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The pathogenic subgroup C feline leukemia virus (C virus) arises in infected cats by mutations in the envelope gene (env) of subgroup A FeLV (A virus) that switches the host receptor used for infection. To better understand C virus emergence and potential FeLV variants that may arise, I characterized FeLV Env sequence isolated from the primary FY981 FeLV isolate derived from an anemic cat. I show that the FY981 pseudotype virus is capable of using both the A virus receptor THTR1, and the C virus receptor FLVCR1 for infection, consistent with the FY981 Env being a hybrid A virus/C virus Env. Furthermore, I propose that pathogenic C virus arise in infected cats through FeLV variants or intermediates that are multi-tropic in their receptor use.

The pathogenic subgroup C feline leukemia virus (C virus) arises in infected cats by mutations in the envelope gene (env) of subgroup A FeLV (A virus) that switches the host receptor used for infection. To better understand C virus emergence and potential FeLV variants that may arise, I characterized FeLV Env sequence isolated from the primary FY981 FeLV isolate derived from an anemic cat. I show that the FY981 pseudotype virus is capable of using both the A virus receptor THTR1, and the C virus receptor FLVCR1 for infection, consistent with the FY981 Env being a hybrid A virus/C virus Env. Furthermore, I propose that pathogenic C virus arise in infected cats through FeLV variants or intermediates that are multi-tropic in their receptor use.

Amostras de sangue total e suabes de orofaringe e conjuntiva foram coletadas de 57 felídeos Neotropicais (1 Leopardus geoffroyi, 17 L. wiedii, 22 L. tigrinus, 14 L. pardalis e 3 Puma yagouaroundi) mantidos em cativeiro no Refúgio Bela Vista, Foz do Iguaçu. Dados clínicos, hemograma e histórico dos animais foram disponibilizados. Materiais clínicos obtidos a partir dos suabes de orofaringe e conjuntiva foram submetidos ao cultivo para Mycoplasma spp em meio específico. DNA do sangue e suabes foram extraídos por meio de um kit comercial e pelo método de fervura, respectivamente. DNA extraído de amostras de sangue foram submetidos à PCR para detecção de Mycoplasma haemofelis (Mhf), Candidatus M. haemominutum (CMhm), DNA proviral do vírus da leucemia felina (FeLV) e Bartonella spp. DNA extraído dos suabes foram submetidos à PCR para detecção de Mollicutes e M. felis. Foi realizada uma análise de associação entre alterações clínicas e a infecção por Bartonella spp e um estudo de fator de risco da infecção por esse microrganismo. Apenas 1 (1,75%) animal foi positivo a reação para Mhf e nenhum foi positivo a reação para CMhm. Dois (3,5%) animais foram positivos a reação para FeLV e 6 (10,52%) foram positivos para Bartonella spp. Não houve co-infecção entre os agentes pesquisados nas amostras de sangue. Foram obtidos 5 (8,77%) isolados de Mycoplasma spp da orofaringe e nenhum de conjuntiva. DNA de Mollicutes foi detectado em 53 (93%) e 27 (47,36%) amostras de orofaringe e conjuntiva, respectivamente. Nenhuma amostra apresentou resultado positivo na detecção de DNA alvo de M. felis. Não houve associação entre as alterações hematológicas (anemia, desidratação, leucocitose, leucopenia, histórico de anemia) e a infecção por Bartonella spp. Machos apresentam maior risco de adquirir bartonelose do que fêmeas. Este é o primeiro relato da presença de DNA proviral de FeLV em L. tigrinus e L. pardalis no sul do aís, de DNA de B. henselae em L. tigrinus, L. pardalis, L. geoffroyii e P. yagouaroundi, e de um estudo de fator de risco associado a infecção por Bartonella spp em felídeos neotropicais. / Total blood samples and oropharinx and conjunctival swabs were collected from 57 neotropical felids (1 Leopardus geoffroyi, 17 L. wiedii, 22 L. tigrinus, 14 L. pardalis and 3 Puma yagouaroundi) maintained in captivity at Refúgio Bela Vista, Foz do Iguaçu. Clinical data, hemogram and clinical history of these animals were available. Clinical materials obtained from oropharinx and conjunctiva were cultured in specific media for Mycoplasma spp isolation. DNA of blood and swabs were extracted using a commercially available kit and a boiling method, respectively. DNA samples from swabs were submitted to a PCR for the detection of Mollicutes and M. felis. DNA samples from blood were submitted to a PCR for detection of Mycoplasma haemofelis (Mhf), Candidatus M. haemominutum (CMhm), feline leukemia virus (FeLV) proviral DNA , and Bartonella spp. The association between hematological alterations and bartonella infection was evaluated and a risk factor analysis was performed. Only 1 (1.75%) animal was positive for Mhf reaction, whereas all animals were negative for CMhm detection. Two (3.5%) animals were positives for FeLV and 6 (10.52%) animals were positive for Bartonella spp, by PCR. Co-infections among these agents were not observed. Five (8.77%) mycoplasma isolates were obtained from oropharinx samples and none was obtained from conjunctival samples. Mollicutes DNA was detected in 53 (93%) and 27 (47.36%) samples from oropharinx and conjunctiva, respectively. All samples were negative for M. felis detection. Hematological alterations (anemia, dehydration, leukocytosis, leucopenia, history of anemia) were not associated to Bartonella spp infection. Males are more likely to be infected than females. This is the first report of FeLV proviral DNA in L. tigrinus and L. pardalis in Southern Brazil, of B. henselae DNA in L. trigrinus, L. pardalis, L. geoffroyi and P. yagouaroundi, and the first study of risk factors for Bartonella spp infection in neotropical felids.

Amostras de sangue total e suabes de orofaringe e conjuntiva foram coletadas de 57 felídeos Neotropicais (1 Leopardus geoffroyi, 17 L. wiedii, 22 L. tigrinus, 14 L. pardalis e 3 Puma yagouaroundi) mantidos em cativeiro no Refúgio Bela Vista, Foz do Iguaçu. Dados clínicos, hemograma e histórico dos animais foram disponibilizados. Materiais clínicos obtidos a partir dos suabes de orofaringe e conjuntiva foram submetidos ao cultivo para Mycoplasma spp em meio específico. DNA do sangue e suabes foram extraídos por meio de um kit comercial e pelo método de fervura, respectivamente. DNA extraído de amostras de sangue foram submetidos à PCR para detecção de Mycoplasma haemofelis (Mhf), Candidatus M. haemominutum (CMhm), DNA proviral do vírus da leucemia felina (FeLV) e Bartonella spp. DNA extraído dos suabes foram submetidos à PCR para detecção de Mollicutes e M. felis. Foi realizada uma análise de associação entre alterações clínicas e a infecção por Bartonella spp e um estudo de fator de risco da infecção por esse microrganismo. Apenas 1 (1,75%) animal foi positivo a reação para Mhf e nenhum foi positivo a reação para CMhm. Dois (3,5%) animais foram positivos a reação para FeLV e 6 (10,52%) foram positivos para Bartonella spp. Não houve co-infecção entre os agentes pesquisados nas amostras de sangue. Foram obtidos 5 (8,77%) isolados de Mycoplasma spp da orofaringe e nenhum de conjuntiva. DNA de Mollicutes foi detectado em 53 (93%) e 27 (47,36%) amostras de orofaringe e conjuntiva, respectivamente. Nenhuma amostra apresentou resultado positivo na detecção de DNA alvo de M. felis. Não houve associação entre as alterações hematológicas (anemia, desidratação, leucocitose, leucopenia, histórico de anemia) e a infecção por Bartonella spp. Machos apresentam maior risco de adquirir bartonelose do que fêmeas. Este é o primeiro relato da presença de DNA proviral de FeLV em L. tigrinus e L. pardalis no sul do aís, de DNA de B. henselae em L. tigrinus, L. pardalis, L. geoffroyii e P. yagouaroundi, e de um estudo de fator de risco associado a infecção por Bartonella spp em felídeos neotropicais. / Total blood samples and oropharinx and conjunctival swabs were collected from 57 neotropical felids (1 Leopardus geoffroyi, 17 L. wiedii, 22 L. tigrinus, 14 L. pardalis and 3 Puma yagouaroundi) maintained in captivity at Refúgio Bela Vista, Foz do Iguaçu. Clinical data, hemogram and clinical history of these animals were available. Clinical materials obtained from oropharinx and conjunctiva were cultured in specific media for Mycoplasma spp isolation. DNA of blood and swabs were extracted using a commercially available kit and a boiling method, respectively. DNA samples from swabs were submitted to a PCR for the detection of Mollicutes and M. felis. DNA samples from blood were submitted to a PCR for detection of Mycoplasma haemofelis (Mhf), Candidatus M. haemominutum (CMhm), feline leukemia virus (FeLV) proviral DNA , and Bartonella spp. The association between hematological alterations and bartonella infection was evaluated and a risk factor analysis was performed. Only 1 (1.75%) animal was positive for Mhf reaction, whereas all animals were negative for CMhm detection. Two (3.5%) animals were positives for FeLV and 6 (10.52%) animals were positive for Bartonella spp, by PCR. Co-infections among these agents were not observed. Five (8.77%) mycoplasma isolates were obtained from oropharinx samples and none was obtained from conjunctival samples. Mollicutes DNA was detected in 53 (93%) and 27 (47.36%) samples from oropharinx and conjunctiva, respectively. All samples were negative for M. felis detection. Hematological alterations (anemia, dehydration, leukocytosis, leucopenia, history of anemia) were not associated to Bartonella spp infection. Males are more likely to be infected than females. This is the first report of FeLV proviral DNA in L. tigrinus and L. pardalis in Southern Brazil, of B. henselae DNA in L. trigrinus, L. pardalis, L. geoffroyi and P. yagouaroundi, and the first study of risk factors for Bartonella spp infection in neotropical felids.

<p>&nbsp;Иако су 60&ndash;тих година прошлога века по први пут описане, леукемија мачке (FeLV; engl. Feline leukemia virus) и мачји заразни перитонитис (FIP; engl. Feline infectious peritonitis) који је узрокован мачјим коронавирусом (FCoV; engl. Feline Coronavirus), те 80&ndash;тих година прошлога века први пут инфекција вирусом мачје имунодефицијенције (FIV; engl. Feline Immunodeficiency Virus), те три болести су до данас остале неизлечиве, смртоносне, а леукемија и имунодефицијенција су повезане и с настајањем малигних оболења код домаћих и дивљих мачака. Многа питања везана уз епидемиологију и превенцију остала су до сада неразјашњена. Стога мачји коронавирус (FCoV), вирус мачје леукемије (FeLV) и вирус мачје имунодефицијенције (FIV) заузимају важно место у истраживањима и у ветеринарској свакодневној пракси. Главни епидемиолошки и превентивни проблем код свих трију зараза представљају инфициране мачке које не показују клиничке знаке болести или имају неодређене клиничке знаке, а шире инфекције путем пљувачке, урина, фецеса, суза, крви и осталих телесних течности. Тако се инфекција вирусом мачје леукемије (FeLV) и вирусом мачје имунодефицијенције (FIV) споро развија код мачака, па мачка у раздобљу обично дужем од четири године не показује клиничке знаке или има неодређене клиничке знаке, а представља стални извор заразе и шири инфекцију унутар мачје популације. Виремичне мачке представљају велику опасност за осталу мачју популацију посебно у домаћинствима с више мачака, ако иду напоље, у одгајивачницама, склоништима за мачке, ветеринарским амбулантама, клиникама и у ветеринарској трансфузиологији. Главни превентивни проблем код вируса мачје леукемије, мачјег заразног перитонитиса и вируса мачје имунодефицијенције је непостојање на тржишту комерцијално делотворне вакцине. Будући да су досадашња епидемиолошка истраживања показала да FCoV, FeLV и FIV показују велику разлику у вируленцији унутар различитих мачјих популација и да разлика у вируленцији зависи од географског положаја, начина држања и о пасмини, истраживања треба спровести за сваку поједину земљу. Циљеви овога епидемиолошког истраживања су били следећи:</p><p>&bull; одређивање преваленције инфекција и коинфекција узрокованих коронавирусима мачака (FCoV), вирусима леукемије (FeLV) и имунодефицијенције (FIV) на подручју Загреба и Вараждина;<br />&bull; одређивање епидемиолошких фактора ризика и њихова значаја за настанак, развој и ширење FCoV, FeLV и FIV у популацији домаћих мачака.<br />Ово епидемиолошко истраживање је спроведено на 106 мачака. Лабораторијски тестови вирусолошких показатеља су рађени на Ветеринарском факултету у Бечу. Узорци су претражени на FCoV антитела тестом имунофлуоресцентних антитијела (IFA), FeLV-п27 антиген помоћу имуноензимског теста (ELISA), FeLV специфичне нуклеинске киселине користећи real-time PCR (Applied Biosystem 7300 Real time PCR System) и FIV антитела са брзим имуномиграцијским тестом (RIM). Резултати лабораторијских претрага вирусолошких показатеља показали су да је:<br />&bull; 45,28% тестираних мачака било позитивно на FCoV инфекцију;<br />&bull; 6,60% тестираних мачака било позитивно на FeLV инфекцију;<br />&bull; 7,55% тестираних мачака имало коинфекцију са FeLV и FCoV;<br />&bull; 5,66% тестираних мачака имало коинфекцију са FIV и FCoV.<br />Резултати епидемиолошких показатеља показали су да је доказана:<br />&bull; статистички значајна асоцијација између здравственог статуса и групе FeLV+;<br />&bull; статистички значајна асоцијација између излажења напоље без власникове контроле и група FCoV+ и FIV/FCoV+.<br />Одређивање учесталости FCoV, FeLV и FIV уз факторе ризика за настанак, развој и ширење зараза је од великог значаја за спречавање ширења ова три вируса унутар популације мачака на градским подручјима.</p> / <p>&nbsp;Iako su 60&ndash;tih godina prošloga veka po prvi put opisane, leukemija mačke (FeLV; engl. Feline leukemia virus) i mačji zarazni peritonitis (FIP; engl. Feline infectious peritonitis) koji je uzrokovan mačjim koronavirusom (FCoV; engl. Feline Coronavirus), te 80&ndash;tih godina prošloga veka prvi put infekcija virusom mačje imunodeficijencije (FIV; engl. Feline Immunodeficiency Virus), te tri bolesti su do danas ostale neizlečive, smrtonosne, a leukemija i imunodeficijencija su povezane i s nastajanjem malignih obolenja kod domaćih i divljih mačaka. Mnoga pitanja vezana uz epidemiologiju i prevenciju ostala su do sada nerazjašnjena. Stoga mačji koronavirus (FCoV), virus mačje leukemije (FeLV) i virus mačje imunodeficijencije (FIV) zauzimaju važno mesto u istraživanjima i u veterinarskoj svakodnevnoj praksi. Glavni epidemiološki i preventivni problem kod svih triju zaraza predstavljaju inficirane mačke koje ne pokazuju kliničke znake bolesti ili imaju neodređene kliničke znake, a šire infekcije putem pljuvačke, urina, fecesa, suza, krvi i ostalih telesnih tečnosti. Tako se infekcija virusom mačje leukemije (FeLV) i virusom mačje imunodeficijencije (FIV) sporo razvija kod mačaka, pa mačka u razdoblju obično dužem od četiri godine ne pokazuje kliničke znake ili ima neodređene kliničke znake, a predstavlja stalni izvor zaraze i širi infekciju unutar mačje populacije. Viremične mačke predstavljaju veliku opasnost za ostalu mačju populaciju posebno u domaćinstvima s više mačaka, ako idu napolje, u odgajivačnicama, skloništima za mačke, veterinarskim ambulantama, klinikama i u veterinarskoj transfuziologiji. Glavni preventivni problem kod virusa mačje leukemije, mačjeg zaraznog peritonitisa i virusa mačje imunodeficijencije je nepostojanje na tržištu komercijalno delotvorne vakcine. Budući da su dosadašnja epidemiološka istraživanja pokazala da FCoV, FeLV i FIV pokazuju veliku razliku u virulenciji unutar različitih mačjih populacija i da razlika u virulenciji zavisi od geografskog položaja, načina držanja i o pasmini, istraživanja treba sprovesti za svaku pojedinu zemlju. Ciljevi ovoga epidemiološkog istraživanja su bili sledeći:</p><p>&bull; određivanje prevalencije infekcija i koinfekcija uzrokovanih koronavirusima mačaka (FCoV), virusima leukemije (FeLV) i imunodeficijencije (FIV) na području Zagreba i Varaždina;<br />&bull; određivanje epidemioloških faktora rizika i njihova značaja za nastanak, razvoj i širenje FCoV, FeLV i FIV u populaciji domaćih mačaka.<br />Ovo epidemiološko istraživanje je sprovedeno na 106 mačaka. Laboratorijski testovi virusoloških pokazatelja su rađeni na Veterinarskom fakultetu u Beču. Uzorci su pretraženi na FCoV antitela testom imunofluorescentnih antitijela (IFA), FeLV-p27 antigen pomoću imunoenzimskog testa (ELISA), FeLV specifične nukleinske kiseline koristeći real-time PCR (Applied Biosystem 7300 Real time PCR System) i FIV antitela sa brzim imunomigracijskim testom (RIM). Rezultati laboratorijskih pretraga virusoloških pokazatelja pokazali su da je:<br />&bull; 45,28% testiranih mačaka bilo pozitivno na FCoV infekciju;<br />&bull; 6,60% testiranih mačaka bilo pozitivno na FeLV infekciju;<br />&bull; 7,55% testiranih mačaka imalo koinfekciju sa FeLV i FCoV;<br />&bull; 5,66% testiranih mačaka imalo koinfekciju sa FIV i FCoV.<br />Rezultati epidemioloških pokazatelja pokazali su da je dokazana:<br />&bull; statistički značajna asocijacija između zdravstvenog statusa i grupe FeLV+;<br />&bull; statistički značajna asocijacija između izlaženja napolje bez vlasnikove kontrole i grupa FCoV+ i FIV/FCoV+.<br />Određivanje učestalosti FCoV, FeLV i FIV uz faktore rizika za nastanak, razvoj i širenje zaraza je od velikog značaja za sprečavanje širenja ova tri virusa unutar populacije mačaka na gradskim područjima.</p> / <p>Although in the nineteen sixties feline leukemia virus (FeLV) and feline infectious peritonitis (FIP) induced by feline coronavirus (FCoV) were described for the first time and feline immunodeficiency virus (FIV) was described in the nineteen eighties for the first time, too, all three diseases have been untreatable and lethal to the present day. Feline leukemia virus and feline immunodeficiency virus are associated with development of neoplasm diseases in domestic and wild cats. Many questions associated with epidemiology and prevention have not still been clarified. Feline coronavirus (FCoV), feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) play an important role in investigations and everyday veterinary practice.<br />The main epidemiologic and preventive problems in all three diseases are infected cats. They have no clinical signs of disease or have undefined clinical symptoms but they spread infections by saliva, urine, feces, tears, blood and other body fluids among the cat population. Infections of feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) develop slowly in cats. Viremic cat during a period longer than four years does not show clinical signs or has undefined symptoms and is constant source of infection and spreads infection in cat population. Viremic cats are great danger for cat populations especially in multiple cat households, if they go outside, in catteries, cat shelters, veterinary clinics or blood<br />transfusions. The main preventive problem with feline leukemia virus, feline infectious peritonitis and feline immunodeficiency virus is that there is no effective vaccine in the market. As to date epidemiologic research has shown that FCoV, FeLV and FIV have shown a great difference in virulence in various cat populations and as the virulence varies according to geographic location, maintenance and breeding research should be carried out for each particular country. The aims in this epidemiologic research were the following:<br />&bull; to determine the prevalence of infections and co-infections induced by feline coronaviruses (FCoV), feline leukemia virus (FeLV) and feline immunodeficiency virus (FIV) in the areas of Zagreb and Varaždin;<br />&bull; to determine which epidemiologic factors are risky and their significance for the occurrence, development and spreading of FCoV, FeLV and FIV in population of domestic cats.<br />This epidemiologic research was carried out on 106 cats. Laboratory tests of viral parameters were carried out at the Faculty of Veterinary Medicine in Vienna. The samples were investigated for the presence of FCoV antibodies by Immunofluorescence Antibody Assay (IFA), for FeLV-p27 antigen by enzyme linked immunosorbent assay (ELISA), for FeLV specific nucleic acids by real-time PCR (Applied Biosystems 7300 Real time PCR System) and for the presence of FIV antibodies by rapid immunomigration test (RIM). Lab tests results of viral parameters demonstrated that:<br />&bull; 45.28% tested cats were positive for FCoV infection;<br />&bull; 6.60% tested cats were positive for FeLV infection;<br />&bull; 7.55% tested cats were co-infected with FeLV and FCoV;<br />&bull; 5.66% tested cats were co-infected with FIV and FCoV.<br />The results of epidemiologic parameters demonstrated that:<br />&bull; statistically significant association was found in health status and group FeLV+;<br />&bull; statistically significant association was found in outdoor access without owner&#39;s control and groups FCoV+ and FIV/FCoV+.<br />Determining FCoV, FeLV and FIV prevalence with risk factors for the occurrence, development and spread of infections is of great importance to prevent the spread of these three viruses in cat population in urban areas.</p>

Hematopoietic tumors are the most common neoplastic disorders in felines. Many of these tumors are associated with infection by feline leukemia virus (FeLV) and one of the described mechanisms is the integration of viral material into the feline genome, which can lead to genomic instability and consequent alteration of genes related to proliferation control and cell death. When larger DNA fragments are affected, such changes can be observed by cytogenetic analysis. The present study aimed to observe cytogenetic changes in felines with hematopoietic neoplasms. The study was performed in eight felines, seven of them with a diagnosis of leukemia or lymphoma. The control consisted of a healthy feline FeLV negative with normal karyotype. At least 10 metaphases of each animal were analyzed. Additionally, 1000 lymphocytes of these two patients, were analyzed and classified cytologically by their viability (necrosis, apoptosis), mitotic state (mononuclear, binucleate (BN), mitotic multinucleate) and their chromosomal damage or instability state (presence of micronucleus (MN) in mononuclear, binucleate, as well as nuclear buds (NBUD). The results of this observation were statistically analyzed by the Wilcoxon paired test. In this chromosomal analysis two of these animals presented monosomy of the E3 chromosome, one with diagnosis of acute myeloid leukemia of the M6a (LMA-M6a) FAB subtype and another with multicenter lymphoma (LM). There were significant differences in LMA-M6a scores (N = 7, Z = 2.36, p = 0.01) and LM scores (N = 8, Z = 2.52, p = 0.01) when group control. However, there was no difference between AML and lymphoma (N = 8, Z = 0.07, p = 0.94). The E3 chromosome has 1401 genes, several related to cell cycle control (Plk1, Sun, Mad1l1, Mcm7), DNA repair (Pms2, Usp42), tumor suppression (Bcl7b). The loss of DNA fragments, such as the loss of the E3 chromosome in the two patients described, led to the haploinsufficiency of important genes for the cell cycle, and this could be a cause of genomic instability and consequently susceptibility to the development of cancer. The observation of cytogenetic alterations, in this way, allows a better understanding of the cancer in the feline species and translational research. / Os tumores hematopoiéticos são os distúrbios neoplásicos mais comuns em felinos. Muitos desses tumores estão associados à infecção pelo vírus da leucemia felina (FeLV) e um dos mecanismos descritos é pela integração de material viral no genoma felino, que pode levar à instabilidade genômica e consequente alteração de genes relacionados com o controle da proliferação e morte celular. Quando fragmentos maiores de DNA são afetados, tais alterações podem ser observadas pela análise citogenética. O presente estudo objetivou observar as alterações citogenéticas em felinos com neoplasias hematopoiéticas. O estudo foi realizado em oito felinos, sete destes com diagnóstico de leucemia ou linfoma. O controle foi constituído por um felino saudável FeLV negativo com cariótipo normal. Foram analisadas no mínimo 10 metáfases de cada animal. Adicionalmente, destes dois pacientes foram analisados 1.000 linfócitos e classificados citologicamente pelo seu estado de viabilidade (necrose, apoptose), seu estado mitótico (mononucleado, binucleado (BN), multinucleado, mitótico) e seu dano cromossômico ou estado de instabilidade (presença de micronúcleo (MN) em célula mononucleada, binucleada, bem como brotos nucleares (NBUD). Os resultados dessa observação foram analisados estatisticamente pelo teste pareado de Wilcoxon. Nesta análise cromossômica dois destes animais apresentaram monossomia do cromossomo E3, um com diagnóstico de leucemia mieloide aguda do subtipo FAB M6a (LMA-M6a) e outro com linfoma multicêntrico (LM). Houve diferença significativa nas contagens de LMA-M6a (N=7; Z=2,36; p=0,01) e de LM (N=8; Z=2,52; p=0,01) quando comparados com o grupo controle. No entanto, não houve diferença entre LMA e linfoma (N=8; Z=0,07; p=0,94). No cromossomo E3 são descritos 1401 genes, sendo vários relacionados com controle de ciclo celular, (Plk1, Sun, Mad1l1, Mcm7), reparo de DNA (Pms2, Usp42) e supressão tumoral (Bcl7b).A perda de fragmentos de DNA, como o cromossomo E3 nos dois pacientes descritos, que leva a haploinsuficiência de genes importantes para o ciclo celular, poderia ser a causa de instabilidade genômica e, consequentemente suscetibilidade ao desenvolvimento do câncer. A observação de alterações citogenéticas, dessa forma, possibilita o melhor entendimento do câncer na espécie felina e serve como subsídio para a pesquisa translacional.