Thesis (Ph. D. in Cancer Biology)--Vanderbilt University, May 2008. / Title from title screen. Includes bibliographical references.

Thesis (Ph. D.)--University of Missouri-Columbia, 2000. / Typescript. Vita. Includes bibliographical references (leaves 150-177). Also available on the Internet.

Thesis (Ph.D. in Epidemiology) -- University of Colorado at Denver and Health Sciences Center, 2005. / Typescript. Includes bibliographical references (leaves 114-126).

Thesis (Ph. D.)--University of Missouri-Columbia, 2000. / Typescript. Vita. Includes bibliographical references (leaves 150-177). Also available on the Internet.

Bilinguals have been posited to have, compared to monolinguals, enhanced cognitive control, consequently exhibiting greater cognitive reserve, which is thought to subsequently delay the onset of clinical expression of dementia. Based on recent evidence suggesting that the more languages one manages the greater cognitive reserve, and that trilinguals undergo greater exercise in language control than bilinguals, this thesis investigated the effects of trilingualism and ageing on cognitive control, in young adults to older adults. As the thesis investigated the novel field of trilingualism and cognitive control, task complexity, the age of second and third language acquisition, language use, and physical and cognitive activity were also, importantly, assessed, as these are possible influencing factors in test performance. The participants completed several cognitive tasks; namely the Simon task, the Inhibition of return task, the Stroop task (inhibition) and the N-back task (working memory). The novel discovery of a trilingual (and bilingual) disadvantage was observed, which could explain some previous inconsistent findings in the bilingualism literature, where trilingualism may influence bilinguals’ test performance, as trilinguals and multilinguals are often mixed in with the bilingual group. Furthermore, the results suggest that second language acquisition and language use does not consistently predict performance in trilinguals (and bilinguals), nor does cognitive activity, although physical activity may modulate language group differences. Importantly, the results from this novel investigation of the effects of trilingualism and ageing on cognitive control suggest that trilingualism (and bilingualism) can, in some cases, be detrimental to cognitive control.

Bilinguals have been posited to have, compared to monolinguals, enhanced cognitive control, consequently exhibiting greater cognitive reserve, which is thought to subsequently delay the onset of clinical expression of dementia. Based on recent evidence suggesting that the more languages one manages the greater cognitive reserve, and that trilinguals undergo greater exercise in language control than bilinguals, this thesis investigated the effects of trilingualism and ageing on cognitive control, in young adults to older adults. As the thesis investigated the novel field of trilingualism and cognitive control, task complexity, the age of second and third language acquisition, language use, and physical and cognitive activity were also, importantly, assessed, as these are possible influencing factors in test performance. The participants completed several cognitive tasks; namely the Simon task, the Inhibition of return task, the Stroop task (inhibition) and the N-back task (working memory). The novel discovery of a trilingual (and bilingual) disadvantage was observed, which could explain some previous inconsistent findings in the bilingualism literature, where trilingualism may influence bilinguals’ test performance, as trilinguals and multilinguals are often mixed in with the bilingual group. Furthermore, the results suggest that second language acquisition and language use does not consistently predict performance in trilinguals (and bilinguals), nor does cognitive activity, although physical activity may modulate language group differences. Importantly, the results from this novel investigation of the effects of trilingualism and ageing on cognitive control suggest that trilingualism (and bilingualism) can, in some cases, be detrimental to cognitive control.

Malaria is a major public health problem in under developed countries especially countries undergoing war or conflict due to breakdown of health system and exposure of the vulnerable population through displacement. The knowledge, practice and attitude (KAP) of community members have direct influences on malaria preventive measures. A quantitative, explorative and descriptive study was conducted among rural communities of Mundri East County using interview schedules to ascertain malaria related knowledge, practices and attitude and the common factors hindering the malaria control measures at house hold level. Data were collected from 68 respondents from the randomly selected household through personal interviews using a pre-tested interview schedule which was analysed using the SPSS version 15 computer soft ware program. Most of the respondents demonstrated some understanding of malaria as a disease, its transmission, prevention and treatment, although there is a need to improve this through the preferable and acceptable community channels as well as the methods of accessing the modern and scientifically proved and acceptable methods. Given the relatively moderate acceptability of malaria control measures used by the participants in Mundri East County, there is need to conduct further research on the practical implemented measure including the local herbs used for preventive and treatment of malaria Understanding the factors that inhibits or promotes the malaria control measures at community level is necessary for better planning and implementation of malaria intervention programme, which keeps complex and sensitive matters such as educational background, religious and cultural beliefs, and political affiliations in mind. / Health Studies / M.A. (Public Health)

Bovine tuberculosis (TB) is a contagious neglected zoonosis of cattle that is prevalent but under-investigated in Cameroon, hence this study was designed to assess the epidemiology of bovine TB in cattle, risks for M. bovis infection in cattle and humans; and public health implications of zoonotic bovine TB in the highlands of Cameroon. A retrospective study of meat inspection records (1994 – 2010) was done to estimate the prevalence of TB lesions in slaughtered cattle in the North West region. The prevalence of bovine TB and anti-bovine TB antibodies in live cattle based on tuberculin skin tests (2 surveys) and immune-chromatographic assays respectively were carried out in the Western and Adamawa highlands of Cameroon. The performance of the tuberculin tests for bovine TB diagnosis in cattle using various tuberculin skin test cut-off points against the detection of anti-bovine TB antibodies (hypothesised risks of exposure) was compared. Suspected TB lesions from slaughtered cattle and infected human sputa were cultured on Lowentein – Jesen and Middlebrook 7H9 media to isolate mycobacteria agents for molecular genotyping using genomic deletion analysis and spoligotyping. Risk factors for exposure and transmission of zoonotic bovine TB infection of cattle and cattle professionals, and its public health significance were determined using structured questionnaires. Seventeen years of meat inspection record revealed that suspect TB lesions were identified in 599 of 129,165 slaughtered cattle at the Bamenda abattoir. The lungs and associated lymph nodes (over 60%) were the most affected tissues. Other results showed that the prevalence of anti-bovine TB antibodies in cattle in the study regions was 37.17%. Chi square statistics revealed that irrespective of the tuberculin test cut-off value (P<0.05; χ2>48), strong associations existed between the detection of anti-bovine TB antibodies and disease status. A 95% confidence interval analysis of the comparative cervical tuberculin tests revealed that the prevalence rates were 4.67% – 7.15%, 12.02% – 15.67% and 20.56% – 24.98% at the ≥ 4mm, ≥ 3mm and ≥ 2mm cut-off points, respectively. Overall, the best test performance was realised at ≥ 3-mm, though the ≥ 2-mm cut-off point predicted more positive reactors. Age, sex, breed and husbandry practices served as significant (P<0.05) risks to the prevalence and exposure of bovine TB in cattle. The feedbacks from cattle professionals suggested that there was high possibility of cattle to cattle and cattle to human transmission of bovine TB such as intimate and repeated animal / animal and animal / human interactions, consuming unpasteurised milk and eating raw meat. Genomic deletion analysis of cultured isolates showed evidence of M. tuberculosis from cattle and M. bovis from human while spoligotyping identified five cattle M. bovis strains; and four spoligotype patterns that had not been previously described anywhere. The study has important epidemiological and public health implications requiring prompt and decisive actions from the Cameroonian authority towards controlling zoonotic bovine TB in both humans and animals. A multidisciplinary approach is needed for further collaborative research and effective control strategies such as enhancing the awareness of people to this deadly disease through continuous education, proper food handling and personal hygiene, healthy husbandry practices and maintenance of the environment.

Malaria is a major public health problem in under developed countries especially countries undergoing war or conflict due to breakdown of health system and exposure of the vulnerable population through displacement. The knowledge, practice and attitude (KAP) of community members have direct influences on malaria preventive measures. A quantitative, explorative and descriptive study was conducted among rural communities of Mundri East County using interview schedules to ascertain malaria related knowledge, practices and attitude and the common factors hindering the malaria control measures at house hold level. Data were collected from 68 respondents from the randomly selected household through personal interviews using a pre-tested interview schedule which was analysed using the SPSS version 15 computer soft ware program. Most of the respondents demonstrated some understanding of malaria as a disease, its transmission, prevention and treatment, although there is a need to improve this through the preferable and acceptable community channels as well as the methods of accessing the modern and scientifically proved and acceptable methods. Given the relatively moderate acceptability of malaria control measures used by the participants in Mundri East County, there is need to conduct further research on the practical implemented measure including the local herbs used for preventive and treatment of malaria Understanding the factors that inhibits or promotes the malaria control measures at community level is necessary for better planning and implementation of malaria intervention programme, which keeps complex and sensitive matters such as educational background, religious and cultural beliefs, and political affiliations in mind. / Health Studies / M.A. (Public Health)

Thesis (M.Sc. (Soil Science)) -- University of Limpopo, 2022 / In South Africa, land use planning has received limited attention in areas perceived as suitable for agricultural production. In the lack of reliable soil type and fertility status information, crop yields remain lower than the land’s potential, with subsequent land degradation. Despite this, studies that focused on land capability and soil suitability to date have not considered the spatial variability of the soil nutrients and factors influencing their variability. However, this information is key for site-specific soil management. Therefore, it is vital to link land capability and soi suitability with the spatial variability of soil nutrients as it opens opportunities for more rational management of the soil resources since soil nutrients directly affect crop growth and consequently yield. To address this issue, a study was conducted on a 12 ha banana plantation portion of the Makuleke farm. The main objectives of this study were to (1) survey, classify and characterise soils in order to derive and map land capability classes of Makuleke farm, (2) quantify the physical and chemical properties of the soils in order to derive and map the soil suitability of Makuleke farm for banana production, (3) assess the spatial variability and structure of soil nutrients across the Makuleke farm and (4) Identify the factors of control of the spatial variability of the soil nutrients across the Makuleke farm. To begin with, a field soil survey was conducted using transect walks complemented by auger observations to sub-divide the 12 ha banana plantation portion of the farm into varied soil mapping units. Thereafter, soil classification was done to group soils based on their morphological properties and pedological processes. During soil classification, a total of 12 representative profile pits (1.5 m × 1.5 m long × 2 m deep/limiting layer) were excavated, studied, described, and sampled. At each profile pit, three replicates samples were collected at 0 – 30 cm depth intervals giving rise to 36 bulk soil samples. From the gathered soil profile information, four soil units were thus delineated and identified across the 12 ha banana plantation. For soil fertility assessment, a grid sampling strategy at 50 × 50 m was adopted to collect the samples across the 12 ha banana plantation. A total of 27 composite samples were collected at the nodes of the grid, and thereafter bagged, labelled, and transported to the laboratory. In the laboratory, all collected samples were air-dried and sieved using a 2 mm sieve in preparation for soil physical and chemical properties analysis. The land capability assessment of Makuleke farm was done using the concepts and principles of the FAO framework for Land Evaluation (FAO, 1976), but adapted to South African conditions by Smith (2006). Soil suitability assessment was done using the FAO framework for Land Evaluation (FAO, 1976) coupled with the guidelines for rainfed agriculture (FAO, 1983) and the criteria proposed by Sys et al. (1993) and Naidu et al. (2006). To assess the spatial variability and structure of the soil nutrients across the farm, classical and geostatistical techniques were employed respectively. A correlation matrix was employed to identify key factors influencing the spatial variability of soil nutrients across the farm. For interpolation, ordinary kriging was used to generate soil nutrient spatial distribution maps. In this study, four soil forms were identified and classified as Hutton, Westleigh, Glenrosa, and Valsrivier, which are broadly distinguished as Lixisols, Plinthosols, Leptosols, and Cambisols. Land capability results revealed that 17% of the 12 ha portion of the farm has very high arable potential (I), 60% of the farm has medium arable potential (III), 6% has low arable potential (IV) and 17 % is non-arable (VI), which might explain the varied banana yields in the farm. Soil suitability analysis revealed that 12% of the 12 ha farm is highly suitable (S1), 34% is moderately suitable (S2), 38% is marginally suitable (S3) and 16% is permanently not suitable (N2) for banana production. The low arable and marginally suitable portion of the farm was under Valsrivier soils which were limited by its shallow depth, shallow rooting depth, acidic soil pH, low organic carbon (OC), and the fact that it was located on a steeper slope gradient. The non-arable and not suitable portion of the farm for banana production was under Glenrosa and it was limited by its location on a steep slope gradient and was characterised by shallow effective rooting depth, low OC, low clay content, and acidic soil pH. Classical statistical techniques revealed that phosphorus (P), potassium (K), calcium (Ca), zinc (Zn), manganese (Mn), and copper (Cu) content varied highly across the banana plantation, while magnesium (Mg) and total nitrogen (TN) varied moderately. In addition, the geostatistical analysis revealed that spatial dependency was weak (Ca, Cu, and TN), moderate (Mg and Zn), and strong (P, K, and Mn) for the different soil nutrients across the 12 ha banana plantation. Soil nutrients with strong spatial dependency have a good spatial structure and are easily manageable (in terms of fertilisation, liming, and irrigation) across the farm compared to the ones with weak spatial dependency which have a poor structure. This study also found that land attributes, which are soil type and topographic position were the main factors driving the spatial variability of the soil nutrients across the farm. In terms of soil type, soils such as Valsrivier and Glenrosa with 2:1 clay-type smectite were the ones that had nutrient content compared to soils with 1:1 clay-type kaolinite (e.g., Westleigh and Hutton). Higher nutrient contents were also observed in the footslope position compared to the middleslope of the farmland. Correlation analysis revealed that Mn was the key polyvalent cation influencing the spatial variability of P, K, and Zn. Soil pH and effective cation exchanges capacity (ECEC) were the key soil factors driving the spatial variability of Ca, while ECEC was the key factor affecting the spatial variability of Mg. Moreover, the spatial variability of soil Mn and Cu was driven by soil Cu and clay content, respectively. The kriged maps showed that P, Mg, Zn, and Mn were high in the northeast part and low in the northwest part of the farm. Similarly, K and Ca were low in the northwest part, but they were high in the south to the southwest part of the study area. Total nitrogen was high in the west part and low in the east-northeast part, while Cu was evenly distributed across the plantation. This study highlights the importance of prior land use planning (i.e., land capability and soil suitability) and fertility assessment for agricultural production. The research results obtained provide the actual reference state of the capability of the land for arable farming and soil suitability for banana production at Makuleke farm. Moreover, the research results provide the spatial variability and structure of the soil nutrients which have a greater impact on the growth and yield of bananas. The results obtained in this study will be useful for site-specific management of soil nutrients and other soil management practices (e.g., irrigation, fertilisation, liming, etc.), developing appropriate land use plans, and quantifying anthropogenic impacts on the soil system and thus improving land productivity. / National Research Foundation (NRF)