Presenta un estudio de la lengua y sociedad Ese Eja que se enmarca dentro del modelo de la lingüística cognitiva. Determina que los conocimientos tradicionales referidos a los peces se reflejan a través de los nombres que se les ha asignado y que estos evidencian una taxonomía popular de los peces. Registra el conocimiento tradicional sobre los peces que se manifiesta en cuentos, canciones y relatos de tradición oral.

El presente trabajo de investigación es de tipo descriptivo, el cual está centrado en la descripción y explicación de los valores y funciones semánticas de Mahoya en la estructura textual de la lengua Ese Eja. Por ello, una de las tareas es determinar como Mahoya se establece dentro de la estructura de los textos (de tradición oral), la conexión entre párrafos y cuál o cuáles son los contextos en los cuales aparece. Los datos han sido los siguientes trabajos de investigación y trabajo realizado por los Misioneros Dominicos: ÁLVAREZ Fernández, José. OP.(Misionero Dominico), AZA, José, Pio. O.P. (Misionero Dominico), algunas de las frases en Ese Eja, han sido recopiladas del trabajo realizado por Jack y Nola Shoemaker con Mildred. L. Larson (ILV) en Relaciones Comunicacionales en la Gramática Ese Ejja (1983). Algunos de los mitos han sido tomados como referencia del trabajo realizado por la lingüista Dra. María Chavarría M. (UNMSM) en; Con la Voz de Nuestros Viejos Antiguos, Eséha Echíikiana Esoiho (1984). A la vez, se han utilizado los mitos recopilados y datos del trabajo de campo realizado en la comunidad Ese Eja de Palma Real (Madre de Dios) en los años 2005 y 2013 respectivamente.

This research aimed at developing a research framework for the emerging field of enterprise systems engineering (ESE). The framework consists of an ESE definition, an ESE classification scheme, and an ESE process. This study views an enterprise as a system that creates value for its customers. Thus, developing the framework made use of system theory and IDEF methodologies. This study defined ESE as an engineering discipline that develops and applies systems theory and engineering techniques to specification, analysis, design, and implementation of an enterprise for its life cycle. The proposed ESE classification scheme breaks down an enterprise system into four elements. They are work, resources, decision, and information. Each enterprise element is specified with four system facets: strategy, competency, capacity, and structure. Each element-facet combination is subject to the engineering process of specification, analysis, design, and implementation, to achieve its pre-specified performance with respect to cost, time, quality, and benefit to the enterprise. This framework is intended for identifying research voids in the ESE discipline. It also helps to apply engineering and systems tools to this emerging field. It harnesses the relationships among various enterprise aspects and bridges the gap between engineering and management practices in an enterprise. The proposed ESE process is generic. It consists of a hierarchy of engineering activities presented in an IDEF0 model. Each activity is defined with its input, output, constraints, and mechanisms. The output of an ESE effort can be a partial or whole enterprise system design for its physical, managerial, and/or informational layers. The proposed ESE process is applicable to a new enterprise system design or an engineering change in an existing system. The long-term goal of this study aims at development of a scientific foundation for ESE research and development.

Low density lipoprotein receptor (LDLR) is an apolipoprotein E (apoE) receptor and may play a role in Alzheimer’s disease (AD) development. A single nucleotide polymorphism (SNP), rs688, that has been identified to modulate the splicing efficiency of LDLR exon 12 and is associated with higher cholesterol and AD in some case-control populations. The exon 12 deleted mRNA is predicted to produce a soluble form of LDLR that fails to mediate apoE uptake. To gain additional insights, in this study, I seek to understand the regulation of LDLR splicing efficiency. To identify functional cis-elements within LDLR exon 12, I mutated several conserved putative exonic splicing enhancers (ESEs) to neutralize their affinity to serine/arginine-rich (SR) proteins. Transfection of wild type (WT) or mutant LDLR minigenes in HepG2 cells was performed, and splicing efficiency evaluated by quantitative RT-PCR. The results showed that two functional ESEs within exon 12, near rs688, are critical to LDLR splicing. To identify splicing factors that modulate exon 12 splicing, I co-transfected an LDLR minigene and vectors encoding different SR proteins and heterogeneous nuclear ribonucleoproteins (hnRNPs). After quantifying the splicing efficiency, I found that SRp20 and SRp38 increased exon 11- skipping. Moreover, ectopic expression of SRp38-2 and hnRNP G increased exon 11&12-skipping. Interestingly, the actions of hnRNP G did not require its RNA recognition motif (RRM). To further investigate the role of theses splicing factors on LDLR splicing, I quantified the expression level of these splicing factors as well as LDLR splicing efficiency in human brain and liver. I found that SRp38 mRNA expression is associated with LDLR splicing efficiency. In conclusion, this study discovered that rs688 is located close to the two functional ESEs within LDLR exon 12, and revealed a role of SRp38 in LDLR splicing efficiency.

La presente tesis aborda el análisis del acento nominal en la lengua ese eja de la familia Takana. Esta es una lengua muy poco documentada que se encuentra en peligro de extinción, lo cual justifica su documentación con fines patrimoniales además de académicos. La finalidad de esta tesis es describir apropiadamente las características del acento en la palabra nominal del ese eja y describir los parámetros que rigen su cómputo dentro del marco de la teoría métrica autosegmental. Para ello, aplica encuestas y grabaciones que brindan la evidencia necesaria para el análisis que contrasta los parámetros propuestos por la teoría para este fin. El principal resultado de esta tesis es la demostración de que el ese eja es una lengua que basa su cómputo acentual en la mora, y que esta unidad es pertinente tanto para la construcción de pies trocaicos como para la aplicación de la extrametricalidad, ambos fenómenos muy escasos en las lenguas documentadas del mundo. Nuestra propuesta muestra al ese eja como una lengua muy regular en términos de acento nominal, en la cual las escasísimas “irregularidades” halladas no son tales a la luz de nuestra propuesta: la mora como unidad de análisis. / Tesis

Nous tentons dans cette thèse d?interpréter les Extreme Scattering Events (ESE?s) par la turbulence naturelle du milieu interstellaire (MIS) ionisé. Il est bien connu que les inhomogénéités du MIS ionisé suivent un spectre de puissance de Kolmogorov. Dans l?approximation de l?écran mince, on montre d?abord en optique géométrique qu?on peut produire le phénomène d?ESE. On effectue ensuite le calcul dans le cadre de la diffraction de Fresnel. On obtient des ?pseudo?-ESEs qui ne sont pas très profonds et n?apparaissent pas au même moment aux trois fréquences simulées comme dans le cas des observations du pulsar B1937+21 au radiotélescope de Nançay. On montre par contre que les paramètres obtenus de la scintillation simulée sont en très bon accord avec les observations et la théorie. Cette étude conduite sur un ?ALPHA server? est une première étape nécessaire et importante dans l?étude de la scintillation interstellaire.

Human epithelium-specific ETS transcription factor-1 (ESE-1), which is also known as E74-like factor-3 (Elf3) in mice, is strongly expressed in lung during fetal development and in certain lung cancers. The primary goal of the work presented in this thesis was to investigate whether ESE-1 is involved in regeneration of the injured lung epithelium by administering naphthalene to both wild-type (Elf3 +/+) and Elf3-deficient (Elf3 -/-) mice. However, optimal conditions for proper utilization of the naphthalene-induced lung injury model must first be established. Therefore, dose-response studies were initially conducted by administering three different doses of naphthalene to both male and female mice, as described in chapter 2. Although it is shown that the extent of naphthalene-induced Clara cell injury is dose-dependent in both male and female mice, female mice are more sensitive to naphthalene-induced injury than male mice independent of the dose. Furthermore, it is also demonstrated that these gender-dependent differences in naphthalene injury can subsequently influence downstream lung repair kinetics. In light of these findings, lung regeneration was examined in both sexes of both Elf3 +/+ and Elf3 -/- mice. As reported in chapter 3, the kinetics of bronchiolar epithelial cell proliferation and differentiation is delayed considerably in Elf3 -/- mice following naphthalene injury. Moreover, expression of transforming growth factor-beta type II receptor, which is a well-known transcriptional target gene of ESE-1 and is involved in the induction of epithelial cell differentiation, is significantly lower in the bronchiolar airway epithelium of Elf3 -/- mice as compared to Elf3 +/+ mice under steady-state conditions and during repair of naphthalene-induced damage. Collectively, these findings occur to a similar extent in both sexes of both Elf3 +/+ and Elf3 -/- mice, and suggest that ESE-1 plays an important role in regulating the kinetics of airway epithelial regeneration after acute lung injury.

Human epithelium-specific ETS transcription factor-1 (ESE-1), which is also known as E74-like factor-3 (Elf3) in mice, is strongly expressed in lung during fetal development and in certain lung cancers. The primary goal of the work presented in this thesis was to investigate whether ESE-1 is involved in regeneration of the injured lung epithelium by administering naphthalene to both wild-type (Elf3 +/+) and Elf3-deficient (Elf3 -/-) mice. However, optimal conditions for proper utilization of the naphthalene-induced lung injury model must first be established. Therefore, dose-response studies were initially conducted by administering three different doses of naphthalene to both male and female mice, as described in chapter 2. Although it is shown that the extent of naphthalene-induced Clara cell injury is dose-dependent in both male and female mice, female mice are more sensitive to naphthalene-induced injury than male mice independent of the dose. Furthermore, it is also demonstrated that these gender-dependent differences in naphthalene injury can subsequently influence downstream lung repair kinetics. In light of these findings, lung regeneration was examined in both sexes of both Elf3 +/+ and Elf3 -/- mice. As reported in chapter 3, the kinetics of bronchiolar epithelial cell proliferation and differentiation is delayed considerably in Elf3 -/- mice following naphthalene injury. Moreover, expression of transforming growth factor-beta type II receptor, which is a well-known transcriptional target gene of ESE-1 and is involved in the induction of epithelial cell differentiation, is significantly lower in the bronchiolar airway epithelium of Elf3 -/- mice as compared to Elf3 +/+ mice under steady-state conditions and during repair of naphthalene-induced damage. Collectively, these findings occur to a similar extent in both sexes of both Elf3 +/+ and Elf3 -/- mice, and suggest that ESE-1 plays an important role in regulating the kinetics of airway epithelial regeneration after acute lung injury.

With Internet Explorer 10, Microsoft changed the way of storing web related information. Instead of the old index.dat files, Internet Explorer 10 uses an ESE database called WebCacheV01.dat to maintain its web cache, history and cookies. This database contains a wealth of information that can be of great interest to a forensic investigator. This thesis explores the structure of the new database, what information it contains, how it behaves in different situations, and also shows that it is possible to recover deleted database records – even when the InPrivate browsing mode has been used.

During the maturation of pre-mRNA introns are removed and exons are spliced together, to form a primary transcript, a reaction that is catalyzed by the spliceosome. Alternative splicing is a complex reaction that mainly utilizes one of four mechanisms; exon skipping, 5’ splice site choice, 3’ splice site choice and intron retention. To achieve accurate splicing four sequence elements are essential, two of which are located in the splice sites themselves; 5’ splice sites and 3’ splice sites, but also the polypyrimidine tract and the branch point sequence. Alternative splicing can be regulated by histone or chromatin modulations, siRNA, transcription efficiency and various proteins, many of which belong to either the SR protein family or the hnRNP family of proteins. SR proteins usually promote exon inclusion, while hnRNP proteins usually promote exon skipping. There are also regulatory elements that are called exonic splicing enhancers or silencers depending on if they promote or inhibit the inclusion of the exon they reside in. These elements also exist in introns and are then called intronic splicing enhancers or silencers. The enhancer elements are most commonly targeted by SR proteins and the silencer elements are usually targeted by hnRNP proteins. This paper will mainly focus on the regulation of alternative splicing and the role of alternative splicing under abnormal conditions, such as when mutations cause disease.