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Effect of different concentrations of n-3 and n-9 fatty acids on fatty acid ethanolamide levels in ratsOlatinsu, Oyindamola Anthonia 16 February 2017 (has links)
Dietary fatty acids are precursors of the lipid mediator group of compounds termed fatty acid ethanolamides (FAE). Prolonged intake of specific types of dietary fats has been shown to increase FAE levels. However, the short term effects of qualitative dietary fat intake on FAE levels remain understudied. Hence, the objective of this study was to identify the effect of diets containing varying concentrations of n-9 from canola oil (CO) and n-3 fatty acids from DHA rich oil (DRO) on plasma and organ FAE levels after different time points in male Sprague Dawley rats. Sixty-four rats were randomly assigned into four groups and were fed diets containing 40% as energy of either safflower, 95% CO: 5% DRO, 50% CO: 50% DRO and 5% CO: 95% DRO. These diets were consumed within a 2hr window in all groups. Circulating fatty acid and FAE levels were measured at 3, 6, 12 and 24hr within each group. At 3hr, significant differences (p<0.05) in plasma oleoylethanolamide (OEA) levels were seen in the 95% CO group: 5% DRO group and 5% CO group: 95% DRO group as well as between 50% canola oil group: 50% DRO and 5% CO group: 95% DRO. In all dietary groups, palmitoylethanolamide (PEA) levels were not significantly different at 3, 6 and 24hr compared to 0hr, but did at 12hr where the 50% CO:50% DRO group showed significantly lower levels than seen in the 95% CO group, but PEA levels were not different from the 5% canola oil group. Although plasma FAE levels were generally multiple times lower than observed in small intestine, liver or brain, arachidonoylethanolamide (AEA) levels were significantly lower in the 95% DRO group than in the remaining two groups. Plasma docosahexanoylethanolamide (DHEA) showed no difference across all time points except at 24hr where levels were higher (p<0.05) in the 95% DRO group than in the remaining two groups. In liver at 3hr, OEA levels were higher (p<0.05) in the 95% CO group than the groups with lesser concentrations of oleic acid, while liver OEA levels showed no difference at any other time points across dietary groups. LEA levels were higher in 95% CO: 5% DRO group compared to the 5% CO group: 95% DRO group after 3hr of feeding. Liver DHEA levels were observed to be highest in the 5% CO group: 95% DRO group at 3 and 12, but not at 6 or 24hr. The dietary fatty acid composition affects plasma and organ fatty acid profiles in a time dependent manner and also produces time shifts in plasma and organ FAE levels. These dietary induced changes according to time points in the levels of FAEs may translate into discernible changes in energy expenditure and lipid levels which may in turn influence the risk of obesity. / February 2017
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Role of Fatty Acid Ethanolamides in PlantsKilaru, Aruna 01 January 2016 (has links)
No description available.
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Mechanisms of action of dietary fatty acids in a syrian hamster model: the role of fatty acid ethanolamides on feeding intake, body composition and energy expenditureLin, Lyyn (Lin) 11 April 2011 (has links)
Replacement of saturated fatty acids (SFA) with monounsaturated fatty acids (MUFA) or polyunsaturated fatty acids (PUFA) impacts risk of atherosclerosis and cardiovascular disease (CVD). However, although dietary fatty acids (DFA) have been established as an important factor related to CVD, their exact mechanisms of action have not been clearly established. One of the possible mechanisms is that DFA convert to fatty acid ethanolamides (FAEs), such as oleoylethanolamide (OEA), palmitoylethanolamide (PEA) and arachidonoylethanolamide (AEA), which are thought to associate with lipid signalling, fat oxidation and appetite control. Hence, the objectives of this thesis were to identify the impact of diets containing corn oil, canola oil, DHA + canola oil and fish oil on plasma and organ levels of FAEs as well as energy metabolism and lipid profiles in Syrian Golden hamsters. Forty-eight hamsters were provided diets containing 6% treatment oil for 30 d before sacrifice. Across all diets, in proximal small intestine and liver, animals fed canola oil showed higher (p<0.05) levels of OEA than corn oil and fish oil fed groups, but no difference compared to those fed DHA +canola oil. In plasma, fish oil fed animals showed higher (p<0.05) OEA and PEA levels and lower (p<0.05) AEA levels compared to all other groups. Feed intakes (g/d), oxygen consumption (ml/g) and body composition of total fat (%) and mass (g) did not differ across groups. However, energy expenditure associated with fat oxidation (%) was higher (p<0.01) in canola oil and DHA + canola oil fed hamsters compared to those consuming corn oil and fish oil. Also, body composition of fish oil fed animals showed a lower (p<0.01) total lean mass (g) compared to other three groups and a lower (p<0.01) total mass (g) compared to DHA + canola oil diets, but no difference compared to animals fed the canola oil diet. None of the treatments had any effect on triglyceride (TG) or C-reactive protein (CRP) levels. The fish oil group showed a higher (p<0.01) plasma total cholesterol (TC) levels than all other three groups. No differences existed between DHA + canola oil and fish oil groups in HDL or Non-HDL levels, but these levels were different (p<0.01) compared to corn oil group and canola oil groups. To conclude, different DFA affect whole body energetics and plasma lipid profiles. Also DFA produced marked shifts in plasma and organ levels of OEA, PEA and AEA. These dietary induced shifts in FAEs may translate into discernable changes in energy expenditure and lipid levels which in turn influence CVD risk.
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Mechanisms of action of dietary fatty acids in a syrian hamster model: the role of fatty acid ethanolamides on feeding intake, body composition and energy expenditureLin, Lyyn (Lin) 11 April 2011 (has links)
Replacement of saturated fatty acids (SFA) with monounsaturated fatty acids (MUFA) or polyunsaturated fatty acids (PUFA) impacts risk of atherosclerosis and cardiovascular disease (CVD). However, although dietary fatty acids (DFA) have been established as an important factor related to CVD, their exact mechanisms of action have not been clearly established. One of the possible mechanisms is that DFA convert to fatty acid ethanolamides (FAEs), such as oleoylethanolamide (OEA), palmitoylethanolamide (PEA) and arachidonoylethanolamide (AEA), which are thought to associate with lipid signalling, fat oxidation and appetite control. Hence, the objectives of this thesis were to identify the impact of diets containing corn oil, canola oil, DHA + canola oil and fish oil on plasma and organ levels of FAEs as well as energy metabolism and lipid profiles in Syrian Golden hamsters. Forty-eight hamsters were provided diets containing 6% treatment oil for 30 d before sacrifice. Across all diets, in proximal small intestine and liver, animals fed canola oil showed higher (p<0.05) levels of OEA than corn oil and fish oil fed groups, but no difference compared to those fed DHA +canola oil. In plasma, fish oil fed animals showed higher (p<0.05) OEA and PEA levels and lower (p<0.05) AEA levels compared to all other groups. Feed intakes (g/d), oxygen consumption (ml/g) and body composition of total fat (%) and mass (g) did not differ across groups. However, energy expenditure associated with fat oxidation (%) was higher (p<0.01) in canola oil and DHA + canola oil fed hamsters compared to those consuming corn oil and fish oil. Also, body composition of fish oil fed animals showed a lower (p<0.01) total lean mass (g) compared to other three groups and a lower (p<0.01) total mass (g) compared to DHA + canola oil diets, but no difference compared to animals fed the canola oil diet. None of the treatments had any effect on triglyceride (TG) or C-reactive protein (CRP) levels. The fish oil group showed a higher (p<0.01) plasma total cholesterol (TC) levels than all other three groups. No differences existed between DHA + canola oil and fish oil groups in HDL or Non-HDL levels, but these levels were different (p<0.01) compared to corn oil group and canola oil groups. To conclude, different DFA affect whole body energetics and plasma lipid profiles. Also DFA produced marked shifts in plasma and organ levels of OEA, PEA and AEA. These dietary induced shifts in FAEs may translate into discernable changes in energy expenditure and lipid levels which in turn influence CVD risk.
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The Effect of Consuming Canola and Flax Oils in Modulation of Vascular Function and Biomarkers of Cardiovascular Disease RisksThe Effect of Consuming Canola and Flax Oils in Modulation of Vascular Function and Biomarkers of Cardiovascular Disease RisksPu, Shuaihua 14 May 2014 (has links)
It is well established that replacing dietary saturated fatty acids with unsaturated fatty acids reduces cardiovascular disease (CVD) risk. Although epidemiological and clinical evidence indicate health benefits of consuming various fatty acid classes including n-9, n-6, and short- and longer-chain n-3 fatty acids, current dietary recommendations fall short of providing the optimal amounts of these fatty acids in daily diets. In addition, significant knowledge gaps remain in our understanding of the effects of, and mechanisms underpinning the action of, the various fatty acid classes on risk factors for CVD. The objective of this research was to contribute to the evaluation of health benefits of using different dietary oils, and determine how these benefits may play a role in improving public health and decreasing CVD risk. Additionally, this research examined effects of diet-gene interactions, endogenous fatty acid ethanolamides (FAEs) on body fat mass distribution as well as changes in the composition of gut microbiota following consumption of dietary oil treatments. The Canola Oil Multicenter Intervention Trial (COMIT) was conducted as a 5-phase randomized, controlled, double-blind, cross-over clinical trial. Each 4-wk treatment period was separated by 4-wk washout intervals. A total of 130 volunteers with abdominal obesity consumed each of 5 identical weight-maintaining, fixed-composition diets with one of the following treatment oils (60 g/3000 kcal) in the form of beverages: 1) conventional canola oil (Canola; n-9 rich), 2) high–oleic acid canola oil with docosahexaenoic acid (CanolaDHA; n-9 and n-3 rich), 3) a blend of corn and safflower oil (25:75) (CornSaff; n-6 rich), 4) a blend of flax and safflower oils (60:40) (FlaxSaff; n-6 and short-chain n-3 rich), and 5) high–oleic acid canola oil (CanolaOleic; highest in n-9). At endpoints, plasma fatty acid levels reflected the differences in fatty acid composition of five dietary treatments. All diets lowered total cholesterol (TC) compared with baseline. TC was lowest after the FlaxSaff phase and highest after CanolaDHA. The CanolaDHA treatment improved HDL-C, triglycerides, and blood pressure thereby reducing Framingham risk scores compared with other oils varying in unsaturated fatty acid composition. Homozygotes minor allele carriers of rs174583 (TT) on FADS2 gene showed lower (P<0.01) plasma EPA and DPA levels across all diets, but no differences were observed in DHA concentrations after the CanolaDHA feeding. In addition, plasma FAE levels were positively associated with plasma fatty acid profiles. Minor allele A carriers of rs324420 of FAAH gene showed a higher (P<0.05) plasma FAE levels compared with major allele C carriers across all diets, and showed higher (P=0.0002) docosahexaenoylethanolamide levels in response to the CanolaDHA diet. Impacts of consuming 60 g of five dietary oil treatments on gut microbiota composition were relatively minor at the phylum level and mainly at the genus level, while BMI contributed to a significant shift at the phylum level. In conclusion, consumption of a novel DHA-enriched canola oil improved blood lipid profile and largely reduced CVD risk. Diet-gene interactions might help identify sub-populations who appear to benefit from increased consumption of DHA and oleic acid. The metabolic and physiological responses to dietary fatty acids may be influenced via circulating FAEs, while the altered microbiota profile by shifts in MUFA and/or PUFA may be associated with specific physiological effect. Personalized diets varying in unsaturated fatty acids composition based on specific lifestyles, environmental factors, psychosocial factors, and genetic make-ups will become the future “healthy eating” recommendations to prevent CVD risk. / May 2016
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Distribution of Bioactive Lipid Mediators in Human SkinKendall, A.C., Pilkington, S.M., Massey, Karen A., Sassano, G., Rhodes, L.E., Nicolaou, Anna 03 1900 (has links)
No / The skin produces bioactive lipids that participate in physiological and pathological states, including homeostasis, induction, propagation, and resolution of inflammation. However, comprehension of the cutaneous lipid complement, and contribution to differing roles of the epidermal and dermal compartments, remains incomplete. We assessed the profiles of eicosanoids, endocannabinoids, N-acyl ethanolamides, and sphingolipids, in human dermis, epidermis, and suction blister fluid. We identified 18 prostanoids, 12 hydroxy-fatty acids, 9 endocannabinoids and N-acyl ethanolamides, and 21 non-hydroxylated ceramides and sphingoid bases, several demonstrating significantly different expression in the tissues assayed. The array of dermal and epidermal fatty acids was reflected in the lipid mediators produced, whereas similarities between lipid profiles in blister fluid and epidermis indicated a primarily epidermal origin of suction blister fluid. Supplementation with omega-3 fatty acids ex vivo showed that their action is mediated through perturbation of existing species and formation of other anti-inflammatory lipids. These findings demonstrate the diversity of lipid mediators involved in maintaining tissue homeostasis in resting skin and hint at their contribution to signaling, cross-support, and functions of different skin compartments. Profiling lipid mediators in biopsies and suction blister fluid can support studies investigating cutaneous inflammatory responses, dietary manipulation, and skin diseases lacking biomarkers and therapeutic targets.
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Síntese, caracterização e avaliação biológica de compostos anfifílicos obtidos de fontes químicas renováveisFerreira, Bianca da Silva 14 March 2014 (has links)
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Previous issue date: 2014-03-14 / CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior / Este trabalho foi dividido em três capítulos, nos quais foram discutidas a síntese de
caracterização de séries de substâncias anfifílicas obtidas de fontes químicas
renováveis (óleos de pequi, buriti e babaçu, ácidos graxos e carboidratos).
O primeiro capítulo descreve a síntese e caracterização de etanolamidas graxas
obtidas por aminólise de óleos vegetais ou de ésteres metílicos graxos puros com
monoetanolamina e dietanolamina. As propriedades biológicas dos óleos, das
misturas de amidas e das amidas puras foram comparadas. O óleo de Buriti foi o
que apresentou maior atividade antioxidante (7,70 mg/mL) devido a maior
concentração de carotenoides (692,98 μg/g). As amidas derivadas do mesmo óleo
também apresentaram maior atividade antioxidante do que as demais etanolamidas
sintetizadas. A atividade antibacteriana das misturas de amidas e das amidas puras,
avaliada frente a bactérias Gram-positivas e Gram-negativas não foi significativa.
No segundo capítulo é descrita a síntese e caracterização de diaminas N-aciladas
lipofílicas e de aldonamidas derivadas da D-glicono-1,5-lactona da D-ribono-1,4-
lactona ou do éster galactárico. Para obtenção das aldonamidas as lactonas e o
éster galactárico foram tratados com as diaminas N-aciladas, sob refluxo de metanol,
em tempos que variaram de 24-48 horas.
Os compostos sintetizados foram avaliados frente suas propriedades biológicas in
vitro. Foram realizados testes antibacterianos contra bactérias Gram-positivas,
Gram-negativas e bactérias S. aureus meticilino resistentes; e testes antifúngicos
frente quatro espécies diferentes de Candida. A atividade antiinflamatória foi
estudada através da capacidade dos compostos de inibirem a produção de óxido
nítrico por macrófagos ativados; a viabilidade celular foi investigada através do
método do MTT.
As aldonamidas em geral não apresentaram atividade, entretanto as diaminas Naciladas
mostraram atividade acentuada nos testes antibacterianos e antifúngicos.
Os resultados foram comparáveis ou até mesmo superiores aos padrões utilizados
como controles positivos (cloranfenicol e itraconazol). Para alguns compostos, foi
possível estabelecer uma correlação entre lipofilicidade e a atividade antifúngica.
Entretanto, nos testes de viabilidade celular, esses compostos apresentaram-se
tóxicos nas concentrações do CIM.
No terceiro capítulo é descrita a síntese e caracterização de N-acilidrazonas
derivadas da D--glicono-1,5-lactona, D--ribono-1,4-lactona e éster galactárico. Para
obtenção das N-acilidrazonas, as hidrazidas derivadas dos carboidratos foram
tratadas com aldeídos comerciais aromáticos e alifáticos, utilizando como solvente o
metanol a temperatura ambiente. Os tempos de reação variaram de 24-72 horas. Os
compostos foram obtidos na forma de misturas de diastereoisômeros, onde o
isômero E foi o majoritário para todos os derivados. Os compostos sintetizados
também foram submetidos à avaliação de suas propriedades biológicas in vitro.
Foram realizados testes antibacterianos contra bactérias Gram-positivas, Gramnegativas
e testes antifúngicos frente quatro espécies diferentes de Candida,
entretanto os compostos não se mostraram ativos. A atividade anti-inflamatória, bem
como a citotoxicidade desses compostos estão sendo avaliadas no ICB-UFJF.
Ainda nesse último capítulo, foi realizada uma tentativa de ciclização das Nacilidrazonas
para fornecer os seus respectivo, 1,3,4-oxadiazóis. Entretanto os
compostos desejados não puderam ser obtidos pelas metodologias utilizadas. / This work was divided into three chapters, in which was discussed the synthesis and
characterization of amphiphilic compounds derived from renewable chemical sources
(oil Pequi, Buriti and Babassu oil, fatty acids and carbohydrates).
The first section describes the synthesis and characterization of fatty ethanolamides
obtained by aminolysis of vegetable oils or pure methylic fatty esters with
monoethanolamine and diethanolamine. The biological properties of the oils,
mixtures of amides and pure amides were compared . Buriti oil showed the highest
antioxidant activity (7.70 mg/mL) due to the higher concentration of carotenoids
(692.98 mg/g). Amides derived from the same oil also showed higher antioxidant
activity than other synthesized ethanolamides. The antibacterial activity of the
mixtures of amides and amides pure evaluated against Gram-positive and Gramnegative
bacteria was not significant.
The second section describes the synthesis and characterization of lipophilic Nacylated
diamines and aldonamides derived from D-glicono-1,5- lactone D-ribono-1,4-
lactone or ester galactaric. To obtain the aldonamides, lactones and galactaric ester
were treated with N-acylated diamines refluxing methanol in time ranging from 24-48
hours.
The synthesized compounds had their in vitro biological properties evaluated.
Antibacterial test against Gram-positive bacteria, Gram-negative and Methicillin
resistant Staphylococcus aureus were performed, and also antifungal test against
four species of Candida. The anti-inflammatory activity was investigated by the ability
of the compounds to inhibit nitric oxide production by activated macrophages, cell
viability was assessed by the MTT method.
The aldonamides generally showed no activity, but the N-acylated diamines showed
significants activities in antibacterial and antifungal tests. The results were
comparable or even superior to those used as positive controls (chloramphenicol and
itraconazole). For some compounds, it was possible to establish a correlation
between lipophilicity and antifungal activity. However, in the cell viability tests , these
compounds proved to be toxic at MIC.
The third section describes the synthesis and characterization of N-acylhydrazones
derived from D-glicono-1,5-lactone , D-ribono-1,4-lactone and galactaric ester. To
obtain the N-acylhydrazones, the hydrazide derivatives of carbohydrates have been
treated with aromatic aldehydes, using methanol as solvent at room temperature.
The reaction times ranged from 24-72 hours. The compounds were obtained as
mixtures of diastereomers, where E was the major isomer for all derivatives. The
synthesized compounds were also examined for their in vitro biological properties.
Antibacterial test against Gram-positive bacteria, Gram- negative, M. tuberculosis
and antifungal test against four species of Candida were made, however the
compounds were not active. The anti-inflammatory activity and cytotoxicity of these
compounds are being evaluated in ICB-UFJF.
Also in this last chapter, an attempt to cyclization of the N-acylhydrazones was
performed to provide their respective, 1,3,4-oxadiazoles. However the desired
compounds could not be obtained by the methodologies used.
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