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Cattle consuming low-quality forage may have decreased forage organic matter intake as a result of decreased nitrogen (N) intake. To date, a value has not been assigned to dried distillers' grains as a protein supplement to cattle consuming low-quality Bermudagrass forage. To address this shortcoming in the data, 13 duodenally and ruminally fistulated steers were arranged in an incomplete 13 x 4 Latin square with 13 treatments and 4 periods. Treatments were arranged as a 4 x 3 factorial plus a negative control (NC), which received no supplement. The first factor consisted of 4 levels of supplemental protein provided at 52, 104, 156, and 208 mg N/kg BW. The second factor consisted of one of three supplemental protein sources, cottonseed meal (CSM), dried distillers' grains (DDG), and dried distillers' grains plus urea (DDGU). Total digestible organic matter (TDOMI), and total organic matter intake (TOMI) increased in response to the increasing level of supplemental protein (P <0.01). Similarly, digestible neutral detergent fiber intake (DNDFI) increased as a result of supplementation (P = 0.06). Forage organic matter intake did not increase as a result of protein supplementation (P = 0.20). However, forage organic matter intake (FOMI) responded quadratically to provision of CSM (P = 0.02). In contrast, DDG and DDGU did not significantly increase FOMI. Organic matter digestibility (OMD) tended to increase (P= 0.09) as a result of protein supplementation. Ruminal ammonia concentrations increased linearly in response to increasing provision of supplemental protein and were greater than control steers (P less than 0.01). Supplementation with DDGU resulted in the greatest increase in ruminal ammonia concentrations. Plasma urea nitrogen (PUN) concentrations increased in a linear fashion in response to CSM and DDGU supplementation (P less than 0.01), while provision of DDG resulted in a quadratic response (P = 0.08). Based on these results, DDG can be utilized as a protein supplement to increase TDOMI, however, it accomplishes this without significantly impacting FOMI which is in contrast to CSM.

AN ABSTRACT OF THE THESIS OF David Williams for the Master of Ruminant Nutrition degree in Animal Science, presented on December 12, 2016 at Southern Illinois University Carbondale. TITLE: IN VITRO APPARENT RUMINAL DIGESTIBILITY OF DIETS CONTAINING CORN DISTILLERS GRAIN WITH VARYING LEVELS OF CRUDE FAT MAJOR PROFESSOR: Dr. Rebecca Atkinson
 Four dual-flow continuous fermenters were used in a Latin square design to determine the apparent ruminal digestibility and ruminal characteristics of diets containing dried distillers grains plus solubles (DDGS) at various levels of fat content. Fermenters were randomly assigned to one of the following treatments: 1) 40% DDGS containing 4.82% fat content (40 LOW); 2) 40% DDGS plus corn oil to obtain 7.5% fat (40 MED); 3) 40% DDGS plus corn oil to obtain 10.5% fat (40 HIGH); or 4) 70% DDGS plus corn oil to obtain 7.5% fat (70 MED). Rumen fluid was collected at the beginning of each period from two ruminally cannulated Angus cows previously adapted to the 40LOW treatment. Each period consisted of 10 days with a seven day adaptation period followed by three days of sample collection. Calories per gram of diet increased as percent fat increased and calories per gram was greater at the 70% inclusion of DDGS compared to 40% inclusion of DDGS at all levels of fat content. However, level of fat in the diet did not influence (P ≥ 0.35) apparent ruminal digestibility of DM, NDF, ADF, CP or total calories. Similarly, inclusion rate of DDGS had no influence (P ≥ 0.35) on nutrient digestibility. Ammonia concentrations were greatest (P = 0.0002) for 70 MED compared to the other treatments. However, treatment had no impact (P ≥ 0.16) on volatile fatty acid production with the exception of propionate which increased (P =0.05) as the level of DDGS increased from 40 to 70% inclusion rate. This data would suggest that level of fat content of DDGS has no negative influence on apparent ruminal digestibility and select ruminal characteristics. From an economic perspective, higher fat DDGS should have a higher price differential, but lower fat DDGS can still be an effective protein and energy substitute.

The objective of this study was to evaluate the lighter fraction of DDGS as a possible feedstuff for cattle. To accomplish this, a digestion trial was performed to determine nutrient digestibility and crude protein retention by steers consuming the lighter fraction of DDGS. Steers received L, a diet containing the lighter fraction of DDGS, D, a diet containing whole DDGS, or C, a control diet. Steers receiving L consumed less DM than steers receiving D and C. CP retention and digestibilities of DM, OM, ash, NDF, ADF, HC, and energy did not differ among steers in different treatment groups. Fat digestibility was greatest for steers consuming L and D. Steers consuming L digested more CP than steers receiving C, with steers consuming D being in between. These data suggest that the lighter fraction of DDGS can be effectively fed to cattle without adversely affecting digestibility.

The ethanol industry in Western Canada has seen significant growth in recent times spurred on mainly be environmental considerations. For a region with substantial grain production, increased prices from additional demand by the biofuel industry may inure to the benefit of grain farmers and land owners in the long term. The livestock industry however remains in a complex position facing the possibility of higher feed costs on the one hand and potential savings in feed cost on the other, with the availability of distillers grains- a by-product from ethanol production. The sectorial implications for the livestock sector could also be diverse and dependent upon the capacity to incorporate the distillers grains into the different feed rations. There is also the possibility of a spill-over effect from the US distillers grains market. This study therefore sought to complement current nutritional research by providing an economic perspective of the impact of distillers grains on the livestock industry in Western Canada. Focussing primarily on the beef cattle and hog industries, the study applied both linear programming and time-series techniques to assess potential benefits and costs. Potential positive economic benefits were observed for the inclusion of wheat and corn distillers grains with the former having a higher economic value in the high-protein feed segments.<p> Dependent on market factors such as the price of substitute feeds, exchange rates and transportation considerations, the magnitude of these savings could range between $7.29 and $0.34/tonne. The study recommends an understanding of these dynamics in order for livestock and ethanol producers to derive mutual benefits from the fledging biofuel industry in the Western plains.

In this study, the nutritional value of wheat distillers grains and the effect of further processing of these products on their nutritional value for rainbow trout were investigated in five experiments. In experiments 1 and 2, wheat distillers grains with solubles (WDDGS) was fractionated using grinding, sieving and elutriation sequentially. Apparent digestibility coefficients (ADC) of dry matter (DM), gross energy (GE), acid ether extract (AEE), ash and amino acids (AA) did not differ between the original WDDGS and the WDDGS protein concentrate (P > 0.05). However, the ADC of crude protein (CP) was significantly higher for WDDGS protein concentrate (88.0 %) than the original WDDGS (84.9 %) (P < 0.05). In experiments 3 and 4, the effect of aqueous fractionation on nutritional composition of wheat wet distillers grains (WWDG) from two local ethanol plants (plant 1 and plant 2) was evaluated. Aqueous fractionation increased levels of CP and GE in the processed WWDG from both plants. Fractionation significantly increased the ADC of DM, GE and AEE (P < 0.05). In contrast, protein digestibility was not influenced by the plant or the processing method (P > 0.10). In experiment 5, a 56 d growth trial was performed to determine the effect feeding the aqueous fractionated WWDG to rainbow trout on growth performance. Rainbow trout (n=22/ tank; body weight 136 g and 3 tanks/ treatment) were fed diets containing 0, 75, 150, 225 and 300 g kg-1 of the processed WWDG from plant 2. There were no significant linear or quadratic relationships between inclusion rate and specific growth rate (SGR), average daily gain (ADG) or feed to gain ratios (feed:gain). However, there was a significant negative linear relationship between inclusion rate and average daily feed intake (ADFI) (P < 0.05). The results of these studies suggest that both dry and aqueous fractionation are suitable methods to produce protein concentrates from wheat distillers grains but that the aqueous fractionation process was more effective in improving nutrient composition and increasing digestibility.

In this study, the nutritional value of wheat distillers grains and the effect of further processing of these products on their nutritional value for rainbow trout were investigated in five experiments. In experiments 1 and 2, wheat distillers grains with solubles (WDDGS) was fractionated using grinding, sieving and elutriation sequentially. Apparent digestibility coefficients (ADC) of dry matter (DM), gross energy (GE), acid ether extract (AEE), ash and amino acids (AA) did not differ between the original WDDGS and the WDDGS protein concentrate (P > 0.05). However, the ADC of crude protein (CP) was significantly higher for WDDGS protein concentrate (88.0 %) than the original WDDGS (84.9 %) (P < 0.05). In experiments 3 and 4, the effect of aqueous fractionation on nutritional composition of wheat wet distillers grains (WWDG) from two local ethanol plants (plant 1 and plant 2) was evaluated. Aqueous fractionation increased levels of CP and GE in the processed WWDG from both plants. Fractionation significantly increased the ADC of DM, GE and AEE (P < 0.05). In contrast, protein digestibility was not influenced by the plant or the processing method (P > 0.10). In experiment 5, a 56 d growth trial was performed to determine the effect feeding the aqueous fractionated WWDG to rainbow trout on growth performance. Rainbow trout (n=22/ tank; body weight 136 g and 3 tanks/ treatment) were fed diets containing 0, 75, 150, 225 and 300 g kg-1 of the processed WWDG from plant 2. There were no significant linear or quadratic relationships between inclusion rate and specific growth rate (SGR), average daily gain (ADG) or feed to gain ratios (feed:gain). However, there was a significant negative linear relationship between inclusion rate and average daily feed intake (ADFI) (P < 0.05). The results of these studies suggest that both dry and aqueous fractionation are suitable methods to produce protein concentrates from wheat distillers grains but that the aqueous fractionation process was more effective in improving nutrient composition and increasing digestibility.

The ethanol industry in Western Canada has seen significant growth in recent times spurred on mainly be environmental considerations. For a region with substantial grain production, increased prices from additional demand by the biofuel industry may inure to the benefit of grain farmers and land owners in the long term. The livestock industry however remains in a complex position facing the possibility of higher feed costs on the one hand and potential savings in feed cost on the other, with the availability of distillers grains- a by-product from ethanol production. The sectorial implications for the livestock sector could also be diverse and dependent upon the capacity to incorporate the distillers grains into the different feed rations. There is also the possibility of a spill-over effect from the US distillers grains market. This study therefore sought to complement current nutritional research by providing an economic perspective of the impact of distillers grains on the livestock industry in Western Canada. Focussing primarily on the beef cattle and hog industries, the study applied both linear programming and time-series techniques to assess potential benefits and costs. Potential positive economic benefits were observed for the inclusion of wheat and corn distillers grains with the former having a higher economic value in the high-protein feed segments.<p> Dependent on market factors such as the price of substitute feeds, exchange rates and transportation considerations, the magnitude of these savings could range between $7.29 and $0.34/tonne. The study recommends an understanding of these dynamics in order for livestock and ethanol producers to derive mutual benefits from the fledging biofuel industry in the Western plains.

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Nutrient profiles of distillers grains are primarily affected by the type of grain used for ethanol production, but the feeding value of triticale-based dry distillers grains plus solubles (TDDGS) has not been extensively studied. The first study showed that, compared to 20% corn- or wheat-based distillers grains diets, 20% TDDGS diet increased cis-9 trans-11 linoleic acid concentration in carcass fat without affecting growth performance of lambs. In the second study, lamb growth performance was not affected by the addition of up to 60% TDDGS in the diet, but the risk of urinary calculi increased at higher inclusion levels. Lambs fed 20% TDDGS had higher cold carcass weights and grade rules than lambs fed 40 or 60% TDDGS. Increasing TDDGS decreased diet digestibility, and increased nitrogen and phosphorus excretion. In conclusion, TDDGS can be used in diets for growing ruminants, and its utilization efficiency may be optimized at 20% dietary inclusion. / Animal Science