No description available.

Cognition of the hydraulic properties of soils is important in civil and environmental projects such as for water supply, geotechnical investigations and evaluation of pollutant spreading. This pilot project aims to develop and test a new method for characterization of the homogeneity and isotropy of different soils by using geophysical measurements. The method is based on geoelectrical (resistivity) measurements using a radial array. Using a radial array with electrode lines in various orientations crossing a midpoint at which water was infiltrated, it was possible to build a 3D-block model showing the water penetration into a soil body. Based on the infiltration pattern it was possible to evaluate the hydraulic heterogeneity and anisotropy of the material. Several common Swedish types of soils were tested. Measurement and evaluation techniques including software were developed. The project shows that geoelectrical measurements using a radial array and geoelectrical instruments available on the market can be used for rapid evaluation of the hydraulic heterogeneity and anisotropy of soils and rock.

Background: In developing the PB-50 word lists, J. P. Egan suggested five developmental principles, two of which were "equal average difficulty" and an "equal range of difficulty" among the lists (page 963). Egan was satisfied that each of the 20 PB-50 lists had equivalent ranges of recognition performances and that the lists produced the same average performances. This was accomplished in preliminary studies that measured the recognition performance of each word and eliminated words that were always or never correct. In preparing for studies of interrupted words, we needed to know the range of difficulty inherent in the speaker specific NU-6 and Maryland CNC materials we planned to use when those words were not interrupted. There were only a few studies in the literature that touched on the range of difficulty characteristic of the word-recognition materials in common usage. The paucity of this information prompted this investigation whose scope broadened to include the CID W-22, Maryland CNC, NU-6, and PB-50 materials spoken by a variety of speakers. Purpose: The purpose was to evaluate the homogeneity with respect to intelligibility of the words that comprise several of the common word-recognition materials used in audiologic evaluations. Research Design: Both retrospective (10) and prospective (3) studies were involved. Data from six of the retrospective studies were from our labs. The prospective studies involved both listeners with normal hearing for pure tones and listeners with sensorineural hearing loss. Study Sample: The sample sizes for the 13 data sets ranged from 24 to 1,030, with 24 the typical number for listeners with normal hearing. Data Collection and Analysis: The retrospective data were from published studies and archived data from our laboratories. The prospective studies involved presentation of the word-recognition materials to the listeners at a comfortable level. An item analysis was conducted on each data set with descriptive statistics used to characterize the data. Additionally, skewness coefficients were calculated on the distributions of word performances and the interquartile range was used to determine minor and major outliers within each set of 200 words and their component 50-word lists (300 words for the Maryland CNCs). Results: For listeners with normal hearing the majority of performances on the words within a 50-word list were better than the mean performance, which produced negatively skewed distributions with outlier performances in every list. For listeners with sensorineural hearing loss the performances on the words within a 50-word list were evenly distributed above and below the mean performance, which yielded essentially normal distributions with few outliers. There were a few words on which performances were better by the listeners with hearing loss. Conclusions: Every list of word-recognition materials has a few words on which recognition performances are noticeably poorer than performances on the majority of the remaining words. If the intention of an experiment is to evaluate performance at the word level, then identifying these "outliers" becomes a necessity. Although not evaluated in this report, the implications for 25-word lists are they should be based on recognition-performance data and not compiled arbitrarily.

No description available.

No description available.

No description available.

The ecotoxicological literature reveals that countless researchers worldwide rely upon informally identified commercial earthworm stocks for laboratory bioassays. The primary aim of this study was to investigate laboratory and commercial stocks of Eisenia species used in South Africa in order to confirm their taxonomy, assess their levels of genetic richness and differentiation. To do so, populations of potential Eisenia andrei and Eisenia fetida were purchased/obtained from vermiculturists and laboratories from four provinces of South Africa. DNA barcoding was used to investigate these taxonomic uncertainties. The COI gene was partially amplified and sequenced in selected earthworms from eight local populations (focal groups) and two European laboratory stocks (non-focal groups). Only nine COI haplotypes were identified from the 224 sequences generated. One of these haplotypes was found to belong to the Megascolecidae Perionyx excavatus. The remaining eight haplotypes belonged to the genus Eisenia although only a single Eisenia fetida haplotype, represented by six specimens, was found in one of the European populations. The other seven haplotypes, all occurring in South Africa, were Eisenia andrei. No Eisenia fetida was found in the South African based populations. One of the commercial stocks from South Africa and a laboratory culture from Europe were mixes of E. andrei - P. excavatus and E. andrei – E. fetida respectively. COI haplotype numbers were limited to two to three distinct sequences within each of the local groups. This translated into a haplotype diversity (H) lower than 0.45 in all the populations, which is very low when compared to other such earthworm studies in which COI polymorphism has been investigated. Of all the local populations investigated, only the lone field population included was genetically divergent from the other populations. This was explained by the haplotype distribution across the populations which indicated that this population was the only one not harbouring the haplotype which represented 75% or more of the COI sequences within the local populations. Because research suggests that earthworm populations with limited genetic diversity may suffer inbreeding depression which could affect traits such as reproduction and survival, the secondary aim was to test whether metal-sensitive earthworms were overly present in the populations investigated. To do so, the three most common COI haplotypes identified between the 8 local populations of E. andrei (called Hap1, Hap2 and Hap3) were paired up and exposed to cadmium. A total of six couples were exposed to 0, 25, 50 and 100 mg Cd/kg for 4 weeks at 20ºC. The survival, biomass variation, cocoon production and cocoon hatching success were assessed for all the couples. The results indicated that couple 6 (Hap3xHap3) was the most sensitive for three of the endpoints assessed whereas couple 4 (Hap1 x Hap3) was the least sensitive. Cocoon hatching success could not help differentiate the couples. The analysis of Cd tissue contents revealed that with increasing Cd concentration, Cp6 (Hap3xHap3) could accumulate significantly more Cd than any other couple (p ≤ 0.01). These findings indicate that earthworm populations may carry intrinsically metal-tolerant and metal-sensitive genotypes. In the context of ecotoxicological testing, the present results underline the importance of using genetically diverse populations in laboratory testing as Cp6 (Hap3xHap3) could have suffered from the deleterious effect of inbreeding. Because E. fetida could not be found in the local populations assessed, it is recommended that further earthworm DNA barcoding studies, covering a more representative geographical area of South Africa and including more field populations of Eisenia spp. be conducted. Because of the occurrence of genetic homogeneity in the populations studied, it is suggested that captive breeding initiatives be established using specimens obtained from several geographically distant field and reared populations. Further research investigating patterns of Cd accumulation/excretion kinetics between the Cd-tolerant and Cd-sensitive individuals reported in the present study, should be conducted to help determine whether inbreeding is the sole factor explaining the observed genotypic responses to Cd. Finally, the necessity of a standardised earthworm barcoding protocol that could help both to properly identify laboratory earthworm stocks and to select genetically diverse stocks suitable for laboratory testing, is discussed together with the relevance of the present work to ecotoxicological testing in general. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2015

The ecotoxicological literature reveals that countless researchers worldwide rely upon informally identified commercial earthworm stocks for laboratory bioassays. The primary aim of this study was to investigate laboratory and commercial stocks of Eisenia species used in South Africa in order to confirm their taxonomy, assess their levels of genetic richness and differentiation. To do so, populations of potential Eisenia andrei and Eisenia fetida were purchased/obtained from vermiculturists and laboratories from four provinces of South Africa. DNA barcoding was used to investigate these taxonomic uncertainties. The COI gene was partially amplified and sequenced in selected earthworms from eight local populations (focal groups) and two European laboratory stocks (non-focal groups). Only nine COI haplotypes were identified from the 224 sequences generated. One of these haplotypes was found to belong to the Megascolecidae Perionyx excavatus. The remaining eight haplotypes belonged to the genus Eisenia although only a single Eisenia fetida haplotype, represented by six specimens, was found in one of the European populations. The other seven haplotypes, all occurring in South Africa, were Eisenia andrei. No Eisenia fetida was found in the South African based populations. One of the commercial stocks from South Africa and a laboratory culture from Europe were mixes of E. andrei - P. excavatus and E. andrei – E. fetida respectively. COI haplotype numbers were limited to two to three distinct sequences within each of the local groups. This translated into a haplotype diversity (H) lower than 0.45 in all the populations, which is very low when compared to other such earthworm studies in which COI polymorphism has been investigated. Of all the local populations investigated, only the lone field population included was genetically divergent from the other populations. This was explained by the haplotype distribution across the populations which indicated that this population was the only one not harbouring the haplotype which represented 75% or more of the COI sequences within the local populations. Because research suggests that earthworm populations with limited genetic diversity may suffer inbreeding depression which could affect traits such as reproduction and survival, the secondary aim was to test whether metal-sensitive earthworms were overly present in the populations investigated. To do so, the three most common COI haplotypes identified between the 8 local populations of E. andrei (called Hap1, Hap2 and Hap3) were paired up and exposed to cadmium. A total of six couples were exposed to 0, 25, 50 and 100 mg Cd/kg for 4 weeks at 20ºC. The survival, biomass variation, cocoon production and cocoon hatching success were assessed for all the couples. The results indicated that couple 6 (Hap3xHap3) was the most sensitive for three of the endpoints assessed whereas couple 4 (Hap1 x Hap3) was the least sensitive. Cocoon hatching success could not help differentiate the couples. The analysis of Cd tissue contents revealed that with increasing Cd concentration, Cp6 (Hap3xHap3) could accumulate significantly more Cd than any other couple (p ≤ 0.01). These findings indicate that earthworm populations may carry intrinsically metal-tolerant and metal-sensitive genotypes. In the context of ecotoxicological testing, the present results underline the importance of using genetically diverse populations in laboratory testing as Cp6 (Hap3xHap3) could have suffered from the deleterious effect of inbreeding. Because E. fetida could not be found in the local populations assessed, it is recommended that further earthworm DNA barcoding studies, covering a more representative geographical area of South Africa and including more field populations of Eisenia spp. be conducted. Because of the occurrence of genetic homogeneity in the populations studied, it is suggested that captive breeding initiatives be established using specimens obtained from several geographically distant field and reared populations. Further research investigating patterns of Cd accumulation/excretion kinetics between the Cd-tolerant and Cd-sensitive individuals reported in the present study, should be conducted to help determine whether inbreeding is the sole factor explaining the observed genotypic responses to Cd. Finally, the necessity of a standardised earthworm barcoding protocol that could help both to properly identify laboratory earthworm stocks and to select genetically diverse stocks suitable for laboratory testing, is discussed together with the relevance of the present work to ecotoxicological testing in general. / MSc (Environmental Sciences), North-West University, Potchefstroom Campus, 2015

The study seeks to determine the effect upon the F-statistic of violating the assumption of homogeneity of regression slopes in the one-way, fixed-effects analysis of covariance model. The study employs a Monte Carlo simulation technique to vary the degree of heterogeneity of regression slopes with varied sample sizes within experiments to determine the effect of such conditions. One hundred and eighty-three simulations were used.

The Charles University Faculty of Science KFGG team took part in an intensive research in the area of Kyrgyz glacial lakes. The collecting of records from three meteorological stations in the locations of Kolor (2,700 m above sea level) and Adygine (3,500 and 3,800 meters above sea level) was part of the research. The aim of this thesis was to analyze the homogeneity of climatic data measured in experimental locations using the SNHT method (Standard Normal Homogeneity Test), process the data using general climatological processes, create a classification of circulation types for the area of interest using Jenkinson's and Collinson's method, and finally, to quantify the relationships between individual circulation types and the values of chosen climatic elements. The analysis results are homogenized climatic sets for the Adygine H station (3,800 m above sea level) and a summary of basic statistics and trends of climatic elements in the area. Furthermore, a catalogue of circulation types was created for the period from August 2007 to July 2011, and finally, the relationships between individual circulation types and manifestations of climate elements (air temperature, precipitation and global radiation). The results of this thesis may be used to identify the types connected with bursts of glacial...