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Typescript (photocopy). / Digitized by Kansas Correctional Industries

This study was conducted to investigate the effects of varying levels of dietary lysine and feed allocation, on the performance of female broiler breeders. In trial 1, 900 Cobb breeders from 26 to 45 weeks of age were used. The first four treatments had a fixed level of lysine throughout the experimental period, with 1200, 1070, 930 and 800 mg Lysine/bird/day and the last two treatments had their levels of lysine changed every two weeks, with treatment five started off with 1200 mg lysine/bird/day followed by a gradual decrease up to 975 mg lysine/bird/day at 45 week old, while treatment six started with the lowest level of lysine, 800 mg/bird/day and at 45 week old the intake was 1025 mg/bird/day. The rations provided 1900KJ ME/ day, the birds received 160 g of feed/day. Dietary lysine did not affect body weight, egg production, or egg composition. There were no significant differences in age at 50%, or peak production. Birds receiving 1070 and 1025 mg lysine/bird.day had a slightly, but not significantly, higher production in the current study. Birds receiving 1200 mg lysine/bird.day had the highest body weight and the lowest egg production. In trial 2, 900 broiler breeder hens were used. Protein intake and feed allocation were changed for each treatment at 26, 38 and 50 weeks and ending at 60 weeks. Birds on T1 were fed a constant CP content (145 g protein/kg) but a decreasing feed allocation (175 to 145 g/d). Those on T2 had a constant feed allocation (160 g/d) and a decreasing CP (166 to 124 g/kg) while those on T3 had varying levels of protein (166, 124 and 166 g/kg) and feed allocation (160, 160, 145 g/d). Mean cumulative protein intakes were 5.2, 5.3 and 4.8 kg/bird and mean energy intakes were 417, 412 and 402 MJ/bird for T1, T2 and T3 respectively. Body weights differed significantly at the end of the trial (P<0.05) with T2 showing the highest weight gain. However, egg production, egg weight and egg output were not affected by treatment. These breeders did not benefit from the additional protein and energy provided in the first and second periods by T1 and T2 (vs. T3), nor did they benefit from the additional energy provided in the final ten weeks of production by T2. / M.Sc.Agric. University of KwaZulu-Natal, Pietermaritzburg 2013.

Physicochemical properties of different fibrous ingredients were used to determine the influence of feed bulk on voluntary feed intake and gut capacity in weaner and finishing pigs. Physicochemical measures of bulkiness determined on feed ingredients were DM, crude protein (CP), ether extract, ash, water holding capacity (WHC), bulk density, crude fibre (CF), neutral detergent fibre (NDF) and acid detergent fibre (ADF). Among the fibre sources, maize stover had the highest WHC, followed by veld grass, then lucerne hay, maize cob, sunflower husks, groundnut haulms, rice bran and saw dust. The greatest bulk densities (1.4 g DM/ml) were observed in lucerne hay and saw dust, whilst sunflower husk had the lowest (0.7 g DM/ml) (P < 0.001). Rice bran, maize cob and groundnut haulms were the most fermentable fibrous ingredients (P < 0.05). Based on differences in physicochemical properties, six fibres namely lucerne hay, maize cob, maize stover, veld grass, saw dust and sunflower husk were selected and used in formulating fibrous diets fed to growing pigs based on wideness in ranges of their bulk properties. Thirty-one complete diets were formulated by dilution of a conventional weaner feed with increment levels of each fibre source at 0, 80, 160, 240, 320 and 400 g/kg. Each of the diets was offered ad libitum to four of 124 pigs weighing 18.1 (s.d. 1.37) kg body weight, in individual pens, for four weeks. There was a linear decrease in scaled feed intake (SFI) (P < 0.001) as bulk density of the feeds increased. There was a quadratic relationship between SFI and WHC, NDF and ADF, respectively, whereby SFI increased up to a point when it reached its maximum and then started to decrease as bulkiness increased (P < 0.001). By use of the broken stick model, the maximum SFI marking the gut capacity of pigs was attained when WHC = 4.5 ± 1.25 g water/g DM (P< 0.001), NDF = 367 ± 29 g/kg DM (P < 0.001) and ADF = 138 ± 77 g/kg DM (P < 0.01), respectively. The SFI decreased linearly with an increase in bulk density of the feeds (P < 0.001). Four of 84 finishing pigs in individual pens, at 65 (s.d. 1.37) kg body weight were given, ad libitum to each of 21 diets containing graded levels of lucerne, maize cobs, saw dust and sunflower husk. There was a linear decrease in SFI (P < 0.001) as WHC increased. There was a quadratic decrease in SFI as CF (P < 0.001) and NDF (P < 0.01) increased. As CP increased, there was a quadratic increase in SFI (P < 0.01). In weaner pigs, an increase in WHC, NDF, ADF and bulk density constrains feed intake, thereby providing relationships that can be used to predict gut capacity. Conversely, measurements of feed bulk cannot provide relationships with intake that can be used to predict gut capacity in finishing pigs. / Thesis (M.Sc.Agric.)-University of KwaZulu-Natal, Pietermaritzburg.

Physicochemical properties of fibre-based diets were used to determine the influence of feed bulk on physicochemical properties of digesta within each segment of the gastrointestinal tract and digesta in pigs. In the first experiment, three pigs (14 ± 1.2 kg body weight (BW) were allocated to each of six diets containing maize cob levels at 0, 80, 160, 240, 320 and 400 g/kg DM inclusion levels for four weeks. All pigs were fed ad libitum. They were slaughtered, eviscerated and weights of the gut compartments were recorded, then contents of digesta from each segment were sampled for the determination of water concentration, water holding capacity (WHC) and swelling capacity (SWC). The WHC of digesta in the stomach, ileum and caecum decreased (P < 0.05) with maize cob inclusion level. The SWC in the stomach decreased with the inclusion level of maize cob meal. The SWC of caecal digesta increased with maize cob inclusion (P < 0.05). Physicochemical properties of digesta increased (P < 0.05) from the stomach to ileum then decreased as the digesta moved through the hindgut. In the second experiment, four fibres namely maize cob, lucerne hay, sunflower husk and citrus pulp were used. These fibres were used in formulating diets for finishing pigs. Twenty-one complete diets were formulated by dilution of a conventional feed with increment levels of each fibre source at 0, 80, 160, 240, 320 and 400 g/kg. Each of the diets was offered ad libitum to four of 84 pigs weighing 80.8 ± 8.15 kg body weight, in individual pens, for 30 days. Stomach weights increased linearly with an increase in neutral detergent fibre (NDF) but increased with quadratic functions with an increase in SWC of the diet (P <0.05). An increase in WHC of the fibrous diets increased linearly the WHC of the proximal colon (P <0.01) at a faster rate compared to the WHC of the distal colon (P <0.001). As the SWC of the diets increased, linear increases in SWC of the digesta in the stomach (P <0.01) and caecum (P <0.001) were observed. The WHC of the digesta was negatively correlated to SWC (P < 0.001) in the stomach. Scaled feed intake (SFI) decreased linearly with an increase in SWC of the diet (P < 0.001). There was no relationship between WHC of the diet and SFI (P > 0.05). There was a linear decrease (P < 0.01) in SFI of finishing pigs as the SWC of the digesta. It can be concluded that the swelling capacity of the diets and stomach digesta in stomach are accurate predictors of scaled feed intake. Swelling capacity had great influence in the stomach weights whilst other bulking properties, such as WHC and neutral detergent fibre, affected the weight and digesta properties in the caecum, proximal and distal colon. / M.Sc.Agric. University of KwaZulu-Natal, Pietermaritzburg 2013.

Degradation kinetics of carbohydrate fraction of commercial concentrate feeds for weaned calves, heifers, lactating and dry dairy cattle / Variations in composition and disappearance of nutrients in dairy cattle feeds are dictated by ingredients, methods of processing, storage while milk production levels depend on the animal, environmental factors and largely on pools of available carbohydrates, proteins, vitamins and minerals in the concentrate feeds. There is a wide variety of concentrates for dairy cattle on the formal and informal markets and dairy farmers need to be astute in selecting feeds appropriate for specific production periods and animals to sustain their businesses. Composition of nutrients displayed on concentrate containers is however inadequate for in-depth assessment of products. This study determined nutrient composition, rumen dry matter disappearance and microbial colonization on residual substrate on commercial concentrate feeds and simulated total mixed rations for dairy calves, heifers, lactating and dry cows based on common feeding guidelines. Equivalent feeds for each herd group were obtained from three suppliers in the formal markets in Gauteng province of South Africa, making a total of twelve. An analysis of the data on container labels for the herd groups displayed similar feed values, as also reflected on the recommendation Tables of Act 36: Feeds and Fertilizer bill 1947 of South Africa. / Agriculture, Animal Health and Human Ecology / M.Sc. (Agriculture)

Protein has been identified as a major reason that people purchase a horse feed, with anecdotal explanations offered for the poor prediction of response of horses to their rations, particularly in the sport horse market. The current research identifies through hierarchical cluster analysis that the myriad of riding feeds offered on the South African market fall into only four simple categories on the basis of wet chemistry. Feeds were subjected to the in vitro gas production technique (IVGPT) described by Pell and Schofield (1993), using equine faecal inoculum. Gas profiles, corrected for control fermentation profiles in the absence of substrate, were fitted to the model described by Campos et al. (2004) to derive GP kinetics. Gas production kinetics, and information in respect of pH, degradation efficiency, lag time and apparent and true digestibilities were obtained. The feeds were tested for glycaemic response in miniature horses using the hexokinase method with deproteinization using an auto analyser (Roche Diagnostics). Blood glucose parameters of feeds (mean, peak, slope and time to peak and area under the curve) in each group were compared by analysis of variance and regression with covariates. In vivo analysis of rates of passage and digestibility using using post-prandial percentages of acid insoluble marker collection was used to study the gastrointestinal process, to indicate foregut and hindgut compartmental flow. The need to balance nitrogen levels with a proportional supply of fermentable carbohydrate contradicts widely used protein intakes in the horse. In vitro fermentation was used in an analysis of nutrient synchrony, to identify optimal fermentative capacity for utilization of horse feeds. The characteristics of horse feeds were related to requirement and were composited in an analysis of the representation of horse feeds that would best reflect optimal utilization in the horse, to produce a method of feed characterisation that would lead to the optimal prediction of response of horses to feeds offered to South African horses. / Thesis (Ph.D.Agric.)-University of KwaZulu-Natal, Pietermaritzburg, 2011.

Three experiments were designed to find the most efficient and cost-effective method of meeting the changing amino acid requirements of male and female pigs during the growing period, using the minimum number of feeds. Trial one involved 144, and trial two 192 commercial crossbred (Landrace x Large White) pigs, eight to a pen, with sexes separate. Trial three involved 24 commercial crossbred (Landrace x Large White) pigs and 24 Dalland pigs each penned separately. The amino acid requirements (g/d) for the pigs in all three trials were determined for male and female Landrace x Large White pigs, respectively, using the Pig Growth Model (EFO Software Natal, 1995). Parameter values used for males and females were: growth rate (B, Id) 0.0107 and 0.0120; protein at maturity (Pmat, kg) 39.0 and 28.0; and lipid at maturity (Lpmat, kg) 2.60 and 3.89 respectively. In trials 1 and 2 two iso-energetic (DE 13.8 MJ/kg) basal feeds were formulated; Basal A being a high crude protein (CP) (347g CP/kg) feed designed to meet the amino acid requirements of a male at 20 kg liveweight, while Basal B was a low protein feed (l34g CP/kg) designed to meet the amino acid requirements of a female at 88 kg, thus providing for the most- and the least-demanding pigs on the trial. In the third trial, two iso-energetic (DE 13.8 MJ/kg) basal feeds were again formulated; Basal A being a high crude protein (CP) (347g CP/kg) feed designed to be 20% higher than the amino acid requirements of a male at 20 kg liveweight, while Basal B was a low protein feed (l34g CP/kg) designed to be 20% lower than the amino acid requirements of a female at 88 kg, once again providing for the most- and the least-demanding pigs on the trial. Trials 1 and 2 began when the median weight of pigs in each pen reached 20kg, and were terminated at a pen median of 85 kg liveweight. In the third trial each pig was started on trial when it reached 20kg and was terminated at a weight of 85kg. The first trial involved a phase feeding schedule (20-40, 40-60 and 60-85kg liveweight). The two basal feeds were blended in different proportions to create three feeds per phase: lysine contents in each of the phases in Treatment 1 (TI) were: 11, 8.68 and 7.26g/kg; in T2 they were 9.93, 7.58 and 6.24g/kg; and in T3, 8.85, 6.48 and 5.22g/kg. From the analysis it was established that ADO and time to reach 85kg were the only variables to show significance. ADO exhibited a significant sex x treatment interaction. There were significant differences between treatments for time taken to reach slaughter weight. In addition to a treatment effect there was a sex x treatment interaction for time to slaughter weight. It was expected that males on Tl and females on T3 would exhibit the most efficient performance for their respective sex since these treatments were specifically formulated to meet their requirements. Midway through the trial the pigs contracted enteritis, this affecting the outcome of the trial by inhibiting the potential growth of the pigs. The results of the trial indicated that dietary protein level affected the time taken to reach slaughter weight. This led to the second trial where four treatments were applied. Three of the four treatments followed a fixed feeding schedule, making use of the two basal feeds and a 1: 1 blend of these. The fourth treatment followed a phase feeding schedule, differing between the males (20-65, 65-75 and 75-85 kg liveweight) and the females (20-35, 35-75 and 75-85 kg liveweight). This treatment also made use of the two basal feeds and a 1: 1 blend of these. From the analysis it was found that there were no significant effects of sex and no interactions between feeding treatments and sex; however, ADO, FI, FCE, back fat thickness, time taken to reach 85kg and cost/kg gain were all significantly affected by the feeding treatments. Carcass lean, carcass lipid and total body lipid were also significantly affected by the feeding treatment. This trial was conducted to determine the extent to which differences in growth rate, food intake and carcass lipid could be altered by dietary means. The effect of the level of feed protein was once again shown to be of importance when feeding growing pigs. The third trial was designed to test the efficiency with which two strains make use of the dietary protein supplied. Three treatments were applied: Tl was a choice-feeding treatment in which the pigs were offered the two basal feeds simultaneously. T2 and T3 followed a phase feeding schedule (20-40, 40-60 and 60-85kg liveweight). The two basal feeds were blended in various proportions to create three feeds per phase: the lysine contents in each of the phases in T2 were: 12.2, 10.0 and 7.26g/kg; and in T3 8.1, 6.7 and 5.1g/kg. There were significant sex effects as well as strain x feeding treatment interactions. All variables, ADG, FI, FCE, time taken to reach 85kg and cost/kg gain, with the exception of back fat thickness, showed significance. It was expected that the Dalland strain would perform better than the Cross strain; however, this was not the case, indicating the need for further research into the possibility of feeding according to the genetic makeup of the animal. The importance of meeting the amino acid requirements of the growing pig was evident when summarising the results of the three trials reported here. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.