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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
provided by the
Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
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Label-free, Direct Detection of Cocaine using an Aptamer in Conjunction with an Ultra-high Frequency Acoustic Wave SensorBokhari, Syed Sumra 11 August 2011 (has links)
This study embarks on exploiting the Thickness Shear Mode (TSM) acoustic wave sensor and the ElectroMagnetic Piezoelectric Acoustic Sensor (EMPAS) towards the study of aptamer-to-cocaine binding in a label-free direct approach. The high sensitivity and selectivity offered by the EMPAS in combination with alkyltrichlorosilane-based self-assembled monolayers proved superior towards the detection of cocaine. The most efficient method for the attachment of the aptamers onto the sensor surface to construct highly dense populations of the aptamer molecules with retained biomolecule activity is shown to be dependent on the composition of immobilizing solution and on the amount of spacing provided in the plane of the aptamer molecules. The distinct ligand-induced binding mechanisms and regeneration capabilities of the two anti-cocaine aptamers are monitored with the EMPAS. Utilizing this sensor to monitor cocaine-aptamer interactions will serve as the first piezoelectric aptasensor for the detection of a small molecule.
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Label-free, Direct Detection of Cocaine using an Aptamer in Conjunction with an Ultra-high Frequency Acoustic Wave SensorBokhari, Syed Sumra 11 August 2011 (has links)
This study embarks on exploiting the Thickness Shear Mode (TSM) acoustic wave sensor and the ElectroMagnetic Piezoelectric Acoustic Sensor (EMPAS) towards the study of aptamer-to-cocaine binding in a label-free direct approach. The high sensitivity and selectivity offered by the EMPAS in combination with alkyltrichlorosilane-based self-assembled monolayers proved superior towards the detection of cocaine. The most efficient method for the attachment of the aptamers onto the sensor surface to construct highly dense populations of the aptamer molecules with retained biomolecule activity is shown to be dependent on the composition of immobilizing solution and on the amount of spacing provided in the plane of the aptamer molecules. The distinct ligand-induced binding mechanisms and regeneration capabilities of the two anti-cocaine aptamers are monitored with the EMPAS. Utilizing this sensor to monitor cocaine-aptamer interactions will serve as the first piezoelectric aptasensor for the detection of a small molecule.
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