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Studies on Subterranean clover mottle virus towards development of a gene silencing vector /Fosu-Nyarko, John. January 2005 (has links)
Thesis (Ph.D.)--Murdoch University, 2005. / Thesis submitted to the Division of Science and Engineering. Bibliography: leaves 184-207.
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Gene organization of the lobster (Homarus americanus) Gonad inhibiting hormone, and its functional analysis in relation to vitellogenesis by RNA interferenceSo, King-yip, Ken. January 2008 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2008. / Includes bibliographical references (leaf 114-132) Also available in print.
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Isolation of an ARGONAUTE gene in pelargonium and identification of candidate genes regulated through ARGONAUTE4-dependent RNA-dependent DNA methylation in Arabidopsis /He, Jie. January 2009 (has links)
Dissertation (Ph.D.)--University of Toledo, 2009. / Typescript. "Submitted as partial fulfillment of the requirements for the Doctor of Philosophy degree in Biology." Bibliography: leaves 54-56, 91-95, 118-119, 133-139.
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Large scale CpG island methylation profiling of small B cell lymphomaRahmatpanah, Farahnaz B. Caldwell, Charles W., January 2008 (has links)
The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Title from PDF of title page (University of Missouri--Columbia, viewed on April 1, 2010). Vita. Thesis advisor: Charles W. Caldwell. "May 2008" Includes bibliographical references
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Mechanical and geometric characterization of mouse cortical bone with osteoblast-specific knockout of insulin-like growth factor receptor geneRamaswamy, Girish. January 2007 (has links) (PDF)
Thesis (M.S.)--University of Alabama at Birmingham, 2007. / Title from PDF title page (viewed Sept. 23, 2009). Includes bibliographical references (p. 66-77).
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Curcumin inhibits cell migration of nasopharyngeal carcinoma through reactivation of e-cadherin expressionChan, Wing-san, January 2009 (has links)
Thesis (M. Phil.)--University of Hong Kong, 2009. / Includes bibliographical references (leaves 101-124) Also available in print.
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O⁶-methylguanine-DNA methyltransferase (MGMT) gene silencing using RNA interference and sensitivity to temozolomideDavis, Steven Michael, January 2008 (has links)
Thesis (M.S.)--Northern Michigan University, 2008. / "14-57395." Bibliography: leaves 41-42.
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Ras signalling pathway and MLL-rearranged leukaemias /Ng, Ming-him. January 2006 (has links)
Thesis (M. Phil.)--University of Hong Kong, 200. / Also available online.
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TransformaÃÃo genÃtica de feijÃo-caupi [Vigna unguiculata (L.) Walp] e tabaco (Nicotiana tabacum) com uma quitinase de classe I / Genetic transformation of cowpea [Vigna unguiculata (L.) Walp] and tobacco (Nicotiana tabacum) with a class I chitinaseEmmanuel de Sousa Jereissati 15 June 2012 (has links)
CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / A expressÃo heterÃloga de genes que codificam proteÃnas relacionadas à patogÃnse (PR-proteÃnas) representa uma alternativa promissora para o desenvolvimento de plantas resistentes ao ataque de fungos. Dentre as PR-proteÃnas com grande potencial biotecnolÃgico, estÃo as quitinases, hidrolases que catalisam a degradaÃÃo da quitina, que à um polÃmero constituinte da parede celular de muitas espÃcies de fungos fitopatogÃnicos. AlÃm da funÃÃo relacionada aos mecanismos de defesa, acredita-se que essas enzimas podem desempenhar outros papÃis, como a regulaÃÃo de processos de crescimento e desenvolvimento em plantas. O objetivo deste trabalho foi estudar o papel de uma quitinase de classe I de feijÃo-caupi (VuChiI) na defesa e no desenvolvimento vegetal e para isso duas abordagens foram conduzidas. A primeira consistiu no silenciamento do gene que codifica esta proteÃna em plantas de feijÃo-caupi. Nos experimentos de silenciamento gÃnico foram utilizados meristemas apicais de plÃntulas de feijÃo-caupi, que foram entÃo bombardeados com micropartÃculas portando um vetor de silenciamento (pKANNIBAL), onde foi clonada uma construÃÃo em grampo (intron harpin) do gene de VuChiI. As plantas regeneradas em meio de cultura de Murashige e Skoog (MS) contendo o herbicida imazapyr, como agente de seleÃÃo, foram analisadas por PCR, o que resultou na confirmaÃÃo da transformaÃÃo genÃtica de cinco plantas, a partir de 1.092 meristemas apicais bombardeados. O RNA foi extraÃdo das plantas transformadas e utilizado em anÃlises de Northern blot que possibilitou a detecÃÃo de siRNAs no RNA total extraÃdo das plantas putativamente transgÃnicas. Apenas uma das plantas transformadas produziu uma vagem contendo seis sementes, das quais apenas uma germinou. As plantas transformadas apresentaram ciclo de vida de mais de 365 dias enquanto que o esperado à de 65-70 dias. Com base nos resultados sugere-se que o silenciamento da quitinase de classe I de feijÃo-caupi pode ter promovido alteraÃÃo no desenvolvimento da planta. A segunda estratÃgia de transformaÃÃo genÃtica consistiu no co-cultivo de discos foliares de tabaco com suspensÃo de Agrobacterium tumefaciens LB4404, com o intuito de expressar a proteÃna recombinante no espaÃo extracelular das plantas regeneradas. Essa estratÃgia tem como objetivo fazer com que a proteÃna entre em contato com o fungo antes mesmo que este agente patogÃnico penetre na cÃlula vegetal. Para tanto, o inserto referente à quitinase de feijÃo-caupi foi clonado no vetor binÃrio pCAMBIA1305.2 sob controle do promotor CaMV35S e fundido ao peptÃdeo sinal GRP (glycine-rich protein de Oryza sativa), que permite a secreÃÃo da proteÃna recombinante via apoplasto. Foram produzidas trÃs linhagens de plantas que tiveram a transformaÃÃo genÃtica confirmada por PCR. As sementes obtidas das plantas transformadas, geraÃÃo R1, foram semeadas em meio seletivo para a anÃlise da transmissÃo do transgene. O teste de X2 indicou que o transgene foi transmitido para a progÃnie em proporÃÃes Mendelianas. Os modelos experimentais desenvolvidos neste trabalho poderÃo contribuir para a elucidaÃÃo dos papÃis biolÃgicos da quitinase de classe I de feijÃo-caupi. / Heterologous expression of genes encoding pathogenesis related proteins (PR-proteins) represents a promising alternative for the development of plants resistant to fungi. Among the PR-proteins with great biotechnological potentials are the chitinases, a class of hydrolases which catalyze the degradation of chitin, a polymer constituent of cell wall of several species of pathogenic fungi. Besides their function related to defense mechanisms, it is believed that these enzymes may play other roles, such as regulation of growth and development processes in plants. Using two approaches, this study sought to investigate the role of a class I chitinase of cowpea (VuChiI) in defense and plant development. The first approach consisted of silencing of the gene encoding for VuChiI in cowpea. Apical meristems excised from cowpea seeds were bombarded with microparticles containing a silencing vector (pKANNIBAL), which was cloned with a construct harboring an intron harpin of the gene VuChiI. Plants regenerated in a Murashige and Skoog (MS) media containing imazapyr as a selection agent, were analyzed by PCR, resulting in confirmation of genetic transformation of five plants from the 1092 apical meristems bombarded. Northern blot analysis of total RNA extracted from the putative transgenic plants allowed for the detection of siRNAs. Upon regeneration and germination, only one of the five transformed plants produced a pod containing six seeds. Of these, only one germinated when further planted. The transformed plants exhibited a life cycle of over 365 days, as against the normal cycle of 65-70 days. Based on these results we posit that silencing of class I cowpea chitinase may have led to changes in plant development. The second strategy consisted of co-cultivation of tobacco leaf discs with Agrobacterium tumefaciens strain LB4404 suspension to express recombinant protein in the extracellular space of the regenerated plants in order to expose the protein fungus even before its penetration into the plant cell. To achieve this, a sequence of cowpea chitinase insert was cloned into the binary vector pCAMBIA1305.2 under the control of CaMV35S promoter and signal peptide fused to glycine-rich protein of Oryza sativa (GRP), which allowed the secretion of recombinant protein via apoplast. This led to the generation of three strains as confirmed by PCR. Seeds obtained from transformed R1 generation of the plants were germinated and analyzed for transmission of the transgene using X2 test, which confirmed its transmission to the progeny in a Mendelian fashion. The experimental models developed in this work may serve to further assess the biological roles of class I chitinase from cowpea.
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Studies on the RNA interference pathway in mammalian cellsJagannath, Aarti January 2009 (has links)
No description available.
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