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Made available in DSpace on 2014-06-11T19:22:22Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-01-27Bitstream added on 2014-06-13T18:08:08Z : No. of bitstreams: 1 chedid_ra_me_jabo.pdf: 1366238 bytes, checksum: 21a6e3e47bfbdb87f49b7e199bfd5d21 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Leporinus obtusidens apresenta características zootécnicas interessantes e promissoras para o cultivo em piscicultura. Na maioria das espécies cultivadas, as larvas, ao iniciarem a alimentação exógena, são organismos cujo desenvolvimento ainda não se completou, razão pela qual os órgãos digestivos não estão totalmente definidos e o conteúdo enzimático ainda é deficiente. As pesquisas com larvas de peixes apontam para a alimentação como o fator de maior importância a ser considerado durante o desenvolvimento inicial, pois os organismos estão na fase de diferenciação estrutural e funcional do sistema digestório. Assim o objetivo deste trabalho foi analisar as características histológicas e histoquímicas do tudo digestivo de Leporinus obtusidens nas fases iniciais do desenvolvimento. As amostras foram fixadas em solução de Karnovsky e processadas para análises histológicas e histoquímicas. A eclosão dos eleuteroembriões de L. obtusidens ocorreu às 15 horas após a fertilização (28°C) e as reservas de vitelo foram observadas até 120 horas após a eclosão (HAE). A boca sofreu modificações na posição, passando de ventral para subterminal. Com relação ao esôfago, foi possível observar as primeiras células caliciformes com 48 HAE e a partir de 64 HAE intensa positividade ao PAS e ao AB. A partir de 30 HAE foi possível observar o primórdio do estômago, caracterizado pela substituição do epitélio esofágico estratificado por epitélio gástrico simples, com presença de poucas células caliciformes. Com 96 HAE foi observada intensa reação ao PAS no epitélio de revestimento do estômago, o que indica funcionalidade do órgão. Quanto ao intestino, foi observado um desenvolvimento lento, as primeiras células caliciformes foram observadas com 96 HAE. Os cecos pilóricos foram evidenciados... / Leporinus obtusidens husbandry has characteristics very interesting and promising for cultivation in aquaculture programs. Most of the cultivated species, the larvae begin to feed exogenously, are organisms whose metamorphosis is not complete, which is why the digestive organs are not fully defined and the enzyme content is still deficient. The studies with fish larvae feeding point to as the most important factor to be considered during early development, because the organisms are in the process of structural and functional differentiation of the digestive system. Thus the objective of this study was to analyze the histological and histochemical features of digestive L. obtusidens in all stages of development. Samples were collected in the Hydrobiology and Aquaculture Station of Companhia Energetica de São Paulo (CESP) Jupiá, SP. The samples were fixed in Karnovsky solution, processed for histological and immunohistochemical analysis. The outbreak of eleuteroembryo L. obtusidens occurred approximately 15 hours after fertilization (28 ° C) and the yolk reserves were observed up to 120 HAE. The mouth has undergone successive changes during development, the ventral position to the subterminal position. With regard to the esophagus, it was possible to observe the first goblet cells with 48 HAE and and starting at 64 HAE intense positivity to PAS and AB. From 30 HAE was possible to observe the beginnings of the stomach, characterized only by the substitution of an esophageal epithelium stratified by gastric epithelium simple with cuboidal cells, with little presence of goblet cells. Was observed with 96 HAE intense reaction to PAS in the epithelium lining of the stomach, indicating functionality of the organ. As the intestine, we observed a slow development, the first goblet cells were observed... (Complete abstract click electronic access below)

Cistopus sp. is a medium to large octopod and it is common in the fish markets of Taiwan. The diagnostic character of the genus Cistopus is the possession of eight mucous pouches in the web between the base of each arm. However, researches on this special structure were absent. This study is to describe the morphological characters of Cistopus sp. from Taiwanese waters and to examine the mucous pouch histologically. The mitochondrial COI gene sequences are used as a tool to analyze the phylogenetic relationships between Cistopus and other genera of the family Octopodidae. Based on the results of this study, the Cistopus of Taiwanese waters is different from the genuine C. indicus and the Cistopus of the Andaman Sea. Cistopus sp. is widely distributed from coastal Asia to India at least and presents sexual dimorphism in the openings of the mucous pouches. The inner wall of the mucous pouches is composed of columnar epithelial cells, and the mucus is secreted from the epithelial cells. The substance secreted from the inner wall is acid mucopolysaccharides. According to the phylogenetic analysis of the octopods COI DNA sequences, Cistopus is grouped with other members of subfamily Octopodinae to form a monophyletic group.

Inflammatory Airway Disease has been reported in young racehorses worldwide. The aim of this study was to determine the prevalence of airway inflammation in an actively racing population of horses and to describe and quantify the degree of lung lesions in this population of horses. The Ontario Death Registry program provided a unique opportunity to evaluate the lungs of actively racing horses that died or were euthanized due to catastrophic injuries while racing or training. Lung sections of 95 horses were included in the study and evaluated using a previously validated histological airway scoring system. Additionally, staining with Toluidine blue and immunohistochemical labeling was performed on a subset of horses to further characterize the inflammation. Inflammatory cell infiltration, smooth muscle hyperplasia and hemosiderin were commonly found. The airway lesion scores were significantly higher in the caudal and dorsal sections of the lungs than in other areas. There was no correlation between the individual scores and either breed, sex, age, cause of death or performance index. The inflammatory and smooth muscle scores were normally distributed and the hemosiderin score was not. The inflammatory cell infiltration was composed of mononuclear cells, with increased number of mast cells and eosinophils in 3/20 and 12/95 horses respectively. Immunohistochemical labeling showed the inflammation around the airway to be composed of 41.0% CD3-positive T cells and 5.8% CD79a-positive B cells. In addition to the airway findings, inflammatory cell aggregates were observed around the pulmonary blood vessels and in the alveolar septa in 67/95 and 71/95 horses respectively. In conclusion, this study provides a histologic evaluation of a population of actively racing horses in which airway inflammation is a common finding.

Diss. (sammanfattning) Uppsala : Univ., 2004. / Härtill 5 uppsatser.

Thesis (Ph.D.)-- University of Adelaide, Dept. of Pathology, 1981. / Photocopy (Vol. 1).

Thesis (Ed. D.)--University of Washington, 2004. / Vita. Includes bibliographical references (leaves 105-108).

Thesis (MSc.(Anatomy)--Faculty of Health Sciences)-University of Pretoria, 2007. / Includes bibliographical references.

Thesis (MSc.(Anatomy)--Faculty of Health Sciences)-University of Pretoria, 2007. / Includes bibliographical references.