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Genomic, expression and functional analysis of genes from larval gut of the European corn borer, Ostrinia nubilalis (Hübner)Khajuria, Chitvan January 1900 (has links)
Doctor of Philosophy / Department of Entomology / Larry L. Buschman / Kun Yan Zhu / Genomic information for lepidopteran insects, particularly agricultural pest species, is very limited but urgently needed due to their economic importance and biodiversity. The huge economic losses ($ 1-2 billons / year) caused by the European corn borer (Ostrinia nubilalis, Hübner, ECB) makes this insect species one of the major pests of corn in the United States and western world. Management of ECB by conventional methods is limited but has had a great success by transgenic Bt (Bacillus thuringiensis) corn, which targets insect gut. However, the widespread use of Bt corn may lead to the development of Bt resistance in ECB. Knowledge of genes expressed in the insect gut is considered crucial for understanding basic physiology of food digestion, their interactions with Bt toxins and pathogens, and for discovering new targets for pest management.
A large database of 15,000 expressed sequence tags (ESTs) was established from the ECB larval gut. To our knowledge, this database represents the largest gut-specific EST database from a lepidopteran pest. Analysis of 10 aminopeptidase-like genes between Cry1Ab–resistant and –susceptible ECB larvae revealed that aminopeptidase P-like (OnAPP) gene is a strong candidate for its role in Bt toxicity and resistance. The RNA interference mediated reduction in the transcript level of OnAPP gene in ECB larvae resulted in their reduced susceptibily to Cry1Ab.
Analysis of the chitinase-like gene (OnCht) revealed its essential role in regulating chitin content of peritrophic membrane (PM). Our results suggest that OnCht may influence food digestion, nutrient absorption or movement of digestive enzymes through the PM and can be an important target for insect management. We also identified and characterized six genes involved in the innate immune defense response in ECB and showed that the expression of these genes were induced when challenged with bacteria.
In addition to these results, this research generated significant genomic information for the development of microarray from the larval gut of ECB. The establishment of the feeding-based RNA interference technique could potentially help in delivering dsRNA orally to ECB for high throughput screening of effective genes to be targeted for insect pest management.
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A comparative study of the effects of pathogens on the European corn borer, Ostrinia nubilalis, in laboratory and field studiesManuszak, John Louis 03 June 2011 (has links)
In some areas the effect of pathogenicity of microorganisms upon the European corn borer have been explored. The microorganisms studied were: Nosema, pyraustae, Nosema necatrix, and Bacillus thuringlensis var, thuringiensie. Most studies have been limited to either laboratory or field work. In this thesis, a comparative study of mortality, percent infection, and tune intervals in which infection occurs have been explored, in the field and the laboratory studies.It is concluded: (1) field recovered borers at different day intervals showed interaction between the experimental microorganisms and time intervals (2) that the microorganisms used in the field, especially N. necatrix, may be used as effective microbial control agents against Ostrinia nubilalis.Ball State UniversityMuncie, IN 47306
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Quantitative trait loci for first- and second-generation European corn borer resistance in maize /Jampatong, Chaba, January 1999 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 1999. / Typescript. Vita. Includes bibliographical references (leaves 72-79). Also available on the Internet.
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Quantitative trait loci for first- and second-generation European corn borer resistance in maizeJampatong, Chaba, January 1999 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 1999. / Typescript. Vita. Includes bibliographical references (leaves 72-79). Also available on the Internet.
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Electrical Capacitance Measurements to Assess European Corn Borer Infestation in MaizeThomas, Mavrik D. 14 April 2021 (has links)
The European Corn Borer (ECB), Ostrinia nubilalis, is an agricultural pest which bores small holes in the rind of maize stalks and then proceeds to consume the pith. Because most of the damage to the stalk is internal, it is difficult to quantify the damage to an individual stalk without time-consuming, manual examination. This work explored the hypothesis that internal damage could be detected and quantified using non-destructive, electromagnetic measurements. Laboratory experiments and numerical simulation studies predicted changes in capacitance of stalks due to hollow core ECB damage. A guarded probe device to measure electrical impedance from 500 Hz to 100 kHz was designed and constructed for data collection. A field test with the measurement device was conducted and frequency-swept impedance measurements were taken on field-grown plants with and without ECB damage. Field measurements demonstrated that statistically significant capacitance changes associated with ECB damage could be detected in agreement with numerical simulations of stalk damage. Numerical, laboratory, and field test results all supported the hypothesis that electromagnetic impedance measurements, in particular, capacitance, provide a promising new avenue for ECB damage evaluation. While further research will be needed to further refine this concept, this measurement approach is non-destructive, thus allowing measurements to be performed without sacrificing the infested plants.
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Use of the egg parasitoid, Trichogramma ostriniae (Hymenoptera: Trichogrammatidae) as a biological control agent of the European corn borer, Ostrinia nubilalis (Lepidoptera: Crambidae): An approach to integrated pest management in bell pepperBarlow, Vonny M. 05 May 2006 (has links)
Four to six separate inundative releases of ~30,000 to 50,000 T. ostriniae per 0.02 ha significantly reduced damage by O. nubilalis in bell pepper. Egg parasitization averaged 48.7% in T. ostriniae release plots, which was significantly higher than non-release plots (1.9%). Also, cumulative green pepper fruit damage averaged 8.7% in release plots, which was significantly less than non-release plots (27.3%). Pesticides tested against T. ostriniae were spinosad and methoxyfenozide. Spinosad adversely affected adult T. ostriniae producing 100% mortality at the field rate of 498 mg [AI]/L for both the preimaginal and adult toxicity tests. Use of T. ostriniae can provide effective control of O. nubilalis in pepper compared to conventional and organic spray regimes (Spinosad and methoxyfenozide respectively). Augmentative releases of T. ostriniae integrated with methoxyfenozide with its limited toxicity to both preimaginal and adult stages indicate that it is a superior candidate for control O. nubilalis.
To determine the behavioral differences of T. ostriniae and its response to O. nubilalis in green bell peppers, experiments were carried out to classify likely areas of O. nubilalis oviposition within the green pepper plant canopy. A total of 426 O. nubilalis egg masses were found on pepper plants during our study. Over 92% of egg masses were found on the lower surface of the leaf compared with the upper surface indicating a significant ovipositional preference for the undersides of leaves in 2002 ( x2 = 9.68; df = 1; P < 0.05) followed by similar results in 2004 (x2 = 4.34; df = 1; P < 0.05). No significant differences were found in the observed spatial distribution of egg masses among the three vertical strata in either 2002 or 2004 (x2 = 1.75; df = 2; P < 0.05 and x2 = 5.69; df = 2; P < 0.05 respectively). Field release rates of 17.0 foraging T. ostriniae females can achieve 80.0% parasitism of O. nubilalis egg masses distributed throughout the pepper plant canopy found primarily on the undersides of leaves. These data demonstrate that T. ostriniae has potential as a biocontrol agent for O. nubilalis in solanaceous crops. / Ph. D.
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The Impact of <i>Beauveria Bassiana</i>, <i>Trichogramma</i>, <i>Bt Sprays</i> and Spinosad on the Lepidopteran (Crambidae) Cereal Stalk Borer, the European Corn Borer (<i>Ostrinia Nubilalis</i>)Tembo, Rostern N. 02 May 2009 (has links)
No description available.
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Application of Bacillus Thuringiensis through center-pivot irrigation systems for control of Southwestern and European corn borerNolting, Steven P January 2011 (has links)
Digitized by Kansas Correctional Industries
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The effects of per os doses of Bacillus thuringiensis and Bacillus megaterium upon the hemocytes of the fifth instar European corn borer, Ostrinia nubilalis (H�ubner) (Lepidoptera: Pyralidae)Reames, Spencer Eugene 03 June 2011 (has links)
Hemocytes of the fifth instar European borer, Ostrinia nubilalis Hubner (Lepidoptera: Pyralidae) were examined in stained and unstained preparations. Prohemocytes, plasmatocytes, granular hemocyter, and oenocytoids, and spherule cells were found in this stage, The prohemocytes are characterized by a scant intensely basophilic cytoplasm. The highly pleomorphic plasiratocytes are characterized by a punctate nucleus and production of cytoplasmic extensions in vitro. Granular hemocytes are characterized by a small nucleus, accumulation of lipid droplets, and the production of extremely fine cytoplasmic extensions in living preparations. The oenocytoid is characterized by a small eccentric nucleus in a large amount of homogeneous basophilic cytoplasm. The spherule cell is characterized by large spherules within the cytoplasm which may tend to mask the nucleus.Treatment of larvae with per os doses of Bacillus thuringiensis did not affect the differential hemocyte count. However, there did appear to be an increase in the number of degenerating cells in Bacillus thuringiensis treated groups.Ball State UniversityMuncie, IN 47306
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The Effects of European corn borer on whole-plant yieldand root knot nematode fitness in cornTiwari, Siddharth 07 May 2007 (has links)
Field studies were conducted over two growing seasons to evaluate the effect of different levels of third instar European corn borer, Ostrinia nubilalis Hübner (Lepidoptera: Crambidae), on whole-plant dry matter in corn grown for silage. Mean (± SEM) whole-plant dry matter was significantly greater by 18.8% in uninfested control plants than in plants with an infestation level of 6 larvae/plant in 2004. Whole-plant dry matter in 2005 was significantly greater by 10.5% in control plants than in plants with an infestation level of 5 larvae/plant. Economic injury levels were calculated for each year using regression equations between whole-plant dry matter and European corn borer infestation level. Plant growth stage and infestation level had no effect on percent acid detergent fiber, neutral detergent fiber, and crude protein values for either year.
Greenhouse studies were conducted to examine the relationship between aboveground herbivory by European corn borer and belowground herbivory by root knot nematode, Meloidogyne incognita Chitwood (Tylenchida: Heteroderidae), in corn. Two experiments were conducted to measure belowground herbivory by M. incognita in juvenile penetrations and eggs/root system. In the first experiment, the main effects interaction was not significant for either M. incognita juvenile penetrations or eggs/root system. Overall mean juvenile penetrations/root system across all three growth stages, at infestation levels of 1 and 3 larvae/plant were significantly less than in the non-infested control. In addition, overall mean eggs/root system at an infestation level of 3 larvae/plant were significantly less than in the control. In the second experiment, the main effects interaction was significant for both juvenile penetrations and eggs/root system. At the 8 and 10 leaf growth stages, juvenile penetrations/root system at infestation levels of 1 and 3 larvae/plant were significantly less than in the control. In addition, eggs/root system at an infestation level of 3 larvae/plant were significantly less than in the control, at all growth stages. In the reciprocal study, which examined the effect of different M. incognita inoculation levels on European corn borer stalk tunneling, no significant effect of inoculation level on European corn borer stalk tunneling was found. / Ph. D.
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