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1	A Novel Biosensing Interface Preparation Method for ElectroMagnetic Piezoelectric Acoustic Sensor Sheng, Jack 06 April 2010 (has links) Preliminary work towards the development of novel biosensing interfaces for EMPAS (ElectroMagnetic Piezoelectric Acoustic Sensor) is presented in this manuscript. This method involves the use of unprecedented thiosulfonate-based linkers to construct robust and durable SAMs (Self-Assembling Monolayer) onto piezoelectric quartz crystals, which can chemoselectively immobilize thiol-containing biomolecules under aqueous conditions in a single, straightforward, reliable and coupling-free manner. Initial efforts are devoted to the construction of SAMs and the subsequent immobilization of thiol-containing biomolecules, and then characterization by CAMs (Contact Angle Measurement) and ARXPS (Angle-Resolved X-ray Photoelectron Spectroscopy). This method is then implemented into the construction of biosensing interfaces dedicated to the detection of avidin. With the incorporation of OEG (Oligo(Ethylene Glycol)) backbone and diluent in the method, 14-fold difference in signal response of EMPAS was observed between biotinylated and unfunctionalized SAMs. biosensor self-assembling monolayer EMPAS thiolsulfonate 0486
2	A Novel Biosensing Interface Preparation Method for ElectroMagnetic Piezoelectric Acoustic Sensor Sheng, Jack 06 April 2010 (has links) Preliminary work towards the development of novel biosensing interfaces for EMPAS (ElectroMagnetic Piezoelectric Acoustic Sensor) is presented in this manuscript. This method involves the use of unprecedented thiosulfonate-based linkers to construct robust and durable SAMs (Self-Assembling Monolayer) onto piezoelectric quartz crystals, which can chemoselectively immobilize thiol-containing biomolecules under aqueous conditions in a single, straightforward, reliable and coupling-free manner. Initial efforts are devoted to the construction of SAMs and the subsequent immobilization of thiol-containing biomolecules, and then characterization by CAMs (Contact Angle Measurement) and ARXPS (Angle-Resolved X-ray Photoelectron Spectroscopy). This method is then implemented into the construction of biosensing interfaces dedicated to the detection of avidin. With the incorporation of OEG (Oligo(Ethylene Glycol)) backbone and diluent in the method, 14-fold difference in signal response of EMPAS was observed between biotinylated and unfunctionalized SAMs. biosensor self-assembling monolayer EMPAS thiolsulfonate 0486
3	Label-free, Direct Detection of Cocaine using an Aptamer in Conjunction with an Ultra-high Frequency Acoustic Wave Sensor Bokhari, Syed Sumra 11 August 2011 (has links) This study embarks on exploiting the Thickness Shear Mode (TSM) acoustic wave sensor and the ElectroMagnetic Piezoelectric Acoustic Sensor (EMPAS) towards the study of aptamer-to-cocaine binding in a label-free direct approach. The high sensitivity and selectivity offered by the EMPAS in combination with alkyltrichlorosilane-based self-assembled monolayers proved superior towards the detection of cocaine. The most efficient method for the attachment of the aptamers onto the sensor surface to construct highly dense populations of the aptamer molecules with retained biomolecule activity is shown to be dependent on the composition of immobilizing solution and on the amount of spacing provided in the plane of the aptamer molecules. The distinct ligand-induced binding mechanisms and regeneration capabilities of the two anti-cocaine aptamers are monitored with the EMPAS. Utilizing this sensor to monitor cocaine-aptamer interactions will serve as the first piezoelectric aptasensor for the detection of a small molecule. acoustic biomedical engineering cocaine piezoelectric biosensor self assembled monolayers thickness shear mode anti-cocaine aptamer aptasensor TSM EMPAS aptamer immobilization optimization label-free cocaine detection 0486
4	Label-free, Direct Detection of Cocaine using an Aptamer in Conjunction with an Ultra-high Frequency Acoustic Wave Sensor Bokhari, Syed Sumra 11 August 2011 (has links) This study embarks on exploiting the Thickness Shear Mode (TSM) acoustic wave sensor and the ElectroMagnetic Piezoelectric Acoustic Sensor (EMPAS) towards the study of aptamer-to-cocaine binding in a label-free direct approach. The high sensitivity and selectivity offered by the EMPAS in combination with alkyltrichlorosilane-based self-assembled monolayers proved superior towards the detection of cocaine. The most efficient method for the attachment of the aptamers onto the sensor surface to construct highly dense populations of the aptamer molecules with retained biomolecule activity is shown to be dependent on the composition of immobilizing solution and on the amount of spacing provided in the plane of the aptamer molecules. The distinct ligand-induced binding mechanisms and regeneration capabilities of the two anti-cocaine aptamers are monitored with the EMPAS. Utilizing this sensor to monitor cocaine-aptamer interactions will serve as the first piezoelectric aptasensor for the detection of a small molecule. acoustic biomedical engineering cocaine piezoelectric biosensor self assembled monolayers thickness shear mode anti-cocaine aptamer aptasensor TSM EMPAS aptamer immobilization optimization label-free cocaine detection 0486

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