cowpea cultivars were used to identify which is the more tolerant cultivar. Tn the third experiment, the hypothesis that increased phosphorus supply may improve the tolerance of cowpea plants to water stress and their ability of recover from the stress was tested. The results showed that water stress affected water relations, morphological growth parameters, yield and grain protein content, but increasing P supply reduced the effect of water stress and promoted more rapid recovery after re-watering. Water relations were affected by water stress because it reduced relative water content, which resulted in reduced water potential and increased leaf diffusive resistance and proline accumulation. Morphological growth responses and yields were affected because water stress reduced the leaf area, which resulted in reduced biomass production and seed yield. Lower leaf area under water stress was the result of the reduced number of leaves and leaf expansion rate, but the number of leaves was the most important parameter. Reduced seed yield was due to reduced number of pods. The responses of the two cultivars tested were different. AB Wit, which performed better under well-watered conditions was more affected by water stress due to its larger leaf area that resulted in excessive water loss by transpiration. ACH14 was more drought tolerant than AB Wit due to a combination of a more rapid stomatal closure and proline accumulation, which induced osmotic adjustment, and which in tum helped to maintain higher water potentials. The increased P supply reduced the effect of the water stress. High-P level plants showed higher root growth, which resulted in more water uptake and larger leaf area during the water stress period, and after re-watering these plants recovered more rapidly. The more rapid recovery from stress was the result of enhanced root growth and leaf expansion rate and most probably due to increased water uptake. High-P level plants also showed more rapid leaf appearance and plant growth at earlier stages compared to the low-P level plants. / AFRIKAANSE OPSOMMING: Akkerbone word onder toestande van lae en wisselvallige reenval in baie ariede en semi-ariede gebiede van Afrika verbou. In hierdie gebiede word groei en produksie dikwels beperk deur water tekorte gedurende die groei seisoen. Kennis van reaksies en aanpassingsmeganismes van akkerbone teenoor water tekorte mag dus help om produksietegnieke in bogenoemde gebiede te verbeter. Om hierdie rede is drie glashuiseksperimente onder gekontroleerde toestande op die Welgevallen Proefplaas van die Universiteit van Stellenbosch uitgevoer. In die eerste eksperiment is fisiologiese reaksies van twee cultivars gebruik om eienskappe te identifiseer wat gebruik kan word om tussen droogteweerstandbiedende en droogte gevoelige cultivars te onderskei. In die tweede eksperiment is sommige van die geidentifiseerde eienskappe asook morfologiese groei, opbrengs en kwaliteitsreaksies van dieselfde twee cultivars gebruik om die meer droogte weerstandbiedende cultivar te identifiseer. In die derde eksperiment is die hipotese dat P-bemesting die droogteweerstandbiedendheid teen en herstelvermoe na droogte kan verbeter, getoets. Die resultate toon dat water tekorte beide plantwaterverhoudings, morfologiese eienskappe asook opbrengs en proteieninhoud beinvloed, maar dat hoe P-peile die invloed van water tekorte verminder en herstelverrnoe na die droogte verbeter. Plant-waterverhoudings is bemvloed omdat water tekorte relatiewe waterinhoud van plante verlaag wat aanleiding gee tot verlaagde plantwaterpotensiale, verhoogde huidmondjie weerstand en 'n toename in prolien inhoud. Morfologiese eienskappe en opbrengs is benadeel weens 'n veri aging in blaaroppervlakte wat fotosintetiese vermoe en gevolglik ook biomassaproduksie en saad opbrengs benadeel. Verlaagde blaaroppervlakte tydens water tekorte was hoofsaaklik die gevolg van 'n vermindering in aantal blare, terwyl verlaagde saadopbrengs grootliks die resultaat van 'n vermindering in aantal peule was. Die cultivar AB Wit wat die hoogste opbrengs onder gunstige groeitoestande gelewer het, is die meeste bemvloed deur water tekorte omdat die welige blaargroei van hierdie cultivar, luukse waterverbruik en groter transpirasie verliese veroorsaak het. Die cultivar ACH 14 daarteenoor het waterverliese beperk deurdat die huidmondjies vinniger gesluit het en verhoogde prolien-inhoude, osmotiese aanpassings veroorsaak het. Dit het gehelp om waterpotensiale instand te hou. Hierdie cultivar was gevolglik meer droogte weerstandbiedend as AB Wit. Hoe vlakke van P-bemesting het die effek van water tekorte verminder weens verbeterde wortelgroei. Dit het wateropname gedurende en na die peri ode van water stremming verbeter sodat plante vinniger herstel het na die droe periode. Plante wat by hoe P-peile gegroei is het ook 'n verhoogde blaarverskyningstempo en 'n toename in groei tydens die vroee ontwikkelingstadiums getoon.

Thesis (MScAgric)--Stellenbosch University, 2004. / ENGLISH ABSTRACT: The effect of shoot heterogeneity on vegetative and reproductive growth parameters, vine physiology and grape composition was investigated in a ShirazlRichter 99 vineyard. Comparisons between underdeveloped (typically shorter and less ripened at véraison) and normally developed shoots in both shaded (non-manipulated) and well-exposed (manipulated) canopies were made. Compared to underdeveloped shoots, normal shoots had a larger total leaf area, due to the higher occurrence of secondary shoots as well as larger leaves on primary and secondary shoots. Since the photosynthetic activity of the leaves from normal shoots was higher than those from underdeveloped shoots, higher levels of carbohydrates were produced and stored in the former. Starch was more evenly distributed over the length of the whole shoot in normally developed shoots compared to underdeveloped shoots. Normally developed shoots were longer and thicker in diameter than underdeveloped shoots. The larger clusters of the normally developed shoots are evidence of their more favourable total leaf area per gram berry mass. Berries from the normally developed shoots were smaller at five weeks after véraison than those from underdeveloped shoots, displaying a higher skin to pulp ratio and therefore higher anthocyanin and total phenolic extraction potential for winemaking. The smaller clusters and fewer berries per cluster found for the underdeveloped shoots indicate an imbalance between vegetative and reproductive growth initiated during the vegetative growth phase and continued during the ripening period. The peculiar absence of statistically significant differences in grape composition between normally and underdeveloped shoots indicates that assimilates needed for berry ripening of the latter originated in organs other than the leaves [e.g. from adjacent normal shoots and the rest of the permanent structure of the vine (cordon, trunk, roots)]. The larger differences in berry size that occurred between shoot types in the shaded compared to the well-exposed canopies may be evidence for this. The photosynthetic activity of shoots was lower in shaded than in exposed canopies. The total carbohydrate production of the normal shoots in shaded canopies seemed insufficient to supply in the ripening needs of the shoot itself, their own clusters as well as the ripening of stem tissue and clusters of the underdeveloped shoots in the canopy. This is illustrated by the lower levels of starch that accumulated in the normal shoots from shaded compared to that of exposed canopies. Vine shoot heterogeneity clearly leads to visible and physiological imbalances that would impact negatively on grape and wine quality as well as production costs and should therefore be avoided on any terroir. / AFRIKAANSE OPSOMMING: In hierdie studie is die effek van heterogene lootontwikkeling in die lower van 'n ShirazlRichter 99 wingerd ondersoek. Vergelykings is getref tussen normale en onderontwikkelde lote in beskadude en blootgestelde lowers ten opsigte van hul vegetatiewe en reproduktiewe groeieienskappe, fisiologiese aktiwiteit en druifsamestelling en -gehalte. 'n Groter totale blaaroppervlak het by die normaalontwikkelde lote voorgekom as gevolg van die groter aantal sekondêre lote en groter primêre en sekondêre blare. Aangesien die blare van die normaal ontwikkelde lote fotosinteties meer aktief was as dié van onderontwikkelde lote, het die eersgenoemde lote groter hoeveelhede koolhidrate geproduseer en gestoor. Styselopberging het meer eweredig oor die lengte van die normale lote plaasgevind. Laasgenoemde lote was ook heelwat langer en dikker in deursnee as die onderontwikkelde lote. Die gunstiger totale blaaroppervlak per korrelmassa verhouding van die normale lote is duidelik weerspieêl in die groter trosse, terwyl die kleiner korrels (en dus die groter dop:pulp verhouding) op 'n groter potensiaal vir kleur- en fenolekstraksie tydens die wynbereidingsproses dui. Die kleiner trosse en kleiner aantal korrels per tros wat by die onderontwikkelde lote gevind is, dui op 'n wanbalans tussen die vegetatiewe en reproduktiewe groei van die loot wat tydens die vegetatiewe groeifase van die wingerdstok geïnisieer is en tydens die rypwordingsperiode voortgesit is. Die vreemde afwesigheid van enige statisties betekenisvolle verskille in druifsamestelling tussen die normale en onderontwikkelde lote dui daarop dat die verbindings wat vir die rypmaking van trosse op onderontwikkelde lote aangewend is, waarskynlik van ander wingerdorgane (bv. naasliggende lote, kordonarms, stam, wortels) as die spesifieke loot se blare afkomstig was. Die waarneming dat die korrelgroottes van normale en onderontwikkelde lote meer van mekaar verskil het in die beskadude as blootgestelde lowers, kan moontlik as bewys hiervoor dien. Die fotosintetiese aktiwiteit van beide loottipes was laer in die skaduryke lowers. Die koolhidrate wat deur normaalontwikkelde lote in skadu-Iowers geproduseer is, was oênskynlik onvoldoende vir die rypmaking van die loot self, die spesifieke loot se trosse, asook die trosse en lootweefsel van naasliggende onderontwikkelde lote. Hierdie bewerings word gerugsteun deur die laer vlakke van styselakkumulasie wat by die normale lote in beskadude lowers gevind is. Aangesien heterogene lootontwikkeling en -groei duidelike sigbare en fisiologiese wanbalanse in die wingerdstok tot gevolg het wat negatief op druifsamestelling, wyngehalte en produksiekoste inwerk, behoort dit in kommersiêle wingerde vermy te word.

Thesis (MScAgric)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: The potato can be considered as one of the most important food crops in many African countries. The potential of this crop is reflected in the large increase in area of production where Africa showed the highest rate of growth within the developing world over the past twenty years. The multiplication rate of potatoes is very low compared to other crops. Therefore it is essential to investigate methods of increasing the number of minitubers produced from disease free in-vitro plantlets. There is a number of potato propagation procedures that are currently being used worldwide to multiply seed potatoes. As aeroponics is still a relatively new technique that has not been researched extensively for the production of minitubers, a study relating to the production practises including the nutritional requirements of potato minitubers produced in a aeroponic system were undertaken. Potato plantlets, cv BP1, were grown aeroponically at two different densities (20 and 30 plants/m2) and four harvest intervals (7, 10, 14 and 18 days). The interaction between harvesting intervals and plant densities did not influence plant growth, minituber quality or yield. Best results were realised when harvesting every 7 days with a higher total tuber number over the growing period. Harvest interval also influenced the phosphorus and copper concentration in minitubers. To study the effect of Calcium (Ca) application rate, potato plantlets of cultivars Up-to-date, Mnandi, Buffelspoort and BP1 were grown at four different Ca levels (8.40, 6.75, 5.10 and 3.45 meq/L). The interaction between Ca application levels and cultivars significantly influenced the percentage stolon branching. BP1 had more stolons at the lowest Ca application level and Buffelspoort had more stolons at the full Ca application levels. However, low Ca treatments produced the highest yield. The minituber number and weight harvested were three times more for Mnandi. An aeroponic study on the irrigation frequency (20, 30, 40 and 50 minutes interval) was conducted on four potato cultivars (Up-to-date, Mnandi, Buffelsoort and BP1). Significant differences were noted in the interaction between irrigation frequencies and cultivars for the percentage tuberised plants and stolon and tuber dry mass. When irrigated every 40 minutes, 48% of the Buffelspoort plants produced tubers. Plant height was also significantly affected by the interaction between irrigation frequencies and potato cultivars, with Mnandi producing taller plants when irrigated every 30 minutes. Total tuber number and tuber fresh and dry weight was higher at the irrigation frequency of 20 minutes. The interaction between irrigation frequencies and cultivars on the response to macro and trace elements was not significant for sodium and iron but was for phosphorus, potassium, calcium, zinc and aluminium. A field study was conducted in a greenhouse where potato seed of BP1 obtained from the first trial were graded into different sizes (Small: >20, medium: 20–40 and large: > 40 mm of diameter) and stored at 3 different temperatures (3, 16 and 25oC) for 2 supplementary months before being planted. Sprouting capacity was mostly influenced by temperature regardless of other factors applied to potato seed minitubers such as harvest intervals and sizes. The higher storage temperature of 25oC resulted in tubers with a higher number of sprouts, longer sprouts and with a higher sprouting capacity. / AFRIKAANSE OPSOMMING: Aartappels is een van die belangrikste voedselgewasse in baie lande in Afrika. Die potensiaal van die gewas word gereflekteer in die groot toename in produksie areas, met Afrika wat die vinnigste van al die ontwikkelende lande gegroei het die laaste 20. In vergelyking met ander gewasse is die tempo van planvermeerdering by aartappels baie stadig. Dit is dus essensieel om metodes te ondersoek wat sal help om die aantal miniknolle wat per in vitro plantjie verkry kan word te verhoog. Daar is heelparty plant vermeerderings prosedures wat tans wêreldwyd gebruik word om saad aartappels te vermeerder. Aangesien aeroponika nog steeds ʼn relatiewe nuwe tegniek is wat nog nie ekstensief ondersoek is vir die verbouing van miniknolle nie, is ‘n studie geloods om te kyk na die produksie praktyke, wat insluit die voedingsbehoeftes van aartappel miniknolle in ʼn aeroponika sisteem. Aartappel plantjies, kultivar, BP1, is aeroponies verbou by twee plant digthede (20 en 30 plante/m2) en vier oesintervalle (7, 10, 14 en 18 dae). Die interaksie tussen oesintervalle en plantdigtheid het geen effek gehad op plant groei, miniknol kwaliteit of opbrengs nie. Die beste resultate is verkry waar die knolle elke 7 dae geoes is met ‘n hoër totale aantal knolle oor die groeiseisoen. Die oesinterval het ook ‘n effek gehad op die fosfaat en koper konsentrasie van die miniknolle. Om die effek van die Kalsium (Ca) toedieningspeil te ondersoek is aartappel plantjies; kultivars Up-to-date, Mnandi, Buffelspoort en BP1 gekweek by vier verskillende Ca peile (8.40, 6.75, 5.10 en 3.45 meq/L). Die interaksie tussen Ca toedienings peile en kultivars het ‘n beduidende effek gehad op die persentasie stolon vertakking. BP1 het meer stolons gehad by die laagste Ca toedieningspeil en Buffelspoort het meer stolons gehad by die volle Ca toedieningspeil. Die hoogste opbrengste is egter waargeneem by die laagste Ca toedieningspeil. Die aantal miniknolle en oes massa was drie keer meer vir Mnandi. ‘n Aeroponiese studie op die besproeiingsfrekwensie (20, 30, 40 en 50 minuut intervalle) is gedoen met vier aartappel kultivars (Up-to-date, Mnandi, Buffelsoort en BP1). Beduidende verskille is opgemerk in die interaksie tussen besproeiings frekwensie en kultivars vir die persentasie plante met knolle en stolon en knol droë massa. Met besproeiings elke 40 minute het 48% van die Buffelspoort plante knolle produseer. Plant hoogte is ook beduidend beïnvloed deur die interaksie tussen besproeiingsfrekwensie en aartappel kultivar met Mnandi plante wat hoër was wanneer dit elke 30 minute besproei is. Die totale aantal knolle en knol vars- en droë massa was hoër wanneer daar elke 20 minute besproei is. Die interaksie tussen besproeiings frekwensie en kultivars op die makro- en mikro element inhoud van die knolle was nie beduidend vir natrium en yster nie, maar wel vir fosfaat, kalium, kalsium, sink en aluminium. ‘n Potproef is gedoen in ‘n kweekhuis waar aartappel saad van BP1 verkry vanaf die eerste proef nadat knolle verdeel is in verskillende grootte klasse (klein: < 20mm, medium: 20-40mm en groot: >40mm) en gestoor is by drie verskillende temperature (3, 16 en 25oC) vir 2 addisionele maande voor plant. Spruit ontwikkelings kapasiteit was meestal beïnvloed deur temperatuur ten spyte van ander behandelings soos oes intervalle en knol grootte. Die hoër bergings temperatuur 25oC het aanleiding gegee tot knolle met ‘n hoër aantal spruite, langer spruite en ‘n hoër spruit ontwikkelings kapasiteit.

Thesis (MScAgric)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: Three different pasture mixtures were established under irrigation at the Elsenburg research farm with the aim of devising nitrogen (N) management strategies for pastures in the Winelands sub-region of South Africa. The pasture mixtures were as follows: i) a mixed grass pasture consisting of perennial ryegrass (Lolium perenne), tall fescue (Festuca arundinaceae) and cocksfoot (Dactylis glomerata); ii) a grass-clover pasture consisting of perennial ryegrass, tall fescue, cocksfoot and red and white clover (Trifolium pratense and Trifolium repens); and iii) a grass-lucerne pasture consisting of perennial ryegrass, tall fescue and lucerne (Medicago sativa). The effect of fertiliser N on selected nutritive characteristics was also evaluated. The grass-legume pastures were subjected to two management strategies: the once-off application of N and the consecutive application of N over the autumn-early spring period. The reaction of the mixed grass pasture to applied N was mostly characterised by an interaction between the season of N application and N application rate. The productivity of the pasture in terms of the primary dry matter production (PDMP) and the total dry matter production (TDMP) was highest in spring and summer with the application of 60 – 80 kg N ha-1, and decreased in autumn and winter. There was a strong response of the winter residual dry matter production (RDMP) to N, which indicated that not all applied N was utilised during the first regrowth cycle, which might present a risk of nitrate being leached below the root zone. The botanical composition of the mixed grass pasture was determined by season of N application, and not N application rate. The tall fescue content was low over all seasons, presumably due to poor establishment and strong competition from accompanying species. In the cooler months perennial ryegrass and tall fescue was the dominant species, while in the warmer months cocksfoot was the main species. Nitrogen application also had a significant effect on the quality of the pasture, most notably the crude protein (CP) content. The response of the CP content was characterised by a strong interaction between season of N application and N application rate. Crude protein levels in excess of 22 % were recorded in autumn and winter with the application of 40 – 80 kg N ha-1. Other characteristics remained within the expected range. The response of the grass-clover and grass-lucerne pastures in terms of productivity and nutritive characteristics were mainly determined by the season of N application, and not N application rate. Productivity tended to be highest in autumn and early spring for both the once-off and the consecutive N application strategies, emphasizing the effect of temperature on pasture growth. The effect of season of N application and the N application rate on the botanical composition of the respective pastures were inconsistent over the two years of the study. The clover content tended to decrease in response to increasing rates of N, while the grass fraction was stimulated. Lucerne productivity decreased from autumn through winter and reached minimum levels in early spring, and was unaffected by fertiliser N rate. The legume component in both the grass-clover and grass-lucerne pastures remained above recommended levels of 20 – 40 % for optimum animal production, even when N was applied consecutively. The nutritive characteristics measured (dry matter (DM) content, CP, in vitro organic matter digestibility (IVOMD)) remained within the expected range, except the total CP content which was very high in the first year (> 30 %), although N application rate did not have a significant effect. Based on these findings, preliminary recommendations for N fertilisation (on low carbon soils) for a mixed grass pasture is 40 kg N ha-1 during autumn and winter and 60 kg N ha-1 in spring and summer. Based on the poor response of the grass-legume pastures to applied N it is doubtful whether fertilisation will lead to an economical advantage, but low rates of approximately 40 kg N ha-1 could be beneficial in a grass-clover pasture during autumn and late winter/early spring based on the relatively strong response of PDMP to N during this period. / AFRIKAANSE OPSOMMING: Drie verskillende weidingsmengsels is onder besproeiing te Elsenburg proefplaas gevestig met die doel om stikstof (N) bestuurstrategieë te ontwikkel vir aangeplante weidings in die Wynland distrik van die Wes-Kaap van Suid Afrika. Die weidingsmengsels was as volg: i) ‘n gemengde gras weiding bestaande uit meerjarige raaigras (Lolium perenne), langswenkgras (Festuca arundinaceae) en kropaargras (Dactylis glomerata), ii) ‘n gras-klawer weiding bestaande uit meerjarige raaigras, kropaargras, langswenkgras, wit - en rooi klawer (Trifolium pratense en Trifolium repens), en iii) ‘n gras-lusern weiding bestaande uit meerjarige raaigras, langswenkgras en lusern (Medicago sativa). Die effek van stikstof bemesting op sekere kwaliteitsaspekte van die onderskeie weidings was ook geëvalueer. Die gras-peulplant weidings was onderworpe aan twee bestuurstrategieë, naamlik die eenmalige toediening van N en die agtereenvolgende toediening van N bemesting tydens die herfs – lente periode. Die reaksie van die gemengde gras weiding op N bemesting was hoofsaaklik gekenmerk deur ‘n interaksie tussen die N bemestingspeil en die seisoen van N toediening. Die produktiwiteit van die weidings i.t.v. die primêre droeëmateriaal produksie (PDMP) en die totale droeëmateriaal produksie (TDMP) was die hoogste in die lente en somer met die toediening van 60 – 80 kg N ha-1 en het in herfs en winter afgeneem. Daar was n sterk respons van die winter residuele droeëmateriaal produksie (RDMP) teenoor N, wat aandui dat nie alle toegediende N tydens die eerste hergroei periode benut was nie en dus ‘n moontlike risiko van loging inhou. Die botaniese samestelling van die gemengde gras weiding was deur die seisoen van N toediening bepaal, en nie die N bemestingspeil nie. Die langswenkgras inhoud was baie laag in alle seisoene, vermoedelik a.g.v. swak vestiging en sterk kompetisie van gepaardgaande spesies in die mengsel. Tydens die koeler seisoene van die jaar was meerjarige raaigras en langswenkgras die dominerende spesies, terwyl kropaargras tydens die warmer maande gedomineer het. Stikstof toediening het ook ‘n betekenisvolle effek op die kwaliteit van die weiding gehad, veral die ru-proteien (RP) inhoud. Die respons van RP was weereens gekenmerk deur ‘n betekenisvolle interaksie tussen die seisoen van N toediening en die N peil. Ru- proteien vlakke hoër as 22% was tydens herfs en winter waargeneem met die toedieningspyle van 40 – 80 kg N ha-1. Ander kwaliteits- eienskappe het binne normale perke gebly. Die respons van die gras-klawer en gras-lusern weidings in terme van produktiwiteit en kwaliteitseienskappe was hoofsaaklik deur die seisoen van N toediening bepaal, en nie deur die N bemestingspeil nie. Die produktiwiteit was die hoogste tydens herfs en vroeë lente vir beide die eenmalige en die herhaalde N toedieningsstrategieë. Hierdie bevindinge beklemtoon die belangrike effek van temperatuur op die groei en produksie van weidingsgewasse. Die effek van seisoen van N toediening en N peil op die botaniese samestelling van die gras-peulgewas weidings was inkonsekwent oor die twee jare van die studie. Die klawer-fraksie was geneig om af te neem soos wat die N peil toegeneem het, terwyl die gras-fraksie toegeneem het. Die lusern-inhoud het van herfs tot lente afgeneem en was ongeaffekteer deur die N peil. Die peulgewas-inhoud van beide weidingsmengsels was deurentyd hoër as die voorgeskrewe minimum vlak van 20 – 40%, selfs met opeenvolgende N-toediening. Die kwaliteitseienskappe gemeet in die studie (droeëmateriaal (DM) inhoud, RP en in vitro organiese materiaal verteerbaarheid (IVOMV)) het binne normale perke gebly, behalwe die totale ru-proteien (TRP) inhoud wat baie hoog was tydens die eerste jaar (>30%), alhoewel dit nie deur die N peil beinvloed was nie. Aan die lig van bogenoemde bevindinge is die voorlopige aanbeveling vir N- bemesting (op lae koolstof gronde) van ‘n gemengde grasweiding 40 kg N ha-1 tydens die herfs en winter en 60 kg N ha-1 tydens lente en somer. Gebasseer op die swak respons van die gras-peulgewas weidings op toegediende N, is dit twyfelagtig of N toediening enige ekonomiese voordeel vir die boer sal inhou. Gebaseer op die relatiewe sterk respons van die gras-klawer PDMP op toegediende N tydens herfs en laat winter/vroeë lente kan dit moontlik voordelig wees om lae N-vlakke van ongeveer 40 kg ha-1 tydens hierdie seisoene toe te dien.

Thesis (MscAgric (Animal Sciences))--University of Stellenbosch, 2006. / Six non-lactating, ruminally cannulated Holstein cows were used in a three part study to determine the effect of biotin supplementation to dairy cows on forage fermentation characteristics. Cows were randomly assigned to two groups in a 2 x 3 change-over experiment. All cows received oat hay ad libitum and one of two concentrate feeds, fed twice daily at 2 kg per feeding as a top dressing. The concentrates were identical in composition, except for a premix that was included to provide either 0 or 40 mg supplemental biotin/cow per day when the concentrate was fed at a rate of 4 kg/cow. Cows received the respective treatments for 28 days before being changed over to the other treatment. All cows therefore received both treatments. The first 21 days in each period were used for adaptation, while the last 7 days of the period were used for an in sacco trial, as well as for the collection of rumen liquor for two in vitro studies. The in vitro studies were a gas production trial and an in vitro digestibility trial. Forages differing in neutral detergent fibre (NDF) content were used as substrates in the study. Lucerne hay (440 g NDF/kg DM), oat hay (680 g NDF/kg DM), and wheat straw (798 g NDF/kg DM) were chosen to represent high, medium and low quality forages. In the gas production study, samples (0.5 g) of the three forages were incubated at 39ºC in buffered rumen liquor (obtained from cows in the different treatments) in glass vials. Pressure readings were taken after 12, 18, 24, 30 and 48 hours incubation using a digital pressure gauge fitted with a 21 gauge needle. Pressure readings were converted to gas volumes with the aid of a predetermined regression equation. In the in vitro digestibility trial, forage samples (0.25 g) were weighed into Ankom F57 filter bags and incubated at 39ºC in an Ankom Daisy II incubator in buffered rumen liquor. Three bags of each substrate were removed from the incubation jars after 18, 24 and 30 h incubation. Bag residues were analyzed for dry matter, organic matter and NDF. In the in sacco degradability trial, forage samples (5 g) were weighed into 100 x 200 mm Ankom Forage Bags and inserted into the rumina of the respective cow simultaneously. One bag per substrate was removed from each cow at after 4, 8, 18, 24, 30 and 48 h incubation, while two bags per substrate were removed after 72 and 96 h to ensure enough residue for subsequent chemical analysis. Samples of rumen liquor were taken at each of the mentioned incubation times for VFA analysis, while rumen pH was also measured at these times. All the data collected were subjected to a one-way ANOVA, least square means were determined and significance was declared at P<0.05. Biotin supplementation increased the rate of gas production (0-12 h) of all three substrates, as well as cumulative gas production at 48 h. No treatment effects were observed in the in vitro digestion study. Biotin supplementation increased the rate of in sacco NDF disappearance and calculated effective NDF degradability in oat hay and wheat straw, but not in lucerne hay. The rumen pH curve appeared higher for the biotin treatment than for the control and the value at the 72 h sampling time was significantly higher for the biotin treatment than for the control treatment (6.13 vs 5.94). Rumen pH tended to be higher (P<0.10) at 18 h (6.44 vs 6.23), 48 h (6.13 vs 6.00) and 96 h (6.14 vs 6.04). There was also a tendency (P<0.10) for the mean pH over the total 96 h period to be higher for the biotin treatment than for the control (6.09 vs 5.97), while the maximum and minimum pH values did not differ between treatments. Molar proportions of volatile fatty acids did not differ between treatments and the acetic acid proportion was relatively high (acetic:propionic = 74:15), which was probably because the cows were not on a very high concentrate diet. It was concluded that biotin supplementation to dairy cows may improve fermentation rates and NDF digestibility of certain forages.

Thesis (MScAgric)--University of Stellenbosch, 2007. / ENGLISH ABSTRACT: AN ASSESSMENT OF PERFORMANCE EVALUATION PROCEDURES OF DORPER RAMS UNDER EXTENSIVE MANAGEMENT CONDITIONS. During 1988 to 2000, Dorper rams (n=2565) maintained on the Kalahari Experimental Farm during performance test periods of approximately 180 days, were subjected to evaluation according to specific body measurements and breed standards. The respective body measurements included live weight, shoulder height, body length, body width, scrotal circumference and average daily weight gain (ADG). The measurements were recorded at the beginning and end of each evaluation period. The rams were evaluated visually by breed inspectors and classified according to breed standards. Live weight recorded at the beginning of evaluation periods, was moderate to high, and positively correlated with most of the body measurements recorded in the study. The exception was ADG, which was negatively correlated (-0.25) with live weight. Live weight recorded at the end of evaluation periods was moderately and positively correlated with scrotal circumference (0.57), and highly correlated with body width (0.76). Average daily weight gain was high and positively correlated with live weight (0.63) recorded at the end of the evaluation periods. Large variations within test periods, that could be attributed to age differences and variation between the respective groups of rams, necessitated correction of the data for the effect of year/season. The rams were divided into above- and below average groups according to their weight at the beginning of the test period. Analysis of the growth patterns of rams during the study period, using the adjusted end data, indicated that 75% of the rams remained in their respective groups. This finding supports the above-mentioned positive correlation of 0.58 that was reported for live weight at the beginning and end of evaluation periods. Body measurements recorded at the beginning of the evaluation periods were in most cases moderately to high and positively correlated (P<0.0001) with measurements recorded at the end of the evaluation periods. The exception was ADG, which was low and negatively correlated with the respective body measurements that were recorded at the beginning of evaluation periods. After each body measurement was quantified by the sum of squares, it became evident that year/season had the greatest influence on the body measurements recorded at the end of an evaluation period. Year/season influenced live weight, body width, shoulder height, scrotal circumference and ADG, with the largest effect on live weight and the least influence on ADG (R2 = 31.62). After correction for year/season, the contribution of the respective measurements to live weight recorded at the end of evaluation periods were still in the same order. After a step-wise procedure for adjusted live weight at the end of evaluation periods was performed, it was found that body width contributed the most to live weight. Average daily gain made the third largest contribution, i.e. with respect to body width (largest) and body length (second largest), to corrected live weight recorded at the end of evaluation periods. The high correlations reported between the respective body measurements necessitated the analysis of the data for multi-colinearity to determine whether the partial contribution of the respective body measurements would differ from the initial values. The partial contribution of the respective body measurements, however, was not affected by the analysis. Regression analysis indicated that the respective body measurements and weights recorded at the beginning of evaluation periods decreased significantly (P<0.0001) over the entire period (1988-2000). The largest annual decreases were reported for ADG (- 2.5%) and body width (-1.1%), respectively. The decreases in ADG and body width are indications that Dorper sheep decreased in size during the period of this study. The value of visual appraisal methods therefore need to be seriously considered to determine whether this is a valuable management tool to assess the production performance of Dorper rams under extensive conditions. The influence of corrected body measurements and ADG, i.e. after selection by breed inspectors and according to breed standards, was plotted on scatter plots. The plots indicated that the breed inspectors selected larger and heavier rams throughout. It is however, still the case in the Dorper industry that rams that perform below average are sometimes classified and used as stud breeding material. Body width and shoulder height were the body measurements that received the highest and lowest approval as visual selection criteria, respectively. A possible reason for this is that the tendency of a smaller Dorper sheep can be attributed to the decreasing shoulder height, as the latter is an indication of body frame size. Visual appraisal should therefore be used in conjunction with scientific methods. Optimum size for the Dorper should be established. / AFRIKAANSE OPSOMMING: ‘N BEOORDELING VAN PRESTASIE-EVALUERINGSPROSEDURES VAN DORPERRAMME ONDER EKSTENSIEWE BESTUURSTOESTANDE Dorperramme (n=2565) is vanaf 1988 tot 2000 te Kalahari Proefplaas in prestasietoetstydperke van ongeveer 180 dae onder ekstensiewe toestande volgens sekere liggaamsparameters en rasstandaarde geëvalueer. Die onderskeie liggaamsmates het lewende gewig, skouerhoogte, liggaamsbreedte, liggaamslengte, skrotumomtrek en gemiddelde daaglikse toename (GDT) ingesluit. Die parameters is aan die begin en einde van elke toetsperiode gemeet. Die ramme is visueel deur rasinspekteurs geëvalueer en volgens rasstandaarde geklassifiseer. Die lewende gewig van die ramme wat aan die begin van die toetsperiodes aangeteken is, was matig tot hoog en positief met meeste van die liggaamsparameters gekorreleerd. Die uitsondering was GDT, waar ʼn negatiewe korrelasie (-0.25) gevind is. Die lewende gewig van die ramme aan die einde van die toetsperiodes was matig en positief met beide skrotumomtrek (0.57) en die hoogste met liggaamsbreedte (0.76) gekorreleerd. Gemiddelde daaglikse toename was hoog en positief (0.63) met lewende gewig aan die einde van die toetsperiode gekorreleerd. Groot variasie binne toetsperiodes, wat aan ouderdomsverskille en variasie tussen toetsgroepe toegeskryf kan word, het genoodsaak dat die data vir die effek van jaar/seisoen gekorrigeer word. Ramme is volgens begingewig in bo- en ondergemiddelde groepe gerangskik. Groeipatrone van die ramme is met gekorrigeerde einddata ondersoek en gevind dat 75% van ramme gedurende die toetsperiode in hul onderskeie groepe gebly het. Hierdie bevinding verleen steun aan die positiewe korrelasie van 0.58 wat vir lewende gewig aan die begin en einde van toetsperiodes hierbo gevind is. Die liggaamsmates wat aan die begin en einde van die toetsperiodes gemeet is, was in die meeste gevalle matig tot hoog en positief (P < 0.0001) met mekaar gekorreleerd. Die uitsondering was GDT, wat laag en negatief met liggaamsmates, wat aan die begin van toetsperiodes aangeteken is, gekorreleerd was. Die seisoenseffek het die grootste bydrae tot lewende gewig aan die einde van die toetsperiodes gemaak, d.i. nadat elke meting deur die somme van kwadrate gekwantifiseer is. Dit is gevolg deur liggaamsbreedte, -lengte, skouerhoogte, skrotumomtrek en GDT (R² = 31.62%). Nadat daar vir jaarseisoen gekorrigeer is, was die bydrae van die onderskeie parameters tot lewende gewig aan die einde van toetsperiodes nog in dieselfde volgorde. Nadat ʼn stapsgewyse prosedure vir gekorrigeerde lewende gewig aan die einde van toetsperiodes gedoen is, is gevind dat liggaamsbreedte die grootste bydrae tot lewende gewig aan die einde van toetsperiodes gemaak het. Gemiddelde daaglikse toename het die derde grootste bydrae, d.i. na liggaamsbreedte en –lengte tot gekorrigeerde lewende gewig aan die einde van toetsperiodes gemaak. Vanweë die hoë korrelasies wat tussen die onderskeie liggaamsmates gevind was, is daar ook vir multi-kollineariteit getoets om vas te stel of die parsiële bydraes van die onderskeie liggaamsmetings verander. Dit het egter onveranderd gebly. Regressie-analises het getoon dat liggaamsmates geneem aan die begin en einde van die toetsperiodes betekenisvol (P<0.0001) oor die hele tydperk (1988 – 2000) afgeneem het. Die grootste jaarlikse afnames was vir GDT (-2,5%) en liggaamsbreedte (-1,1%) bereken. Die negatiewe waardes wat verkry is, toon dat die Dorper oor tyd kleiner word. Die toepaslikheid van die visuele seleksiemetodes moet dus ernstig bevraagteken word. Die invloed van gekorrigeerde liggaamsmates en GDT op seleksie volgens rasstandaarde wat deur die rasinspekteurs gedoen is, is toe op puntediagramme aangetoon. Dit het getoon dat die rasinspekteurs deurentyd groter en swaarder ramme vir stoetseleksie selekteer. Daar is egter steeds ramme wat ondergemiddeld presteer en wat aan die Dorperbedryf as stoetramme beskikbaar gestel word. Liggaamsbreedte was die liggaamsmate wat die hoogste voorkeur van rasinspekteurs gekry het. Die liggaamsmate wat die minste deur die rasinspekteurs in ag geneem is, was dié van skouerhoogte. ʼn Moontlike verklaring vir laasgenoemde is dat die tendens van die kleinerwordende Dorper hieraan gekoppel kan word, omdat skouerhoogte 'n aanduiding van raamgrootte is. Visuele beoordeling behoort in samewerking met wetenskaplike metodes gebruik te word om sodoende ʼn optimum grootte vir die Dorper daar te stel.

Thesis (MScAgric (Animal Sciences))--University of Stellenbosch, 1985. / The domestication of ostriches and commercial ostrich breeding has a long and interesting history. Orstriches were successfully domesticated in Algeria during 1857 and also successfully bred in Italy during 1859. In South Africa the domestication of wild ostriches started in the Cape Colony with 80 tame birds during 1865. This number increased to 32 247 birds within ten years (De Mosenthal, 1877). Ostrich farming reached a peak during 1913 with 757 000 birds (De Jager, 1985, personal communication). Currently (1985) there are approximately 120 000 domesticated ostriches in South Africa. The commercial production of ostrich meat started in 1958 and the first abattoir was built in 1964. At the present moment 85 000 birds are slaughtered anually. Although the income from the carcass of a 14 month old ostrich comprise only 15% of the total income of a 14 month old bird (With the hide comprising 65% and, the first harvest feathers 20%), the subjects of carcass characteristics and meat quality has long been neglected. Very little is known about the anatomy of the skeleton and muscles of the ostrich, the post mortem reactions of these muscles and the effects of different slaughtering, chilling and deboning practices. The necessity of the above mentioned have proved very usefull in the beef, sheep, pork and poultry industries. With this present study we described the essential skeletal and muscular anatomy and included well known practices to the meat industry, such as electrical stimulation of the carcasses, post mortem pH monotoring of certain muscles, different deboning practices and vacuum packed ageing of selected cuts of ostrich meat. The obtained results are in agreement with general meat science, with expected inter-species variation, e.g. where the final pH-values of pork is relatively low at approximately 5,5; that of beef is higher at 5,7 and ostrich meat even higher at 6,0. Certain other fields of essential research are pointed out in this study, such as the growth and development of the ostrich, as well as certain basic biochemical research on the meat of the ostrich.

Thesis (MScAgric) -- Stellenbosch University, 2009. / ENGLISH ABSTRACT: Ruminants have a compound stomach system that enables them to utilize forages more efficiently than monogastric animals. However, forages alone do not contain sufficient nutrients to meet the requirements of high producing dairy cows. Forages are high in fibre and their nutrient availability depends on the degree of cell wall degradability. Improvements in forage fermentation would increase energy intake and subsequently milk production and performance by dairy cows. It is therefore important to find ways to improve forage degradation and utilization in the rumen. The use of different non-fibre carbohydrate (NFC) sources has different effects on animal performance. Supplementing forage based diets with energy sources containing sugar, starch or pectin results in variation in performance measurements such as milk yield, milk composition and dry matter intake (DMI). This thesis reports on two studies in which the effect of energy supplementation on forage fermentation and digestion parameters was investigated. In the first study an in vitro gas production protocol was used to determine the effect of sugar (molasses), starch (maize meal) and pectin (citrus pulp) on total gas production and rate of gas production of different forages. The forage substrates included wheat straw (WS), oat hay, (OH) lucerne hay (LUC), ryegrass (RYE) and kikuyu grass (KIK). The three energy sources, as well as a control (no energy source) were incubated in vitro with each of the above mentioned forages. Rumen fluid was collected from two lactating Holstein cows receiving a diet consisting of oat hay, lucerne, wheat straw and a concentrate mix. Forages alone (0.25 g DM) and/or together (0.125 g DM) with either molasses (0.1412 g DM), citrus pulp (0.1425 g DM) or maize meal (0.125 g DM) were weighed into glass vials and incubated for 72 hours. The weights of the energy sources were calculated on an energy equivalent basis. Blank vials, that contained no substrates, were included to correct for gas production from rumen fluid alone. The substrates were incubated in 40 ml buffered medium, 2 ml of reducing solution and 10 ml rumen fluid. Gas pressure was recorded automatically every five minutes using a pressure transducer system and the method based on the Reading Pressure Technique (Mauricio et al., 1999). Gas pressure was converted to gas volume using a predetermined regression equation. In the first gas production trial, the gas production included gas produced by the energy sources, while in the second gas production trial, the energy source gas production was deducted from the total gas production to determine the effect of energy source on gas production of respective forage substrates per se. Data were fitted to two non-linear models adapted from Ørskov and McDonald (1979). Significant forage x energy interactions were observed for the non-linear parameter gas production (b) in Model 1 and for b and lag phase (L) in Model 2 in both trials. In the first gas production trial, the higher fermentability of the energy sources supplemented to forage substrates, increased b (Model 1 & 2) of the LUC and WS. The gas production rate was affected in different ways for different forages, with the most noticeable effect on WS when it was supplemented with energy sources. All the energy sources increased c of WS irrespective of the model used. Energy sources had no effect on the L of LUC, OH or RYE, but decreased the L of WS and KIK. In the second trial, maize meal had no effect on b for any of the forages (Model 1 & 2), while molasses (Model 1 & 2) decreased b for all forage substrates, and citrus pulp (Model 1 & 2) decreased b of OH and RYE, to lower values than those of the control treatments. Gas production rate was not affected by molasses for any of the forage substrates, while citrus pulp (Model 1 & 2) increased c of OH and maize meal increased c of OH and KIK. Lag phase was only affected by energy sources in WS and KIK, where all the energy sources had lower L values than the control treatment. It was concluded that forage fermentability is affected differently by different energy sources. These observations may have important implications, in practice, on rumen health and milk production, and the data obtained can potentially be used as guidelines in feed formulations. In the second study, in vitro digestibility trials were undertaken to determine the effect of sugar (molasses and sucrose), starch (maize meal and maize starch) and pectin (citrus pulp and citrus pectin) on neutral detergent fibre (NDF) and dry matter (DM) degradability of forages. Forage substrates used included wheat straw, oat hay, lucerne hay, ryegrass and kikuyu grass. Rumen fluid was collected from two lactating Holstein cows receiving a diet consisting of oat hay, wheat straw and a concentrate mix. In vitro degradability was done with an ANKOM Daisy II incubator and forage substrates were incubated with or without the respective energy sources for 24, 48 and 72 hours. The substrates were incubated in 1076 ml buffered medium, 54 ml of reducing solution and 270 ml rumen fluid. The residues were washed, dried and analyzed for NDF. In the study with the applied energy sources (molasses, maize meal and citrus pulp) there were a forage x energy source interactions. Supplementation with the applied energy sources all improved dry matter degradability (DMD) of forages (24 and 72 hours), when compared to the control treatment, except for RYE supplemented with maize meal and citrus pulp at 24 hours. Molasses seemed to have had the biggest effect on DMD in all forage substrates. Supplementation with maize meal had no effect on neutral detergent fibre degradability (NDFD) of any forage substrate, except for an improvement in NDFD of LUC at 72 hours. Molasses improved NDFD of LUC at 24h, but had no effect on the other forage substrates. Citrus pulp improved NDFD of OH (72 hours), as well as LUC and WS (24 and 72 hours). It is postulated that the NDF of the energy sources was more digestible than that of the respective forages, and that the improved NDFD values could be ascribed to the contribution of the energy source NDFD. Overall, pasture grasses had a higher NDFD than the hays and straw, and appear to be more readily fermentable by rumen microbes than the low quality hays and straw explaining the higher NDFD. In the study involving the purified energy sources (sucrose, maize starch and citrus pectin), forage x energy source interactions were observed. In general, supplementation with these energy sources improved DMD at 24 and 72 hours except for RYE and KIK (72 hours). Pasture grasses (RYE and KIK) had a higher NDFD than LUC, OH and WS. At 72 hours, NDFD was 37.1% for LUC, 42.5% for OH and 40.3% for WS, compared to 70.5% for KIK and 64.9% for RYE. A possible explanation is that KIK and RYE samples came from freshly cut material, harvested after a 28d re-growth period. In general, sucrose (24 and 72 hours) and citrus pectin (72 hours) had no effect on NDFD of forage substrates. However, supplementing oat hay (24 hours) with starch and citrus pectin, and wheat straw (24 and 72 hours) with starch lowered NDFD, when compared to the control treatment. It is hypothesized that microbes fermented the easily fermentable energy sources first, before attacking forage NDF. The study suggested that forage NDFD values are not fixed, and may be altered by type of energy supplementation. / AFRIKAANSE OPSOMMING: Die meervoudige maagsisteem van herkouers stel hulle in staat om ruvoer meer effektief te benut as enkelmaagdiere. Ruvoere alleen bevat egter nie genoeg voedingstowwe om die behoeftes van hoogproduserende melkbeeste te bevredig nie. Ruvoere is ryk aan vesel en hul voedingstofbeskikbaarheid word bepaal deur die graad van selwand degradeerbaarheid. ‘n Verhoging in ruvoerfermentasie sal energieinname verhoog en gevolglik ook melkproduksie en prestasie. Dit is dus belangrik om maniere te vind om ruvoerdegradeerbaarheid en -verbruik in die rumen te verbeter. Die gebruik van verskillende nie-vesel koolhidraat (NFC) bronne het verskillende uitwerkings op die prestasie van diere. Energie-aanvullings soos suiker, stysel en pektien tot ruvoer-gebasseerde diëte, beïnvloed prestasiemaatstawwe soos melkproduksie, melksamestelling en droëmateriaalinname (DMI) op verskillende maniere. Hierdie tesis lewer verslag oor twee studies waar die invloed van energie-aanvullings op ruvoerfermentasie en verteringsmaatstawwe ondersoek is. In die eerste studie is ‘n in vitro gasproduksieprotokol gebruik om die invloed van suiker (melasse), stysel (mieliemeel) en pektien (sitruspulp) op totale gasproduksie (b) en tempo van gasproduksie (c) van verskillende ruvoersubstrate te bepaal. Ruvoersubstrate wat gebruik is, was koringstrooi (WS), hawerhooi (OH), lusernhooi (LUC), raaigras (RYE) en kikuyugras (KIK). Die drie energiebronne, sowel as ‘n kontrole (geen energiebron), is in vitro geïnkubeer saam met elk van die genoemde ruvoere. Rumenvloeistof is verkry van twee lakterende Holsteinkoeie, wat ‘n dieet ontvang het bestaande uit hawerhooi, koringstrooi en ‘n kragvoermengsel. Ruvoere is alleen en/of in kombinasie met melasse (0.1412 g DM), sitruspulp (0.1425 g DM) of mieliemeel (0.125 g DM) in glasbottels afgeweeg en vir 72 uur geïnkubeer. Die massas van die energiebronne is op ‘n energie-ekwivalente basis bereken. Leë bottels wat geen substraat bevat het nie, is ingesluit om te korrigeer vir gasproduksie afkomstig vanaf rumenvloeistof alleen. Substrate is in 40 ml van ‘n buffermedium, 2 ml reduserende oplossing en 10ml rumenvloeistof geïnkubeer. Gasdruk is elke vyf minute outomaties aangeteken deur gebruik te maak van ‘n drukmetersisteem en die metode is gebasseer op die Reading gasdruktegniek. Gasdruk is omgeskakel na gasvolume deur gebruik te maak van ‘n voorafbepaalde regressievergelyking. In die eerste proef het totale gasproduksie die gas wat deur die onderskeie energiebronne geproduseer is, ingesluit. In die tweede proef is gasproduksie afkomstig van die energiebronne afgetrek van totale gasproduksie, om sodoende die invloed van die energiebronne per se op die gasproduksie van die onderskeie ruvoersubstrate, te bepaal. Data is met behulp van twee nie-liniëre modelle gepas. Betekenisvolle ruvoer x energie-interaksies is in albei proewe waargeneem vir die nie-liniëre parameter b (gasproduksie) in Model 1, en vir b en L (sloerfase) in Model 2. In die eerste proef het die energiebronne se hoë fermentasie gelei to ‘n verhoging in b (Model 1 & 2) van LUC en WS. Energie-aanvullings het die c-waarde van die onderskeie ruvoere verskillend beïnvloed, met WS wat die mees opvallende effek gehad het. Al die energiebronne het die c-waarde van WS verhoog, ongeag watter model gebruik is. Energiebronne het geen invloed op die L-waarde van LUC, OH of RYE gehad nie, maar het wel die L-waarde van WS en KIK verlaag. In die tweede proef het mieliemeel geen invloed op die b-waarde van enige van die ruvoere gehad nie (Model 1 & 2), terwyl melasse (Model 1 & 2) die b-waarde van alle ruvoere verlaag het, en sitruspulp (Model 1 & 2) OH en RYE se b waardes verlaag het tot laer as die kontroles. Melasse het geen invloed op die c-waarde van die onderskeie ruvoersubstrate gehad nie, terwyl sitruspulp (Model 1 & 2) die c-waarde van OH, en mieliemeel die c-waarde van OH en KIK, verhoog het. Energiebronne het slegs ‘n invloed op die sloerfase in WS en KIK gehad, waar dit L verlaag het tot laer waardes as dié van die kontroles. Daar is gevind dat ruvoer-fermenteerbaarheid verskillend beïnvloed word deur verskillende energiebronne. Bogenoemde resultate kan in die praktyk betekenisvolle invloede hê op rumengesondheid en melkproduksie en die data wat verkry is, kan potensieël gebruik word as riglyne in voerformulerings. In die tweede studie is in vitro verteerbaarheidsproewe gedoen om die effek van suiker (molasse en sukrose), stysel (mieliemeel en mieliestysel) en pektien (sitruspulp en sitrus-pektien) op neutraalonoplosbare vesel (NDF) en droë materiaal (DM) degradeerbaarheid van ruvoere, te bepaal. Ruvoersubstrate wat gebruik is, was WS, OH, LUC, RYE en KIK. Rumen vloeistof is verkry van twee lakterende Holstein koeie, wat ‘n dieet ontvang het bestaande uit hawerhooi, koringstrooi en ‘n konsentraat mengsel. Die in vitro degradeerbaarheidsproef is gedoen met ‘n ANKOM Daisy II inkubator. Ruvoersubstrate is geïnkubeer met of sonder die onderskeie energiebronne vir 24, 48 en 72 uur. Die substrate is geïnkubeer in 1076 ml buffer medium, 54 ml reduserende oplossing en 270 ml rumen vloeistof. Residue is gewas, gedroog en geanaliseer vir NDF. In die proef met toegepaste energiebronne (molasse, mieliemeel en sitruspulp), was daar ruvoer x energiebron interaksies. Toegepaste energiebron aanvullings het almal DMD van ruvoersubstrate (24 en 72 uur) verbeter, uitsluitend vir RYE wat aangevul is met mieliemeel (24 uur) en sitruspulp (24 uur). Van al die ruvoersubstrate het molasse die grootste effek gehad op DMD. Mieliemeel aanvullings het geen effek gehad op neutraal-onoplosbare vesel degradeerbaarheid (NDFD) van ruvoersubstrate nie, behalwe vir ‘n verbetering in NDFD van LUC by 72 uur. Molasse het NDFD van lucern by 24 uur verbeter, maar geen effek gehad op ander ruvoersubstrate nie. Sitruspulp het NDFD van OH (72 uur), asook LUC en WS (24 & 72 uur) verbeter. Daar word beweer dat die NDF van energiebronne meer verteerbaar is as die van ruvoersubstrate, en dat die verbetering in NDFD waardes toegeskryf kan word aan die bydraes van energiebronne se NDFD. Weidingsgrasse (RYE & KIK) het oor die algemeen ‘n hoër NDFD as hooie en strooi gehad. Rumen mikrobes blyk ook om dié grasse vinniger te verteer as lae kwaliteit hooie en strooi, wat gevolglik die hoër NDFD verduidelik. In die proef met suiwer energiebronne (sukrose, mieliestysel en sitrus-pektien) is ruvoer x energiebron interaksies waargeneem. Energiebronaanvullings het DMD by 24 en 72 uur verbeter, buiten vir RYE en KIK (72 uur). Weidingsgrasse het hoër NDFD as LUC, OH en WS. By 72 uur was die NDFD van LUC 37.1%, OH 42.5%, WS 40.3%, in vergelyking met 70.5% vir KIK en 64.9% vir RYE. ‘n Moontlike verklaring vir die hoër NDFD van KIK en RYE, is omdat dit vars gesnyde material is, geoes na slegs 28 dae hergroei. Oor die algemeen het sukrose (24 & 72 uur) en sitrus-pektien (72 uur) geen effek gehad op NDFD van ruvoersubstrate nie, terwyl stysel en pektien aanvullings tot OH (24 uur), en stysel aanvullings tot WS (24 & 72 uur) NDFD verlaag het. Daar word hipotetieseer dat mikrobes eers die vinnig fermenteerbare energiebronne fermenteer, voordat hulle ruvoer NDF aanval. Hierdie studie beweer dat ruvoer NDFD waardes nie vas is nie, en dat dié waardes beïnvloed mag word deur energiebron aanvullings.

Thesis (MScAgric)--Stellenbosch University, 1999. / ENGLISH ABSTRACT: The rate of hydrolysis of urea in the rumen of animals is a major limitation when considering the substitution of natural protein with urea in the formulation of rations. The aim of this study was to evaluate polymer coated urea prills with variable coating thickness and evaluate its potential as a slow-release NPN compound. A new slowrelease urea compound, made by coating prilled feedgrade urea with a co-polymer of urea-formaldehyde resin and a castor-coconut alkyd was initially evaluated for urea-nitrogen concentration in distilled water in order to evaluate its potential as a slow-release urea product for ruminants. Amino/alkyd or polyester blends are among the cheapest of the modern synthetic systems and are considered because it is non-toxic, low-cost, biodegradable and easy to manufacture. A 2 x 2 x 2 x 2 factorial design was used and 16 individual products were made and evaluated. The Wurster method was used to encapsulate urea prills. The slopes of the urea release curves represented the release rate of the encapsulated products and were compared to identify the process variables, which had an effect on release rate. Two of the coating variables, coating weight and alkyd: resin ratio, had a major effect (P = 0.0001) on the release rate of urea. The crushing strength of encapsulated products was significantly (P = 0.0001) higher than that of untreated urea. Results motivated the evaluation of the products in the rumen of sheep in terms of rumen ammonia and blood urea N concentrations. Four slow-release products were made after interpreting results from the first study, and differed on account of the coating weight and the composition of the co-polymer. Fifteen fistulated wethers were randomly allotted into 5 groups and intraruminally received an equivalent of 15g urea. Rumen ammonia and blood ammonia were taken at 0, 1, 2, 4, 6, 8, 12, 16, 20, 24, 36 and 48 h after administration of the various treatments. Slow release urea (SRU) resulted in significantly lower rumen ammonia peaks (P = 0.0001) than untreated urea, while the peaks were also significantly delayed. Untreated urea resulted in the maximum concentration at two hours after administration of the urea (P = 0.0685) while the SRU's reached a maximum at six hours after administration in the rumen. No significant differences between the four different SRU types were found. Responses in blood urea-N was similar to that observed for rumen ammonia nitrogen. The encapsulation was effective in decreasing the rate of ammonia release from the urea for up to six hours after administration. In a third trial four Dehne Merino wethers were used in a 2 x 2 Latin square design. They received a SRU product equivalent to 0.4 g urea per kg body weight orally. Rumen liquor and blood samples were taken at 0, 1, 2, 4, 6, 8, 12, 16, 20, 24, 36 and 48 h after intake. Difficulty was initially experienced with ingestion and palatability of the SRU products due to the strong formaldehyde and butanone odour present in the coating. The maximum rumen ammonia (NH3) concentration for the SRU were lower than that of untreated urea (17.5 mg N/dl vs. 66.9 mg N/dl). The time to reach blood urea levels also differed considerably (6 h vs. 24 h for blood urea nitrogen) between treatments. The encapsulation of urea prills shows potential solutions to reduce the solubility of urea and also reduce the hygroscopic nature of urea and improve the palatability and storage characteristics thereof. Keywords: Slow-release urea, encapsulate, copolymer, urea formaldehyde rumen ammonia, blood urea nitrogen, solubility, palatability, storage characteristics. / AFRIKAANSE OPSOMMING: Die vinnige tempo waarteen ureum in die rumen na ammoniak omgesit word, is die grootste beperking in die optimale benutting van ureum, as vervanging van natuurlike protein in herkouerrantsoene. Die doel van hierdie studie was om 'n stadig vrystellende ureumproduk te ontwikkel wat die rumenammoniakvlak gedurende 'n aansienlike periode van die dag bokant 'n sekere vlak kan hou. 'n Nuwe stadig vrystellende ureumproduk, vervaardig deur ureumkorrels met 'n kopolimeer van ureum-formaldehiedhars en 'n kastor-en klapperalkied te bedek, is geevalueer om die potentiaal as stadig vrystellende nie-protein stikstof (NPN)-produk vir herkouers te ondersoek. Die veiligheid, biodegradeerbaarheid, lae koste en maklike vervaardiging van amino/alkied-kopolimere maak dit een van die goedkoopste sintetiese sisteme om vir stadig vrystellende sisteme te oorweeg. 'n 2 x 2 x 2 x 2 Faktoriale antwerp is gebruik om 16 individuele produkte te vervaardig. Die Wurster-metode is gebruik om individuele korrels te enkapsuleer met die polimeer en die potentiaal van die produkte is aanvanklik geevalueer deur die ureumstikstofvrystelling in gedistilleerde water te meet. Die hellings van die vrystellingsgrafieke is vergelyk om die veranderlikes te bepaal wat die grootste invloed op die vrystellingstempo van ureum uit die ge·inkapsuleerde produkte het. Resultate dui dat twee verandelikes 'n betekenisvolle effek het op die vrystellingstempo, nl. dikte van die omhulsel, en die samestelling van die kopolimeer (P = 0.0001 en P = 0.0135, onderskeidelik) het. Die samedrukbaarheid van die ge ·lnkapsuleerde produkte was ook betekenisvol hoer (P = 0.0001) as die van onbehandelde ureumkorrels, wat lei tot verbeterde bergings- en hanteringseienskappe. lnterpetering van resultate lei tot die vorming van vier stadig vrystellende produkte. Vyf groepe van 3 volwasse rumengefistuleerde Dohnemerinohamels is in 'n proef gebruik om die potentiaal van die produkte verder te ondersoek. 'n Ekwivalent van 15 g ureum is direk in die rumen van elke dier geplaas en ammoniak-en bloed monsters is 0, 1, 2, 4, 6, 8, 12, 16, 20, 24, 36 & 48 ure na dosering geneem. Die stadig vrystellende ureumprodukte het 'n betekenisvol laer maksimum waardes vir beide ammoniak-en bloedstikstofureum getoon (P = 0.0001 ). Onbehandelde ureum het 'n maksimum rumenammoniakstikstof konsentrasie reeds twee ure na toediening bereik in vergelyking met ses ure vir die stadig vrysellende produkte. Geen betekenisvolle verskille in hierdie parameters is tussen die ge·lnkapsuleerde produkte gevind nie, terwyl geen interaksie is tussen hoofeffekte voorgekom het nie. In 'n derde proef is vier Dohnemerinohamels gebruik om die vrystellingstempos, in terme van rumenammoniak-en bloedureumstikstof te bepaal waar die stadig vrystellende produk en onbehandelde ureum direk aan die diere gevoer is . 'n Ekwivalent van 0.4 g ureum/kg liggaamsmassa is gevoer. Aanvanklik is inname- en smaakliksheidprobleme ondervind, moontlik as gevolg van die sterk butanoon-en formaldehiedreuk van die omhulsel. Die rumenammoniakstikstof het 'n laer maksimum (17.5 vs. 66.9 mg N/1 00 ml) as die van onbehandelde ureum gehad terwyl die tyd wanneer maksimum konsentrasie bereik word ook aansienlik later was. Die polimeer inkapsulering van ureumkorrels toon potensiaal as 'n stadig vrystellende ureumproduk deurdat dit die oplosbaarheid van ureum in die rumen verlaag. Bykomende voordele is dat die omhulsel die higroskopisiteit verlaag en die samedrukbaarheid verhoog, beide eienskappe wat die hantering-en bergingseienskappe bevorder. Sleutelwoorde: Stadig vrystellende ureum, enkapsulering, kopolimeer, ureumformaldehied, rumenammoniak, bloed ureum stikstof, oplosbaarheid, smaaklikheid, bergingseienskappe.