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11	Functional Genetic Analysis Reveals Intricate Roles of Conserved X-box Elements in Yeast Transcriptional Regulation Voll, Sarah January 2013 (has links) Understanding the functional impact of physical interactions between proteins and DNA on gene expression is important for developing approaches to correct disease-associated gene dysregulation. I conducted a systematic, functional genetic analysis of protein-DNA interactions in the promoter region of the yeast ribonucleotide reductase subunit gene RNR3. I measured the transcriptional impact of systematically perturbing the major transcriptional regulator, Crt1, and three X-box sites on the DNA known to physically bind Crt1. This analysis revealed interactions between two of the three X-boxes in the presence of Crt1, and unexpectedly, a significant functional role of the X-boxes in the absence of Crt1. Further analysis revealed Crt1- independent regulators of RNR3 that were impacted by X-box perturbation. Taken together, these results support the notion that higher-order X-box-mediated interactions are important for RNR3 transcription, and that the X-boxes have unexpected roles in the regulation of RNR3 transcription that extend beyond their interaction with Crt1. functional genetic analysis transcription factor (TF) cis-regulatory element (CRE) binary interaction higher order interaction multiple linear regression additive multiplicative synthetic genetic array (SGA) green fluorescent protein (GFP) ribonucleotide reductase (RNR) yeast X-box transcriptional regulation RNR3
12	Ultraschnelle, lichtinduzierte Primärprozesse im elektronisch angeregten Zustand des Grün Fluoreszierenden Proteins (GFP) / Ultrafast Elementary Events in the Excited State of Green Fluorescent Protein (GFP) Winkler, Kathrin 24 January 2003 (has links) No description available. 540 Chemie Mathematics and Computer Science Grün Fluoreszierendes Protein (GFP) Pump-Probe-Spektroskopie Protonentransfer ultraschnell innere Konversion Schwingungsenergietransfer Green Fluorescent Protein (GFP) pump-probe-spectroscopy excited state proton transfer ultrafast internal conversion vibrational energy transfer 35.13 35.25 35.16 SIL 000: Laser-Chemie {Strahlungschemie} SGF 400 RRQ 000: Laser-Spektroskopie {Physik} WCE 000: Photobiologie {Biophysik}

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Functional Genetic Analysis Reveals Intricate Roles of Conserved X-box Elements in Yeast Transcriptional Regulation

Ultraschnelle, lichtinduzierte Primärprozesse im elektronisch angeregten Zustand des Grün Fluoreszierenden Proteins (GFP) / Ultrafast Elementary Events in the Excited State of Green Fluorescent Protein (GFP)