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Studies on the regulation of mitotic transition by cyclin B1/Cdk1Soni, Deena V. January 2005 (has links)
No description available.
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Attachment of Streptococcus pyogenes to Host Epithelial CellsSethman, Chad Robert 19 December 2003 (has links)
No description available.
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Quantitative, Multiparameter Analysis of Fluorescently Stained, Negatively Enriched, Peripheral Blood from Cancer PatientsMiller, Brandon Lee January 2013 (has links)
No description available.
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Characterization of Sialic Acid Receptors on MDCK Cells Maintained Under Different Media Conditions by Flow Cytometric Analysis and Implications for Detection of Influenza A Virus.Nelson, Sarah W. 29 August 2016 (has links)
No description available.
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Angiogenic Characteristics of Tumor-Associated Dendritic Cells in Ovarian and Breast Cancer ModelsLewis, Deana L. January 2016 (has links)
No description available.
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Development of Novel Fluorescence-Based Methods for Detection of Bacillus Anthracis SporesSchumacher, William Charles 29 September 2008 (has links)
No description available.
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Cell Damage Mechanisms and Stress Response in Animal Cell CultureBerdugo, Claudia 25 August 2010 (has links)
No description available.
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Modulation of cell-cycle associated antigen expression by the B16 melanoma : multiparameter analysis using monoclonal antibodies and flow cytometry /Trimpe, Kevin L. January 1985 (has links)
No description available.
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THE EFFECT OF ACUTE EXERCISE ON THE PRODUCTION OF REACTIVE OXYGEN SPECIES AND INFLAMMATORY MARKERS IN HEALTHY PRE-PUBERTAL AND ADULT MALESLiu, Maple 10 1900 (has links)
<p>An acute bout of exercise causes short-term changes in the immune system in both children and adults. It has been well-established that exercise induces an inflammatory response. Especially in children, cytokines play an important role in balancing anabolic and catabolic processes of growth. Existing evidence suggests cross-talk between inflammation and oxidative stress. Reactive oxygen species are also found to transiently increase in response to exercise, affecting muscle adaptation post-exercise. Characterizing the exercise-induced inflammatory and oxidative stress responses in children compared to adults will start clarifying the transition from the child phenotype to that of an adult. Ten children aged 8-10 and 12 adults aged 19-21 performed 2×30min bouts of continuous cycling, separated by a 6min rest period, at a target work rate of 60% of their maximum aerobic capacity. Blood samples were collected pre-exercise and immediately post-exercise, and analyzed for<strong> </strong>neutrophil count, systemic oxidative and inflammatory markers (tumor necrosis factor alpha, interleukin 6, protein carbonyls, malondialdehyde, elastase), intracellular neutrophil-derived reactive oxygen species (using 3 fluorescent markers detected by flow cytometry), and <em>in vitro</em> production of neutrophil-derived myeloperoxidase and interleukin 8. Compared to the post-exercise increase in absolute neutrophils in men, boys showed no change. However, intracellular neutrophil reactive oxygen species production increased for boys and not for men. Boys also demonstrated higher overall protein carbonyl levels, whereas men showed higher overall malondialdehyde. Both boys and men showed a positive correlation between tumor necrosis factor alpha and elastase, with a steeper slope seen in boys. Although there were other correlations observed in boys and men, no others existed in both. The differences observed in the exercise-induced inflammatory and oxidative stress response may indicate growth-mediated adaptive responses to exercise during childhood development.</p> / Master of Science (MSc)
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Development of a Microchip-Based Flow Cytometer with Integrated Optics – Device Design, Fabrication, and TestingWatts, Benjamin 04 1900 (has links)
<p>Lab-on-a-chip technologies have created a burgeoning number of new and novel devices designed to automate biological processes on-chip in an efficient and inexpensive format for far reaching point-of-care (POC) medicine and diagnostic treatments and for remote and on-line monitoring functions. This work designed a device that integrated advanced optical functionality on-chip with the microfluidics to relieve the reliance on traditional bulky and expensive free-space optics and a high-quality light source. The multimodal input beam was reshaped into an optimized geometry in the microchannel via a 2D system of lenses - improving the quality and reliability of detection through uniform detection of particles. A uniform beam geometry across the sample stream with a uniform beam width will allow repeatable excitation and burst duration to allow for more reliable and predictable detection. Numerous beam geometries were created and the quality and illumination properties confirmed by testing each with a couple sizes of fluorescent and non-fluorescent microspheres to test the effect of beam geometry and particle size combination on device performance. The measured coefficient of variation (CV) for fluorescent beads was found to have a particular beam geometry that yielded best device performance based on the bead size. Fluorescent beads 2.5µm in diameter had a CV of 8.5% for a 3.6 µm beam waist while 6 µm beads yielded a 14.6% CV with a 10 µm beam waist. When measuring scatter and fluorescence signal from a 10 µm the 2.5- and 6.0 µm beads gave 11.4% and 15.8% and 15.9% and 20.4% fluorescent and scatter CVs for each set of beads, respectively. Separately testing each beam geometry with 1-, 2-, and 5 µm beads did not yield any predictable ideal beam-bead ideal pairing for best performance. Lastly, further integration of optical function was shown through the on-chip collection of signals; CVs of 29% and 30% were measured for side scatter and forward scatter, respectively, for 5 µm beads. The reliability of this all-optically guided scheme was confirmed by comparing it to a simultaneously recorded free-space collection scheme. The coincidence rate was found to be 94% and 96% for the side scatter and forward scatter schemes. Both had very low false positive rates – below 0.5% - with missed detection rates that were satisfactory but in need of improvement. Sources of noise and device improvements were identified and suggested.</p> / Doctor of Philosophy (PhD)
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