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1	Utilização de marcadores de rDNA-PCR e tDNA-PCR para tipagem de isolados clínicos de Pseudomonas aeruginosa SPACOV, Isabel Cristina Guerra January 2005 (has links) Made available in DSpace on 2014-06-12T18:06:32Z (GMT). No. of bitstreams: 2 arquivo6313_1.pdf: 1481990 bytes, checksum: 994600fb8e4272b6f803e8477c45a54d (MD5) license.txt: 1748 bytes, checksum: 8a4605be74aa9ea9d79846c1fba20a33 (MD5) Previous issue date: 2005 / Pseudomonas aeruginosa é uma bacteria Gram-negativa ubíqua e oportunista. Na rotina hospitalar, os marcadores fenotípicos nem sempre revelam a diversidade das bactérias distribuídas nos diversos setores, assim, aplicamos três métodos moleculares baseados na amplificação por PCR do locus de rDNA e tDNA para caracterizar a diversidade genética de linhagens de P. aeruginosa isoladas em um hospital público em Recife-PE, Brasil. O rDNA-PCR detectou 15% de variabilidade genética, contra 23% do tDNA-PCR e 23% do Duplex-PCR. O setor com maior diversidade genética foi a Unidade de Tratamento Intensivo do hospital, o qual apresentou quatro genótipos bacterianos diferentes. A ocorrência de linhagens de P. aeruginosa pertencentes ao mesmo genótipo e mesmo perfil de resistência a múltiplas drogas (MDR), em diferentes setores do hospital, sugere que há infecção cruzada entre pacientes. Os dados apresentados pelo rDNA-PCR, tDNA-PCR e Duplex-PCR, em associação ao perfil de susceptibilidade antimicrobiana provêem valiosas informações epidemiológicas para o controle de infecções hospitalares causadas por P. aeruginosa P. aeruginosa Tipagem molecular rDNA-PCR tDNA-PCR Duplex-PCR
2	Establishment and application of real-time PCR-based methods to study the epidemiology of Fusarium Head Blight / Etablierung und Anwendung der Real-time PCR für epidemiologische Untersuchungen zu Ährenfusariosen Brandfaß, Christoph 13 July 2006 (has links) No description available. 630 Landwirtschaft, Veterinärmedizin YEK 181 Agricultural Sciences Methode Mais DNA DON Duplex PCR Partielle Weißährigkeit FHB Fusarium culmorum Fusarium graminearum Gibberella zeae Maisrückstände Schmelzkurvenanalyse Mykotoxine Pflanzenmaterial Quantifizierung Ährenspindeln Getreide Weizen assay corn DNA DON duplex PCR ear blight FHB Fusarium culmorum Fusarium graminearum Gibberella zeae maize debris melting curve analysis mycotoxins plant material quantification rachides small-grain cereals wheat scab 48.54
3	Stanovení autenticity potravin rostlinného původu pomocí molekulárních metod / Determination of athenticity of plant foods by molecular techniques Plášková, Anna January 2020 (has links) The aim of presented diploma thesis was to determination of authenticity of fruit baby foods for early infant feeding using molecular methods. In the experimental part, isolation kit was used for isolation of plant DNA from fruits (strawberry, apricot, raspberry, apple) and from six commercial fruit products for children. Isolated DNA was characterized and verified using PCR methods with primers specific for plant rDNA (ITS2). Specific primer pairs were designed to amplify DNA for the detection of one fruit species. Primer specificity was assessed with four fruit species. A mixture of fruit puree from the two fruits was used to determine the sensitivity of the multiplex PCR assay. Six commercial fruit products were evaluated to verify the applicability of the multiplex PCR assay. The methodology of molecular detection of fruit DNA by qPCR and multiplex qPCR (duplex) includes approaches, which enable to detect two fruits (strawberry-raspberry, apricot-apple) in one reaction and thus reduces time and money requirements.

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