Th.--Philos.--Paris 1, 1986.

Th.--Psychol.--Paris 7, 1986.

Diss.--Philosophische Fakultät I--Zürich--Universität 1994.

No description available.

No description available.

The serotonin-1A (5-HT1A) receptor plays an important role in the regulation of the serotonin (5-HT) system as an autoreceptor on 5-HT neurons. The transcription factor CC2D1A/Freud-1 is a potent repressor of the 5-HT1A promoter in neuronal but not in non-neuronal cells. The clinical relevance of Freud-1 is evident in a naturally occurring mutation, resulting in a truncated form of Freud-1 lacking its C-terminal half, that is associated with non syndromic mental retardation in humans. Thus, it is of interest to clarify the structure and function of Freud-1. As Freud-1 was shown to interact with the transcriptional regulator Brg1 at the 5-HT1A promoter, identification of the structural domains mediating the Brg1/Freud-1 interaction is required to assess the role of Brg1 in Freud-1 repression. In this study, I used pull-down assays with recombinant proteins, co-immunoprecipitation studies and immunofluorescent staining with confocal microscopy to show that Freud-1 interacts directly with the C-terminus of Brg1 and that the C-terminal domain of Freud-1 is required for this interaction.

Mémoire numérisé par la Direction des bibliothèques de l'Université de Montréal.

The thesis seeks to explore one of the most singular features of Freudian thought, his radical position in the history of the ideas about language. One of my chief claims is that the Freudian endeavour is not oriented towards a conclusive theory of language as such, but of the conditions of its destruction, its exhaustion, its silence. The obscure centre of Freud's work, the passion for the shattering of language, manifests itself both as an affirmation and as a dissipation of the sense of speech, which cast some light upon the cardinal role of the notion of secrecy, not only in his comprension of language, but also in his conception of subjectivity. Thus, secrecy can be conceived as a fundamental feature of different facets of his writings. The first facet exhibits psychoanalysis as the inheritor of the progressive emergence of silence in the core of modern thought. I argue that the logic of secrecy which appears in Freud's early writings enacts the historical emergence of secrecy which pervaded different discourses of the nineteenth century. This singular logic had its origin at the confluence of the exalted discourses which enthrowned observation and experience in the positivistic conception of knowledge bred by the Enlightenment, the obscure cults of magnetism and the speculative conceptions of subjectivity which emerged from the crisis of the Enlightenment, with the rising of Romanticism and its powerful effects on the Western culture. The second facet exhibits the logic of secrecy as expressed by the acts of language. Secrecy introduces an inner discord in the meaning of signs: it reveals the obsolescence of the referential notion of truth. Allegory emerges from this discord as a privileged aesthetic and theoretical expression. Freud's theoretical creativity canceled the significance of the referential, discursive notion of truth with the violent implications of the notion of primary thought processes and a conception of primal experiences of pleasure and pain irreducible to the narrow margins of rationality. The radical dissipation of the conventional foundations of semantic truth brought into focus an aesthetic -Baroque~ conception of subjectivity. This vision pervades Freud's notion of psychical processes, and engendered a constellation of forms of theoretical expression: psychical processes were apprehended by allegorical figures: the fold, rhythm, movement, displacement involving paradoxical temporalities which offered a contrasting landscape of thought processes that informed desire and aroused anxiety; Freud created thus a theoretical chiaroscuro. A third facet involves two further Freudian notions: sexuality and pain. One of them, sexuality, is almost too notorious in Freud's work; the other, pain, was permanently and explicitly displaced, silenced, excluded. or even emphatically avoided in Freud's writings, and yet it is an notion inherent in his conception of subjectivity. Freud's subversion of the modem notion of experience might be thought of as founded upon his conception of the experience of pain as a constitution~ dimension of subjectivity, as its unspeakable, unapproachable, secret centre.

Thesis (Ph.D.)--York University, 1997. Graduate Programme in Social and Political Thought. / Typescript. Includes bibliographical references (leaves 380-412). Also available on the Internet. MODE OF ACCESS via web browser by entering the following URL: http://wwwlib.umi.com/cr/yorku/fullcit?pNQ99163

The serotonin-1A (5-HT1A) receptor plays an important role in the regulation of the serotonin (5-HT) system as an autoreceptor on 5-HT neurons. The transcription factor CC2D1A/Freud-1 is a potent repressor of the 5-HT1A promoter in neuronal but not in non-neuronal cells. The clinical relevance of Freud-1 is evident in a naturally occurring mutation, resulting in a truncated form of Freud-1 lacking its C-terminal half, that is associated with non syndromic mental retardation in humans. Thus, it is of interest to clarify the structure and function of Freud-1. As Freud-1 was shown to interact with the transcriptional regulator Brg1 at the 5-HT1A promoter, identification of the structural domains mediating the Brg1/Freud-1 interaction is required to assess the role of Brg1 in Freud-1 repression. In this study, I used pull-down assays with recombinant proteins, co-immunoprecipitation studies and immunofluorescent staining with confocal microscopy to show that Freud-1 interacts directly with the C-terminus of Brg1 and that the C-terminal domain of Freud-1 is required for this interaction.