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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Investigations of brain perturbation by magnetic resonance spectroscopy

Paramananthan, Navaneethan January 1995 (has links)
No description available.
2

Regulation of Satellite Cell Homeostasis by C/EBPβ: Therapeutic Perspectives

Lala-Tabbert, Neena January 2016 (has links)
Regeneration of adult skeletal muscle relies upon a population of quiescent myogenic progenitor cells, called satellite cells (SCs). Upon injury, SCs activate, proliferate, differentiate and fuse to make new myofibers or to repair damaged ones. SCs can also self-renew to repopulate the SC niche. The balance between differentiation and self-renewal is critical to maintain muscle homeostasis and changes in this equilibrium can lead to chronic muscle degeneration. For example, Duchenne’s muscular dystrophy (DMD) is characterized by rounds of muscle degeneration and regeneration leading to increased muscle wasting. One approach to treat DMD is transplantation of SCs. For this treatment to be viable, transplanted cells must contribute to repairing injured muscle and repopulating the SC niche. Here, we show that the transcription factor CCAAT/Enhancer Binding Protein beta (C/EBPβ) regulates SC function. C/EBPβ is down-regulated during differentiation and persistent expression of C/EBPβ inhibits differentiation and expression of the myogenic regulatory factors MyoD and Myogenin. C/EBPβ also promotes Pax7 expression by directly binding to and regulating Pax7 transcription. Using genetic tools to conditionally excise C/EBPβ expression in SCs, we found that C/EBPβ-null SCs lose quiescence and precociously differentiate at the expense of self-renewal. After a single injury, C/EBPβ-deficient SCs failed to self-renew, resulting in impaired muscle repair after a second injury. C/EBPβ-induced quiescence also requires upregulation of caveolin-1. Furthermore, pharmacological manipulation of C/EBPβ expression with the phosphodiesterase inhibitor, isobutylmethylxanthine (IBMX), increased the number of cells available for transplantation into dystrophic muscle and enhanced the expression of stem cell markers in a C/EBPβ-dependent fashion. IBMX treatment improved cell survival and migration, engraftment into the SC niche and repair of dystrophic muscle. Together, these results demonstrate that C/EBPβ is an important regulator of SC function and that pharmacological manipulation of C/EBPβ improves culture conditions for the expansion and selection of SCs available for cell therapy for the treatment of muscular dystrophies.
3

Kinase Domain Receptor Is a Modulator of Satellite Stem Cell Asymmetric Division

Chen, William 24 March 2021 (has links)
The regulation of muscle stem cell (MuSC) asymmetric division plays an essential role in controlling the growth and repair of skeletal muscle. Perturbations in MuSC function have been demonstrated in disease and aging contexts such as Duchenne’s Muscular Dystrophy (DMD) and sarcopenia. We developed and optimized a high content analysis platform combining lineage tracing, myofiber culture, imaging, and bioinformatic analysis to determine modulators of muscle stem cell division. We discover kinase domain receptor (KDR) as a positive modulator of MuSC asymmetric division and confirmed its expression in satellite cells by ddPCR and immunofluorescence. Knockdown of KDR significantly reduces the numbers of asymmetric divisions, whereas ligand stimulation of KDR increases the numbers of asymmetric divisions. KDR signaling is impaired in dystrophin- deficient satellite cells and requires a polarized cell environment established by the dystrophin glycoprotein complex (DGC) to direct asymmetric division. Mice lacking KDR in MuSCs exhibit reduced numbers of satellite cells due to precocious differentiation, and deficits in regeneration consistent with impaired asymmetric division and reduced generation of progenitors. Therefore, our experiments identify KDR signaling as playing an essential role in MuSC function in muscle regeneration. These findings further our understanding of muscle stem cell biology, and in particular, the role of asymmetric division under homeostatic and regenerative conditions.

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