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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
21

Simulation of Mathematical Models in Genetic Analysis

Patel, Dinesh Govindal 01 January 1964 (has links)
In recent years a new field of statistics has become of importance in many branches of experimental science. This is the Monte Carlo Method, so called because it is based on simulation of stochastic processes. By stochastic process, it is meant some possible physical process in the real world that has some random or stochastic element in its structure. This is the subject which may appropriately be called the dynamic part of statistics or the statistics of "change," in contrast with the static statistical problems which have so far been the more systematically studied. Many obvious examples of such processes are to be found in various branches of science and technology, for example, the phenomenon of Brownian Motion, the growth of a bacterial colony, the fluctuating numbers of electrons and protons in a cosmic ray shower or the random segregation and assortment of genes (chemical entities responsible for governing physical traits for the plant and animal systems) under linkage condition. Their occurrences are predominant in the fields of medicine, genetics, physics, oceanography, economics, engineering and industry, to name only a few scientific disciplines. The scientists making measurements in his laboratory, the meteriologist attempting to forecast weather, the control systems engineer designing a servomechanism (such as an aircraft or a thermostatic control), the electrical engineer designing a communication system (such as the radio link between entertainer and audience or the apparatus and cables that transmit messages from one point to another), economist studying price fluctuations in business cycles and the neurosurgion studying brain wave records, all are encountering problems to which the theory of stochastic processes may be relevant. Let us consider a few of these processes in a little more detail. In statistical physics many parts of the theory of stochastic processes were developed in correlation with the study of fluctuations and noise in physical systems (Einstein, 1905; Smoluchowski, 1906; and Schottky, 1918). Consequently, the theory of stochastic processes can be regarded as the mathematical foundation of statistical physics. The stochastic models for population growth consider the size and composition of a population which is constantly fluctuating. These are mostly considered by Bailey (1957), Bartlett (1960), and Bharucha-Reid (1960). In communication theory a wide variety of problems involving communication and/or control such as the problem of automatic tracking of moving objects, the reception of radio signals in the presence of natural and artificial disturbances, the reproduction of sound and images, the design of guidance systems, the design of control systems for industrial processes may be regarded as special cases of the following general problem; that is, let T denote a set of points in a time axis such that at each point t in T an observation has been made of a random variable X(t). Given the observations [x(t), t ϵT] and a quantity Z related to the observation, one desires to from in an optimum manner, estimates of, and tests of hypothesis about Z and various functions h(Z).
22

Associação de escores de condição corporal com características reprodutivas de vacas Nelore e com desempenho de seus bezerros

Fernandes, Anna Flávia de Araujo [UNESP] 28 February 2012 (has links) (PDF)
Made available in DSpace on 2014-06-11T19:26:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-02-28Bitstream added on 2014-06-13T18:29:27Z : No. of bitstreams: 1 fernandes_afa_me_jabo.pdf: 322816 bytes, checksum: d60d045e58509fb91c9a8dc47345094d (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / No Brasil, são escassos os estudos referentes ao escore de condição corporal (ECC) de vacas de corte que, quando associado ao peso, têm impacto na produção de bezerros e na reprodução, pois reflete o balanço energético do corpo animal. Assim, o objetivo deste trabalho foi associar ECC com a prenhez de vacas Nelore e com o desempenho de seus produtos a desmama, bem como verificar se esta característica pode ser incluída nos critérios de seleção da raça Nelore. O ECC das vacas foi atribuído no diagnóstico de gestação ou no desmame, variando de 1 a 5. Por regressão logística, utilizando o método de seleção Stepwise, foram analisados os seguintes efeitos sobre o ECC: grupo de contemporâneos a desmama, peso e altura de garupa da vaca, ordem de parto, diagnóstico de gestação, histórico reprodutivo anterior à coleta do ECC, presença de bezerro ao pé e escores de C e P dos produtos a desmama. Na análise genética, elaborada usando-se inferência baeysiana, foram incluídos os mesmos efeitos anteriores, com peso e altura de garupa da vaca como co-variáveis, e acrescidos do efeito genético aditivo da vaca. Os resultados mostraram que vacas com ECC elevado emprenharam mais do que as de pior ECC, porém o desempenho dos seus bezerros foi inferior. Vacas multíparas foram mais pesadas e de escores mais magros (ECC 1 e 2). As correlações de Spearman entre ECC e suas fontes de variação foram, em sua maioria, de baixa magnitude. Os efeitos que mais explicaram a variação do ECC foram peso, grupo de contemporâneos e altura da garupa. A estimativa de herdabilidade do ECC foi igual a 0,22, com intervalo de credibilidade entre 0,13 e 0,31, valor de magnitude moderada, demonstrando a presença de ação gênica aditiva na determinação do ECC e que este pode ser utilizado como critério de seleção de vacas / In Brazil few studies have been done on the body condition score (BCS) of beef cows. The knowledge of this score associated with body weight can produce an impact on calf production and cow´s reproduction, because it reflects the energy balance of the animal body. The objective of this study was to associate BSC with pregnancy of cows and the performance of their calves at weaning, and to verify if this trait could be included as selection criteria. The BCS was recorded on pregnancy diagnosis or at weaning and ranged from 1 to 5. Applying logistic regression, using the Stepwise selection method, we analyzed the following effects on BSC: contemporary group at weaning, classes of weight and hip height of the cow, number of calvings, pregnancy diagnosis, reproductive history prior to collection BSC, the presence of calf and C and P score of calves at weaning. For the genetic analysis, performed using Baesyan inference, the same effects were considered, with weight and hip height as covariate, and also the genetic direct effect of the cow. The results showed that cows with higher BCS had better pregnancy rate than those with lower BSC, but the performance of their calves was worse. Multiparous cows were heavier and had leaner scores (BCS 1 and 2) than the primiparous ones. The Spearman rank correlation between BSC and their sources of variation were generally of low magnitude. Contemporary group, body weight and height of the cow were the effects that accounted for most of the variation of BSC. The heritability estimate of BCS was equal to 0.22 with credible interval ranging from 0.13 to 0.31, a moderate magnitude value that indicates the presence of additive genetic variation and that BSC can be used as selection criterion for cows
23

Genetic Analysis of Medicago truncatula Plants with a Defective MtIRE Gene

Alexis, Naudin 08 1900 (has links)
Leguminous plants are able to fix nitrogen by establishing a symbiotic relationship with soil dwelling bacteria, called rhizobia. The model plant Medicago truncatula forms a partnership with Sinorhizobium meliloti whereby the plant gains bioavailable nitrogen and in exchange the bacteria gains carbohydrates. This process occurs within nodules, which are structures produced on the roots of the plants within which nitrogen is fixed. M. truncatula incomplete root elongation (MtIRE) was localized to the infection zone, which is zone II of indeterminate nodules. It was shown to encode a signaling kinase so it was anticipated to play a role in nodulation. Mutants of MtIRE in the R108 background, mutagenized with the Tnt1 retrotransposon, were obtained from reverse screen, and were assessed to determine if a disrupted MtIRE gene was the cause of nitrogen fixation defective nodules. Mutant line NF1320, having a mutant phenotype, showed typical Mendelian segregation of 3:1 when backcrossed to R108. Experimental results show that MtIRE gene is not the cause of the mutant phenotype, but was linked to the causative locus. MtIRE co-segregated with the mutant phenotype 83%. Southern blot and the first version of the M. truncatula genome (version 3.5) reported a single MtIRE gene and this was shown to be on chromosome 5 but the latest version of the M. truncatula genome (version 4.0) showed a second copy of the gene on chromosome 4. The genome sequence is based on the A17 reference genome. Both genes are 99% identical. Genetic markers that originate from flanking sequence tags (FSTs) on both chromosome 4 and 5 were tested in an attempt to find an FST that co-segregated with the mutant phenotype 100%. An FST derived from a Tnt1 insertion in Medtr4g060930 (24F) co-segregated with the mutant phenotype closely, with 76% co-segregation. Medtr4g060930 (24F) is on chromosome 4, making it likely that the Tnt1 inserted in the MtIRE gene is also on chromosome 4, and thus the defective gene is on chromosome 4.
24

シアノバクテリアにおける高頻度なin vivoのトランスポゾンタギング系の開発およびその系を利用したChl dを利用するシアノバクテリア、Acaryochloris marinaにおける順遺伝学的解析の確立 / Development of a high-frequency in vivo transposon mutagenesis system for cyanobacteria and establishment of the forward genetic analysis of the Chl d-dominated cyanobacterium, Acaryochloris marina by use of the system

渡部, 和幸 23 March 2015 (has links)
Kyoto University (京都大学) / 0048 / 新制・課程博士 / 博士(人間・環境学) / 甲第19069号 / 人博第722号 / 新制||人||173 / 32020 / 京都大学大学院人間・環境学研究科相関環境学専攻 / (主査)准教授 土屋 徹, 教授 宮下 英明, 教授 川本 卓男 / 学位規則第4条第1項該当
25

The analysis of starch degradation in Solanaceae species

Samodien, Mugammad Ebrahim 04 1900 (has links)
Thesis (PhD)--Stellenbosch University, 2014. / ENGLISH ABSTRACT: This project involved the analysis of genes in Solanaceae species that have previously been shown to be involved in the phosphorylation of starch or its subsequent dephosphorylation. Both these processes are essential for normal starch mobilization. A tomato conditional mutant lacking the starch phosphorylating enzyme glucan water dikinase was analyzed. It is known that starch accumulates transiently in tomato fruit and is degraded throughout the ripening process. The study aimed to determine the effect of inhibited starch degradation on fruit development. Unfortunately no effect on starch mobilisation was found in the fruit of the mutant. Immunoblot analysis revealed expression of Glucan Water Dikinase (GWD) within the fruit of the tomato mutant indicating that the conditionality of the mutation was compromised. The second set of experiments analyzed the roles of Starch Excess4 (SEX4), Like Sex Four-1 and Like Sex Four-2 (LSF1 and LSF2) in starch degradation in potato and Nicotiana benthamiana. These enzymes have, thus far, only been studied in Arabidopsis, with the proposed role for SEX4 and LSF2 being that they are involved in dephosphorylation of the C-6 and C-3 positions of starch breakdown products. The role of LSF1 is unclear, although it is not thought to be a phosphatase. SEX4, LSF1 and LSF2 were repressed individually while the expression of SEX4 and LSF2 were also inhibited simultaneously. Using a transient repression system in N. benthamiana it was shown that all of the genes play a role in leaf starch degradation. The SEX4 and LSF2 enzymes were shown to influence the proportion of phosphate located on the starch which contained an altered ratio of C-3/C-6 phosphate. Stably transformed potato plants were produced where SEX4 and LSF2 were successfully repressed in potato leaves and tubers. Although AtLSF2 had been shown not to be essential for normal starch degradation on its own, in potato plants when LSF2 was repressed, the plants developed a starch-excess phenotype. Taken together with the N. benthamiana data this indicates that LSF2 plays a bigger role in leaf starch degradation in Solanaceae than in Arabidopsis. The ratio of C-3/C-6 phosphate was also altered in tuber starch from some of the silenced plants. Starch from SEX4 repressed potato plants contained increased amounts of glucose-6-phosphate and increased glucose-3-phosphate in the tuber when compared to the WT. An increase in the proportion of C-6 or C-3 phosphate is not surprising with SEX4 being characterized as a phosphatase specific for C-6 position and LSF2 for the C-3 position in Arabidopsis, however the combined increase in C-3 and C-6 amounts in StSEX4 silenced plants is interesting. The differences seen in the phosphate alteration in both N. benthamiana leaves and potato tubers indicates that in Solanaceae species these proteins may have a slightly altered specificity when compared with Arabidopsis, although they are undoubtedly involved in starch degradation. The effect of silencing SEX4 or LSF2 on cold-induced sweetening was also investigated, with no effect being found. This may be because of functional redundancy between the proteins and a better approach in terms of blocking cold sweetening would be to simultaneously repress SEX4 and LSF2. Overall, these enzymes seem to play similar roles in leaves of Solanum species as has been described in Arabidopsis. The starch from the engineered plants did have an altered phosphate ratio and further analysis is needed to determine if this leads to improved or additional functionality. / AFRIKAANSE OPSOMMING: Die projek omhels die ontleding van gene van die Solanaceae spesie wat voorheengetoon het dat hulle deel neem in fosforilering of defosforilering van stysel. Altwee van hierdie reaksies is belangrik vir normale stysel metabolisme. ‘n Tamatie konditionele mutant was geanaliseer waarin die stysel fosforilering ensiem glucan water dikinase nie teenwoordig was nie. Die doel van die studie was om te ondersoek watter effek het n gebrek in stysel afbraak op die rypwording en ontwokkeling vrugte. Ongelukkig was geen effek op stysel metabolism in die munant se vrugte gesien. Immunoklad analise het getoon dat GWD protein wel uitdruk word in die vrugte en dus die mutant nie heeltemal effektief was nie. Die tweede stel van experimente het in aartappels en tabak die rol van SEX4, LSF1 en LSF2 in stysel afbraak ondersoek. Hierdie ensieme was huidiglik nog net deeglik in Arabidopsis bestudeer, waar daar gewys was dat SEX4 and LSF2 in die defosforilering van stysel by die C-6 en C-3 posisie deel neem. Die rol van LSF1 is nog onbekend, maar daar word huiglik gelgo dat dit is nie ‘n fosfatase nie. SEX4, LSF1, en LSF2 was onderdruk op sy eie, waar SEX4 en LSF2 gelyktydig onderdruk was. Met behulp van n verbygaande onderdrukking in tabak, was dit getoon dat al die bogenoemde gene n gedeeltelike rol speel in die afbraak van stysel. Dit was getoon dat SEX4 and LSF2 ensiemedie verhouding van waar fosfaat op stysel gelee is beinvloed en het n verandering in die C-3/C-6 phosphaat verhouding ook gehad. Aardappels was stabiel getransformeer en daar was suksesfol plante waar SEX4 en LSF2 onderdruk was in blare en knolle geproduseer. Alhoewel daar getoon was dat AtLSF2 op sy eie nie n groot rol speel in stysel katabolisme nie was daar wel gesien dat in aardappel wanner hierdie geen afgeskakel was dat daar n stysel oorskot fenotiepe ontwikkel. As die tabak resultate saamgevat word met die aardappel wil dit voorkom asof LSF2 n groter rol binne die stysel katabolisme in Solanaceae speel as in Arabidopsis. Daar was gevind dat die verhouding van C-3/C-6 fosfaat was in die knolle verander in perty van die lyne waar geen afskakeling wel plaasgevind het. Die verhouding van C-3/C-6 fosfaat was verander in knolle stysel van sommige stilgemaak plante. Sysel van SEX4 stilgemaak plante het hoër vlakke glukose-6-fosfaat en glukose-3-fosfaat in die knolle gehad wanner dit met die WT vergelyk was. n Toename in die persentasie van C-6 fosfaat is nie verbasend nie, SEX4 word gekenmerk as die spesifieke fosfatase verantwoordelik vir die fosfaat by die C-6 posisie en LSF2 spesifiek vir die C-3 posisie in Arabidopsis. Die gekombineerde toename in beide C-6 en C-3 bedrae in StSEX4 stilgemaak plante is wel heel interesant. Verandering in beide tabak blare and aartapple knolle dui daarop dat in solanacea spesie hierdie proteiene, n effens verandering in spesifisiteit kan hê as dit met Arabidopsis vergelyk word. Daar kan wel nie getwyfel word dat hulle wel n rol speel in stysel afbraak nie. Die effect watSEX4 of LSF2 op koue-geinduseerde soetheid het is ook ondersoek maar daar was geen effek gevind nie. Dit mag wees asgevolg van die funksionele onslag tussen die twee proteien en better benadering on die koue-soetheids effek te verhoed sou wees om beide protein op die selfde stadium aft e skakel. As daar in gegeheel gekyk word lyk dit asof hierdie protein die selfde rolle het in die Solanum spesies as in Arabidopsis.Die stysel van hierdie die ontwerpte plante het ‘n veranderde fosfaat verhouding getoon en veder analise is nodig om te bepaal of dit lei tot verbeterde einskappe of bykommende funksies.
26

Κυτταρολογική χαρτογράφηση γονιδίων και ανώνυμων DNA κλώνων-μοριακή ανάλυση της πολυγονιδιακής οικογένειας hsp70 στη μεσογειακή μύγα ceratitis capitata

Κρητικού, Δήμητρα 24 March 2010 (has links)
- / -
27

Análise filogenética de diferentes populações do carrapato Rhipicephalus sanguineus provenientes de diferentes regiões do Brasil, da América Latina, Espanha, Itália e África do Sul / Genetic Analysis of different populations of Rhipicephalus sanguineus from Brazil, Latin America, Spain, Italy and South Africa

Jonas Moraes-Filho 26 February 2010 (has links)
Dentro do gênero Rhipicephalus, há o chamado complexo sanguineus formado por várias espécies presentes apenas no Velho Mundo, das quais apenas a espécie Rhipicephalus sanguineus é considerada de ocorrência no Novo Mundo. Como as espécies deste complexo apresentam grande similaridade morfológica, é possível que mais de uma espécie do complexo sanguineus esteja ocorrendo nas Américas. Um trabalho recente relatou parâmetros biológicos e genéticos significativamente diferentes entre uma população de R. sanguineus do Brasil e outra da Argentina, sugerindo que o status biosistemático de R. sanguineus na América do Sul deva ser revisto. Diante disto, e da inquestionável importância medico-veterinária de R. sanguineus na América do Sul, este projeto objetivou realizar uma análise genética de 32 populações de R. sanguineus, sendo 17 do Brasil (englobando 13 estados), 3 do Chile, 2 da Venezuela, 2 da Colômbia e 1 de cada um dos seguintes países: Argentina, Uruguai, Itália, Espanha, África do Sul, Costa Rica, Panamá, México. Uma amostra de Rhipicephalus turanicus da Espanha e uma da África do sul também foram analisadas. Para tal, carrapatos oriundos das diferentes populações foram analisados geneticamente através de seqüências dos genes mitocondriais 16S rDNA. O resultado deste trabalho permitiu inferir sobre a possibilidade da existência no mínimo de dois grupos distintos de carrapatos sob o táxon R. sanguineus na América Latina, um se aproximando dos carrapatos de origem africana e com distribuição na América tropical e subtropical e outro se aproximando das amostras européias e com distribuição temperada do sul da América do Sul / Among the genus Rhipicephalus, there is the sanguineus complex composed by many species of distribution restricted to the Old World. It has been considered that Rhipicephalus sanguineus is the only species from this complex to be present in the New World. Due to the high similarity between the sanguineus complex species, it is possible that more than one species is present in the Americas, as suggested by recent papers. The present study performed a genetic evaluation based on 16S rDNA mitochondrial gene sequences of ticks representative 26 R. sanguineus populations from South America, one from: Italy, Spain, South Africa, Costa Rica, Panama, Mexico. Additionally, Rhipicephalus turanicus ticks from South Africa and Spain were also used. The results confirm the existence of genetically distinct populations of the \"sanguineus\" complex in the Americas
28

Bayesian Lasso for Detecting Rare Genetic Variants Associated with Common Diseases

Zhou, Xiaofei 23 October 2019 (has links)
No description available.
29

An agile model-driven method for involving end-users in DSL development

Villanueva del Pozo, María José 25 January 2016 (has links)
[EN] Domain-specific languages (DSLs) are considered to be a powerful tool for enhancing the efficiency of software developers and bring software development closer to end-users from complex domains. However, the successful development of a DSL for a complex domain is a challenge from the technical point of view and because end-user acceptance is key. Despite this fact, the relevant role of end-users during DSL development has traditionally been neglected. Normally, end-users participate at the beginning to communicate their preferences but they do not participate again until the DSL is completely implemented. As a consequence, if the language to develop reaches a complex domain, the chances that errors appear in the DSL are higher and solving them could involve large modifications that could have been avoided. As a solution, in this PhD thesis, we propose an agile, model-driven method to involve end-users in DSL development. This thesis researches if the combination of best practices from the model-driven development (MDD) discipline and best practices from agile methods is a suitable approach to involve end-users in the DSL development process. In order to validate the proposal, we have selected a highly complex domain such as the genetic analysis domain and we have collaborated with geneticists from three organizations. The proposed method has been used to involve these geneticists in the development of a DSL for the creation of genetic analysis pipelines. Simultaneously, we have carried out an empirical experiment to validate whether end-users and developers were satisfied with the proposal. / [ES] Los lenguajes específicos de dominio (DSLs) son una herramienta muy potente para mejorar la eficiencia de los desarrolladores de software, así como para acercar el desarrollo software a usuarios sin conocimientos informáticos. Sin embargo, su principal problema es que desarrollar un DSL es complejo; no sólo desde el punto de vista técnico, sino especialmente porque la aceptación de dicho lenguaje por parte de los usuarios finales es clave. A pesar de este hecho, los métodos tradicionales de desarrollo de DSLs no enfatizan el importante rol de los usuarios finales durante el desarrollo. Normalmente, los usuarios participan al inicio para comunicar sus preferencias, pero no vuelven a participar hasta que el DSL está completamente desarrollado. Si el lenguaje a desarrollar aborda un dominio complejo, la posibilidad de que existan errores en el DSL es mayor, y su solución podría conllevar a modificaciones de gran calibre que podrían haberse evitado. Como solución, en esta tesis proponemos un método de desarrollo de DSLs, ágil, y dirigido por modelos que involucra a los usuarios finales. Esta tesis investiga si la combinación de buenas prácticas del desarrollo dirigido por modelos (MDD) y de buenas prácticas de métodos ágiles es adecuada para involucrar a los usuarios finales en el desarrollo de DSLs. Para validar la idoneidad de la propuesta, se ha seleccionado un dominio complejo como el de los análisis genéticos y se ha colaborado con un conjunto de genetistas procedentes de tres organizaciones. El método propuesto se ha utilizado para involucrar a dichos genetistas en el desarrollo de un DSL para la creación de pipelines para el análisis genético. Conjuntamente, se ha llevado a cabo un experimento empírico para validar si los usuarios finales y los desarrolladores están satisfechos con la propuesta de la presente tesis. En resumen, las contribuciones principales de esta tesis doctoral son el diseño e implementación de un método innovador, ágil y dirigido por modelos para involucrar a los usuarios finales en el desarrollo de DSLs, así como la validación de dicha propuesta en un entorno industrial en un desarrollo real de un DSL. / [CAT] Els llenguatges específics de domini (DSLs) son una ferramenta molt potent per a millorar l'eficiència dels desenvolupadors de programari, així com per a apropar el desenvolupament de programari a usuaris sense coneixements informàtics. El problema es que desenvolupar un DSL es complex, no sols des del punt de vista tècnic, sinó especialment perquè l'acceptació de dit llenguatge per part dels usuaris finals es clau. Malgrat aquest fet, els mètodes tradicionals de desenvolupament de DSLs no emfatitzen l'important rol dels usuaris finals durant el desenvolupament. Normalment, els usuaris participen a l'inici per a comunicar les seues preferències, però no tornen a participar fins que el DSL està completament desenvolupat. Si el llenguatge a desenvolupar aborda un domini complex, la possibilitat de que hi hagen errors en el DSL es major i solucionar-los podria implicar modificacions de gran calibre que podrien haver-se evitat. Com a solució, en aquesta tesis proposem un mètode de desenvolupament de DSLs, àgil i dirigit per models que involucra als usuaris finals. Aquesta tesis investiga si la combinació de bones pràctiques del desenvolupament dirigit per models (MDD) i de bones pràctiques de mètodes àgils es adequada per a involucrar els usuaris finals en el desenvolupament de DSLs. Per a validar la idoneïtat de la proposta, s'ha seleccionat un domini complex com el dels anàlisis genètics i s'ha col·laborat amb un conjunt de genetistes procedents de tres organitzacions. El mètode s'ha utilitzat per a involucrar a dits genetistes en el desenvolupament d'un DSL per a la creació de pipelines per al anàlisis genètic. Al mateix temps, s'ha dut a terme un experiment empíric per a validar si tant els usuaris finals com els desenvolupadors estan satisfets amb la proposta de la present tesis. En resum, les contribucions principals d'aquesta tesis doctoral son el disseny i implementació d'un mètode innovador, àgil i dirigit per models per a involucrar als usuaris finals en el desenvolupament de DSLs, així com la validació de la proposta en un entorn industrial amb un desenvolupament real d'un DSL. / Villanueva Del Pozo, MJ. (2016). An agile model-driven method for involving end-users in DSL development [Tesis doctoral no publicada]. Universitat Politècnica de València. doi:10.4995/Thesis/10251/60156. / TESIS
30

Análise filogenética de diferentes populações do carrapato Rhipicephalus sanguineus provenientes de diferentes regiões do Brasil, da América Latina, Espanha, Itália e África do Sul / Genetic Analysis of different populations of Rhipicephalus sanguineus from Brazil, Latin America, Spain, Italy and South Africa

Moraes-Filho, Jonas 26 February 2010 (has links)
Dentro do gênero Rhipicephalus, há o chamado complexo sanguineus formado por várias espécies presentes apenas no Velho Mundo, das quais apenas a espécie Rhipicephalus sanguineus é considerada de ocorrência no Novo Mundo. Como as espécies deste complexo apresentam grande similaridade morfológica, é possível que mais de uma espécie do complexo sanguineus esteja ocorrendo nas Américas. Um trabalho recente relatou parâmetros biológicos e genéticos significativamente diferentes entre uma população de R. sanguineus do Brasil e outra da Argentina, sugerindo que o status biosistemático de R. sanguineus na América do Sul deva ser revisto. Diante disto, e da inquestionável importância medico-veterinária de R. sanguineus na América do Sul, este projeto objetivou realizar uma análise genética de 32 populações de R. sanguineus, sendo 17 do Brasil (englobando 13 estados), 3 do Chile, 2 da Venezuela, 2 da Colômbia e 1 de cada um dos seguintes países: Argentina, Uruguai, Itália, Espanha, África do Sul, Costa Rica, Panamá, México. Uma amostra de Rhipicephalus turanicus da Espanha e uma da África do sul também foram analisadas. Para tal, carrapatos oriundos das diferentes populações foram analisados geneticamente através de seqüências dos genes mitocondriais 16S rDNA. O resultado deste trabalho permitiu inferir sobre a possibilidade da existência no mínimo de dois grupos distintos de carrapatos sob o táxon R. sanguineus na América Latina, um se aproximando dos carrapatos de origem africana e com distribuição na América tropical e subtropical e outro se aproximando das amostras européias e com distribuição temperada do sul da América do Sul / Among the genus Rhipicephalus, there is the sanguineus complex composed by many species of distribution restricted to the Old World. It has been considered that Rhipicephalus sanguineus is the only species from this complex to be present in the New World. Due to the high similarity between the sanguineus complex species, it is possible that more than one species is present in the Americas, as suggested by recent papers. The present study performed a genetic evaluation based on 16S rDNA mitochondrial gene sequences of ticks representative 26 R. sanguineus populations from South America, one from: Italy, Spain, South Africa, Costa Rica, Panama, Mexico. Additionally, Rhipicephalus turanicus ticks from South Africa and Spain were also used. The results confirm the existence of genetically distinct populations of the \"sanguineus\" complex in the Americas

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