Tsim Ting Yuk. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2000. / Includes bibliographical references (leaves 119-135). / Abstracts in English and Chinese. / ABSTRACT --- p.i / 摘要 --- p.iii / 英漢譯名對照 --- p.v / ABBREVIATIONS --- p.vi / ACKNOWLEDGMENTS --- p.viii / Chapter 1. --- Introduction / Chapter 1.1. --- Olfactory system --- p.1 / Chapter 1.1.1. --- Olfactory bulb (OB) --- p.1 / Chapter 1.1.2. --- Accessory olfactory bulb (AOB) --- p.3 / Chapter 1.2. --- Stem cells --- p.5 / Chapter 1.3. --- Sexual differentiation --- p.8 / Chapter 1.3.1. --- Sexual dimorphic olfactory system --- p.8 / Chapter 1.3.2. --- Androgen receptor (AR) & estrogen receptor beta (ERβ) --- p.13 / Chapter 1.3.3. --- Aromatase --- p.15 / Chapter 1.3.4. --- Oligomycin sensitivity-conferringrotein (OSCP) --- p.18 / Chapter 1.4. --- rogrammed cell death (PCD) --- p.18 / Chapter 1.4.1. --- CD in the olfactory development --- p.18 / Chapter 1.4.2. --- Caspase 3 --- p.22 / Chapter 1.4.3. --- B cell leukemia/ Lymphoma 2 (Bcl-2) --- p.23 / Chapter 1.5. --- Axon guidance molecules --- p.25 / Chapter 1.5.1. --- Growth cone --- p.25 / Chapter 1.5.2. --- Mechanisms of growth cone advance --- p.26 / Chapter 1.5.3. --- Semaphorins --- p.28 / Chapter 1.5.4. --- Neuropilin --- p.31 / Chapter 1.5.5. --- lexin --- p.32 / Chapter 1.5.6. --- Collapsin response mediatorroteins (CRMPs) --- p.32 / Chapter 1.6. --- Olfactory markerroteins --- p.33 / Chapter 1.6.1. --- Markerroteins in ORNs --- p.33 / Chapter 1.6.2. --- Growth associatedrotein (GAP-43) --- p.34 / Chapter 1.6.3. --- Is the expression of GAP-43 in rat OB sexually dimorphic? --- p.36 / Chapter 1.6.4. --- Olfactory markerrotein (OMP) --- p.38 / Chapter 1.6.5. --- Golf --- p.39 / Chapter 1.7. --- Miscellaneous genes --- p.40 / Chapter 1.7.1. --- Substance (SP) --- p.40 / Chapter 1.7.2. --- Gonadotropin releasing hormone (GnRH) --- p.41 / Chapter 1.7.3. --- Metabotropic glutamate receptor 2 (mGluR2) --- p.42 / Chapter 1.7.4. --- Insulin-like growth factor binding protein-2 (IGFBP2) --- p.43 / Chapter 2. --- Materials and methods / Chapter 2.1. --- Animal study --- p.46 / Chapter 2.2. --- RNA extraction --- p.46 / Chapter 2.3. --- Quantitation of total RNA --- p.49 / Chapter 2.4. --- Reverse Transcription (RT) --- p.50 / Chapter 2.5. --- olymerase Chain Reaction (PCR) --- p.51 / Chapter 2.6. --- urification ofCRroducts --- p.55 / Chapter 2.7. --- Confirmation ofCRroducts --- p.56 / Chapter 2.8. --- Quantitation of cDNA --- p.57 / Chapter 2.9. --- Radioactive labeledCR --- p.58 / Chapter 2.10. --- Electrophoresis ofCRroducts --- p.59 / Chapter 2.11. --- Statistical analysis --- p.60 / Chapter 3. --- Results / Chapter 3.1. --- Standard curve construction --- p.61 / Chapter 3.2. --- β-actin --- p.62 / Chapter 3.3. --- Sexual differentiation related genes --- p.64 / Chapter 3.3.1. --- AR --- p.64 / Chapter 3.3.2. --- ERβ --- p.65 / Chapter 3.3.3. --- Aromatase --- p.65 / Chapter 3.3.4. --- OSCP --- p.66 / Chapter 3.4. --- CD related genes --- p.66 / Chapter 3.4.1. --- Bcl-2α --- p.66 / Chapter 3.4.2. --- Caspase 3 --- p.67 / Chapter 3.5. --- Axon guidance molecules and related genes --- p.67 / Chapter 3.5.1. --- SemaIII --- p.67 / Chapter 3.5.2. --- Neuropilin-1 --- p.68 / Chapter 3.5.3. --- lexin-1 --- p.68 / Chapter 3.5.4. --- CRMP-1 --- p.69 / Chapter 3.5.5. --- CRMP-2 --- p.70 / Chapter 3.5.6. --- CRMP-3 --- p.70 / Chapter 3.5.7. --- CRMP-4 --- p.71 / Chapter 3.6. --- Olfactory markerrotein genes --- p.71 / Chapter 3.6.1. --- GAP-43 --- p.71 / Chapter 3.6.2. --- OMP --- p.72 / Chapter 3.6.3. --- Golf --- p.72 / Chapter 3.7. --- Miscellaneous genes --- p.73 / Chapter 3.7.1. --- SubstanceP --- p.73 / Chapter 3.7.2. --- GnRH --- p.73 / Chapter 3.7.3. --- mGluR2 --- p.74 / Chapter 3.7.4. --- IGFBP-2 --- p.74 / Chapter 3.8. --- Graphs and tables --- p.75 / Chapter 4. --- Discussion / Chapter 4.1. --- Quantitation of cDNA and normalization of CR results --- p.97 / Chapter 4.2. --- Sexual differentiation related genes --- p.98 / Chapter 4.3. --- CD related genes --- p.100 / Chapter 4.4. --- Axon guidance molecule and related genes --- p.103 / Chapter 4.5. --- Olfactory markerrotein genes --- p.109 / Chapter 4.6. --- Miscellaneous genes --- p.112 / Chapter 5. --- References --- p.119

by Woo Yiu Ho, Anthony. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1998. / Includes bibliographical references (leaves 162-171). / Abstract also in Chinese. / Abstract --- p.i / Acknowledgements --- p.iii / Table of Contents --- p.iv / List of Figures --- p.ix / List of Tables --- p.xi / List of Abbreviations --- p.xii / Chapter 1 --- INTRODUCTION --- p.1 / Chapter 1.1 --- Bacterial Adhesion and Aggregation --- p.1 / Chapter 1.1.1 --- Significance of Bacterial Adhesion Studies --- p.1 / Chapter 1.1.2 --- Definitions --- p.4 / Chapter 1.1.3 --- Colonization Process --- p.7 / Chapter 1.1.4 --- Specific and Nonspecific Interactions --- p.8 / Chapter 1.1.5 --- Models of Bacterial Adhesion and Aggregation Processes --- p.14 / Chapter 1.1.6 --- Experimental Systems in Adhesion Research --- p.16 / Chapter 1.1.7 --- Experimental Systems in Aggregation Research --- p.19 / Chapter 1.2 --- Pseudomonas aeruginosa --- p.21 / Chapter 1.2.1 --- General Description and Clinical Significance --- p.21 / Chapter 1.2.2 --- Adhesins of Pseudomonas aeruginosa --- p.22 / Chapter 1.2.3 --- "Alginate, Mucoidity, Biofilm Formation and Cystic Fibrosis" --- p.23 / Chapter 1.2.4 --- Lipopolysaccharides --- p.26 / Chapter 1.2.5 --- Pili --- p.29 / Chapter 1.2.6 --- Flagella --- p.30 / Chapter 1.2.7 --- Lectins --- p.31 / Chapter 1.2.8 --- Other Adhesins --- p.31 / Chapter 1.2.9 --- Rhamnolipids --- p.32 / Chapter 1.3 --- Current Study --- p.33 / Chapter 2 --- MATERIALS AND EQUIPMENT --- p.35 / Chapter 2.1 --- Bacterial Strain --- p.35 / Chapter 2.2 --- Solid Surfaces --- p.35 / Chapter 2.3 --- Chemicals --- p.36 / Chapter 2.4 --- Recipes --- p.38 / Chapter 2.5 --- Equipment --- p.38 / Chapter 3 --- METHODS --- p.40 / Chapter 3.1 --- Maintenance and Culturation --- p.40 / Chapter 3.1.1 --- Maintenance of Bacterial Strains --- p.40 / Chapter 3.1.2 --- Seed Culture Preparation --- p.40 / Chapter 3.1.3 --- Culturation in Defined Growth Media --- p.40 / Chapter 3.2 --- Bacterial Adhesion and Aggregation Assay Methods --- p.41 / Chapter 3.2.1 --- Bacterial Adhesion on Glass Assay --- p.41 / Chapter 3.2.2 --- Bacterial Adhesion on Plastic Assay --- p.44 / Chapter 3.2.3 --- Bacterial Adhesion under Shear Assay --- p.44 / Chapter 3.2.4 --- Bacterial Aggregation Examination by Adhesion on Glass Assay --- p.45 / Chapter 3.2.5 --- Bacterial Aggregation Examination by Top-agar Assay --- p.45 / Chapter 3.2.6 --- Bacterial Aggregation Examination by Epi-fluorescence Microscopy --- p.46 / Chapter 3.2.7 --- Bacterial Aggregation Screening Test --- p.46 / Chapter 3.3 --- Determination of the Effects of Various Factors on Adhesion and Aggregation --- p.47 / Chapter 3.3.1 --- Culturation Period --- p.47 / Chapter 3.3.2 --- Osmotic Shock during the Washing Procedure --- p.47 / Chapter 3.3.3 --- Growth Media --- p.48 / Chapter 3.3.4 --- Assay Conditions --- p.48 / Chapter 3.3.5 --- Cell Pretreatments --- p.48 / Chapter 3.4 --- Isolation of Aggregation-deficient Mutants --- p.49 / Chapter 3.5 --- Outer Membrane Protein Profiles --- p.50 / Chapter 3.5.1 --- Isolation of Outer Membrane Fraction --- p.50 / Chapter 3.5.2 --- Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis --- p.51 / Chapter 3.6 --- Determination of the Mobility of the Bacteria on Surfaces --- p.52 / Chapter 3.6.1 --- Subsurface Twitching Assay --- p.52 / Chapter 3.6.2 --- Soft-agar Swarm Assay --- p.53 / Chapter 3.7 --- Detection of Alginate Production --- p.53 / Chapter 3.7.1 --- Extraction of Alginate from Spent Growth Medium --- p.53 / Chapter 3.7.2 --- Releasing Cell Surface-associated Alginate --- p.54 / Chapter 3.8 --- Other Assay Methods --- p.55 / Chapter 3.8.1 --- Protein Assay --- p.55 / Chapter 3.8.2 --- Carbohydrate Determination --- p.55 / Chapter 3.8.3 --- Alginate Determination --- p.55 / Chapter 4 --- RESULTS --- p.57 / Chapter 4.1 --- Standardization of the Assays for Bacterial Adhesion and Aggregation --- p.57 / Chapter 4.1.1 --- Effects of Cell Density and Exposure Time on the Number of Adhered Bacteria Detected in Bacterial Adhesion on Glass Assay --- p.57 / Chapter 4.1.2 --- Characterization of Bacterial Aggregation by Different Examination Methods --- p.62 / Chapter 4.1.3 --- Effects of Culturation Period on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.67 / Chapter 4.1.4 --- Effects of Osmotic Shock during Washing on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.70 / Chapter 4.1.5 --- Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 as a Function of Time under the Standard Assay Condition --- p.71 / Chapter 4.1.6 --- Consistency of Bacterial Adhesion on Glass Assay --- p.74 / Chapter 4.2 --- Effects of Growth Media on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.77 / Chapter 4.3 --- Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 in Different Assay Media --- p.77 / Chapter 4.3.1 --- Effects of Various Buffers on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.77 / Chapter 4.3.2 --- Effects of pH on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.78 / Chapter 4.3.3 --- Effects of Various Electrolytes on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.81 / Chapter 4.3.4 --- Concentration Effects of Monovalent and Divalent Cations on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.88 / Chapter 4.3.5 --- Concentration Effects of Phosphate Buffers on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.93 / Chapter 4.3.6 --- Concentration Effects of Ammonium Sulfate and Cyclohexylammonium Sulfate on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.96 / Chapter 4.3.7 --- Effects of Cation Chelation on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.99 / Chapter 4.3.8 --- Effects of Sugars on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.100 / Chapter 4.3.9 --- Effects of Amino Acids on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.101 / Chapter 4.4 --- Adhesion and Aggregation after Pretreatments of the Cells --- p.103 / Chapter 4.4.1 --- Effects of Protease Treatments on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.103 / Chapter 4.4.2 --- Effects of Externally Added Proteins on Adhesion and Aggregation of Pronase-treated Cells --- p.107 / Chapter 4.4.3 --- Effects of Acid or Base Treatments on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.108 / Chapter 4.4.4 --- Effects of Heat Treatment on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.108 / Chapter 4.4.5 --- Effects of Extensive Washing on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.110 / Chapter 4.5 --- Isolation and Growth Characteristics of Aggregation-deficient Mutants --- p.111 / Chapter 4.6 --- Comparisons of the Adhesion and Aggregation Characters of Pseudomonas aeruginosa ATCC 10145 and Mutant 9 --- p.115 / Chapter 4.6.1 --- Under Standard Condition --- p.115 / Chapter 4.6.2 --- On Different Surfaces and in Different Electrolytes --- p.115 / Chapter 4.6.3 --- Under Shear --- p.118 / Chapter 4.6.4 --- Adhesion and Aggregation of Combined Suspensions of Pseudomonas aeruginosa ATCC 10145 and Mutant 9 --- p.122 / Chapter 4.7 --- Characterization of the Cell Surface Properties of Pseudomonas aeruginosa ATCC 10145 and Mutant 9 --- p.125 / Chapter 4.7.1 --- Outer Membrane Protein Profiles --- p.125 / Chapter 4.7.2 --- Pili-elicited Twitching Mobility --- p.125 / Chapter 4.7.3 --- Mobility Due to Flagella --- p.128 / Chapter 4.7.4 --- Production of Alginate --- p.128 / Chapter 5 --- DISCUSSIONS --- p.130 / Chapter 5.1 --- Choice of the Materials --- p.130 / Chapter 5.2 --- Development of the Assay Methods --- p.130 / Chapter 5.2.1 --- Development of the Procedures for Bacterial Adhesion Assays --- p.130 / Chapter 5.2.2 --- Development of the Assay Methods for Bacterial Aggregation --- p.132 / Chapter 5.2.3 --- Standardization of the Assays --- p.133 / Chapter 5.2.4 --- Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 as a Function of Time under the Standard Assay Condition --- p.134 / Chapter 5.2.5 --- Consistency of Bacterial Adhesion on Glass Assay --- p.135 / Chapter 5.2.6 --- Limits of Bacterial Adhesion on Glass Assay --- p.135 / Chapter 5.3 --- Effects of Growth Media on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.135 / Chapter 5.4 --- Effects of Various Chemicals in the Assay Media on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.136 / Chapter 5.4.1 --- Effects of Electrolytes on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.137 / Chapter 5.4.2 --- Effects of Aggregation on Adhesion --- p.140 / Chapter 5.4.3 --- Effects of Cyclohexylammonium Sulfate and Ammonium Sulfate on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.141 / Chapter 5.4.4 --- Effects of Sugars and Amino Acids on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.143 / Chapter 5.5 --- Effects of Various Cell-surface Modifications on Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 --- p.144 / Chapter 5.6 --- Isolation and Growth Characteristics of Aggregation-deficient Mutants --- p.146 / Chapter 5.7 --- Comparisons of the Adhesion and Aggregation Characters of Pseudonomas aeruginosa ATCC 10145 and Mutant 9 --- p.147 / Chapter 5.7.1 --- Adhesion and Aggregation of Pseudonomas aeruginosa ATCC 10145 and Mutant 9 on Different Surfaces In Different Electrolytes --- p.147 / Chapter 5.7.2 --- Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 and Mutant 9 Under Shear --- p.147 / Chapter 5.7.3 --- Adhesion and Aggregation of Combined Suspensions of Pseudomonas aeruginosa ATCC 10145 and Mutant 9 --- p.148 / Chapter 5.8 --- Characterization of the Cell Surface Properties of Pseudomonas aeruginosa ATCC 10145 and Mutant 9 --- p.148 / Chapter 5.9 --- General Discussions --- p.151 / Chapter 6 --- APPENDIX --- p.154 / Chapter 6.1 --- Visual Examination of Adhesion and Aggregation of Pseudomonas aeruginosa ATCC 10145 on Glass --- p.154 / Chapter 6.2 --- Fractal Analysis of Bacterial Aggregates --- p.154 / Chapter 7 --- REFERENCES --- p.162

No description available.

Tjia Wai Mui. / Thesis submitted in: July 2002. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2003. / Includes bibliographical references (leaves 123-140). / Abstracts in English and Chinese. / Abstract (English) --- p.i / Abstract (Chinese) --- p.iii / Acknowledgement --- p.iv / Abbreviations --- p.v / Table of Contents --- p.vi / List of Figures --- p.x / List of Tables --- p.xii / Chapter Chapter One --- Literature Review / Chapter 1.1 --- Introduction --- p.1 / Chapter 1.2 --- Growth of L. edodes --- p.3 / Chapter 1.2.1 --- Life cycle of L. edodes --- p.3 / Chapter 1.2.2 --- Growth parameters of L. edodes --- p.6 / Chapter 1.2.2.1 --- Temperature --- p.6 / Chapter 1.2.2.2 --- Relative humidity --- p.7 / Chapter 1.2.2.3 --- Moisture content in substrate --- p.7 / Chapter 1.2.2.4 --- Light --- p.8 / Chapter 1.2.2.5 --- pH --- p.8 / Chapter 1.3 --- Cultivation of L. edodes --- p.9 / Chapter 1.3.1 --- History and development of artificial cultivation --- p.9 / Chapter 1.3.2 --- Use of forced fruiting --- p.11 / Chapter 1.4 --- Molecular studies of stress on fungi --- p.12 / Chapter 1.4.1 --- Studies of temperature stress in mushroom --- p.12 / Chapter 1.4.2 --- Studies of molecular chaperones in fungi --- p.13 / Chapter 1.4.2.1 --- Role of molecular chaperones --- p.13 / Chapter 1.4.2.2 --- Heat shock protein 70 (Hsp70) and their cochaperones --- p.13 / Chapter 1.4.2.3 --- Other chaperones --- p.15 / Chapter 1.4.2.4 --- Molecular chaperones and development --- p.16 / Chapter 1.5 --- Prospectus --- p.19 / Chapter Chapter Two --- The Effect of Stress on the Growth of L. edodes / Chapter 2.1 --- Introduction --- p.23 / Chapter 2.2 --- Materials and Methods --- p.24 / Chapter 2.2.1 --- Strain and culture conditions --- p.24 / Chapter 2.2.2 --- Stress treatments --- p.24 / Chapter 2.2.3 --- Data collection --- p.25 / Chapter 2.2.4 --- Data analysis --- p.25 / Chapter 2.3 --- Results --- p.27 / Chapter 2.3.1 --- Reliability analysis --- p.27 / Chapter 2.3.2 --- Descriptive statistics --- p.28 / Chapter 2.3.3 --- Independent t-test (ANOVA) --- p.33 / Chapter 2.4 --- Discussion --- p.37 / Chapter Chapter Three --- Sequence Analysis of selected Stress Genes / Chapter 3.1 --- Introduction --- p.39 / Chapter 3.2 --- Materials and Methods --- p.40 / Chapter 3.2.1 --- Isolation of stress genes --- p.40 / Chapter 3.2.1.1 --- Construction of primordial cDNA library --- p.40 / Chapter 3.2.1.2 --- Screening of cDNA clones --- p.40 / Chapter 3.2.2 --- Sequence analyses of stress genes --- p.41 / Chapter 3.2.2.1 --- Amplification and purification of cDNA insert --- p.41 / Chapter 3.2.2.2 --- Full length DNA cycle sequencing --- p.42 / Chapter 3.2.2.3 --- Sequence analyses --- p.43 / Chapter 3.2.3 --- Screening of LeSSA (Inducible HSP70) --- p.45 / Chapter 3.2.3.1 --- PCR screening of LeSSA by degenerate primers and LeSSB specific primers --- p.45 / Chapter 3.2.3.2 --- Screening of LeSSA from cDNA library by hybridization --- p.49 / Chapter 3.3 --- Results --- p.51 / Chapter 3.3.1 --- Sequence analyses --- p.51 / Chapter 3.3.1.1 --- LeSSB --- p.51 / Chapter 3.3.1.2 --- LeMge1 --- p.57 / Chapter 3.3.1.3 --- LeSTI1 --- p.62 / Chapter 3.3.1.4 --- LeTCP1β --- p.69 / Chapter 3.3.1.5 --- LeTCP1γ --- p.74 / Chapter 3.3.2 --- Failure of isolating LeSSA (Inducible HSP70) --- p.80 / Chapter 3.4 --- Discussion --- p.82 / Chapter 3.4.1 --- Sequence analyses --- p.82 / Chapter 3.4.2 --- Screening of LeSSA --- p.84 / Chapter Chapter Four --- Characterization of stress genes upon different stresses / Chapter 4.1 --- Introduction --- p.86 / Chapter 4.2 --- Materials and Methods --- p.87 / Chapter 4.2.1 --- Strain and culture conditions --- p.87 / Chapter 4.2.2 --- Stress treatments --- p.87 / Chapter 4.2.3 --- Isolation of total RNAs --- p.87 / Chapter 4.2.4 --- Reverse transcriptase-polymerase chain reaction (RT-PCR) --- p.88 / Chapter 4.2.4.1 --- Reverse transcription --- p.88 / Chapter 4.2.4.2 --- PCR amplification by specific primers of stress genes --- p.89 / Chapter 4.2.5 --- Northern blot analyses --- p.91 / Chapter 4.2.5.1 --- RNA fractionation by formaldehyde gel electrophoresis --- p.91 / Chapter 4.2.5.2 --- Northern blotting --- p.91 / Chapter 4.2.5.3 --- Preparation of probes --- p.92 / Chapter 4.2.5.4 --- Hybridization and stringency washes --- p.93 / Chapter 4.2.6 --- Isolation of total protein --- p.94 / Chapter 4.2.7 --- Quantification of protein by Bradford method --- p.95 / Chapter 4.2.8 --- Western blot analyses --- p.95 / Chapter 4.2.8.1 --- Sodium dodecyl sulfate ´ؤ polyacrylamide gel electrophoresis (SDS-PAGE) --- p.95 / Chapter 4.2.8.2 --- Western blotting --- p.96 / Chapter 4.2.8.3 --- Immunodetection --- p.98 / Chapter 4.2.8.4 --- ECL detection --- p.98 / Chapter 4.3 --- Results --- p.99 / Chapter 4.3.1 --- Reverse transcriptase-polymerase chain reaction (RT-PCR) --- p.99 / Chapter 4.3.2 --- Northern blot hybridization --- p.106 / Chapter 4.3.2.1 --- Establishing an internal control --- p.106 / Chapter 4.3.2.2 --- Dig-labelling of stress genes --- p.106 / Chapter 4.3.2.3 --- Northern blot hybridizaton of stress genes --- p.106 / Chapter 4.3.3 --- Western blot hybridization --- p.111 / Chapter 4.4 --- Discussions --- p.113 / Chapter Chapter Five --- General Discussions --- p.118 / References --- p.123

Kong Fung Wong. / Thesis (M.Phil.)--Chinese University of Hong Kong, 1997. / Includes bibliographical references (leaves 59-70). / Chapter CHATPER 1 --- GENERAL INTRODUCTION --- p.1-12 / Chapter CHATPER 2 --- EXAMINATION OF THE BEHAVIOR OF GROWTH CONE IN DIFFERENT REGIONS OF THE OPTIC CHIASM / Introduction --- p.13-14 / Materials and Methods --- p.15-18 / Results --- p.19-23 / Discussion --- p.24-27 / Chapter CHATPER 3 --- STUDY OF BINOCULAR INTERACTION AFTER UNILATERAL INTRA-UTERO ENUCLEATION / Introduction --- p.28-29 / Materials and Methods --- p.30-31 / Results --- p.32-35 / Discussion --- p.36-39 / Chapter CHATPER 4 --- ISOLATION OF DIFFERENTIALLY EXPRESSED mRNA IN DIFFERENT REGIONS OF THE RETINA / Introduction --- p.40-43 / Materials and Methods --- p.44-48 / Results --- p.48-50 / Discussion --- p.51-54 / Chapter CHATPER 5 --- GENERAL DISCUSSION --- p.56-58 / REFERENCE --- p.59-70 / FIGURES / TABLES

The Total Sanitation Campaign is an initiative launched by the Government of India in 1999 to accelerate sanitation coverage throughout the country. This thesis measures the impact of the Total Sanitation Campaign on education in Indian government schools. I assess whether access to toilets, access to water or access to both toilets and water impact the following parameters of education: literacy, current enrollment in school or completed years of education. Data is sourced from the Indian Human Development Survey (IHDS) 2005, sorted for the nineteen major states in India and aggregated at a district level for each state. The analysis employs two separate probit regression models to assess sanitation facilities’ impact on literacy and current enrollment in school, and a robust linear model to assess sanitation facilities’ impact on completed years of education. The models control for age, sex, caste, religion, household location, household size and household income. The results indicate that sanitation facilities positively impact education based on the age, sex and caste of the sample population. These findings present implications for future policymaking in order to improve access to and participation in education.

Thesis (M.Dev)--University of Limpopo, 2007. / This study is the first of its kind in the Limpopo Province, consequently, much of its literature focus on Strategic Planning in general. The researcher was motivated to undertake this study due to the manner in which departments were producing Strategic Plans. Different formats were used in departments, Strategic Planning was treated as an add-on activity to personnel, and there is also the lack of alignment and integration between the Strategic Plans and PGDS. The objective of the study is to asses the existing capacity of departments to perform Strategic Planning functions that will inform growth and development in the province; to asses the level of compliance with the Medium Term Strategic Framework; and to recommend strategies to enhance Strategic Planning capacity in departments, thereby ensuring alignment with PGDS. This study, thus examines the capacity of all departments, including the Office of the Premier, to implement the objectives of the PGDS, with specific focus on the Strategic Plans, the type and number of human resources available to perform the duties of Strategic Planning. The researcher follows a qualitative approach. Questionnaires, which comprised both closed and open–ended questions, were e-mailed to 11 provincial department in Limpopo Province and the target respondents were planners, Chief Financial Officers and workstudy officers. Discussions and interviews were conducted with Heads of Departments and Planners, respectively. The results from the SPSS reconciled with the qualitative analysis on open-ended questions show that there is limited planning capacity in the majority of provincial departments in the Limpopo Province. Further research is required with regard to project-specific strategic planning that supports the achievement of the PGDS. / Office of the Premier (Limpopo Government)

This dissertation is composed of three essays under the recurring theme of women’s issues, child health and economic development. In the first chapter, I examine the effect of Child Marriage on the health outcomes of offspring in Bangladesh. I use an exogenous variation in drought and flood shocks as an instrument for Child Marriage and measure children’s health outcomes by stunting, from the height-for-age index. I develop a theoretical model to show that the decision to marry a daughter early is driven by the type of income shocks that a household is exposed to. From the empirical estimation, I find that children from Child Marriage unions are more likely to be stunted. I also find that the effect of Child Marriage is concentrated through increases in severe stunting. In the second chapter, I estimate the impact of Child Marriage on the mortality outcomes of offspring in Bangladesh. Similar to the empirical strategy in the first chapter, I focus on the effect of an exogenous temporary shock on the marriage decision, to allow for identification of a causal impact. The results indicate that in comparison to children of later-married women, children from Child Marriage unions are more likely to die before reaching the age of five. Further, I find that the effect of Child Marriage is smaller on infant mortality. By examining the impact by gender, the results suggest that the predominant effect of Child Marriage is focused on increases in male child and infant mortality. In the third chapter, I examine the effect of Sharia Law on the health outcomes of children in Nigeria. The measures of health outcomes are constructed from the height-for-age, weight-for-age and weight-for-height indices. I apply a difference-in-differences estimation strategy and exploit the variation in exposure to Sharia law across time and state of residence. I also explore the effect of Sharia Law on women’s empowerment. The results indicate that Sharia Law substantially increases the probability of stunting in children. In states with high enforcement of Sharia Law, children are more likely to be stunted and underweight. The results suggest a negative association between Sharia Law and women’s empowerment with respect to household and medical decision-making, control over resources and self-worth.

Thesis (D. Min.)--Western Theological Seminary, 1987. / Includes abstract. Includes bibliographical references (leaves 430-432).

No description available.