No / The study investigated the role of endothelin (ET) and the ET receptor subtypes ETA and ETB in mediating longitudinal contraction in the mouse proximal and distal colon. Cumulative concentration¿response curves to a range of ET agonists (ET-1, ET-2, ET-3, (Ala1,3,11,13) ET and IRL 1620) were established by administering concentrations ranging from 0.01nM to 0.3¿M. Concentration¿response curves to ET-1, which exhibits a high affinity for both ETA and ETB receptor subtypes, were also established in the presence of the ETA antagonist BMS 182874 and the ETB antagonist IRL1038. The addition of the selective ETA receptor antagonist BMS 182874 caused a rightward shift of the concentration¿response curve to ET-1 in both sections of the colon. The ETB receptor antagonist IRL1038 (0.3¿1¿M) did not significantly effect the response to ET-1 in the proximal colon but caused a significant decrease in response towards higher concentrations ranges (3nM) in the distal colon. A comparison of the concentration¿response curves to ET-1, ET-2 and ET-3 showed a rank order of potency ET-1ET-2ET-3 in the proximal colon and ET-1ET-2ET-3 in the distal colon. The selective ETB receptor agonists, (Ala1,3,11,13) ET and IRL 1620 did not produce any response in the proximal sections of the colon but produced a smaller contraction in the distal segments. The data indicate that ET can contract the proximal tissues of the mouse colon predominantly via ETA receptors and in the distal tissues via ETA and ETB receptors.

Made available in DSpace on 2014-06-11T19:23:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2001Bitstream added on 2014-06-13T18:51:02Z : No. of bitstreams: 1 silva_dr_me_botfm.pdf: 2448429 bytes, checksum: 2109583be6db8fca687c468e5def8fe6 (MD5) / Este trabalho investigou o processo de reparação de uma ferida cirúrgica do cólon distal de ratos tratados com etanol. Foi utilizado o estudo da força de ruptura, avaliação histológica, quantificação de fibroblastos e análise morfométrica da área da cicatriz. Foram utilizados 126 ratos distribuídos por sorteio em 02 grupos: Grupo A1-controle e Grupo A2- etanol. Os animais do grupo A1 receberam ração e água “ad libitum” por 60 dias, após este período 12 animais foram sacrificados e submetidos ao estudo da força de ruptura. Os demais foram operados e submetidos a uma anastomose do cólon distal e estudados no 7o, 14o e 21o dias pósoperatório. Os animais do grupo A2 receberam ração e solução hidroalcoólica a 30% por 60 dias. Após este período foram submetidos ao mesmo procedimento do grupo controle; receberam ração e água no período pósoperatório, até o final do experimento. Verificou-se que a força de ruptura do segmento intestinal estudado foi menor em animais não operados tratados com etanol (A2M0) e, também foi menor nos animais tratados com etanol, operados e estudados no 14o dia pós-operatório (A2M2) quando comparados ao controle. A análise morfométrica da área da cicatriz demonstrou valores significantemente maiores nos animais tratados com etanol e estudados no 7o dia pós-operatório (A2M1) quando comparados ao controle... / This study investigated the healing process of a distal colon surgical wound in rats treated with ethanol. The rupture strength, histological evaluation, fibroblast quantification and morphometric analysis of the healing tissue were performed. One hundred and twenty-six rats were randomized into 2 groups: Group A1 – control group and Group A2 – ethanol group. Group A1 animals received food and water ad libidum for 60 days, after this period, 12 animals were sacrificed and underwent an evaluation of rupture strength. The remaining rats were operated and underwent distal colon anastomosis and were evaluated on the 7th /14th and 21st postoperative days. Group A2 animals received food and hydroalcoholic solution at 30% for 60 days. After this period they underwent the same procedure used for the control group. In the postoperative period they received food and water until the end of the investigation. It was observed that the rupture strength of the studied intestinal segment was lower in the animals which were not operated and treated with ethanol (A2T0). This strength was also lower in the animals which were operated and treated with ethanol evaluated on the 14th postoperative day (A2T2), when compared to the control group... (Complete abstract, click electronic access below)

Orientador: Shoiti Kobayasi / Resumo: Este trabalho investigou o processo de reparação de uma ferida cirúrgica do cólon distal de ratos tratados com etanol. Foi utilizado o estudo da força de ruptura, avaliação histológica, quantificação de fibroblastos e análise morfométrica da área da cicatriz. Foram utilizados 126 ratos distribuídos por sorteio em 02 grupos: Grupo A1-controle e Grupo A2- etanol. Os animais do grupo A1 receberam ração e água "ad libitum" por 60 dias, após este período 12 animais foram sacrificados e submetidos ao estudo da força de ruptura. Os demais foram operados e submetidos a uma anastomose do cólon distal e estudados no 7o, 14o e 21o dias pósoperatório. Os animais do grupo A2 receberam ração e solução hidroalcoólica a 30% por 60 dias. Após este período foram submetidos ao mesmo procedimento do grupo controle; receberam ração e água no período pósoperatório, até o final do experimento. Verificou-se que a força de ruptura do segmento intestinal estudado foi menor em animais não operados tratados com etanol (A2M0) e, também foi menor nos animais tratados com etanol, operados e estudados no 14o dia pós-operatório (A2M2) quando comparados ao controle. A análise morfométrica da área da cicatriz demonstrou valores significantemente maiores nos animais tratados com etanol e estudados no 7o dia pós-operatório (A2M1) quando comparados ao controle... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: This study investigated the healing process of a distal colon surgical wound in rats treated with ethanol. The rupture strength, histological evaluation, fibroblast quantification and morphometric analysis of the healing tissue were performed. One hundred and twenty-six rats were randomized into 2 groups: Group A1 - control group and Group A2 - ethanol group. Group A1 animals received food and water ad libidum for 60 days, after this period, 12 animals were sacrificed and underwent an evaluation of rupture strength. The remaining rats were operated and underwent distal colon anastomosis and were evaluated on the 7th /14th and 21st postoperative days. Group A2 animals received food and hydroalcoholic solution at 30% for 60 days. After this period they underwent the same procedure used for the control group. In the postoperative period they received food and water until the end of the investigation. It was observed that the rupture strength of the studied intestinal segment was lower in the animals which were not operated and treated with ethanol (A2T0). This strength was also lower in the animals which were operated and treated with ethanol evaluated on the 14th postoperative day (A2T2), when compared to the control group... (Complete abstract, click electronic access below) / Mestre

No description available.

Este projeto, analisou a distribuição dos neurônios nos plexos mioentérico (PM) e submucoso (PS) imunorreativos aos receptores P2X2 (ir) e P2X7 (ir), calbindina (Calb-ir), calretinina (Calr-ir), colina acetil transferase (ChAT-ir) e ao óxido nítrico sintase (NOS-ir) do colo distal de ratos submetidos à desnutrição a renutrição protéica. Utilizaram-se colos distais de ratos nutridos (N42), desnutridos (D42) e renutridos (RN42). Os resultados do plexo PM, demonstraram que 100% dos neurônios Calb-ir, Calr-ir, ChAT-ir e NOS-ir, expressavam os receptores P2X2-ir e P2X7-ir nos três grupos. A densidade neuronal no PM, demonstrou um aumento de 20% a 97% dos neurônios receptores P2X2-7-ir, Calr-ir, ChAT-ir e NOS-ir e no PS foi de 29% a 75%, ambos D42 e recuperação no RN42. O perfil neuronal P2X7-ir, Calb-ir, Calr-ir e ChAT-ir do PM demonstrou diminuição de 28% a 40% e no PS os neurônios P2X2-7-ir, Calb-ir e ChAT-ir de 19% a 47% no D42. Concluí-se que, a desnutrição afeta os neurônios entéricos havendo recuperação na renutrição, podendo influenciar nas funções gastrintestinais. / The aim of the work was to analyze the distal colon myenteric (MN) and submucous (SN) neurons immunoreactive for P2X2-7 receptors, calbindin (Calb-ir), calretinin (Calr-ir), choline acetyltransferase (ChAT) and nitric oxide synthase (NOS) of the animals submitted to undernutrition and refeeding proteic. Distal colon was used from nourished (N42), undernourished (D42) and refeeding (RN42) rats. The results have shown 100% coexpression of the myenteric and submucous Calb-ir, Calr-ir, ChAt-ir e NOS-ir neurons with P2X2-7-ir receptors. The MN density have shown increase of the 20% and 97% of the P2X2-7-ir, Calr-ir, ChAT-ir e NOS-ir neurons of the D42 group, and the SN have been increased 29% a 75% in the D42 group. In the MN neuronal profile have shown decrease P2X7-ir, Calb-ir, Calr-ir and ChAT-ir neurons of the 28% to 40% and in the PS P2X2-7-ir, Calb-ir and ChAT-ir of the 19% a 47% neurons in the D42 group. I concluded that, the undernutrition affects the enteric neurons and there was recuperation in the refeeding, this can influence the gastrintestinal functions.

Este projeto, analisou a distribuição dos neurônios nos plexos mioentérico (PM) e submucoso (PS) imunorreativos aos receptores P2X2 (ir) e P2X7 (ir), calbindina (Calb-ir), calretinina (Calr-ir), colina acetil transferase (ChAT-ir) e ao óxido nítrico sintase (NOS-ir) do colo distal de ratos submetidos à desnutrição a renutrição protéica. Utilizaram-se colos distais de ratos nutridos (N42), desnutridos (D42) e renutridos (RN42). Os resultados do plexo PM, demonstraram que 100% dos neurônios Calb-ir, Calr-ir, ChAT-ir e NOS-ir, expressavam os receptores P2X2-ir e P2X7-ir nos três grupos. A densidade neuronal no PM, demonstrou um aumento de 20% a 97% dos neurônios receptores P2X2-7-ir, Calr-ir, ChAT-ir e NOS-ir e no PS foi de 29% a 75%, ambos D42 e recuperação no RN42. O perfil neuronal P2X7-ir, Calb-ir, Calr-ir e ChAT-ir do PM demonstrou diminuição de 28% a 40% e no PS os neurônios P2X2-7-ir, Calb-ir e ChAT-ir de 19% a 47% no D42. Concluí-se que, a desnutrição afeta os neurônios entéricos havendo recuperação na renutrição, podendo influenciar nas funções gastrintestinais. / The aim of the work was to analyze the distal colon myenteric (MN) and submucous (SN) neurons immunoreactive for P2X2-7 receptors, calbindin (Calb-ir), calretinin (Calr-ir), choline acetyltransferase (ChAT) and nitric oxide synthase (NOS) of the animals submitted to undernutrition and refeeding proteic. Distal colon was used from nourished (N42), undernourished (D42) and refeeding (RN42) rats. The results have shown 100% coexpression of the myenteric and submucous Calb-ir, Calr-ir, ChAt-ir e NOS-ir neurons with P2X2-7-ir receptors. The MN density have shown increase of the 20% and 97% of the P2X2-7-ir, Calr-ir, ChAT-ir e NOS-ir neurons of the D42 group, and the SN have been increased 29% a 75% in the D42 group. In the MN neuronal profile have shown decrease P2X7-ir, Calb-ir, Calr-ir and ChAT-ir neurons of the 28% to 40% and in the PS P2X2-7-ir, Calb-ir and ChAT-ir of the 19% a 47% neurons in the D42 group. I concluded that, the undernutrition affects the enteric neurons and there was recuperation in the refeeding, this can influence the gastrintestinal functions.