Induction of type I interferons by the transcription factors IRF3 and IRF7 is essential in the initiation of antiviral innate immunity. Activation of IRF3/7 requires C-terminal phosphorylation by the upstream kinases TBK1/IKKi, where IRF3/7 phosphorylation promotes dimerization, and subsequent nuclear translocation to the IFN-beta promoter. Recent studies have described the ubiquitin-editing enzyme A20 as a negative regulator of IRF3 signaling by associating with TBK1/IKKi, however the regulatory mechanism of A20 inhibition remains unclear. Here we describe the adaptor protein, TAX1BP1, as a key regulator of A20 function in terminating signaling to IRF3. Murine embryonic fibroblasts (MEFs) deficient in TAX1BP1 displayed increased amounts of IFN-beta production upon viral challenge compared to WT MEFs. TAX1BP1 inhibited virus-mediated activation of IRF3 at the level of TBK1/IKKi. TAX1BP1 and A20 blocked antiviral signaling by disrupting K63-linked polyubiquitination of TBK1/IKKi independently of the A20 deubiquitination (DUB) domain. Furthermore, TAX1BP1 was required for A20 effector function as A20 was defective for the targeting and inactivation of TBK1 and IKKi in Tax1bp1–/– MEFs. Additionally, we found the E3 ubiquitin ligase TRAF3 to play a critical role in promoting TBK1/IKKi ubiquitination. Collectively, our results demonstrate TBK1/IKKi to be novel substrates for A20 and further identifies a novel mechanism whereby A20 and TAX1BP1 restrict antiviral signaling by disrupting a TRAF3/TBK1/IKKi signaling complex. Several viruses utilize a number of strategies to evade the host innate immune response by inhibiting the production of type I interferons. The Human T-cell leukemia virus type 1 (HTLV-1) has been shown to block interferon signaling, however the mechanism of inhibition is poorly understood. We show here that the HTLV-1 encoded protein, Tax plays a critical role in blunting the activation of type I interferons. Tax expression rendered MEFs hyper-permissive in supporting virus replication. Correspondingly, Tax blocked the production of IFN-beta. Interestingly, Tax did not require NEMO interaction to inhibit antiviral signaling to IRF3/7. Instead, Tax targeted RIP1 and further blocked IRF7 K63-linked polyubiquitination. Altogether, we show that Tax inhibits IFN activation by disrupting the ubiquitin dependent activation of IRF7 mediated by RIP1.

This creative project presents the first three modules of a proposed fifteen-module textbook on copy editing. The three modules contain college-level instruction and practice in the elements of copy editing, the tools of the profession, basic line editing, and guides for attribution of news sources and the editing of biased news copy. At the end of each module, there are lists of important terms contained in the text and a series of three editing exercises concentrating on subject matter in the text.The creative project also includes an up-to-date examination of modern newspaper technology and how it affects the copy editor. This examination explores video display terminals, optical character recognition and pagination, including examples of how the editing process is conducted in the computer age.

No description available.

Ivani Kotait's Thesis (mestrado)--Fundação Escola de Sociologia e Política de São Paulo, 1980. / Errata slip inserted. Includes bibliographical references (p. [109]-112) and index.

Thesis (Ph.D.)--Duquesne University, 2009. / Title from document title page. Abstract included in electronic submission form. Includes bibliographical references (p. 333-361) and index.

Previous studies have analyzed how the adoption of genetically engineered or modified technologies have affected agricultural crops such as corn, soybeans, cotton, and barley without focusing on wheat. Also, given the negative impact of drought on wheat production, no studies have focused on the implications of drought tolerant (HB4) and CRISPR/gene-editing on wheat trade. To address these issues, this study employed the partial equilibrium analysis and analyzed the implications of drought tolerant (HB4) and CRISPR/gene-editing technology adoption on wheat trade under various scenarios. The study found that when Argentina, Australia, United States, Canada, and Russia adopt gene-editing wheat, all consuming countries experience a welfare gain except Japan, Korea, Belgium, Netherland, and Italy. More so, Argentina, Mexico, Nigeria, Brazil, Egypt, and Venezuela continue to consume CRISPR wheat in all scenarios. Also, all producing countries experience a gain in producer welfare.

RNA editing had been considered as a rare exception to the central dogma of molecular biology in which the mRNA truthfully carries genetic code from nucleus to the ribosome for translation. However, researches in the last five years have revealed numerous, widespread RNA A-to-I editing sites in human genome. Although the effects of these editing events require further study, this finding strongly suggests RNA editing occurs frequently, and affects large number of genes. By selectively modifying a few sequences of a gene, RNA editing allows a cell to produce a population of proteins with different properties from a single gene. The major question of this thesis study is whether such editing event is actually dynamically regulated when the cellular physiological processes have to be adjusted in response to changing environment. A previous study screening for Drosophila mutants defected in hypoxia and heat tolerance discovered a hypnos-2 mutant strain which was later found to be defective in dADAR, the drosophila gene encoding the A-to-I editing enzyme, supporting the hypothesis that cells/organisms response to stressful environment by dADAR-mediated RNA editing. Two directions are used to approach how Drosophila uses A-to-I editing to adapt ¡§heat¡¨ environment stress. First, whether the expression pattern of dADAR changes after heat shock was investegated. The result showed the dADAR gene exon 7 self-editing frequency was decreased by heat shock, thus possibly enhances dADAR activity after heat shock processing. Moreover it is worth noting that the isoform without -1 exon transcript were obviously up-regulated, and transcript with -1 sequence is relatively down-regulated. On the other hand, no significant changes in the dADAR mRNA expression levels and in the degrees of two dADAR promoters activity were observed. Second, the changes of editing frequency of 30 known A-to-I editing sites were investigated. Generally the editing frequency of majority editing sites changed after heat shock. Therefore, the dADAR activity, the dADAR gene transcript expression alternations, and A-to-I editing frequency of dADAR target genes did change after heat shock, supporting the notion that change of RNA editing pattern is a mechanism for organism to adapt to drastic environmental change. However, how the edited protein isoforms contribute to heat resistance requires further investigation.

Multi-dimensional digital signals have become an intertwined part of day to day life, from digital images and videos used to capture and share life experiences, to more powerful scene representations such as light field images, which open the gate to previously challenging tasks, such as post capture refocusing or eliminating visible occlusions from a scene. This dissertation delves into the world of multi-dimensional signal processing and introduces a tool of particular use for gradient based solutions of well-known signal processing problems. Specifically, a technique to reconstruct a signal from a given gradient data set is developed in the case of two dimensional (2-D), three dimensional (3-D) and four dimensional (4-D) digital signals. The reconstruction technique is multiresolution in nature, and begins by using the given gradient to generate a multi-dimensional Haar wavelet decomposition of the signals of interest, and then reconstructs the signal by Haar wavelet synthesis, performed on successive resolution levels. The challenges in developing this technique are non-trivial and are brought about by the applications at hand. For example, in video content replacement, the gradient data from which a video sequence needs to be reconstructed is a combination of gradient values that belong to different video sequences. In most cases, such operations disrupt the conservative nature of the gradient data set. The effects of the non-conservative nature of the newly generated gradient data set are attenuated by using an iterative Poisson solver at each resolution level during the reconstruction. A second and more important challenge is brought about by the increase in signal dimensionality. In a previous approach, an intermediate extended signal with symmetric region of support is obtained, and the signal of interest is extracted from it. This approach is reasonable in 2-D, but becomes less appealing as the signal dimensionality increases. To avoid generating data that is then discarded, a new approach is proposed, in which signal extension is no longer performed. Instead, different procedures are suggested to generate a non-symmetric Haar wavelet decomposition of the signals of interest. In the case of 2-D and 3-D signals, ways to obtain this decomposition exactly from the given gradient data and the average value of the signal are proposed. In addition, ways to approximate a subset of decomposition coefficients are introduced and the visual consequences of such approximations are studied in the special case of 2-D digital images. Several ways to approximate the same subset of decomposition coefficients are developed in the special case of 4-D light field images. Experiments run on various 2-D, 3-D and 4-D test signals are included to provide an insight on the performance of the reconstruction technique. The value of the multi-dimensional reconstruction technique is then demonstrated by including it in a number of signal processing applications. First, an efficient algorithm is developed with the purpose of combining information from the gradient of a set of 2-D images with different regions in focus or different exposure times, with the purpose of generating an all-in-focus image or revealing details that were lost due to improper exposure setting. Moving on to 3-D signal processing applications, two video editing problems are studied and gradient based solutions are presented. In the first one, the objective is to seamlessly place content from one video sequence in another, while in the second one, to combine elements from two video sequences and generate a transparency effect. Lastly, a gradient based technique for editing 4-D scene representations (light fields) is presented, as well as a technique to combine information from two light fields with the purpose of generating a light field with more details of the imaged scene. All these applications show that the developed technique is a reliable tool for gradient domain based solutions of signal processing problems. / Graduate

Este trabalho estuda o campo da edição de texto na produção editorial de livros no Brasil. O objetivo é apontar algumas distinções necessárias às pesquisas acadêmicas sobre edição de textos, e importantes para a formação de pesquisadores e profissionais. A proposta centra-se nas principais etapas (edição de texto, preparação de originais e revisão de provas) que compõem o processo por que passa um texto a ser transformado em livro. Parte-se de análise dos manuais de editoração e de pesquisas acadêmicas que tangenciam o tema. E articula-se um diálogo entre alguns trabalhos acadêmicos das áreas de psicolinguística e psicologia cognitiva sobre leitura em suas relações possíveis com os estudos em editoração e a experiência empírica dos editores de texto. Contribui-se, assim, para o aprimoramento dos conhecimentos sobre a edição de texto, entre eles: as etapas, os objetivos principais e secundários, as estratégias, as tarefas e um conjunto de elementos necessários para que um profissional atue como editor de texto consciente de sua intervenção. / It is the purpose of this thesis to study the field of text-editing in the Brazilian book publishing industry. Its main aim is to point out a few distinctions deemed necessary not only to the academic research but also to the formation of researchers and professionals in this particular field of study and work. In order to achieve this goal, the main phases the text goes through on its way to becoming a book -- text-editing, copy-editing and proofreading -- are here taken into account and characterized. Examining the available manuals and academic papers on the book editing field is the starting point of this research. It then goes on to promoting a dialogue between the book editing field and other areas of expertise, such as psycholinguistics and cognitive psychology, that also deal with the process of reading. The possible relationships this dialogue reveals and the actual praxis of text editing are then analyzed. Contributing to a better knowledge of what text-editing actually involves is therefore a primary aim of this thesis. It intends to shed some light on the specific phases, primary and secondary goals and strategies text-editing comprises, as well as on a series of elements needed not only for the praxis of text-editing itself but also for a more conscientious practice of this activity.

Este trabalho estuda o campo da edição de texto na produção editorial de livros no Brasil. O objetivo é apontar algumas distinções necessárias às pesquisas acadêmicas sobre edição de textos, e importantes para a formação de pesquisadores e profissionais. A proposta centra-se nas principais etapas (edição de texto, preparação de originais e revisão de provas) que compõem o processo por que passa um texto a ser transformado em livro. Parte-se de análise dos manuais de editoração e de pesquisas acadêmicas que tangenciam o tema. E articula-se um diálogo entre alguns trabalhos acadêmicos das áreas de psicolinguística e psicologia cognitiva sobre leitura em suas relações possíveis com os estudos em editoração e a experiência empírica dos editores de texto. Contribui-se, assim, para o aprimoramento dos conhecimentos sobre a edição de texto, entre eles: as etapas, os objetivos principais e secundários, as estratégias, as tarefas e um conjunto de elementos necessários para que um profissional atue como editor de texto consciente de sua intervenção. / It is the purpose of this thesis to study the field of text-editing in the Brazilian book publishing industry. Its main aim is to point out a few distinctions deemed necessary not only to the academic research but also to the formation of researchers and professionals in this particular field of study and work. In order to achieve this goal, the main phases the text goes through on its way to becoming a book -- text-editing, copy-editing and proofreading -- are here taken into account and characterized. Examining the available manuals and academic papers on the book editing field is the starting point of this research. It then goes on to promoting a dialogue between the book editing field and other areas of expertise, such as psycholinguistics and cognitive psychology, that also deal with the process of reading. The possible relationships this dialogue reveals and the actual praxis of text editing are then analyzed. Contributing to a better knowledge of what text-editing actually involves is therefore a primary aim of this thesis. It intends to shed some light on the specific phases, primary and secondary goals and strategies text-editing comprises, as well as on a series of elements needed not only for the praxis of text-editing itself but also for a more conscientious practice of this activity.