Thesis (Ph. D.)--University of Missouri-Columbia, 1999. / Typescript. Vita. Includes bibliographical references (leaves 72-79). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 1999. / Typescript. Vita. Includes bibliographical references (leaves 72-79). Also available on the Internet.

Thesis (Ph. D.)--University of Missouri-Columbia, 1996. / Typescript. Vita. Includes bibliographical references. Also available on the Internet.

Thesis (Ph.D.)--University of Western Australia, 2008.

Thesis (Ph. D.)--Ohio State University, 2005. / Title from first page of PDF file. Document formatted into pages; contains xv, 122 p.; also includes graphics. Includes bibliographical references (p. 117-122). Available online via OhioLINK's ETD Center

Thesis (Ph. D.)--Illinois State University, 2004. / Title from title page screen, viewed May 23, 2006. Dissertation Committee: Brian J. Wilkinson (chair), Radheshyam K. Jayaswal, Anthony J. Otsuka, David L. Williams, Wade A. Nichols. Includes bibliographical references (leaves 105-113) and abstract. Also available in print.

Thesis (Ph. D.)--Illinois State University, 2006. / Title from title page screen, viewed on June 8, 2007. Dissertation Committee: Kevin A. Edwards (chair), Craig Gatto, Wade A. Nichols, Samuel Galewsky, John C. Sedbrook. Includes bibliographical references (leaves 218-245) and abstract. Also available in print.

Thesis (doctoral)--Swedish University of Agricultural Sciences, 2000. / Thesis based on 4 papers, which are included in the volume. Includes bibliographical references.

Made available in DSpace on 2014-08-13T14:50:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-07-29Bitstream added on 2014-08-13T18:01:11Z : No. of bitstreams: 1 000736017_20140829.pdf: 268216 bytes, checksum: d24c916cb4f2f5d62140603bab304a45 (MD5) Bitstreams deleted on 2014-10-03T16:24:43Z: 000736017_20140829.pdf,Bitstream added on 2014-10-03T16:27:41Z : No. of bitstreams: 2 000736017_20140829.pdf.txt: 31163 bytes, checksum: e21dedff28a6a8d49196eb12ad922ee5 (MD5) 000736017.pdf: 1092117 bytes, checksum: 23730ac0c228da8c6df28821beccf960 (MD5) Bitstreams deleted on 2014-10-03T16:33:17Z: 000736017.pdf,Bitstream added on 2014-10-03T16:43:31Z : No. of bitstreams: 2 000736017_20140829.pdf.txt: 31163 bytes, checksum: e21dedff28a6a8d49196eb12ad922ee5 (MD5) 000736017.pdf: 1092117 bytes, checksum: 23730ac0c228da8c6df28821beccf960 (MD5) Bitstreams deleted on 2014-10-03T16:48:57Z: 000736017.pdf,Bitstream added on 2014-10-03T16:49:48Z : No. of bitstreams: 1 000736017.pdf: 1092117 bytes, checksum: 23730ac0c228da8c6df28821beccf960 (MD5) Bitstreams deleted on 2014-10-27T11:47:13Z: 000736017.pdf,Bitstream added on 2014-10-27T11:48:06Z : No. of bitstreams: 1 000736017.pdf: 1092117 bytes, checksum: 23730ac0c228da8c6df28821beccf960 (MD5) / As β-defensinas são as proteínas mais amplamente estudadas dentre as três subfamílias das defensinas, uma vez que possuem extensa atividade antimicrobiana e são produzidas por vários tipos de células epiteliais, fornecendo assim proteção às membranas das células do hospedeiro. Em mamíferos, além de sua atividade antimicrobiana, essas proteínas atuam na maturação do espermatozoide, na capacitação espermática, na proteção do espermatozoide contra a resposta imune da fêmea e na expressão diferenciada e específica nos tecidos reprodutivos, sugerindo assim, uma importante função na reprodução e na fertilidade. O painel de linhagens celulares híbridas irradiadas (painel RH) construído para o genoma do búfalo (BBURH5000) vem sendo utilizado com sucesso nos estudos de mapeamento dos cromossomos bubalinos. A estratégia do mapeamento RH, ou seja, a utilização do painel RH associado a análises estatísticas, determina a ordem linear de marcadores nos cromossomos, sendo uma importante ferramenta para a construção de mapas físicos de alta resolução do genoma, e também na construção de mapas comparativos entre espécies. O presente trabalho teve por objetivo utilizar o painel BBURH5000 para mapear um conjunto de genes das β-defensinas no cromossomo 14 bubalino (BBU14) e a construção de um mapa comparativo in silico entre o BBU14 e seu homólogo em bovino. Para tal, foram testados com DNA bubalino, sequências de iniciadores para PCR provenientes de oito genes das β-defensinas, localizados no cromossomo 13 bovino (BTA13), o qual é homólogo ao BBU14. Do total de marcadores testados, seis amplificaram produtos de PCR adequados ao mapeamento utilizando o painel BBURH5000. No entanto, dois marcadores foram excluídos na etapa de genotipagem. A frequência de retenção dos marcadores no painel apresentou uma variação de 16,66% com o marcador BBD125 a 20,00% com ... / The β-defensins are proteins most widely studied of the three subfamilies of defensins, because have extensive antimicrobial activity and are produced by several types of epithelial cells, thereby providing protection to the membranes of host cells. In mammals, in addition to their antimicrobial activity, these proteins act in the maturation of spermatozoa, in sperm capacitation, in protecting the sperm against the female immune response and specific and differential expression in reproductive tissues, thus suggesting an important role in reproduction and fertility. The radiation hybrid panel (RH panel) constructed for buffalo genome (BBURH5000) has been successfully used in mapping studies of buffalo chromosomes. The RH mapping strategy, in other words, use of RH panel associated statistical analysis, determines the linear order of markers on chromosomes and is an important tool for the construction of physical maps of high resolution genome and also in the construction of comparative maps between species. The present study has the purpose to use the BBURH5000 panel to map a set of β-defensins genes on buffalo chromosome 14 and the construction of a comparative map in silico between BBU14 and homologous bovine. To this end, we tested buffalo DNA sequences of primers for PCR from eight genes of β-defensins, located on bovine chromosome 13 (BTA13), which is homologous to BBU14. Of the markers tested, six amplified suitable PCR product to the mapping using BBURH5000 panel. However, two markers were excluded in step genotyping. The retention frequency of markers on the panel presented a variation of 16.66% with a marker BBD125 to 20.00% with markers BBD119 and BBD122. The comparative analysis in silico between the BBU14 RH map and the sequence map of BTA13 allowed to indicate the location of these markers on buffalo chromosome 14

Orientador: Fausto Foresti / Coorientador: José Carlos Pansonato Alves / Banca: Teruo Hashimoto / Banca: Marcelo Ricardo Vicari / Resumo: Complexos de espécies crípticas são grupos de espécies estreitamente relacionadas, dificilmente distinguíveis por características morfológicas. Estes complexos são conhecidos em uma significativa variedade de organismos, principalmente plantas, insetos, fungos e peixes. Em peixes, complexos de espécies já foram identificados para representantes de diferentes ordens, como Characiformes, Gymnotiformes e Synbranchiformes. No presente estudo, foram analisadas amostras de S. marmoratus coletadas em oito localidades brasileiras distintas com o uso de técnicas citogenéticas clássicas (coloração convencional com Giemsa, localização das regiões organizadoras de nucléolo pela marcação com nitrato de Prata - AgNO3 e bandamento C) e moleculares (hibridação in situ fluorescente com sondas de DNAr 5S e 18S, DNAhis H3, elemento transponível Rex3 e sondas teloméricas). Além disso, análises moleculares foram realizadas utilizando dados de sequências de três genes mitocondriais (Citocromo B - CytB, Citocromo oxidase 1 - COI e DNAr 16S) de indivíduos com cariótipo conhecido. Considerando as amostras analisadas, números diploides distintos foram encontrados (42, 44 e 46 cromossomos) que, combinados com as fórmulas cariotípicas, resultam na distinção de cinco cariomorfos em S. marmoratus. A análise filogenética realizada confirma o status de complexo de espécies e revela que eventos múltiplos, possivelmente bidirecionais, seriam responsáveis pelo surgimento da variabilidade cariotípica neste grupo. O mapeamento físico do DNAr 5S revela que estes sítios se mantiveram conservados durante a história evolutiva de Synbranchus, enquanto que, de forma oposta, os sítios para DNAr 18S estão distribuídos de maneira polimórfica, com variações intrapopulacionais significativas, indicando que mecanismos evolutivos distintos estão agindo sobre a dispersão destas sequências. O mapeamento de ... / Abstract: Cryptic species complex are groups of species straightly related but hardly distinguished by morphological traits. These complexes are known to occur in a great variety of organisms, mainly plants, insects, yeasts and also fishes. Considering the fish group, species complex had already been identified in representatives from different orders, such as Characiformes, Gymnotiformes e Synbranchiformes. In the present study, samples of S. marmoratus collected at eight different Brazilian sites were analyzed, using classical cytogenetic techniques (conventional staining with Giemsa, localization of nucleolar organizer regions with Silver nitrate staining - AgNO3 and C-banding) and molecular (Fluorescence in situ hybridization with 5S and 18S rDNA, H3 DNAhis, transposable element Rex3 and telomeric probes). Besides that, molecular analyses were carried out using sequence data from three mitochondrial genes (Cytochrome B - CytB, Cytochrome oxidase 1 - COI and 16S) obtained for each individual with known karyotype. Considering the analyzed samples, distinct diploid numbers were found (42, 44 and 46 chromosomes) and, associated with karyotype formulae, resulted in the distinction of five karyomorphs in S. marmoratus. The phylogenetic analyses confirmed the status of a species complex and revealed that multiple and, possibly bidirectional events were responsible the karyotypic variability found in this group. The physical mapping of 5S rDNA reveals that these sites kept conserved during the evolutionary history of Synbranchus, contrary to 18S rDNA sites which are distributed in a polymorphic way, with sigficant intrapopulational variability, indicating that distinct evolutionary mechanisms may be acting upon the dispersion of these sequences. The mapping of H3 hisDNA revealed a dispersed pattern of distribution of these sites on the genome of all karyomorphs, frequently found as little clusters accumulated ... / Mestre