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1	Vliv citrulinace histonových proteinů na genovou expresi vybraných genů u buněk myeloidní řady / Influence of the citrulination of histon proteins on the expression of selected genes in myeloid cells Tučková, Kristýna January 2019 (has links) Neutrophils are major cell type of innate immunity, that can eliminate pathogens by different mechanisms. One of these mechanisms is called NETosis, which leads to release of decondensed chromatin and citrullinated histone proteins. Citrullination is post-translational modification catalysed by peptidylarginine deiminase (PAD) and causing transformation of possitively charged arginin to neutral citrullin and can change expression of cytokine genes. Concetrations of pro-inflammatory cytokines (IL-8, TNF, IL-1) were measured after activation of PAD4 and induction of citrullination. Calcium ionophore was used to induce citrullinaton, Cl-amidine and TDFA were used as inhibitors. Production of cytokines was assessed by ELISA on protein level and by qPCR on mRNA level. It was found that induction of citrullination led to increased concentrations of IL-8 and IL-1. Elevated gene expression of IL-8 was confirmed on mRNA level. Both inhibitors were able to decrease level of histone H3 citrullination and IL-8 and IL-1 concentrations. Expression of TNF was not detected on protein and mRNA level.
2	Vztah mezi sestřihem a posttranslačními modifikacemi chromatinu v Saccharomyces cerevisiae / The relationship between splicing and posttranslational modifications of chromatin in Saccharomyces cerevisiae Kovaľová, Libuša January 2018 (has links) Protein Prp45, the yeast ortholog of the human transcription coregulator SNW1/SKIP, has been previously associated only with the regulation of pre-mRNA splicing. However, our laboratory found that protein Prp45 genetically interacts not only with the proteins involved in pre-mRNA splicing, but also with factors important for transcription elongation and with chromatin modifying enzymes. Our data and the information about the human ortholog SNW1/SKIP suggest that Prp45 could serve as a regulator coupling splicing, transcription and chromatin state in S. cerevisiae. The main aim of this diploma thesis was to find out whether the protein Prp45, which is essential for cotranscriptional assembly of the spliceosome, affects posttranslational modifications of chromatin on transcribed genes. Using chromatin immunoprecipitation, the influence of prp45(1-169) mutation on trimethylation of histone H3 at lysine 4 and acetylation of histone H3 at lysines 9, 14 and 18 on transcriptionally active genes was not confirmed. The other aim was to analyse the behavior of cells synchronized by α-factor by using flow cytometry. According to our results, prp45(1-169) mutation leads to the prolongation of the cell cycle. For the purpose of monitoring the dynamics of nucleosomes in S. cerevisiae strains, the system of...
3	Analýza karyotypu vakonošů (Psychidae, Lepidoptera) metodami klasické a molekulární cytogenetiky FLEGROVÁ, Martina January 2017 (has links) Due to their phylogenetic position, Psychidae play an important role in the investigation of the W chromosome origin in Lepidoptera. Several species of Psychidae were tested for the presence of sex-chromatin and investigated via comparative genomic hybridization. Furthermore, odd chromosome numbers and a Z univalent were observed in females. Overall, this study brings tangible evidence for the absence of the W chromosome in Psychidae, thus contributes to complex knowledge of the W chromosome evolution. In addition, karyotypes of the given species were analyzed using 18S rDNA and histone H3 probes. The results indicate relative stability of their karyotypes.
4	Die Regulation der humanen H3-Histongene / The regulation of the human histone H3 genes Kössler, Heiner 06 November 2003 (has links) No description available. 570 Biowissenschaften, Biologie Mathematics and Computer Science Histon H3 Histongene Histongen-Expression Genregulation Transkriptionsregulation Promotoranalyse Tumorzelllinien Epigenetik CCAAT-Box CBF/NF-Y histone H3 histone gene histone gene expression gene regulation transcriptional regulation promoter analysis tumor cell lines epigenetics CCAAT-box CBF/NF-Y 42.13 42.15 WF

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