Real-world radiance values can range over eight orders of magnitude from starlight to direct sunlight but few digital cameras capture more than three orders in a single Low Dynamic Range (LDR) image. We approach this problem using established High Dynamic Range (HDR) techniques in which multiple images are captured with different exposure times so that all portions of the scene are correctly exposed at least once. These images are then combined to create an HDR image capturing the full range of the scene. HDR capture introduces new challenges; movement in the scene creates faded copies of moving objects, referred to as ghosts. Many techniques have been introduced to handle ghosting, but typically they either address specific types of ghosting, or are computationally very expensive. We address ghosting by first detecting moving objects, then reducing their contribution to the final composite on a frame-by-frame basis. The detection of motion is addressed by performing change detection on exposure-normalized images. Additional special cases are developed based on a priori knowledge of the changing exposures; for example, if exposure is increasing every shot, then any decrease in intensity in the LDR images is a strong indicator of motion. Recent Superpixel over-segmentation techniques are used to refine the detection. We also propose a novel solution for areas that see motion throughout the capture, such as foliage blowing in the wind. Such areas are detected as always moving, and are replaced with information from a single input image, and the replacement of corrupted regions can be tailored to the scenario. We present our approach in the context of a panoramic tele-presence system. Tele-presence systems allow a user to experience a remote environment, aiming to create a realistic sense of "being there" and such a system should therefore provide a high quality visual rendition of the environment. Furthermore, panoramas, by virtue of capturing a greater proportion of a real-world scene, are often exposed to a greater dynamic range than standard photographs. Both facets of this system therefore stand to benefit from HDR imaging techniques. We demonstrate the success of our approach on multiple challenging ghosting scenarios, and compare our results with state-of-the-art methods previously proposed. We also demonstrate computational savings over these methods.

Real-world radiance values can range over eight orders of magnitude from starlight to direct sunlight but few digital cameras capture more than three orders in a single Low Dynamic Range (LDR) image. We approach this problem using established High Dynamic Range (HDR) techniques in which multiple images are captured with different exposure times so that all portions of the scene are correctly exposed at least once. These images are then combined to create an HDR image capturing the full range of the scene. HDR capture introduces new challenges; movement in the scene creates faded copies of moving objects, referred to as ghosts. Many techniques have been introduced to handle ghosting, but typically they either address specific types of ghosting, or are computationally very expensive. We address ghosting by first detecting moving objects, then reducing their contribution to the final composite on a frame-by-frame basis. The detection of motion is addressed by performing change detection on exposure-normalized images. Additional special cases are developed based on a priori knowledge of the changing exposures; for example, if exposure is increasing every shot, then any decrease in intensity in the LDR images is a strong indicator of motion. Recent Superpixel over-segmentation techniques are used to refine the detection. We also propose a novel solution for areas that see motion throughout the capture, such as foliage blowing in the wind. Such areas are detected as always moving, and are replaced with information from a single input image, and the replacement of corrupted regions can be tailored to the scenario. We present our approach in the context of a panoramic tele-presence system. Tele-presence systems allow a user to experience a remote environment, aiming to create a realistic sense of "being there" and such a system should therefore provide a high quality visual rendition of the environment. Furthermore, panoramas, by virtue of capturing a greater proportion of a real-world scene, are often exposed to a greater dynamic range than standard photographs. Both facets of this system therefore stand to benefit from HDR imaging techniques. We demonstrate the success of our approach on multiple challenging ghosting scenarios, and compare our results with state-of-the-art methods previously proposed. We also demonstrate computational savings over these methods.

Real-world radiance values can range over eight orders of magnitude from starlight to direct sunlight but few digital cameras capture more than three orders in a single Low Dynamic Range (LDR) image. We approach this problem using established High Dynamic Range (HDR) techniques in which multiple images are captured with different exposure times so that all portions of the scene are correctly exposed at least once. These images are then combined to create an HDR image capturing the full range of the scene. HDR capture introduces new challenges; movement in the scene creates faded copies of moving objects, referred to as ghosts. Many techniques have been introduced to handle ghosting, but typically they either address specific types of ghosting, or are computationally very expensive. We address ghosting by first detecting moving objects, then reducing their contribution to the final composite on a frame-by-frame basis. The detection of motion is addressed by performing change detection on exposure-normalized images. Additional special cases are developed based on a priori knowledge of the changing exposures; for example, if exposure is increasing every shot, then any decrease in intensity in the LDR images is a strong indicator of motion. Recent Superpixel over-segmentation techniques are used to refine the detection. We also propose a novel solution for areas that see motion throughout the capture, such as foliage blowing in the wind. Such areas are detected as always moving, and are replaced with information from a single input image, and the replacement of corrupted regions can be tailored to the scenario. We present our approach in the context of a panoramic tele-presence system. Tele-presence systems allow a user to experience a remote environment, aiming to create a realistic sense of "being there" and such a system should therefore provide a high quality visual rendition of the environment. Furthermore, panoramas, by virtue of capturing a greater proportion of a real-world scene, are often exposed to a greater dynamic range than standard photographs. Both facets of this system therefore stand to benefit from HDR imaging techniques. We demonstrate the success of our approach on multiple challenging ghosting scenarios, and compare our results with state-of-the-art methods previously proposed. We also demonstrate computational savings over these methods.

L'un des buts principaux de l'imagerie numérique est d'une part la capture et d'autre part la reproduction de scènes réelles ou synthétiques sur des dispositifs d'affichage aux capacités restreintes. Les techniques d'imagerie traditionnelles sont limitées par la gamme de luminance qu'elles peuvent capturer et afficher. L'imagerie à grande gamme de luminance (High Dynamic Range – HDR) vise à dépasser cette limitation en capturant, représentant et affichant les quantités physique de la lumière présente dans une scène. Cependant, les technologies d'affichage existantes ne vont pas disparaitre instantanément, la compatibilité entre ces nouveaux contenus HDR et les contenus classiques est donc requise. Cette compatibilité est assurée par une opération de réduction des gammes de luminance (tone mapping) qui adapte les contenus HDR aux capacités restreintes des écrans. Bien que de nombreux opérateurs de tone mapping existent, ceux-ci se focalisent principalement sur les images fixes. Les verrous scientifiques associés au tone mapping de vidéo HDR sont plus complexes du fait de la dimension temporelle. Les travaux recherche menés dans la thèse se sont focalisés sur la préservation de la cohérence temporelle du vidéo tone mapping. Deux principaux axes de recherche ont été traités : la qualité subjective de contenus tone mappés et l'efficacité de la compression des vidéos HDR. En effet, tone mapper individuellement chaque image d'une séquence vidéo HDR engendre des artefacts temporels. Ces artefacts affectent la qualité visuelle de la vidéo tone mappée et il est donc nécessaire de les minimiser. Au travers de tests effectués sur des vidéos HDR avec différents opérateurs de tone mapping, nous avons proposé une classification des artefacts temporels en six catégories. Après avoir testé les opérateurs de tone mapping vidéo existants sur les différents types d'artefacts temporels, nous avons observé que seulement trois des six types d'artefacts étaient résolus. Nous avons donc créé une technique de post-traitement qui permet de réduire les 3 types d'artefacts non-considérés. Le deuxième aspect considéré dans la thèse concerne les relations entre compression et tone mapping. Jusque là, les travaux effectués sur le tone mapping et la vidéo compression se focalisaient sur l'optimisation du tone mapping de manière à atteindre des taux de compression élevés. Ces techniques modifient fortement le rendu, c'est à dire l'aspect de la vidéo, modifiant ainsi l'intention artistique initiale en amont dans la chaine de distribution (avant la compression). Dans ce contexte, nous avons proposé une technique qui permet de réduire l'entropie d'une vidéo tone mappée sans en modifier son rendu. Notre méthode adapte la quantification afin d'accroitre les corrélations entre images successives d'une vidéo. / One of the main goals of digital imagery is to improve the capture and the reproduction of real or synthetic scenes on display devices with restricted capabilities. Standard imagery techniques are limited with respect to the dynamic range that they can capture and reproduce. High Dynamic Range (HDR) imagery aims at overcoming these limitations by capturing, representing and displaying the physical value of light measured in a scene. However, current commercial displays will not vanish instantly hence backward compatibility between HDR content and those displays is required. This compatibility is ensured through an operation called tone mapping that retargets the dynamic range of HDR content to the restricted dynamic range of a display device. Although many tone mapping operators exist, they focus mostly on still images. The challenges of tone mapping HDR videos are more complex than those of still images since the temporal dimensions is added. In this work, the focus was on the preservation of temporal coherency when performing video tone mapping. Two main research avenues are investigated: the subjective quality of tone mapped video content and their compression efficiency. Indeed, tone mapping independently each frame of a video sequence leads to temporal artifacts. Those artifacts impair the visual quality of the tone mapped video sequence and need to be reduced. Through experimentations with HDR videos and Tone Mapping Operators (TMOs), we categorized temporal artifacts into six categories. We tested video tone mapping operators (techniques that take into account more than a single frame) on the different types of temporal artifact and we observed that they could handle only three out of the six types. Consequently, we designed a post-processing technique that adapts to any tone mapping operator and reduces the three types of artifact not dealt with. A subjective evaluation reported that our technique always preserves or increases the subjective quality of tone mapped content for the sequences and TMOs tested. The second topic investigated was the compression of tone mapped video content. So far, work on tone mapping and video compression focused on optimizing a tone map curve to achieve high compression ratio. These techniques changed the rendering of the video to reduce its entropy hence removing any artistic intent or constraint on the final results. That is why, we proposed a technique that reduces the entropy of a tone mapped video without altering its rendering.

This thesis deals with design and realization of methods of preprocessing of X-ray images and its storage. In the first part of this thesis, there were designed and implemented methods for preprocessing of series of X-ray images such as averaging after image registration or merging of images to a HDR image using Debevec method. In the following part of the thesis, there was done a literary research of data formats based on which was implemented a library for x-ray images storage. Both implemented methods allow to reduce a random noise by merging a series of images. Application of the Debevec method also allow to increase a dynamic range of image.

In the field of lighting technology, glare evaluation is one of the basic parameters of determining the quality of lighting systems. Disturbing glare is most often evaluated by UGR in internal systems. The UGR method is well described and verified for uniform glare sources. With the development of LED, non-uniform sources of glare become involved in the lighting system. Thus verification of UGR suitability for non-uniform sources is necessary and possibly modification of the method or replacement it by another more appropriate method is required. This diploma thesis focuses on evaluation methods of disturbing glare from luminaires with non-uniform radiation surface. For this purpose, the thesis includes glare analysis, methods of glare calculation and glare evaluation. Furthermore, the UGR analysis and modification for the calculation of disturbing glare from non-uniform glare sources are included. The practical part is concentrated on measurement of glare from uniform and non-uniform glare sources. For the purpose of evaluating the suitability of the measurement method, the subjective evaluation of glare from a statically significant sample is included in the thesis.

The diploma thesis provides the theoretical background needed to understand the complex topic of high dynamic range, and deals with the implementation of an innovative method for temporal video tone-mapping. At first, high dynamic range image acquisition, storage and processing are described. Afterwards, a general introduction to tone-mapping, description of undesirable image artifacts and an overview of existing state-of-the-art algorithms are presented. The part which follows focuses on the implementation of the chosen method and proposes several additional enhancements of the initially selected algorithm. Based on the information provided, an application with an intuitive graphical interface, which can be used for temporal HDR video tone-mapping, has been created. Finally, the the achieved results are demonstrated and visually compared with existing methods for temporal video tone-mapping.

CRISPR/Cas9 induced DNA breaks can be precisely repaired by cellular homology-directed repair (HDR) pathways using exogenously provided template DNA (donor). However, the full potential of precision editing is hindered in many model systems by low cutting efficiencies, low HDR efficiencies and, cytotoxicity related to Cas9 and donor DNA. In this thesis, I address these challenges and present methods that we developed to increase HDR efficiencies in multiple model organisms. In Caenorhabditis elegans, we show that by reducing toxicity high editing efficiencies can be achieved with single stranded oligonucleotide (ssODN) donors. We demonstrate that melting dsDNA donors dramatically improves the knock-in efficiencies of longer (1kb) edits. In addition, we describe 5′-terminal modifications to the donor molecules that further increase the frequency of precision editing. With our methodology a single optimally injected animal can yield more than 100 Green Fluorescent Protein (GFP) positive progeny, dramatically enhancing efficiency of genome editing. Next, we demonstrate the generality of 5′ modified donors by extending our studies to human cell cultures and mice zygotes. In mammalian models, 2′OMe-RNA modifications consistently increase HDR efficiencies by several fold over unmodified donors. Furthermore, end-modified donors exhibited a striking reduction in end-joining reactions including reduced concatemer formation and reduced direct ligation into the host genome. Our study demonstrates that HDR can be improved without inhibiting competing end-joining pathways and provides a platform to identify new chemical modifications that could further increase the potency and efficacy of precision genome editing.

This Master’s Thesis on Simulation of LED retrofits under operational conditions is divided into theoretical and practical part. Theoretical part in the introductory section deals with the concepts of lighting technology and presents some electric light sources, such as classic bulbs, halogen bulbs, linear and compact fluorescent lamps. It also describes the principle of electroluminescent light sources (LED), which discusses principles of yielding a white light, the main advantages of LED etc. The reader is familiar with the issue of compensation the linear fluorescent lamps tubular LED source and with the work environment programme ReluxPro that is used for design of the lighting system. Practical part of the thesis is divided into two chapters. In the first chapter, measured linear fluorescent lamps and LED modules for the light-technical parameters, luminous intensity, radiation spectrum and settling time of light sources are evaluated and compared. The second chapter is solved by using ReluxPro, which explores the impact on the lighting and the brightness of the room layout SA 5.10 while using various light sources. In conclusion, there is an economic aspect of linear fluorescent lamps and LED modules analyzed. Results from the program ReluxPro are continuously compared with real measured values.

Grayscale pictures were never replaced by color ones and will probably never be, because they are indispensable in many areas. In this thesis was chosen three methods for color-to-gray conversion. These methods was implemented afterwards. Within this thesis was also made test set of 24 HDR pictures, which was together with existing set of 24 LDR pictures used to test and evaluate features of implemented methods. These methods were also compared from the point of accurancy and preference using questionnaire.