Thesis (MTech (Nature Conservation))--Cape Technikon, 2003. / The study was carried out on two adjacent farms on the plains of the Nama Karoo near Beaufort West. The impacts of three grazing treatments (a) zero grazing (b) non-selective grazing (c) conventional grazing, on plant diversity and certain vegetation parameters were compared. Unpredictable and variable rainfall and major disturbance events such as droughts drive vegetation change in the Nama Karoo. Major recruitment events are rare and can determine Karoo vegetation composition for many years. The diversity of plant species plays an important role in determining vegetation composition during major recruitment events and following drought or disturbance such as grazing. Grazing can influence the composition, abundance and seed production of Karoo plants and in so doing influence the future abundance of desirable and undesirable forage species. These changes may only become evident over long periods, but small changes in vegetation as a response to grazing treatment can accumulate considerably over time.On the farm Elandsfontein, studies have shown that non-selective grazing leads to a higher plant turnover rate, resulting in more vigorous and productive plants, and improved ecosystem functioning. However there is no evidence of this grazing system promoting or reducing plant diversity. The aim of this study was to test whether the non-selective grazing system promoted or reduced plant diversity compared to no grazing and conventional grazing. The hypothesis was that there were no differences between the grazing treatments in terms of plant diversity or any of the vegetation parameters measured. To evaluate this hypothesis, plant data were collected from the three grazing treatments using the Modified-Whittaker vegetation sampling method. The method was further modified for this study to allow for accurate abundance measurements rather than estimates, and an increased area for recording species richness. Using various diversity indices that incorporate species richness and the proportional abundance of species, plant diversity values for each treatment were obtained. No differences in terms of plant diversity were found between the treatments. A significant difference between treatments was found in the density of plants, particularly in perennial grasses and shrubs. Canopy cover percentage did not differ for individual species or as total cover between the treatments.

Quartz fields contribute significantly to plant diversity in the Succulent Karoo biome. They are distinctly different from surrounding habitats and have high levels of plant endemism. Biological soil crusts are features of quartz field soils and fulfill a vital function in that they stabilize soils. It is important for managers of nature reserves and agricultural rangelands to know what factors influence quartz field soils and vegetation. Both stakeholders could benefit from new information that would allow for informed decision-making regarding land-use on quartz fields. The present study took place in the Namaqua National Park that contains a significant proportion of the Riethuis-Wallekraal quartz fields phytochorion. The first part of the study aimed to understand whether certain land-use activities potentially destabilize quartz field soils, which might have possible ramifications for associated biological soil crusts and vegetation. It was followed by relating variation in soil stability with species richness, cover and species composition of quartz field vegetation. Overall, positions assumed to be impacted by land-use activities had less stable soils compared to positions assumed not be impacted. Soil stability had a significant influence on species richness and cover but to a lesser degree on species composition. Quartz field vegetation was significantly influenced by soil physical and chemical properties as well as location in the quartz fields landscape. The second part of the study aimed at understanding how species richness of isolated quartz outcrops is related to their size compared to that of a mainland body of quartz outcrops. No clear species-area relationships emerged from the study. There were significant differences between isolated outcrops and mainland outcrops in substrate and vegetation composition. Findings are discussed in relation to Island Biogeography Theory.

The responsiveness of South African fauna to climate change events is poorly documented and not routinely incorporated into regional conservation planning exercises. The lack of detailed distribution data for appreciable numbers of taxa demands a modeling solution. We develop a climate envelope model to predict potential distribution range changes. The model can be used to interpolate the distributions of poorly sampled taxa as well as predict responses to a changing climate. It is predicted that species from the more arid western parts of South Africa will be subject to severe range contraction and range shifts whereas the species from the more mesic eastern parts will experience range contraction with limited range shift. Species that could act as climate change indicator taxa are identified based on their predicted extreme range change responses to climate change. Red-data and vulnerable species were more likely to display range change than less threatened species. Without mitigatory action, conservation areas are likely to lose species. The likelihood of successful range shifts will be affected by the nature of novel communities, habitat suitability and the degree of land transformation encountered. Given the extent of the predicted spatial responses, conservation planners can no longer afford to ignore future climate impacts on species distribution patterns. Disease risk profiles are also expected to change with climate; currently, susceptible forestry plantations exist in areas which may be invaded by an economically important pathogen. Resistant clones should be planted in these future high-risk areas. A decrease in precipitation is an important feature of a future climate. This decrease is expected to impact on the agricultural sector by reducing total employment as producers switch to a more extensive production pattern. The total decline in welfare, therefore, will fall disproportionately on the poor. Climate change presents a significant treat to the South African biodiversity estate, and our ability to manage this transition in the face of changing and competing land uses. Adaptation and mitigation options do exist but they are hampered by a lack of definitive analyses, and ultimately, political will to prioritise the threat of climate change. / Thesis (PhD(Zoology))--University of Pretoria, 2006. / Zoology and Entomology / unrestricted

Thesis (MSc (Microbiology))--Stellenbosch University, 2008. / The soil environment is thought to contain a lot of the earth’s undiscovered biodiversity. The aim of this study was to understand the extent of microbial diversity in the unique ecosystem of the Western Cape’s fynbos biome. It is known that many processes give rise to this immense microbial diversity in soil. In addition the aim was to link microbial diversity with the soils physio-chemical properties as well as the plant community’s structure. Molecular methods especially automated ribosomal intergenic spacer analysis (ARISA) was used in the study. The most important property of environmental DNA intended for molecular ecology studies and other downstream applications is purity from humic acids and phenolic compounds. These compounds act as PCR inhibitors and need to be removed during the DNA extraction protocol. The fist goal in the study was to develop an effective DNA extraction protocol by using cationic locculation of humic acids. The combination of cationic flocculation with CuCl2 and the addition of PVPP and KCl resulted in a high yield of DNA, suitable for PCR amplification with bacterial and fungal specific primers. Determining the reproducibility and accuracy of ARISA and ARISA-PCR was important because these factors have an important influence on the results and effectiveness of these techniques. Primer sets for automated ribosomal intergenic spacer analysis, ITS4/ITS5, were assessed for the characterization of the fungal communities in the fynbos soil. The primer set delivered reproducible ARISA profiles for the fungal community composition with little variation observed between ARISAPCR’s. ARISA proved useful for the assessment and comparison of fungal diversity in ecological samples. The soil community composition of both fungal and bacterial groups in the Sand fynbos was characterized. Soil from 4 different Sand fynbos sites was compared to investigate diversity of eubacterial and fungal groups at the local as well as a the landscape scale. A molecular approach was used for the isolation of total soil genetic DNA. The 16S-23S intergenic spacer region from the bacterial rRNA operon was amplified when performing bacterial ARISA from total soil community DNA (BARISA). Correspondingly, the internal transcribed spacers, ITS1, ITS2 and the 5.8S rRNA gene from the fungal rRNA operon were amplified when undertaking fungal ARISA (F-ARISA). The community structure from different samples and sites were statistically analysed. ARISA data was used to evaluate different species accumulation and estimation models for fungal and bacterial communities and to predict the total community richness. Diversity, evenness and dominance were the microbial communities were used to describe the extent of microbial iversity of the fynbos soils. The spatial ordination of the bacterial and fungal species richness and diversity was considered by determining the species area relationship and beta diversity of both communities. The correlation between the soil physio-chemical properties was determined. The plant community structure data was correlated with the fungal and the bacterial community structure. The results indicated that bacterial species numbers and diversity were continually higher at the local scale. Fungi however showed higher species turnover at the landscape scale. Bacterial community structure showed stronger links to the plant community structure whereas the fungi community structure conformed to spatial separation patterns. To further investigate the diversity of soil microbes the potential of genus specific primes was investigated. The genus Penicillium is widespread in the soil environment and the extent of its diversity and distribution is however not. For this reason Penicillium was chosen as a model organism. To expand the insight into the diversity of Penicillium species in the fynbos soil ecosystem, a rapid group specific molecular approach would be useful. Penicillium specific primers targeting the 18S rRNA ITS gene region were evaluated. Fungal specific primers ITS4 and ITS5, targeting the internal transcribed region (ITS) were used to target Penicillium specific in the soil sample. Nested PCR, using primer Pen-10 and ITS5, was then utilized to target Penicillium species specifically. The discrimination of Penicillium species was possible due to length heterogeneity of this gene region. Eight different peaks was detected in the soil sample with ARISA and eight different species could be isolated on growth media. The technique proved useful for the detection and quantification of Penicillium species in the soil.

M.Sc. (Aquatic Health) / The relationships between water quality variables and phytoplankton diversity in the Hartbeespoort Dam were assessed spatially and temporally from February 2011 to March 2012 to evaluate the effects of the water quality variables on cyanobacterial bloom (Microcystis aeruginosa) hence aquatic macrophytes growth (Eichhornia crassipes) in the dam. Variables measured using standard methods included; temperature, pH, electrical conductivity, total suspended solids, dissolved oxygen, nitrate, nitrite, total phosphorous , ammonium, trace metals, chlorophyll-a and the phytoplankton community. The physical parameters ranged between: temperature (11.8-28 oC), electrical conductivity (282-796 ƒÊS/cm), dissolved oxygen (0.33-32.2 mg/L), pH (6.95-9.91) and total suspended solids (2-372 mg/L). Chemical variables ranged between; total phosphorous (0.02-3.5 mg/L), nitrate (0.03-21.2 mg/L), nitrite (0.02-0.48 mg/L) and ammonium (0.01-1.58 mg/L), chlorophyll-a (0.13-8693 ƒÊg/L), and exceed the TWQR values of the South African Water Quality Guidelines for aquatic ecosystem health health. Metal concentrations in water had the following decreasing order; macro elements: potassium > calcium >sodium > magnesium. Microelements: iron >zinc > aluminium > copper > nickel > manganese > chromium> selenium > lead > silver > arsenic > cadmium. Iron had the highest concentration among microelement of 631.62 ƒÊg/L and potassium the highest concentration amongst macro element of 34.49 mg/L. Six Different algal divisions were found in the dam with cyanophyta (cyanobacteria) been the most dominant group (95 %) and M.aeruginosa the most dominant species (69 %). The current study revealed an increase in physical parameters, chlorophyll-a and phytoplankton community and a decrease in chemical parameters in the summer months. An inverse relationship was observed in the winter months at all sites. One-way ANOVA showed a significant differences for physical variables (p <0.05) between months, with no significant differences noted (p > 0.05) between sites and between depths. Chemical variables however, showed a significant differences between months, sites and between depths (p <0.05). A 2-tailed Pearson correlation revealed negative correlations between temperatures and phosphorus, ammonia, nitrate, nitrite, electrical conductivity and iron (r=-0.298;-0.232;-0.099;-0.461;-0.441;-0.260) respectively and positive correlations between temperatures and chlorophyll-a and pH (r= 0.240; 0.609 ;) respectively (p <0.05; p <0.01). Canonical discriminant functions analysis revealed similarities and dissimilarities in water quality variables temporally and spatially with eigenvalues of 84.6 % and 59.1 % respectively. There was an adverse impact of the physico-chemical variables on the phytoplankton community, therefore aquatic macrophytes growth in the dam. The current study revealed that temperature, pH, phosphorous, nitrate and probably iron, copper, zinc and selenium may have contributed to the hypertrophic state of the dam, hence cyanobacterial bloom and growth of aquatic macrophytes.

Please read the abstract in the section 00front of this document / Thesis (PhD (Microbiology))--University of Pretoria, 2006. / Microbiology and Plant Pathology / unrestricted

The aim of this study is to gain a predictive understanding of the patterns and determinants of plant biodiversity in temperate, mesic grasslands of South Africa with a primary focus on the geographical area of the Eastern Cape. From a review of the literature on hypotheses explaining diversity (Chapter 2) it was possible to formulate a number of hypotheses that could be tested to explain species richness patterns in Eastern Cape grasslands. This thesis is organised so that each main chapter deals with a specific body of theory concerning the explanation of diversity patterns. A detailed description of the study area is provided (Chapter 3), including environmental variation and a description of major vegetation patterns. A summary is provided of grassland plant community patterns, as determined by phytosociological studies in the study area. A multivariate analysis of environmental variables was undertaken to determine which variables contributed the most towards explaining environmental variation in the study area and to determine whether any variables co-vary, a possible problem for any multivariate analysis in later chapters. Altitude produced one of the strongest gradients in the study area. There were a number of variables that were correlated with altitude, most notably temperature. Rainfall co-varied partially with altitude, but there was also a strong rainfall gradient perpendicular to the altitude gradient. A description of species richness, diversity and evenness patterns at the plot scale within different grassland plant communities of the Eastern Cape is provided in Chapter 4. To determine whether the environment acts differently on different growth forms, the contribution to species richness by different major growth forms is analysed. Furthermore, since the majority of literature attempts to explain diversity in terms of environmental factors, it was necessary to analyse the relationship between species richness and various environmental variables. The results indicate that there is high variation in species richness both within and among grassland communities. Forbs make the most significant contribution to overall species richness per 100 m2, followed by grasses. Variance in richness of all species together is not significantly related to environmental variables in mesic grasslands, but is significantly related to environmental variables in semi-arid grasslands. The result of greatest interest from this chapter is the fact that richness amongst different life-forms in the same place is explained by different environmental factors, indicating that the environmental factors that affect coexistence of species have a different effect on different life-forms. A classification of all the species of the dataset into plant functional types using a multivariate approach based on functional traits was conducted (Chapter 5). The grass species were classified into 16 functional types and the forbs into 14 functional types. The functional type classification provided the opportunity for undertaking analyses to develop an understanding of 8 the contribution by niche differentiation towards promoting species richness (Chapter 6). The results provide evidence of niche differentiation in the grasslands of the study area and also that niche differentiation promotes species richness in the grasslands of the study area. It was found that higher rainfall grasslands are less structured by niche differentiation than semi-arid grasslands. A regional / historical analysis is undertaken (Chapter 7) to investigate the relationship between the regional species pool and local richness, and the relationship between local richness and phytochorological diversity. Regional richness appears to have little effect in promoting local richness in grassland plant communities of the study area except at sites where there is high local richness. This provides an indication that regional richness only promotes local richness in the absence of local limiting factors. Phytochorological diversity promotes local richness, but mostly through diversity amongst species with narrow distribution ranges. Some theories ascertain that seasonal uncertainty may provide opportunities to species that would otherwise be outcompeted and thereby promote local richness. The degree to which seasonal uncertainty and seasonality promote local richness in the Eastern Cape grasslands was therefore investigated (Chapter 8). A weak relationship exists between these variables and local species richness in grassland communities of the study area, indicating that they do not promote niche differentiation to a significant degree in the study area. It is clear that in the grasslands of the Eastern Cape, environmental limiting factors are more important in semi-arid grasslands and species interactions are more important in mesic grasslands for structuring plant communities (Chapter 9, Discussion). Regional processes do not appear to be important in structuring local communities, but the analysis undertaken in this study shows that they may be significant when factors operating at the other two levels are overcome (species interactions and environmental limiting factors.

Thesis (PhD(Agric) (Conservation Ecology and Entomology))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: See full text document for abstract / AFRIKAANSE OPSOMMING: sien volteks dokument vir die opsomming

Thesis (MTech (Applied Sciences))--Cape Peninsula University of Technology, 2007 / Changes in plant specIes richness and diversity were investigated in Rocherpan Nature Reserve across a fence (or old fence line) separating 34 years of conservation management, conservation management for 11 years south and north, natural veld grazed by cattle and goats, natural veld grazed by sheep, and strip-ploughed veld grazed by sheep. Vegetation surveys were conducted in September 2001. The modified 20 m x 50 m Whittaker plot design with its attractive features of long-thin plots and the original Whittaker plot design were used. Number of species (as richness data) and the numbers of individual recordings per species per land use (as abundance data) were used in calculations in the 20 m x 50 m (1000 m2 ) plots. The species richness index showed significantly smaller numbers of species in the conservation management 11 years south land. The results also showed an increase in species numbers under the grazing land use systems. The data from the comparative study of the five different species diversity indices showed no significant differences. As a result, the Shannon-Wiener index was selected for further assessing the species diversity index and the species diversity significance of different land uses in the West Coast Strandveld. In this particular study, the species that showed an increase in vegetation cover in response to grazing land use regimes were Hemimeris racemosa (annual herb) and Ehrharta brevifolia (annual grass).

Text in Afrikaans, abstract in Afrikaans and English / Lineere plantegroeipatrone bestaande uit afwisselende grasryke mikrotrog- en boomryke- kruinbane wat parallel met die helling georienteer is, kom voor op die voetbang van die Baviaansberg, in die Warmbadgebied, Noordelike Provinsie. Die hoofdoelwitte van die studie was om uit 'n landskapekologiese benadering die verskynsel te probeer verklaar, om die vertikale en horisontale variasie van eienskappe te kwantifiseer en om die impak van die mens daarop te benadruk. Die differensiasie in die Tukulu- en Etoshagrondvorms in onderskeidelik mikrotroe en -kruine is die gevolg van grondprosesse soos illuviasie van klei, loging en ferrolise.Granulometriese analise dui daarop dat die moedermateriaal van gronde van een bron afkomstig is. Kolluviasie en afsetting van moedermateriaal op die voethang was waarskynlik die beginpunt van landskapontwikkeling waar verdeelde oppervlakvloei van water oenskynlik die mikrostrukture geinisieer het. Na aanleiding hiervan het die ontwikkeling van grondverskille en waterregimeverskille tot plantegroeiverskille gelei. Faktore wat die patroon geaksentueer het is termietaktiwiteite, brand en beweiding. / A linear vegetation pattern consists of alternating grassland micro troughs and woody micro crests lanes which were orientated parallel to the slope occurred on the foot slope of the Baviaansberg, in the Warmbaths area, Northern Province. From a landscape ecological approach the principal aims of the study was to explain the phenomenon, to quantify the vertical and horizontal variation of features and to accent the impact of man on it. The differentiation in Tukulu and Etosha forms in micro troughs and micro crests respectively were the effect of several soil processes. Granulometric analyses showed that the parent material of soil was derived from one source. Colluviation and deposit of parent material was probably the starting-point of landscape development where the divided surface flow of water apparently initiated the micro structures. As a result of this the development of soil and water regime differences lead to vegetation differences. / Geography / M. Sc. (Geografie)