Porous asphalt pavements offer an alternative technology for stormwater management. A porous asphalt pavement differs from traditional asphalt pavement designs in that the structure permits fluids to pass freely through it, reducing or controlling the amount of run-off from the surrounding area. By allowing precipitation and run-off to flow through the structure, this pavement type functions as an additional stormwater management technique. The overall benefits of porous asphalt pavements may include both environmental and safety benefits including improved stormwater management, improved skid resistance, reduction of spray to drivers and pedestrians, as well as a potential for noise reduction. With increasing environmental awareness and an evolving paradigm shift in stormwater management techniques, this research aims to provide guidance for Canadian engineers, contractors, and government agencies on the design of porous asphalt pavement structures. One of the keys to the success of this pavement type is in the design of the asphalt mix. The air void percentage, which is ultimately related to the effectiveness of the pavement to adequately control the runoff, is a critical component of the mix. However, special consideration is required in order to obtain higher air void percentages while maintaining strength and durability within a cold climate. The objectives of this study were to evaluate several laboratory porous asphalt mix designs for durability and strength in cold climate conditions. The porous asphalt mixes consisted of a porous asphalt Superpave mix design method whereby the asphalt binder type was varied. Performance testing of the porous asphalt including draindown susceptibility, moisture-induced damage susceptibility, dynamic modulus, and permeability testing were completed. Based on the preliminary laboratory results, an optimal porous asphalt mix was recommended for use in a Canadian climate. Initial design guidelines for porous asphalt were provided based on preliminary findings and hydrological analysis.

Porous asphalt pavements offer an alternative technology for stormwater management. A porous asphalt pavement differs from traditional asphalt pavement designs in that the structure permits fluids to pass freely through it, reducing or controlling the amount of run-off from the surrounding area. By allowing precipitation and run-off to flow through the structure, this pavement type functions as an additional stormwater management technique. The overall benefits of porous asphalt pavements may include both environmental and safety benefits including improved stormwater management, improved skid resistance, reduction of spray to drivers and pedestrians, as well as a potential for noise reduction. With increasing environmental awareness and an evolving paradigm shift in stormwater management techniques, this research aims to provide guidance for Canadian engineers, contractors, and government agencies on the design of porous asphalt pavement structures. One of the keys to the success of this pavement type is in the design of the asphalt mix. The air void percentage, which is ultimately related to the effectiveness of the pavement to adequately control the runoff, is a critical component of the mix. However, special consideration is required in order to obtain higher air void percentages while maintaining strength and durability within a cold climate. The objectives of this study were to evaluate several laboratory porous asphalt mix designs for durability and strength in cold climate conditions. The porous asphalt mixes consisted of a porous asphalt Superpave mix design method whereby the asphalt binder type was varied. Performance testing of the porous asphalt including draindown susceptibility, moisture-induced damage susceptibility, dynamic modulus, and permeability testing were completed. Based on the preliminary laboratory results, an optimal porous asphalt mix was recommended for use in a Canadian climate. Initial design guidelines for porous asphalt were provided based on preliminary findings and hydrological analysis.

Pervious concrete pavement has the capacity to perform as two types of infrastructure: a pavement; and a stormwater management solution. It is a low impact development as it does not alter the natural hydrological cycle when implemented, unlike a conventional impermeable pavement. This research represents some of the initial investigations into pervious concrete pavement in Canada. The two research hypotheses of this research were the following: 1. Pervious concrete pavement can be successfully planned, designed, constructed and maintained in Canada for successful performance based on surface evaluations of permeability rate and surface condition. 2. Verification that the subsurface drainage capabilities of pervious concrete pavement are as described in literature and can be quantified using instrumentation. Through monitoring of the design, construction, performance and maintenance of five field sites across Canada and various laboratory pavement slabs, the behaviour of pervious concrete pavement in freeze-thaw conditions has been evaluated. This thesis presents the findings from the various phases of the life cycle of pervious concrete pavement: planning; design; construction; and maintenance. An interpretation of the performance of pervious concrete pavement both from the perspective of the surface and subsurface is included. The various field sites led to pervious concrete being used in areas exposed to static or parked traffic and areas with slow moving traffic. At the two sites that included static and slow moving traffic, the permeability performance was better in the areas of static traffic than those with moving traffic. Each of the field sites had a unique mix design and some had multiple variations of one basic mix design. The relationship between the void content and hardened density of the pervious concrete cores was linear with none of the cores being visually identified as outliers. Substantial deterioration in pavement structure performance was identified at one site. Other field sites showed changes in structural capacity over the monitoring timeline. However, no locations of substantial decreases in structural capacity were identified. The surface condition of the sites over the analysis period indicated that compaction to the surface during construction was helpful in constructing a quality pavement. The results of the project indicated that pervious concrete will crack when joints are not included and may also crack similarly to conventional impermeable concrete pavements if joints are spaced too widely or do not match joints of adjacent pavement. Washing the pervious concrete pavement surface with a large hose or garden hose was found to be the most effective in improving permeability across a site and also in increasing the permeability of the pervious concrete. The initial permeability of the pervious concrete pavement was found to influence future performance. Freeze-thaw cycling and moisture were found to alter the internal structure of pervious concrete. However, did not generally lead to surface distress development. The application of sand as a winter maintenance method decreased the permeability, as did the use of a salt solution. However, neither winter maintenance method led to the permeability rates of laboratory slabs dropping below an acceptable level. All three slabs loaded with a salt solution deteriorated to a point where the slabs had failed. The initial permeability of the field sites proved to be important and although some sites started with what appeared to be very high permeability rates, these sites were successful in the multiple year evaluation in maintaining adequate permeability rates. The types of surface distresses that developed in the cores and slabs in the laboratory were generally not substantially worse at the field sites, suggesting that pedestrian and vehicle traffic do not necessarily escalate distresses caused by the Canadian climate and corresponding winter activities. The subsurface drainage that was quantified by the instrumentation included in three field sites confirmed observations from the surface of the pavement and exceeded other expectations. Two field sites exhibited limited drainage capabilities on the surface of the pervious concrete pavement, one shortly after construction, and the other within a year following construction. The subsurface analysis quantified and confirmed that moisture was not able to drain completely vertically through the pavement structures at these two sites due to the limited access in the pervious concrete pavement surface. In comparison, the subsurface drainage at another site surpassed the assumed behaviour of pervious concrete pavement structures. The pavement structure in general at this site was highly permeable and this was identified as moisture was not observed to be collecting in the bottom of the storage base layer at any time or for any period of time. The successful overall drainage performance of this site demonstrates the ability to effectively use pervious concrete pavement in Canada.

A gel is a cross-linked polymer network that spans an entire liquid medium; its properties depend strongly on the interaction of the polymer and the liquid medium. There are various ways to induce gelation in different systems such as altering temperature or pH. In this study, phenol extracted protein fractions from non-acclimated (NA) and cold-acclimated (CA) winter rye (Secale cereale L. cv Musketeer) leaf tissue were subjected to freeze-thaw treatment. Gelation was induced in the NA and CA extracts after repeated freeze-thaw treatments, accompanied by a change in sample rheological properties. Further experimentation revealed that gel formation only occurred at high pH (pH 12.0) and that a minimum of 3 to 4 freeze-thaw cycles were required. The viscosity of the protein gel increased 5.7- to 9.5-fold in the NA and CA extracts respectively upon freeze-thaw. Experiments optimizing the extraction conditions and protein concentration were also performed. The gel was stable and only a specific combination of chaotropic agent, anionic surfactant and reducing agent such as urea, sodium docecyl sulfate (SDS) and â-mercaptoethanol (â-ME) with heating could disrupt the gel network. The gel was composed of several proteins in the extracts as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Based on SDS-PAGE analysis, ribulose-1,5-bisphosphate carboxylase oxygenase (Rubisco) was identified as the major protein component in the gel. Various experiments were performed to assess the role of Rubisco in gel formation; however, the results were inconclusive. It is suggested that these extracts may contain antifreeze proteins (AFPs) that have been demonstrated to form amyloid gels upon freeze-thaw. Further studies examining the composition and mechanism of gel formation may result in a future role for this material in the food industry.

Nisin is a naturally occurring food antimicrobial that inhibits many Grampositive pathogens, including Listeria monocytogenes, a bacterial pathogen responsible for ~500 deaths in the U.S. annually. Factors known to counteract the nisin activity in a food matrix include: antimicrobial interaction with food components, insolubility, protease inactivation, and target cell-driven envelope modifications. Encapsulating nisin in liposomes can help protect nisin functionality by regulating its introduction to the external environment. The objectives of this study were to determine the encapsulation efficiency (%EE) of nisin within liposomes as a function of encapsulation method and the capacity of liposomal nisin to inhibit L. monocytogenes in fluid milk. Phosphatidylcholine (PC) and phosphotidyl-DL-glycerol (PG) were used to prepare three lipid molar formulations: PC, PC/PG 7:3, and PC/PG 6:4 (mol.%). Liposomes were formulated to entrap the self-quenching fluorophore calcein and nisin. Unencapsulated analyte was removed via size-exclusion chromatography, and percent EE was determined. To determine antilisterial activity of liposomes, fluid milk samples containing L. monocytogenes (4 log10 CFU/mL) in combination with liposomal or unencapsulated nisin at 50 IU/mL were mixed and aerobically stored at 5 degrees C and 20 degrees C. Surviving L. monocytogenes were enumerated via plating on a non-selective microbiological medium after 0, 1, 3, 6, 12, 24, 48, and 72 hours of incubation. Encapsulation of nisin via extrusion resulted in a mean EE% of 84.20%, 77.33% and 80.78% for PC, PC/PG 7:3, and PC/PG 6:4 liposomes, respectively. Freeze-thaw cycling formed liposomes without detectable fluorophore entrapment. L. monocytogenes populations grew to 5 log10 CFU/mL after 72 hours at 5 degrees C and 8 log10 CFU/mL at 20 degrees C after 48 hours. Unencapsulated nisin exerted statistically greater inhibition of Listeria in skim milk compared to liposomal nisin, regardless of incubation temperature. No statistically significant differences in Listeria populations exposed to free or encapsulated nisin in whole milk were observed at either incubation temperature. Results indicate storage temperature and presence of milk fat exert greater influence then nisin delivery (free vs. encapsulated) over Listeria inhibition. Further research is needed to confirm these findings and develop more effective means of liposome entrapment of nisin for the inhibition of foodborne bacterial pathogens.

Concrete marine structures are typically exposed to harsh marine environments where the ingress of chloride ions can lead to corrosion of steel reinforcing bars, reducing both strength and service life; therefore, concrete must be proportioned to resist these environments. Current recommendations for concrete mixtures and plastic shrinkage cracking both reduce the resistance to chloride ingress. The main objective of this thesis was to understand the benefits of fiber addition to concrete exposed to chlorides and quantify those benefits, which would lead to a concrete mixture suitable for marine structures in freeze thaw environments. The research program tested two different fibers in a total of nine concrete mixtures. The results demonstrated that fiber addition at dosages up to 0.33 % by volume resulted in significant reduction or elimination of plastic shrinkage cracking and the chloride tests determined that the ternary FRC mixtures had the best resistance to chloride diffusion.

Mercury (Hg) is a potent human toxin and a persistent global pollutant with unique properties and environmental behaviours which make it difficult to model and understand. While anthropogenic mercury sources are well understood along with the impacts on ecosystems and human populations, the processes and transformations which govern environmental cycling lack the same level of understanding. Concentrations in Arctic environments are a specific concern, along with cycling behaviours in regions spanning from temperate to Arctic climates. The objective of this experiment was the investigation and characterization of the mechanisms which promote elemental mercury (Hg^0) flux from soils in these environments during simulated seasonal cycling. A laboratory scale experiment was conducted which used a Dynamic Flux Chamber (DFC) to monitor Hg^0 flux from a naturally Hg enriched soil during temperature cycling relevant to cold environments. The results, which were split into freeze-thaw (FT) and sub-zero (SZ) cycles, showed that Hg^0 flux from frozen soils remains active during temperature cycling. During FT cycles, Hg^0 flux is controlled by soil temperature and energy entering the system, with a linear increase in flux for increases in energy. This response is produced from the entire soil column. During SZ cycles, Hg^0 flux is produced only in the thin soil surface layer and is controlled by the air temperature at the soil-air interface. A decrease in the DFC air temperature was observed to produce an increase in flux, with an inverse relationship controlled by a separate mechanism than the FT cycle response. Recommendations for modifications to the experimental set-up and methodology have been made to improve the accuracy of the results and confirm the behaviours characterized during this study. / Natural Sciences and Engineering Research Council of Canada (NSERC)

An experimental waste-rock pile (50m x 60m x 15m, 0.053 wt. % S) was constructed at the Diavik Diamond Mine, in the Northwest Territories, Canada to evaluate the generation of acid-rock drainage and the seasonal and annual release of various metals in drainage leachate. A dense internal instrumentation network enabled well resolved observations of temperature, air content, water content, fluid flow, microbiology, mineralogy and geochemistry within the waste-rock pile. Water samples were collected from soil water solution samplers (SWSSs) to measure core pore-water characteristics, from 4 m2 to 16 m2 scale basal collection lysimeters (BCLs) to measure core leachate characteristics, and from basal drains (3000 m2 basal area) to measure aggregated leachate characteristics. Monitoring of pore-water geochemistry within the core of the test pile indicated an evolving weathering front characterized by changes in predominant acid-consuming mineral-dissolution reactions. Initially, acid neutralization occurred through dissolution of carbonate minerals. A subsequent decline in pH was limited by acid neutralization through dissolution of Al- and Fe-bearing minerals. This lower pH environment was accompanied by increasing concentrations of SO4, Al, Fe, Ni, Co, Cu, Zn, Cd, Ca, Mg, K, Na and Si. Annual drainage cycles in the core of the test pile were characterized by distinct, high concentration ‘spring flushes’ followed by a steady decline of all dissolved constituents with minimums prior to freeze-up. Core trends were typical of freshets observed in polar environments and primarily explained by a combination of fluid residence time and the build-up of oxidation products over the winter. The opposite trend was observed in the aggregated pile drainage, whereby early-season low-concentration leachate was derived from snowmelt and batter flow and late-season high-concentration leachate was dominated by contributions from the core of the pile. Thermal data demonstrating the annual freeze-thaw cycle was used to delineate core and batter subsystems within the pile. Mean annual solute concentrations and geochemical speciation modelling results revealed two different environments within these subsystems. Concentrations in the core of the test pile were 2.5 to 8.5 times greater than concentrations in the batter. Dilution through snowmelt contributions and shorter flow pathways were expected to control solute concentrations in the batter subsystem. Aggregated basal leachate loading exhibited a linear annual increase for all ARD reaction products, with the maximum annual release observed in 2012. Between 2007 and 2012 core flow accounted for 13 % of the total drainage volume; whereas 35 to 51 % of major and trace metal loads were attributed to this zone. Parameter correlation analysis and core contribution estimates were used to identify common source minerals for elements and infer mechanisms controlling the mobility of dissolved metals (sorption, co-precipitation and precipitation-dissolution). By 2012, the release of pyrrhotite oxidation products from the < 5 mm reactive fraction of waste rock reached as high as 5.1 %, 9.0 %, 7.2 % and < 0.1 % for S, Ni, Co and Fe respectively. The results of this study indicate that a comprehensive understanding of thermal cycling is imperative when estimating seasonal and annual releases of weathering products from waste rock. Observations of active zone dynamics and the temporal and spatial evolution of waste rock drainage loading recognized in this study will assist in the advancement of reactive transport models describing ARD in cold climates.

Knowing the control of landscape position in greenhouse gas (GHG) emission from the Prairie pothole region is necessary to provide reliable emission estimates needed to formulate strategies for reducing emission from the region. Presented here are results of a study investigating the control of landscape position on the flux of nitrous oxide (N2O) and methane (CH4) from an agricultural soil. Field flux of N2O and CH4 and associated soil parameters from the Upper, Middle, Lower and Riparian slope positions were monitored from spring to fall of 2005, and spring of 2006, at the Manitoba Zero-Tillage Research Association (MTRZA) farm, 17.6km North of Brandon, MB. The field site consisted of a transect of 128 chambers segmented into the four landscape positions, with either all chambers or a subset of the chambers (32) sampled on select days. Spring thaw is an important period for annual inventory of N2O emission, thus, soil samples were also collected from the four slope positions in fall 2005, and treated in the laboratory to examine how antecedent moisture and landscape position affect the freeze-thaw emission of N2O from soil. Daily emissions of N2O and CH4 for 2005 were generally higher than for 2006, the former being a wetter year. There was high temporal variability in N2O and CH4 emission, with high fluxes associated with events like spring thaw and fertilizer application in the case of N2O, and rapid changes in soil moisture and temperature in the case of CH4. There was a high occurrence of hotspots for N2O emission at the Lower slope, associated with its high soil water-filled porosity (WFP) and carbon (C) availability. The Riparian zone was not a source of N2O emission, despite its soil WFP and organic C being comparable with the Lower slope. The hotspot for CH4 emission was located at the Riparian zone, associated with its high soil WFP and C availability. The Upper and Middle slope positions gave low emission or consumed CH4, associated with having low soil WFP and available C. This pattern in N2O and CH4 emission over the landscape was consistent with examination of entire 128 chambers on the transect or the 32 subset chambers. Significantly lowering the antecedent moisture content of soil by drying eliminated the freeze-thaw emission of N2O, despite the addition of nitrate to the soil. This was linked to drying slightly reducing the denitrifying enzyme activity (DEA) of soil. The highest and earliest freeze-thaw emission of N2O was from the Riparian zone, associated with its high antecedent moisture content, DEA and total organic C content. The addition of nitrate to soil before freezing failed to enhance freeze-thaw emission of N2O from the Upper, Middle and Lower slope positions, but increased emission three-fold for the Riparian zone. Despite the greater potential of the Riparian zone to produce N2O at thaw compared to the Upland slopes, there was no spring-thaw emission of N2O from the zone on the field. This was because this zone did not freeze over the winter, due to insulation by high and persistent snow cover, vegetation and saturated condition. The denitrifying potential and freeze-thaw N2O emission increased in going from the Upper to the Lower slope position, similar to the pattern of N2O emission observed on the field. The localization of hotspots for N2O and CH4 emission within the landscape was therefore found to be driven by soil moisture and C availability. When estimating GHG emission from soil, higher emission index for N2O and CH4 should be given to poorly-drained cropped and vegetated areas of the landscape, respectively. The high potential of the Riparian zone for spring-thaw emission of N2O should not be discountenanced when conducting annual inventory of N2O emission at the landscape scale. When fall soil moisture is high, snow cover is low, and winter temperature is very cold, freeze-thaw emission of N2O at the Riparian zones of the Prairie pothole region may be very high.

Pervious concrete pavement has the capacity to perform as two types of infrastructure: a pavement; and a stormwater management solution. It is a low impact development as it does not alter the natural hydrological cycle when implemented, unlike a conventional impermeable pavement. This research represents some of the initial investigations into pervious concrete pavement in Canada. The two research hypotheses of this research were the following: 1. Pervious concrete pavement can be successfully planned, designed, constructed and maintained in Canada for successful performance based on surface evaluations of permeability rate and surface condition. 2. Verification that the subsurface drainage capabilities of pervious concrete pavement are as described in literature and can be quantified using instrumentation. Through monitoring of the design, construction, performance and maintenance of five field sites across Canada and various laboratory pavement slabs, the behaviour of pervious concrete pavement in freeze-thaw conditions has been evaluated. This thesis presents the findings from the various phases of the life cycle of pervious concrete pavement: planning; design; construction; and maintenance. An interpretation of the performance of pervious concrete pavement both from the perspective of the surface and subsurface is included. The various field sites led to pervious concrete being used in areas exposed to static or parked traffic and areas with slow moving traffic. At the two sites that included static and slow moving traffic, the permeability performance was better in the areas of static traffic than those with moving traffic. Each of the field sites had a unique mix design and some had multiple variations of one basic mix design. The relationship between the void content and hardened density of the pervious concrete cores was linear with none of the cores being visually identified as outliers. Substantial deterioration in pavement structure performance was identified at one site. Other field sites showed changes in structural capacity over the monitoring timeline. However, no locations of substantial decreases in structural capacity were identified. The surface condition of the sites over the analysis period indicated that compaction to the surface during construction was helpful in constructing a quality pavement. The results of the project indicated that pervious concrete will crack when joints are not included and may also crack similarly to conventional impermeable concrete pavements if joints are spaced too widely or do not match joints of adjacent pavement. Washing the pervious concrete pavement surface with a large hose or garden hose was found to be the most effective in improving permeability across a site and also in increasing the permeability of the pervious concrete. The initial permeability of the pervious concrete pavement was found to influence future performance. Freeze-thaw cycling and moisture were found to alter the internal structure of pervious concrete. However, did not generally lead to surface distress development. The application of sand as a winter maintenance method decreased the permeability, as did the use of a salt solution. However, neither winter maintenance method led to the permeability rates of laboratory slabs dropping below an acceptable level. All three slabs loaded with a salt solution deteriorated to a point where the slabs had failed. The initial permeability of the field sites proved to be important and although some sites started with what appeared to be very high permeability rates, these sites were successful in the multiple year evaluation in maintaining adequate permeability rates. The types of surface distresses that developed in the cores and slabs in the laboratory were generally not substantially worse at the field sites, suggesting that pedestrian and vehicle traffic do not necessarily escalate distresses caused by the Canadian climate and corresponding winter activities. The subsurface drainage that was quantified by the instrumentation included in three field sites confirmed observations from the surface of the pavement and exceeded other expectations. Two field sites exhibited limited drainage capabilities on the surface of the pervious concrete pavement, one shortly after construction, and the other within a year following construction. The subsurface analysis quantified and confirmed that moisture was not able to drain completely vertically through the pavement structures at these two sites due to the limited access in the pervious concrete pavement surface. In comparison, the subsurface drainage at another site surpassed the assumed behaviour of pervious concrete pavement structures. The pavement structure in general at this site was highly permeable and this was identified as moisture was not observed to be collecting in the bottom of the storage base layer at any time or for any period of time. The successful overall drainage performance of this site demonstrates the ability to effectively use pervious concrete pavement in Canada.