Made available in DSpace on 2014-06-11T19:23:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-08-23Bitstream added on 2014-06-13T20:50:46Z : No. of bitstreams: 1 verdugo_mr_me_botfmvz_parcial.pdf: 88322 bytes, checksum: f575d6903d8555b289175acf9f1cd578 (MD5) Bitstreams deleted on 2014-10-03T16:24:41Z: verdugo_mr_me_botfmvz_parcial.pdf,Bitstream added on 2014-10-03T16:27:38Z : No. of bitstreams: 2 verdugo_mr_me_botfmvz_parcial.pdf.txt: 17709 bytes, checksum: 25b75f5c7613356dd5f6236b39428ee4 (MD5) 000696081.pdf: 261540 bytes, checksum: c5e68e6635b2268cdef1b12beeec94d6 (MD5) Bitstreams deleted on 2014-10-03T16:33:15Z: 000696081.pdf,Bitstream added on 2014-10-03T16:43:29Z : No. of bitstreams: 2 verdugo_mr_me_botfmvz_parcial.pdf.txt: 17709 bytes, checksum: 25b75f5c7613356dd5f6236b39428ee4 (MD5) 000696081.pdf: 261540 bytes, checksum: c5e68e6635b2268cdef1b12beeec94d6 (MD5) Bitstreams deleted on 2014-10-03T16:48:55Z: 000696081.pdf,Bitstream added on 2014-10-03T16:49:47Z : No. of bitstreams: 1 000696081.pdf: 261540 bytes, checksum: c5e68e6635b2268cdef1b12beeec94d6 (MD5) Bitstreams deleted on 2014-10-27T11:47:10Z: 000696081.pdf,Bitstream added on 2014-10-27T11:48:05Z : No. of bitstreams: 1 000696081.pdf: 261540 bytes, checksum: c5e68e6635b2268cdef1b12beeec94d6 (MD5) / Universidade Estadual Paulista (UNESP) / O estudo teve por objetivo comparar parâmetros cinéticos e têmporo-espacias, em gatos machos e fêmeas clinicamente saudáveis, com o emprego de uma plataforma de pressão. Foram utilizados 18 gatos adultos, sem raça definida, divididos em dois grupos: Grupo 1 - 10 machos, com peso variando entre 3,1 e 6,8Kg; Grupo 2 - oito fêmeas, com peso variando entre 3,3 e 4,75Kg. Os parâmetros cinéticos e têmporo-espacias foram aferidos com emprego de uma plataforma de pressão de 1.951mm x 447 mm. Para cada animal foram selecionadas cinco trilhas válidas, que foram consideradas válidas se o gato caminhou na velocidade de 0,54 até 0,74 m/s e aceleração de -0,20 e 0,20 m/s2. Foram determinados o Pico de Força Vertical (PFV), o Impulso Vertical (IV), a duração do ciclo da passada completa, o tempo de apoio, o tempo de balanço, o comprimento da passada e a distribuição do peso para os quatro membros. Para cada grupo, não foram observadas diferenças entre os lados direito e esquerdo, tanto nos membros pélvicos como torácicos. Para ambos os grupos, o PFV, o IV e a distribuição de peso foram estatisticamente maiores para o membro torácico que o membro pélvico. Com exceção do comprimento da passada, que foi maior para os machos, as demais variáveis não apresentaram diferenças entre os grupos. Houve diferença no comprimento dos membros torácicos e pélvicos, sendo maior para os gatos machos. Houve correlação entre o comprimento da passada e o comprimento dos membros. Foi possível concluir que entre os gatos machos e fêmeas a única diferença observada foi no comprimento da passada, consequente ao maior tamanho corpóreo dos gatos machos. Essa diferença não foi suficiente para interferir nos valores cinéticos e demais parâmetros têmporo-espaciais / The aim of this study was to compare kinetic and temporospatial parameters in clinically healthy male and female cats with a pressure-sensing walkway. Eighteen crossbreed adult cats were divided into two groups: G1 – ten male cats weighing 3.1-6.8 kg; G2 – eight female cats weighing 3.3-4.75 kg. The data from the first five valid trials were collected for each cat. A trial was considered valid if the cat walked within the velocity of 0.54-0.74 m/s and acceleration from -0.20 to 0.20 m/s2. The peak vertical force (PVF), vertical impulse (VI), gait cycle time, stance time, swing time, stride length, and the percentage body weight distribution among the four limbs were determined. For each group, no significant differences were observed, in either the forelimbs or the hind limbs, between the left and right sides for any of the variables. For both groups PVF (%BW), VI, and percentage of body weight distribution were higher to forelimbs than hind limbs. Except the stride length that was larger for male, the other kinetic and temporospatial variables did not show any statistical significance between groups. There was difference in the lengths of the forelimbs and hind limbs that were larger in male cats. There was correlation between stride length and length of the limbs. In conclusion, the only difference observed between males and female cats was the stride length, and this was due to greater body size of male cats. The difference was not enough to interfere in the kinetic data and other temporospatial parameters

Made available in DSpace on 2016-05-17T16:51:08Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-05-15. Added 1 bitstream(s) on 2016-05-17T16:54:42Z : No. of bitstreams: 1 000863861.pdf: 1356360 bytes, checksum: 18b85330b53418de931a17e7f535d1fb (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Considering the complexity of the immune response against Leishmania spp. infection in different species and the lack of clinical signs in the majority of cats with visceral leishmaniasis, this study aimed to evaluate the gene expression of some cytokines involved in cellular (IFN-, TNF α, IL-2) and humoral (IL-5) immune response by real-time PCR, on spleen samples from 12 cats naturally infected by Leishmania spp., comparing to the immune response of four healthy cats; and to verify the correlation between the parasite load in the bone marrow and the gene expression of cytokines in the spleen of infected animals. An increase of 1.53 times in IFN- gene expression was observed in infected cats when compared to healthy cats (p=0.0005). Statistically significant differences were not observed in gene expression of the cytokines evaluated between infected and healthy cats (TNF-α p=0.4203, IL-2 p=0.1968, IL-5 p=0.6390). Correlation was not observed between the parasite load in the bone marrow and the gene expression of cytokines in the spleen of cats infected by Leishmania spp. (IFN- p = 0.1570; TNF- p = 0.5702; IL-2 p = 0.6081; IL-5 p = 0.2329). These results suggest that IFN- may play an important role in the protective mechanisms against feline leishmaniasis / FAPESP: 2013/02394-6 / FAPESP: 2012/10365-3