An affinity based bio-sensing technique was developed using an anti-transmissible spongiform encephalopathy monoclonal antibody as a bio-recognition molecule. Fluorescein iso-thio-cynate (FITC), labeled with a prion epitope (QYQRES), was used as a decoy for prions. Experiments done in 0.1M phosphate buffer revealed that the dye fluorescence increased with the pH of the buffer and was influenced by solvent polarity. Binding studies conducted at pH 6, 7, and 8 showed that the optimum pH for the antibody-decoy binding was 7. Maximum differences between control and antibody samples were observed at pH 7. The optimum incubation time was found to be less than 4 hours for the control, antibody, and the prion samples at room temperature. Prion detection curves were established at 4 and 10 nM antibody decoy concentrations. The lowest detectable prion concentration in phosphate buffer was 8 nM. Experimental conditions determined in the phosphate buffer were used to implement the technique in gelatin and baby formula. Prion detection curves were generated in 0.01, 0.4, 1.0 and 2.0 mg/ml of gelatin solution. The gelatin interfered with the binding and the displacement reaction of antibody, decoy and prion. Addition of an anionic surfactant, sodium dodecyl sulfate (SDS) at 0.3 mg/ml to gelatin samples facilitated prion detection in gelatin. The lowest detectable concentration of prion in gelatin was 0.5 nM at 0.4mg/ml gelatin. The baby formula samples produced light scattering and the intrinsic peak of baby formula at 526nm interfered with the dye peak at 514nm. Serial dilutions of baby formula were done to reduce the interference. Prion detection curves were then obtained at 1.31 and 5.34 mg/ml baby formula and 0.454 mg/ml of Triton-X-100 was added to the baby formula samples. The lowest detectable concentration of prion was 2 nM for baby formula. This developed bio-sensing technique can be used to detect prion in gelatin and baby formula solutions. Addition of surfactants assisted prion detection in foods, while high concentrations of gelatin and baby formula had an adverse effect on the detection system.

Thesis (Ph. D.)--University of California, San Diego, 2008. / Available via ProQuest Digital Dissertations. Vita. Includes bibliographical references (p. 293-308).

Thesis (M.S.)--Kent State University, 2009. / Title from PDF t.p. (viewed Jan. 12, 2010). Advisor: Natalie Caine-Bish. Keywords: Includes bibliographical references (p.59-64).

Thesis--PlanA (M.S.)--University of Wisconsin--Stout, 2003. / Includes bibliographical references.

Thesis (M. Phil.)--University of Hong Kong, 2002. / Includes bibliographical references (leaves.

Tesis de grado para obtener el titulo de Licenciatura en Biologia, Universidad Mayor de San Simon, Facultad de Ciencias Y Tecnologia, Carrera de Biologia. / Abstract in Spanish and English.

Introduction: The Genetically Modified (GM) food, which is one of the fruit of the modern biotechnology, is closely related to people's lives. GM food, specifically, GM crops, also known as biotech food, are produced from genetically modified organisms (GMO), which use genetic engineering techniques to introduce, recombine and modify DNA. The safety of GM food still do not have final conclusion at present. Although GM food has been introduced into China for over 15 years, many of the surveys show that Chinese consumers‘ knowledge of GM food is relatively low comparing with other countries. In 2002, China‘s Ministry of Agriculture promulgated three regulations to manage the GMOs in China. The attitudes and acceptance of market for GM food have direct impact on the development of genetic engineering technique and government‘s policy making. This review aims to investigate cognition and attitudes of GM food among Chinese consumers; to investigate how the consumers react to the GM food labeling policy; to find out how factors such as knowledge about transgenic information, price of GM food would affect the consumption of GM food. Methods: Relevant studies published between January 2002 and May 2013 were searched and identified through NCBI, CNKI, and Google Scholar with a combination of keywords, such as “GM”, “China”, “attitude”, “knowledge”, and “willingness” both in English and Chinese. Studies regarding the average knowledge level, acceptance and willingness-to-pay (WTP) for GM food among Chinese consumers, and factors affecting the WTP were included. Results: Of 1032 papers identified, 9 articles fulfilling the selection criteria were included in this systematic review. Among the 9 articles, 7 were written in English, 2 in Chinese. Awareness and knowledge of GM food among Chinese consumers were still not satisfying. Given the potential risks, the public tended to hold divergent attitudes to GM food, which had significantly influenced the WTP. Socio-economic factors such as the number of children, and external factors such as information and price also affected the WTP. Among all the factors, positive attitudes (including willingness-to-accept WTA), and positive information about GM food have significant positive influence on the WTP of GM food, while consumer‘s number of children, price of GM food and negative information about GM food would reduce the purchase intention. Discussion: Positive attitudes (including willingness-to-accept WTA), and positive information have significant positive influence on the WTP of GM food, while increasing number of children and price of GM food and negative information would reduce the purchase intention. It is necessary to strengthen the comprehensive and objective propaganda of GM food and transgenic technology; enhance the management of GM food; plan and develop GM food industry with focus on low-income consumers for they are more willing to buy GM food. / published_or_final_version / Public Health / Master / Master of Public Health

This item was digitized as part of the Million Books Project led by Carnegie Mellon University and supported by grants from the National Science Foundation (NSF). Cornell University coordinated the participation of land-grant and agricultural libraries in providing historical agricultural information for the digitization project; the University of Arizona Libraries, the College of Agriculture and Life Sciences, and the Office of Arid Lands Studies collaborated in the selection and provision of material for the digitization project.

11 pp. / The US Department of Agriculture (USDA) reported that over two million Americans are using USDA’s SuperTracker (Kevin Concannon, Under Secretary for Food, Nutrition and Consumer Services; March 27, 2013). SuperTracker is an online, interactive tool with which users can create customized healthy dietary and physical activity plans. It can help users analyze their dietary trends and assess whether they are achieving their dietary and physical activity goals. In addition to users’ planning, assessment, and analysis, SuperTracker offers personal goal setting and journaling – recording daily events. This article walks you through the basics of SuperTracker.

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