Thesis (MscAgric (Horticulture))--University of Stellenbosch, 2006. / Protea, Leucadendron and Leucospermum belong to the Proteaceae family. These three genera form an important part of the floriculture industry in South Africa and are commonly used as fresh cut flowers or dried flowers for both the local and international market. The distribution of macro and micro - nutrients was investigated in Protea ‘Cardinal’ using rooted cuttings grown from October 2001 to March 2002. The plants were divided into 1st flush leaf and stem, 2nd flush leaf and stem, basal leaf and basal stem, roots and the flower bud. These tissues were analysed to determine N, P, K, Ca, Mg, Fe, S, Na, B and Mn concentration. Results indicated that N decreased over time in all tissues and accumulated more in leaves than in stems. P in leaves and stems increased with time, while K increased in leaf tissues and remained stable in stem tissue. Ca did not change in young leaves but was high in old leaves. Mg in leaves increased but in basal stem, 1st flush stem and roots Mg decreased over time. Fe in leaves and roots increased with time and not a less significant change occurred in stems. In most tissues, B and Na decreased with time. S increased in leaves and decreased in stems with time. Mn was relatively stable in most tissues except in basal leaves where it increased. Nutrient concentration in tissues, especially in leaves, showed no distinct pattern in the distribution of nutrients. Eleven cultivars from the three genera, namely Protea, Leucadendron and Leucospermum, were used to develop deficiency symptoms of macro - nutrients by using different nutrient compositions. The plants were grown in 20cm pots from December 2002 to September 2003. The eleven cultivars were chosen because of their high market value. Visual symptoms were recorded in two stages with a five - month interval for each stage. The first stage was recorded from December 2002 to April 2003 and the second stage was recorded from May 2003 to September 2003. Observed symptoms indicated significant differences between the control and treatments in which specific nutrients were withheld. Some cultivars exhibited some symptoms that are commonly noticed under field conditions, e.g. in the N deficiency treatment the upper leaves of Protea ‘Sylvia’ were reddish and the lower leaves were chlorotic especially at the later stage. Protea ‘Red Rex’ in the P deficiency treatment had random red tints around the leaf including the petiole, these symptoms are also commonly noticed in Protea ‘Red ‘Rex’ in field conditions. Leucadendron ‘Rosette’ also showed some uncommon symptoms of accumulating “sugar” on leaf tips in Ca deficiency treatment that was not observed in any other cultivar. In some cultivars the symptoms were systematic e.g. Leucadendron ‘Chameleon’ while in other cultivars the deficiency affected a certain leaf age e.g. Leucospermum ‘High Gold’ in the Ca deficiency treatment. The buds in 'High Gold' died prematurely in the Ca deficiency treatment. Protea ‘Cardinal’ was used to determine the optimal N and P source and concentrations for optimal growth. Protea ‘Cardinal’ was grown in a temperaturecontrolled glasshouse for seven months using silica sand as growth medium. Different levels and sources of N and P were applied. The N was applied in a complete nutrient solution as NH4 +, NO3 -, NH4 +: NO3 - (1: 1, 1: 4 and 4: 1 ratios) and Urea, at different concentration levels: 5 mM, 1 mM and 0.1 mM. P was applied at 1 mM, 0.1 mM and 0.01 mM. The plants were fertigated manually with 1L nutrient solution on every second day of the week. P at 0.01 mM and 0.1 mM resulted in optimal plant growth. The 1 mM P resulted in marginal leaf scorching or dryness. When N was applied at 5 mM plant growth was more optimal than when N was applied at 1 mM and 0.1 mM. More dry weight was accumulated at 5 mM than at 1 mM or 0.1 mM N. The dry weight of leaves, stem and roots tissues at 5 mM were higher in the NO3 - treatment.

Thesis (MScAgric (Horticulture))--University of Stellenbosch, 2009. / ENGLISH ABSTRACT: In order to develop a novel pink- or red-fleshed apple for the fresh consumer market,the red-fleshed genotype, ‘KAZ 91’ (Malus niedzwetzkyana Dieck.), was crossed with ‘Meran’ (M. domestica Borkh) at the Agricultural Research Council (ARC) apple breeding programme in South Africa. The objective of this study was to evaluate and quantify colour variability in the flesh and peel of this progeny (Family 1), to assess quality traits (i.e., acidity and total soluble solids) and phenolic levels in fruit peel and flesh, and to investigate consumer preference for the taste and appearance of redfleshed apples. All data, except for consumer preference, were also collected on two white-fleshed M. domestica Borkh families, i.e., ‘Reinette Burchardt’ x ‘Treco Red Gala’ (Family 2) and ‘Meran’ x ‘Treco Red Gala’ (Family 3). Fruit of Family 1 seedlings were on average darker red with greater blush coverage and higher anthocyanin and phenolic levels compared to fruits of Family 2 and 3. The proportion of bearing trees with red-fleshed fruits in Family 1 increased from 25% in 2007 to 35% in 2008. The intensity and distribution of red pigmentation in the flesh varied considerably between seedlings and even between individual apples from the same tree. A high intraclass correlation coefficient was found for red-flesh coverage within Family 1, indicating a high level of genetic determination that can be used in breeding. When only red-fleshed seedlings were considered, an intermediate repeatability coefficient (0.54) for red-flesh coverage indicates that the extent of red flesh coverage varies to some extent between seasons. The effect of environmental factors on red flesh colour needs to be assessed and breeders need to take care to select for genotypes with stable flesh colour intensity and coverage. Small intraclass correlations were found between families for other traits. No correlation was found for anthocyanin and total phenolics in both peel and flesh or between anthocyanin levels in the peel and flesh of Family 1 fruit. This suggests that red-fleshed fruit will not necessarily be high in antioxidants – since phenolics is by far the greatest contributor to fruit antioxidant capacity. The lack of a correlation between peel and flesh anthocyanin levels also suggest that fruit with dark red flesh will not necessarily have a dark red skin colour. Family 1 fruit were more prone to flesh browning and were more acidic compared to fruit of Families 2 and 3. With regard to consumer preference for flesh colour, 74% of South African consumers preferred white flesh while 64% preferred an attractive “floral” pattern created by the combination of a red cortex and white core. Consumers indicated a much lower liking for other distribution patterns and lower intensities of red flesh colour. Consumers preferred the taste of apples that were crisp, crunchy and high in apple flavour irrespective of flesh colour. Red-fleshed fruit were generally acidic or had poor texture, and some were also astringent. However, despite a general dislike in acidic fruit, consumers showed a preference for acidic fruit if that fruit also had high red-flesh coverage. / AFRIKAANSE OPSOMMING: Appeltelers van die Suid-Afrikaanse Landbounavorsingsraad (LNR) Infruitec- Nietvoorbij poog om unieke appelkultivars met ‘n pienk of rooi vleiskleur te ontwikkel. Vir hierdie doel het hulle die rooivleis genotipe, ‘KAZ 91’ (Malus niedzwetzkyana Dieck), gekruis met ‘Meran’ (M. domestica Borkh). Hierdie studie is uitgevoer ten einde kleurvariasie, interne kwaliteitseienskappe (i.e., suurheid en totale oplosbare vastestowwe) en vlakke van fenole in die vleis en skil van bogenoemde kruisingkombinasie se nageslag (Familie 1) te evalueer en te kwantifiseer asook om verbruikersvoorkeure vir die smaak en voorkoms van rooivleis appels te bestudeer. Buiten vir verbruikersvoorkeure, is alle data ook ingesamel vir twee witvleis M. domestica families, naamlik ‘Reinette Burchardt’ x ‘Treco Red Gala’ (Familie 2) en ‘Meran’ x ‘Treco Red Gala’ (Familie 3). Familie 1 saailinge se vrugte was gemiddeld donkerder rooi met ‘n groter rooi blos en hoër antosianien- en fenoolvlakke in vergeleke met vrugte van Families 2 en 3. Van die Familie 1 saailinge wat wel vrugte gedra het, het 25% en 35% in onderskeidelik 2007 en 2008 vrugte met rooi vleis gehad. Die intensiteit en verspreiding van rooi pigmentasie in die vleis het aansienlik varieer tussen saailinge en selfs tussen individuele appels van dieselfde boom. ‘n Hoë intraklas korrelasie koëffisiënt is gevind vir die proporsie van die vleis met rooi pigmentasie in Familie 1 nageslag. Dit dui op ‘n hoë vlak van genetiese determinasie en vinnige vordering met teling vir hierdie eienskap. Indien net rooi-vleis saailinge egter oorweeg word, word ‘n intermediêre herhaalbaarheid koëffisiënt (0.54) vir die proporsie van die vleis wat rooi is verkry, wat dui op aansienlike variasie tussen seisoene in die omvang van rooi pigmentasie. Die effek van omgewingsfaktore op rooi vleiskleur behoort dus bestudeer te word en telers moet let daarop om te selekteer vir genotipes met stabiele vleiskleur intensiteit en bedekking. Die intraklas korrelasies tussen families vir ander vrugeienskappe was klein. Antosianienvlakke en totale fenole in die skil en vleis van Familie 1 vrugte het nie gekorreleer nie. Dit dui daarop dat rooivleis appels nie noodwendig ‘n hoër antioksidantkapasiteit het nie – fenole maak by verre die grootste bydrae tot die antioksidantkapisiteit van vrugte. Antosianienvlakke in die skil en vleis van Familie 1 vrugte het ook nie gekorreleer nie wat daarop dui dat vrugte met ‘n donker rooi vleis nie noodwendig ook ‘n donker skilkleur sal hê nie. Familie 1 vrugte was gemiddeld suurder as vrugte van Families 2 en 3 en Familie 1 vrugte se vleis het gemiddeld ook meer verbruining ondergaan. Ten opsigte van verbruikersvoorkeur vir vleiskleur is gevind dat 74% van verbruikers ‘n wit vleiskleur verkies het terwyl 64% gehou het van die aantreklike “blom” patroon gevorm deur ‘n rooi korteks en wit kern. Verbruikers het aansienlik minder gehou van enige ander verspreiding van rooi pigment in die vleis of van ‘n laer intensiteit rooi kleur. In terme van smaak is geurige appels met ‘n bros tekstuur verkies ongeag hul vleiskleur. Rooivleisappels was oor die algemeen suur met ‘n swak tekstuur. Sommige rooivleisappels was ook frank. Tog, ten spyte van ‘n algemene afkeur in suur appels, het verbruikers ‘n voorkeur getoon vir vrugte met ‘n kombinasie van hoë suur en ‘n hoë proporsie vleis met rooi pigmentasie.

Thesis (MSc (Horticulture))--University of Stellenbosch, 2007. / This study was undertaken to quantify some of the effects of daily fertigation on ecophysiological responses in citrus trees (Citrus spp.). Initial research was conducted to optimise and standardise the sampling procedure to quantify stem water potential (ψstem) in citrus trees. To reliably determine the plant water status of citrus trees, the following conditions are required to minimise unwanted variation in ψstem measurements. Bagging of leaves with black polyethylene envelopes covered with aluminium foil 3 to 4 hours prior to measuring ψstem allows the plant water status in those leaves to equilibrate with whole-tree plant water status, thereby providing a realistic measurement of the current water status. The use of aluminium foil to cover the bagged leaves, reduces unwanted heat stress by reflecting sunlight, and dramatically reduced variation in ψstem. The time of day at which ψstem measurements are made is important to ensure consistency in comparisons among treatments and interpretation of irrigation treatment effects. “Physiological midday” is the preferred time of day to measure ψstem, i.e. 1100 HR. Transpiring leaves with open stomata would be in sun-exposed positions on the east side of trees and should be used for making ψstem measurements. Under similar experimental conditions as those used here, only three leaves per replicate are required to detect a difference of 0.05 MPa in ψstem between treatment means. Plant water status categories were developed which may have useful practical applications, i.e. >-1.0 MPa = no water deficit; -1.0 to -1.2 MPa = low water deficit; -1.2 to -1.4 MPa = moderate water deficit; <-1.4 to -1.6 MPa = high water deficit; and <-1.6 MPa = severe water deficit. Attempts are being made to develop systems that improve crop management and enhance citrus fruit production through efficient and timeous application of water and mineral nutrients which has led to the use of daily drip fertigation or the open hydroponics system (OHS). However, the perceived benefits are not necessarily supported by facts. Fruit size and yield are apparently enhanced, but possible negative aspects of the system have not been quantified. Fruit produced on trees grown under daily drip fertigation generally have a lower total soluble solids concentration than on trees under micro-sprinkler irrigation. This is mainly due to a dilution effect that is caused by the greater availability of water and the uptake thereof. Sugar accumulation can be optimised by controlling the amount of water that the plant receives at different developmental stages. Therefore, it is essential to quantify the ecophysiological responses and benefits of OHS/daily fertigation, as well as the effects of this technology on fruit quality. ‘Nules Clementine’ mandarin (C. reticulata Blanco) trees in two commercial orchards in Simondium, Western Cape province, South Africa, received differential irrigation treatments. The treatments were applied at the end of stage I (± mid December) of fruit development. Stem water potential, fruit size and internal fruit quality were determined. Water-deficit stress enhanced sugar accumulation of ‘Nules Clementine’ mandarin by 0.3 to 0.6 °Brix under certain conditions. These conditions require that the difference in ψstem should be of a sufficient intensity of between 0.16 and 0.3 MPa, and this difference should be maintained for a sufficient duration of between 4 and 6 weeks. Furthermore, deficit irrigation should be applied relatively early in fruit development, namely during the sugar accumulation stage which starts within 4 weeks of the end of the fruit drop period and continues until harvest.

Thesis (MScAgric (Horticulture))--Stellenbosch University, 2008. / Chemical thinning of fruit trees has become a central management practice for ensuring high fruit quality at harvest and return bloom the following season. Three trials were conducted in the 2004/5, 2006/7 and 2007/8 seasons to investigate the efficacy and mode of action of chemical thinning agents on European pear cultivars (Pyrus communis L) in the Western Cape, South Africa. The first trial was conducted in the 2004/5 and 2006/7 seasons to evaluate the efficacy of 50, 100 and 150 mg.l-1 6-benzyladenine (BA), and 30 and 40 mg.l-1 naphthylacetamide (NAD) on ‘Early Bon Chrétien’ pear. BA was more effective than NAD in reducing crop load and improving fruit size. Crop load decreased and fruit size increased with increasing rate of BA. BA significantly improved, whilst NAD failed to improve return bloom. In the second trial, three experiments were conducted in the 2006/7 and 2007/8 seasons to evaluate the efficacy of 100 to 200 mg.l-1 BA on ‘Forelle’ pear. The first experiment was conducted in the 2006/7 season where BA rates of 100, 125 and 150 mg.l-1 generally failed to reduce crop load or to improve fruit size and fruit size distribution and return bloom. The second experiment was conducted in the 2007/8 season where two BA rates, 150 and 200 mg.l-1 and a split-application of 3 x 50 mg.l-1 improved fruit size. The 200 mg.l-1 rate was the most effective treatment. BA did not improve fruit size distribution and return bloom. The third experiment was conducted in the 2007/8 season where the effect of rate and timing of BA applications was evaluated. Two rates, 150 and 200 mg.l-1 were applied 8, 11 and 17 days after full bloom (d.a.f.b.). There was no significant interaction between BA rate and application time. The 200 mg.l-1 rate and the 11 d.a.f.b. (i.e. 8 to 10 mm average fruit size) applications were more effective in reducing crop load, and improving fruit size. BA at 150 and 200 mg.l-1 and at all application times significantly improved return bloom relative to the control. From these trials we concluded that BA is a reliable thinner for ‘Early Bon Chrétien’ at rates of 100 or 150 mg.l-1. On ‘Forelle’, BA is not a reliable thinner and we recommended further trials with BA in combination with other thinning agents. In the third trial, three experiments were conducted in the 2007/8 season to investigate the mode of action and effect of BA application time on European pear cultivars. The effect of site of application, bourse shoot growth and fruit size at time of application on the efficacy of BA was evaluated. Results from the experiments on the effect of site of application and bourse shoot growth were inconclusive. In terms of fruit abscission, there was a significant interaction between BA application time and fruitlet size. Early BA applications (8 d.a.f.b.) were significantly more effective in promoting fruit abscission, than later (11 and 17 d.a.f.b.) applications. Smaller fruit (6 to 8 mm) were found to be more susceptible to BA-induced fruit abscission than bigger fruit (8 to 12 mm).

Thesis (MScAgric (Horticulture))--University of Stellenbosch, 2010. / ENGLISH ABSTRACT: Persimmon production is new to South Africa with about 700 ha planted to the dioecious, parthenocarpic Triumph cultivar since 1998. Little local expertise is available to assist growers in achieving high yields of high quality fruit and previous research has shown that recipes that are followed in Israel, from where ‘Triumph’ was introduced to South Africa, do not necessarily have any beneficial effect in South Africa. ‘Triumph’ orchards in South Africa are often late in reaching full production. Persimmon trees are generally vigorous and prone to excessive fruit drop, partly due to excessive vegetative growth, especially when young and grown on the very vigorous Diospyros lotus seedling rootstock. The first objective of this study was to evaluate the use of growth retardants and various severities of girdling to increase flower formation, fruit set and yield in vigorous, young ‘Triumph’ orchards. Scoring and girdling improved fruit set and yield in two such orchards and are recommended as tools to improve yield in ‘Triumph’ in South Africa. Strapping, prohexadione-Ca (P-Ca) and paclobutrazol (PBZ) did not increase yield whereas 5 mm bark removal was too severe a treatment and decreased fruit quality in the current season and yield in the following season. None of the treatments had an effect on flower formation or decreased vegetative growth. PBZ, especially as foliar spray, appears to advance fruit maturity. P-Ca at 125 mg L-1 and 250 mg L-1 induced phytotoxicity symptoms and decreased yields in both orchards. However, further research is required before P-Ca and PBZ are completely discarded as treatments to manage vigor in ‘Triumph’ persimmon in South Africa. In contrast to the negative effect of excessive vigor on fruit production, the profitability of orchards is dependent on the rapid growth of trees after planting in order to fill the allotted canopy volume and achieve full production as quickly as possible. Hence, the second objective of this study was to determine optimum levels of irrigation and fertilizer application rates to attain early, high yields in newly planted ‘Triumph’. Fertigation was applied at three levels, viz. ½X, 1X and 2X with 1X being the commercial standard application rate. Irrigation was also applied at these levels without addition of fertilizer. In addition, fertilizer was applied at 0X, ½X and 1X at 1X irrigation level. Tree size increased with an increase in water application rate. Yield also increased linearly with an increase in water application rate due to a linear increase in fruit size. Fertigation and ½X water as well as an increase in fertilizer application rate at 1X irrigation substantially delayed fruit ripening. Hence, careful management of fertilizer and water application rate could be used to extend the harvesting period and, therefore, the marketing window of South African ‘Triumph’. We recommend that the trial be continued for a further few seasons so that the effect of water and fertilizer application rates on fruit quality and storability can be assessed. Fruit set may also be affected as trees reach their mature size with a concomitant increase in shading. / AFRIKAANSE OPSOMMING: Persimmonverbouing is ‘n nuwe bedryf in Suid-Afrika met ongeveer 700 ha van die tweeslagtige, partenokarpiese Triumph cultivar wat sedert 1998 aangeplant is. Min plaaslike kundigheid is beskikbaar om produsente van raad te bedien oor hoe om te werk te gaan om hoë opbrengste van hoë kwaliteit te verkry. Vorige navorsing het getoon dat resepte wat ‘Triumph’ van Israel na Suid-Afrika gevolg het, nie noodwendig suksesvol hier toegepas kan word nie. ‘Triumph’ boorde in Suid-Afrika neig om lank te neem alvorens hul hul maksimum produksievermoë bereik. Persimmons is oor die algemeen baie groeikragtig en geneig tot hoë vrugval, deels as gevolg van hul geil groei, en veral terwyl hulle jonk is en op die uiters groeikragtige Diospyros lotus saailingonderstam geënt is. Die eerste doelwit van hierdie studie was om die invloed van groei inhibeerders en verskillende grade van strafheid van ringelering op blomvorming, vrugset en oesopbrengs in jonk, sterk-groeiende ‘Triumph’ boorde te evalueer. Insnyding en ringelering met ‘n handsaag het vrugset en oeslading in twee groeikragtige boorde verbeter en word aanbeveel as geskikte ingrepe om die oeslading van ‘Triumph’ te verhoog. Draad-ringelering, en aanwending van prohexadione-Ca (P-Ca) en paclobutrazol (PBZ) het nie die opbrengs verhoog nie terwyl die verwydering van `n 5 mm strook bas té aggresief was en die vrugkwaliteit in die seisoen van toediening en opbrengs in die daaropvolgende seisoen verlaag het. Geen van die behandelings het blomvorming geaffekteer of vegetatiewe groei verminder nie. Dit wil voorkom asof PBZ, veral as blaartoediening, vrugrypwording kan versnel. Blare het tekens van fitotoksisiteit getoon na aanwending van P-Ca teen 125 mg L-1 en 250 mg L-1. P-Ca het ook die opbrengs in beide boorde aansienlik verlaag. Verdere navorsing is egter nodig alvorens P-Ca en PBZ sondermeer verwerp word as behandelings om die groei van ‘Triumph’ te beheer. Die winsgewendheid van boorde is afhanklik daarvan dat bome aanvanklik vinnig groei ten einde die toegekende boomryvolume so spoedig moontlik te vul en sodoende so vinnig as moontlik hul vol produksievermoë bereik. Bogenoemde is natuurlik teenstrydig met die negatiewe effek van uitermatige geil vegetatiewe groei op vrugproduksie. Die tweede doelwit van hierdie studie was dus om die optimale vlakke van besproeiing en bemesting te bepaal wat die vroeë aanvang van hoë opbrengste in nuwe ‘Triumph’ boorde sal verseker. Vloeibare bemesting is in kombinasie met besproeiing teen drie vlakke toegedien nl. ½X, 1X en 2X met 1X die kommersiële standaard vlak van toediening. Besproeiing is ook teen hierdie vlakke toegedien sonder dat kunsmis bygevoeg is. Addisioneel hiertoe is bemesting ook toegedien teen ½X, 1X en 2X teen 1X besproeiing. Boomgrootte het toegeneem met `n toename in die vlak van besproeiing. `n Lineêre toename in vruggrootte met ‘n toename in die vlak van besproeiing het ‘n oorsaaklike lineêre toename in opbrengs tot gevolg gehad. Bemesting in kombinasie met besproeiing, ½X besproeiing sonder bemesting, asook `n toename in die bemestingsvlak by 1X besproeiing het vrugrypwording substansieel vertraag. Die omsigtige bestuur van bemesting- en besproeiingsvlakke kan moontlik gebruik word om die oesperiode, en dus die bemarkingsvenster, vir Suid-Afrikaanse ‘Triumph’ te verleng. Ons beveel aan dat die proef vir ‘n vêrdere aantal seisoene voortgesit word sodat die effek van bemesting- en besproeiingsvlakke op vrugkwaliteit en -houvermoë bepaal kan word. Verhoogde oorskaduwing soos wat bome van sekere behandelings hul toegekende spasie bereik en oorskry, kan ook in die toekoms ‘n invloed op vrugset uitoefen.

Thesis (MScAgric) -- University of Stellenbosch, 2000. / ENGLISH ABSTRACT: Postharvest berry split and abscission are prevailing physiological disorders that negatively impact on the quality of table grapes exported from South Africa. Inferior grape quality due to these disorders results in a considerable decline in consumer confidence in the branded product, which leads to a drop in demand, and consequently, lower prices. Since information concerning postharvest factors influencing postharvest berry split and abscission is limited, the search for reliable methods to adequately control these problems remains elusive. In an attempt to obtain the required information, the influence of harvest temperature, harvest maturity, perforated liners, field heat removal prior to packing, delay periods before and after packing, storage duration and the elevation of storage temperature on the development of berry split and abscission in 'Thompson Seedless' (Vitis vinifera Linnaeus) table grapes was investigated. Changes in abscission related factors during berry development, and the influence of pre-and postharvest ethylene inhibitors on the development of berry abscission in 'Waltham Cross' table grapes, was also studied. Berry split was aggravated by packing 'Thompson Seedless' grapes at high pulp temperatures of approximately 30°C, especially if the grapes were packed in non-perforated bags. The incidence of berry split could be reduced by between 80 and 90% by packing grapes in perforated instead of non-perforated liners. Perforated bags also reduced levels of S02 damage. However, due to significantly more moisture loss from grapes in perforated bags, compared to non-perforated bags, the risk of higher fruit and stem desiccation and softer berries existed. Optimum size and density of perforations needs to be determined to reduce berry split without excessive loss of moisture from the grapes, and S02gas from the air space surrounding the product. The influence of harvest temperature and liner type on berry abscission was not conclusive. Advanced maturity increased grape resistance to berry split. However, grapes harvested too mature were prone to stem desiccation and the development of Botrytis decay. The occurrence of berry abscission also appeared to increase with advanced harvest maturity. Consequently, to ensure optimal post-storage quality, 'Thompson Seedless' grapes should be harvested as soon as horticultural maturity has been reached, which appears to be at approximately 18°Brix. Field heat removal for 1.5 hours at 19°C prior to packing had no beneficial or adverse effect on berry split and abscission. Delay periods prior to packing aggravated berry abscission, but did not influence berry split significantly. Grapes delayed for 12 hours showed a significant increase in berry abscission and Botrytis decay, compared to grapes delayed for only 3 or 8 hours. Considering that the absence of fungal decay is the most important quality prerequisite in table grapes, it is of vital importance to pack grapes with as short a delay period as possible. Grapes packed in non-perforated liners and delayed for different durations after packing, before the onset of forced-air cooling (FAC), showed significant differences regarding the incidence of berry split. Grapes delayed for 18 hours had significantly higher levels of berry split directly after the delay period, compared to grapes delayed for 6 or 12 hours. No significant difference in berry abscission occurred between grapes delayed for different periods. To minimise the amount of berry split, FAC should be applied as rapidly as possible after the packing of grapes in non-perforated liners. Two storage related factors significantly influenced the incidence of berry split in 'Thompson Seedless' grapes during cold storage significantly, viz. the duration of storage at -O.soC,and the increase in temperature after low temperature storage. Berry split increased almost linearly with prolonged storage at -O.soC. An elevation of storage temperature from -O.soC to 10°C any time during the cold storage period, further aggravated the split problem. Consequently, the reduction of berry split in 'Thompson Seedless' table grapes during cold storage requires (a) the shortest possible cold storage period, and (b) good temperature management throughout distribution, from initiation of cooling until the final point of sale. The grape berry abscission potential, as quantitatively indexed by the measurement of the fruit removal force (FRF), showed significant changes during berry development of 'Waltham Cross' table grapes, from 27 to 111 days after full bloom (OAFB). This showed that at certain stages of fruit growth, 'Waltham Cross' grapes are more prone to berry abscission. At 27 OAFB, when the berries had an average diameter of 6.6mm, the grape bunches showed a significantly higher potential for berry abscission, compared to grapes sampled at a later stage. 'Waltham Cross' has inherently straggly bunches with bare shoulders. Therefore, any abscission during berry development will aggravate the problem. Consequently, it is of vital importance that any adverse factors such as moisture stress be avoided, especially during the period when 'Waltham Cross' grapes appear to be very susceptible to berry abscission. Of all parameters measured, moisture loss showed the best correlation with abscission. Grapes harvested with total soluble solids (TSS) of 12.3°Brix, 83 OAFB, had a significantly higher abscission potential than grapes harvested more mature. Therefore, by harvesting 'Waltham Cross' grapes at optimum maturity, at a TSS of approximately 16.4°Brix, berry abscission can be reduced to a great extent. It was evident that at veraison, the metabolism of grape berries changes drastically, and additional to the rapid increase in sugars and the rapid decrease in acidity, a decrease in FRF occurs. Preharvest sprays of ReTain™ (a derivative of aminoethoxyvinylglycine), which inhibits ethylene synthesis, showed no promise as a means to reduce postharvest berry abscission. A postharvest treatment with EthylBloc® (1-methylcyclopropene), which inhibits ethylene action, only reduced berry abscission during one season. / AFRIKAANSE OPSOMMING: Die fisiologiese defekte korrelbars en los korrels wat algemeen voorkom tydens opberging van sekere tafeldruif-kultivars, het 'n negatiewe invloed op tafeldruiwe wat uitgevoer word vanaf Suid-Afrika. Minderwaardige kwaliteit as gevolg van hierdie defekte het 'n aansienlike afname in verbruikers-vertroue tot gevolg wat aanleiding gee tot 'n ooreenkomstige afname in aanvraag en prys van die produk. Inligting rakende na-oes faktore wat die voorkoms van korrelbars en los korrels beïnvloed is beperk, en geen gewaarborgde metode bestaan om hierdie twee defekte volkome te beheer nie. In 'n poging om dié gewenste inligting te bekom, is ondersoek ingestel na die effek van oes-temperatuur, oes-rypheid, geperforeerde sakke, veldhitte verwydering voor verpakking, vertragingsperiodes voor en na verpakking, tydsduur van opberging, en die verhoging van die opbergingstemperatuur, op die voorkoms van korrelbars en los korrels by 'Thompson Seedless' (Vitis vinifera Linnaeus) druiwe. Daar is ook ondersoek ingestel na veranderings in afsnoering verwante faktore tydens korrel-ontwikkeling, en die invloed van vooren na-oes toedienings van etileen inhibeerders op die ontwikkeling van los korrels by 'Waltham Cross'tafeldruiwe. Korrelbars is vererger deur 'Thompson Seedless' met hoë pulptemperature van ongeveer 29.5°C te verpak, veral indien dit in 'n riie-geperforeerde sak verpak is. Die voorkoms van korrelbars kon tussen 80 en 90% verminder word deur 'Thompson Seedless' druiwe in geperforeerde sakke te verpak, in plaas van nie-geperforeerde sakke. Geperforeerde sakke het ook S02 skade op die druiwe verminder. Tog, as gevolg van betekenisvol meer vogverlies vanaf druiwe in geperforeerde sakke as vanaf druiwe in nie-geperforeerde sakke, bestaan die risiko van meer stingel-uitdroging en minder ferm korrels indien druiwe in geperforeerde sakke verpak word. Optimale grootte en digtheid van perforasies moet bepaal word om korrelbars te verminder, maar sonder oormatige vogverlies vanaf die druiwe en oormatige verlies aan S02. Die invloed van oes-temperatuur en sak-tipe op los korrels was nie oortuigend nie. Gevorderde oes-rypheid het die druif se weerstand teen korrelbars verhoog. Daarteenoor was druiwe wat té ryp geoes is, meer gevoelig vir stingel-uitdroging en Botrytis bederf. Dit wilook voorkom of die voorkoms van los korrels toeneem met gevorderde rypheid. Dus, om optimum kwaliteit na opberging te verseker, moet 'Thompson Seedless' geoes word sodra hortologiese rypheid bereik word, wat blyk om by 'n totale opgeloste vaste stof-inhoud (TOVS) van ongeveer 18°Brix te wees. Veldhitte verwydering voor verpakking, vir 1.5 uur by 19°C, het geen effek gehad op die voorkoms van korrelbars en los korrels nie. 'n Vertragingsperiode voor verpakking het die los korrel-probleem vererger, alhoewel dit geen betekenisvolle invloed op die voorkoms van korrelbars gehad het nie. Druiwe wat vir 12 uur voor verpakking vertraag is, het betekenisvol meer los korrels en Botrytis bederf getoon, in vergelyking met druiwe wat slegs 'n vertragingsperiode van 3 of 8 uur ondergaan het. Aangesien die afwesigheid van bederf die belangrikste kwaliteits-vereiste vir tafeldruiwe is, is dit van kardinale belang om druiwe so gou as moontlik na oes te verpak. Druiwe, verpak in nie-geperforeerde sakke, wat vir verskillende periodes vertraag is voor geforseerde-lug verkoeling, het betekenisvolle verskille getoon betreffende die voorkoms van korrelbars. Druiwe vertraag vir 18 ure voor verkoeling, het betekenisvol meer korrelbars getoon, soos gemeet onmiddellik na die vertragingsperiode, in vergelyking met druiwe wat slegs vir 6 of 12 ure vertraag was. Geen betekenisvolle verskille in los korrels het voorgekom tussen druiwe wat verskillende vertragingsperiodes ondergaan het nie. Om korrelbars te verminder, moet geforseerde-lug verkoeling so gou as moontlik na verpakking van druiwe in nie-geperforeerde sakke toegepas word. Twee opbergings-verwante faktore beïnvloed die voorkoms van korrelbars by 'Thompson Seedless' druiwe tydens koelopberging, naamlik die tydsduur van opberging by -O.soC,asook 'n styging in temperatuur vanaf -O.soC tot 1DoC. Korrelbars het feitlik liniêr toegeneem met verlengde opberging by -O.soC. 'n Styging in temperatuur vanaf -O.SoCtot 1DoCop enige tydstip gedurende die koelopbergingsperiode, het korrelbars verder vererger. Dus, om korrelbars by 'Thompson Seedless' tydens opberging tot die minimum te beperk, moet die tydsduur van opberging so kort as moontlik wees, en moet die koue ketting regdeur die distribusie-proses gehandhaaf word, vanaf inisiëring van verkoeling tot en met die uiteindelike verkoop van die produk. Die afsnoerings-potensiaal van druiwe, soos kwantitatief geïndekseer is deur meting van die vrug-verwyderings-vermoë (VVV), het betekenisvol verander gedurende korrel-ontwikkeling van 'Waltham Cross' tafeldruiwe, vanaf 27 tot 111 dae na volblom (DNVB). Dit het getoon dat 'Waltham Cross' druiwe by sekere stadiums van vrug-groei meer gevoelig is vir korrel afsnoering. By 27 DNVB, wanneer die korrels 'n gemiddelde deursnee van 6.6mm gehad het, het die druiwe 'n betekenisvolle hoër potensiaal vir afsnoering getoon, in vergelyking met druiwe wat op 'n latere stadium getoets is. 'Waltham Cross' is inherent geneig tot yl trosse met kaal skouers, gevolglik sal enige afsnoering tydens korrel-ontwikkeling die probleem vererger. Dus is dit van kardinale belang dat enige nadelige faktor, soos byvoorbeeld vogstres, vermy moet word, veral gedurende periodes wanneer dit wil voorkom of 'Waltham Cross' baie vatbaar is vir korrel afsnoering. Van al die parameters wat gemeet is, het vogverlies die beste korrelasie met korrel afsnoering getoon. Druiwe wat 83 DNVB, by 'n TOVS van 12.3°Brix geoes is, het 'n betekenisvol hoër potensiaal vir korrel afsnoering getoon, in vergelyking met druiwe wat ryper geoes is. Dus, deur 'Waltham Cross' druiwe by optimum rypheid te oes, by 'n TOVS van ongeveer 16.4°Brix, kan korrelbars in 'n groot mate verminder word. Tydens verelson, wanneer die metabolisme van die druiwe drasties verander, was daar gepaardgaande met die drastiese toename in TOVS en die drastiese afname in totale titreerbare sure (TSS), ook 'n afname in Voor-oes bespuitings met ReTain™, wat etileen sintese inhibeer, het geen potensiaal getoon om los korrels by 'Waltham Cross' te verminder nie. 'n Na-oes behandeling met EthyIBloc®, wat etileen werking inhibeer, het slegs korrel afsnoering in een van die seisoene effens verminder.

Thesis (MScAgric) -- University of Stellenbosch, 2002. / ENGLISH ABSTRACT: The table grape industry is a major contributor to the South African economy, directly through foreign earnings from this predominantly export-based industry, as well as indirectly through the employment of thousands of people. It is a growing industry and consists of several production areas. The fastest growing table grape production area in South Africa is the Lower Orange River area, which produces some of the earliest grapes in the Southern Hemisphere. The biggest river in South Africa irrigates this area and it has an extreme climate characteristic of semi-desert areas. This area is considered to be optimal for the production of high quality, early, white seedless grapes. Previously, this area was predominantly planted to Sultanina vines for the purpose of raisin production. When seedless table grapes became a consumer preference, the producers very successfully converted their production practices to yield export quality seedless grapes from the established Sultanina vineyards. Extensive new plantings as well as re-plantings occurred in this area, also including newer cultivars from local and overseas breeding programmes. Being a viticultural and economical hot-spot, the Lower Orange River area is attracting much attention as a table grape production area and it also formed the backdrop to this study. The cultivar profile is changing in the area and it is projected that Sultana-, Regal-, Prime Seedless and Sugraone will be the four major early, white seedless cultivars in 2005. Based on this knowledge and prompted by a lack of information regarding production costs and general profitability of the new cultivars, this study was initiated in the form of a comparative analysis between the four mentioned cultivars spanning the early, middle and late regions of the Lower Orange River area. The approach used extracted information regarding cultural input costs (specifically labour as man-hours and the consequent costs) per manipulation performed in the vineyards. This approach is different from the more general method of obtaining input costs for a specific area based on combined mean values, often not distinguishing between cultivars. The specific aims of the study included a comparative analysis of input costs for production cultural practices per main manipulation action, as well as a comparative analysis taking into account productivity, value and extraordinary costs related to each of the four cultivars. To this end, 22 experimental plots were identified for use in the study. Collaboration of the production managers of each of the experimental plots were procured and information regarding production costs per manipulation and productivity of each cultivar and experimental plot were extracted from their own record keeping systems or from documents provided to the production managers. The value (price achieved) of the various cultivars for the 2001/2002 table grape season were put into perspective by using data from a survey which included information regarding payments for the various cultivars during the season in the Lower Orange River area. Information regarding fruit and vine royalties was obtained from the various plant breeders' rights holders of the various cultivars, where applicable. Primary descriptions of each experimental plot concerning general cultivation practices and information regarding the specific season were used to qualify results obtained from the various blocks. Several complicating factors impacted on the study and specifically the subsequent analyses of the results. Some of the factors were already identified as complicating factors in the planning stage of the study and were mostly linked to the recent introduction of two of the cultivars to the Orange River area. From the data gathered and the analyses performed it became clear that it would be difficult to discern significant differences (where significant is defined as PS0.10), but clear trends were observed and indications obtained. Based on the input cost analyses of this study it is proposed that mature Prime Seedless will have the highest labour input and cultural production cost of the four cultivars, followed by Sugraone. The labour input and the cost for the production cultural practices studied for young Prime Seedless vines were very high in comparison to the mature Sultana Seedless and Sugraone vines, especially for the canopy management and bunch manipulation actions. Prime Seedless was especially prone to the set of small and uneven berries, which lead to very high labour input requirements and subsequent cost for bunch manipulations. Sugraone is also known for the set of small and uneven berries in the Lower Orange River area, especially in difficult climatic seasons, also requiring high labour input for bunch manipulations. The initial indication is that mature Sultana- and Regal Seedless will require similar labour inputs for cultural production practices. The fact that Regal Seedless does not require expensive gibberellic acid (GA) applications, or girdling for thinning and berry enlargement purposes, is a tremendous advantage from a production cost point of view. Accordingly, initial indications are that Regal Seedless will have the lowest cultural production cost of the four cultivars. Regal Seedless was prone to the set of uneven berries during the year of study and accordingly it is suspected that this factor will ultimately determine the labour requirements and cultural production input cost, especially in difficult climatic seasons. The labour input and ultimately the cultural production cost for Sultana Seedless will be determined by the correct timing and concentration of the GA applications for thinning and berry sizing. Sultana Seedless and Sugraone produced high yields during the 2001/2002 table grape season in the Lower Orange River area. Yield information from the various experimental plots confirmed that there is little to choose between the two cultivars in terms of yield when cultivation conditions and practices are optimal. Large variation was observed in the yield results from the Regal- and Prime Seedless experimental plots. This is largely due to the recent introduction of the cultivars to the area and the consequent scarcity of blocks of these cultivars that are in full production. It was impossible to identify clear trends in terms of the future productivity of mature Regal- and Prime Seedless, but some indications of labour inputs could be extracted and qualified. Early maturing Prime Seedless and Sugraone performed very well in terms of price, especially in the harvest period prior to week 50. This advantage of high prices early in the season is, however, not always applicable to early cultivars in the later maturing regions of the Lower Orange River area. Later during the season, after week 50, when the supply of table grapes to the overseas markets has increased sharply, Sultana Seedless is usually the best performer in terms of price of the four cultivars. The ultimate price obtained by a cultivar is to a large extent determined by supply and demand, quality and acceptance of the specific cultivar. This study and its outcomes have a strong regional (Lower Orange River) and local (South Africa) impact and the specific results will undoubtedly be valuable to the producers, exporters and other role-players with vested interest in the cultivars studied or in table grape production per se. The methodology adopted in this study, however, is of broader interest and dearly shows the advantage of having detailed and qualified information regarding cultivation practices and bringing it in relation to the labour and consequent costs required per action. This should lead to more business intelligence and realistic planning on the producer side when decisions regarding the choice of a cultivar for a specific production area with a particular marketing scope have to be made. This study has also paved the way for similar studies, specifically with regard to the detailed description of the methodology that was established. Knowledge of the problems experienced in this study provides a useful reference for the planning and execution of similar studies. / AFRIKAANSE OPSOMMING: Die tafeldruifindustrie dra grootskaals by tot die Suid- Afrikaanse ekonomie: regstreeks deur middel van buitelandse valuta vanaf hierdie hoofsaaklik uitvoer-gebaseerde industrie, asook indirek deur werkverskaffing aan duisende mense. Dit is 'n vinnig groeiende industrie en bestaan uit verskeie produksie-areas waarvan die Benede-Oranjerivierarea, waar van die vroegste druiwe in die suidelike halfrond geproduseer word, tans die meeste groei toon. Die grootste rivier in Suid-Afrika vloei deur hierdie gebied wat deur uiterste klimaatstoestande, soortgelyk aan die van semi-woestyngebiede, gekenmerk word. Hierdie gebied is baie gunstig vir die produksie van hoë-gehalte, vroeë, wit pitlose druiwe. In die verlede is hoofsaaklik Sultanina vir die produksie van rosyne in hierdie gebied verbou. Namate pitlose tafeldruiwe voorkeur begin geniet het onder verbruikers wêreldwyd, het produseerders in die area hul verbouingspraktyke suksesvol aangepas vir die produksie van uitvoergehalte tafeldruiwe vanaf die grootskaalse, reeds gevestigde Sultanina-wingerde. Uitgebreide aanplantings en heraanplantings, wat nuwe cultivars van plaaslike en oorsese teelprogramme ingesluit het, is in hierdie gebied gedoen. Die vinnige groei in tafeldruifaanplantings en -uitvoere, asook die ekonomiese impak van die industrie in die Benede-Oranjeriviergebied, het die afgelope aantal jaar sterk op die voorgrond getree en het gevolglik gedien as agtergrond vir hierdie studie. Die cultivarprofiel in dié area is besig om te verander en volgens vooruitskattings gaan Sultana, Regal, Prime Seedless en Sugraone die vier prominente vroeë, wit, pitlose tafeldruifcultivars in 2005 wees. Gebaseer op hierdie feit en na aanleiding van 'n behoefte aan meer inligting met betrekking tot produksiekostes en algemene winsgewendheid van die nuwe cultivars, is 'n vergelykende studie aangaande die vier genoemde cultivars in die Benede-Oranjeriviergebied geloods. Die benadering wat gedurende die studie gevolg is, het inligting aangaande produksie-insetkoste (spesifiek arbeid in man-ure en gevolglike koste) per manipulasie onttrek. Hierdie benadering verskil van die meer algemene metodiek om insetkoste-inligting van 'n spesifieke area van gekombineerde gemiddelde waardes te verkry. Met so 'n benadering word gewoonlik geen onderskeid tussen cultivars getref nie. Die spesifieke doelwitte van hierdie studie het 'n vergelykende analise aangaande die insetkoste van die produksiepraktyke per hoofmanipulasie/aksie ingesluit, asook 'n analise waar produktiwiteit, waarde en buitengewone koste van die vier cultivars in ag geneem is. In totaal is 22 eksperimentele persele gebruik in die studie. Samewerking van die produksiebestuurders van die onderskeie esperimentele persele is verkry ten opsigte van die verskaffing van inligting oor produksiekoste per manipulasie, en die produktiwiteit per cultivar en eksperimentele perseel. Die produksiebestuurders het die nodige dokumente ontvang om die inligting te onttrek, of kon die inligting verskaf soos dit in hul rekordhoudingsisteem voorgekom het. Die waarde (prys behaal) van die onderskeie cultivars vir die 2001/2002-seisoen is in perspektief gestel deur gebruik te maak van 'n opname wat in die Benede Oranjeriviergebied plaasgevind het. Hierdie opname het inligting oor die uitbetalings van die onderskeie cultivars in die area vir die 2001/2002- seisoen ingesluit. Inligting rakende die stok- en vrugproduksie-tantieme is vanaf die onderskeie plantttelersregtehouers van die cultivars verkry. Primêre beskywings van die algemene verbouingspraktyke van elke eksperimentele blok en inligting oor die spesifieke seisoen is gebruik om die data wat vanaf die esperimentele persele verkry is, in perskektief te stel. Verskeie kompliserende faktore het die studie en die ontleding van data beïnvloed. Verskeie van hierdie faktore is reeds geidentifiseer met die beplanning van die studie en was meestal gekoppel aan die onlangse bekendstelling van Regal en Prime Seedless aan die Benede-Oranjeriviergebied. Na aanleiding van die data wat ingesamel en ontleed is, was dit duidelik dat dit moeilik sou wees om betekenisvolle verskille (waar "betekenisvol" as PS0.10 gedifinieer is) tussen die cultivars uit te lig, maar dat dit egter wel moontlik sou wees om aanvanklike indikasies en tendense te kry. Gebaseer op die insetkoste-ontleding van die studie blyk dit dat volwasse Prime Seedless die hoogste arbeidsinsetle en produksiekoste van die vier cultivars gaan hê, gevolg deur Sugraone. Die arbeidsinsetle en koste van die produksie-aksies wat van jong Prime Seedless bestudeer is, was baie hoog in vergelyking met volwasse Sultana Seedless- en Sugraone-stokke, veral ten opsigte van lowerbestuur en trosmanipulasies. Prime Seedless was veral geneig tot die set van klein, oneweredige korrels, wat tot baie hoë arbeidsinsetle en gevolglik koste vir trosmanipulasies gelei het. Sugraone is ook daarvoor bekend dat dit geneig is tot die set van klein, oneweredige korrels in die Benede-Oranjeriviergebied (veral in moeilike klimaatseisoene), wat gevolglik tot hoë arbeidsinstle vir trosmanipulasie lei. Die aanvanklike aanduiding is dat volwasse Sultana en Regal Seedless min of meer die dieselfde arbeidsinsetle vir verbouing sal vereis. Die feit dat Regal Seedless nie duur gibberelliensuur (GS)-behandelings vir blomtrosuitdunning of korrelvergroting benodig nie, is 'n enorme voordeel in terme van produksiekoste. Gevolglik is die aanvanklike aanduiding dat Regal Seedless die laagste produksieskoste van die vier cultivars sal hê. In die studiejaar was Regal Seedless egter geneig tot die set van onweredige korrels en gevolglik word verwag dat hierdie faktor uiteindelik die arbeidsinsetle en produsiekoste van die cultivar sal bepaal, veral in moeilike klimaatseisoene. Die arbiedsinsetle en produksiekoste van Sultana Seedless sal bepaal word deur die korrekte tydsberekening en konsentrasie van die GS-behandelings vir uitdunning en korrelvergroting. Sultana Seedless en Sugraone het gedurende die 2001/2002-seisoen hoë opbrengste in die Benede-Oranjeriviergebied geproduseer. Oesdata inligting van die onderskeie esperimentele persele het bevestig dat daar min te kies is tussen die twee cultivars in terme van produktiwiteit wanneer verbouingstoestande en -praktyke optimaal is. Groot variasie is egter waargeneem in die opbrengsresultate van die Regal en Prime Seedless. Dit is hoofsaaklik as gevolg van die onlangse bekendstelling van die twee cultivars in die area en dus ook die beperkte aantal blokke van die cultivars wat reeds in vol produksie was. Dit was dus onmoontlik om duidelike tendense in terme van die toekomstige produksie van volwasse Regal en Prime Seedless te identifiseer. Indikasies van arbeidsinsette en produksiekoste kon egter wel verkry word. Vroeg rypwordende Prime Seedless en Sugraone vaar baie goed in terme van die prys wat dit behaal, veral in die oesperiode voor week 50. Hierdie voordeel van hoë pryse behaal vroeg in die seisoen is egter nie altyd van toepassing op vroeë cultivars in die later rypwordende areas van die Benede-Oranjeriviergebied nie. Later in die seisoen (na week 50), wanneer die aanbod van tafeldruiwe op oorsese markte skerp toegeneem het, is Sultana Seedless gewoonlik die beste presteerder in terme van prys van die vier cultivars. Die uiteindelike prys wat deur cultivars behaal word, word tot 'n groot mate bepaal deur vraag en aanbod, kwaliteit en aanvaarding van die cultivar deur die verbruiker. Die studie en die uitkomste daarvan het 'n sterk streeks (Benede-Oranjerivier) en plaaslike (Suid-Afrika) impak, en die spesifieke resultate salongetwyfeld van waarde wees vir produseerders, uitvoerders en ander rolspelers met bestaande belange in die cultivars of vir tafeldruifproduksie as sulks. Die metodiek wat in hierdie studie gebruik is, is egter van breêr belang en wys duidelik die voordele daarvan om gedetailleerde en gekwalifiseerde inligting aangaande produksiepraktyke te hê, wat dit ook in verband bring met arbeid en gevolglike koste per aksie. Dit behoort te lei tot meer besigheidsintelligensie en realistiese beplanning deur die produseerder met betrekking tot cultivarkeuse vir 'n spesifieke produksiearea met 'n spesifieke bemarkings geleentheid. Hierdie studie het ook die weg gebaan vir soortgelyke studies, spesifiek ten opsigte van die gedetailleerde beskrywing van die metodiek wat gevestig is. Kennis van die probleme wat in hierdie studie ondervind is, kan dien as nuttige verwysing vir die beplanning en uitvoer van soortgelyke studies.

Thesis (MScAgric)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: Olives have been produced commercially in the Mediterranean-type climate of the Western Cape region of South Africa since the early 1900’s. As in the rest of the world, South African table olive producers struggle with alternate bearing. Naphthalene acetic acid (NAA) has been used since the 1950’s to thin table olives in California. To date, South African producers opted to tolerate rather than try to reduce the negative effects of alternate bearing. However, due to increased olive production, profit margins are decreasing and producers can no longer ignore the negative effects of alternate bearing. Since the efficacy of NAA as a thinning agent is modulated by environmental conditions and genotype, trials were conducted to evaluate the use of NAA on locally important cultivars under South African conditions. The main aim was to establish optimum application rates for ‘Barouni’, ‘Mission’ and ‘Manzanillo’. NAA decreased the fruit number per tree, thereby improving fruit quality (increased fruit size and a higher proportion black fruit in the case of ‘Mission’) in all three cultivars. Thinning did not affect the return bloom in any of the cultivars. In the case of ‘Barouni’, the lack of a return bloom response could be due to the low level of thinning achieved, while climatic conditions during flower development may be to blame for the lack of response in ‘Mission’ and ‘Manzanillo’. Although NAA application did not affect the income per hectare, profitability may increase as harvesting costs account for roughly 50% of the input costs. Based on our results, NAA at 200 mg L-1, applied 10 to 15 days after full bloom, is recommended for local conditions. This concentration is slightly higher than the application rates used in California. An even higher NAA concentration might be used when premium prices are paid for large fruit, as in the case of ‘Mission’ and ‘Manzanillo’. However, NAA at 400 mg L-1 seemed to decrease vegetative growth in ‘Mission’, which may decrease bearing positions for the next season. Earlier application should be considered for a heavy “on” crop while the concentration can be decreased or the spray time delayed to decrease thinning when an average crop is anticipated. Gibberellic acid (GA3) was applied during an “off” season to ‘Mission’ and ‘Manzanillo’ to determine when during the season floral induction is inhibited by the simulated seed produced hormone. GA3 had its greatest effect on the extent of flowering in ‘Manzanillo’ when applied at the time of endocarp sclerification in early December. It follows from this result that to prevent the negative effects of a crop load on flowering in the subsequent season, thinning has to occur before endocarp sclerification. Later application of GA3 in January and February also decreased flowering, but to a lesser extent than application in December. These later applications possibly decreased flower initiation in buds on shoots that continued growing for longer or they may also have interfered with flower differentiation. The effect of the reduced “on” crop in the 2010/2011 season in GA3-treated trees on yield in the 2011/2012 season still needs to be determined. / AFRIKAANSE OPSOMMING: Olywe word sedert die vroeë 1900’s kommersieel geproduseer in die Mediterreense tipe klimaat Wes-Kaap streek van Suid Afrika. Soos in die res van die wêreld, is alternerende drag ‘n reuse struikelblok vir Suid-Afrikaanse olyfprodusente. Anders as in California waar naftaleenasynsuur (NAA) reeds vanaf die 1950’s gebruik word om tafel olywe uit te dun, het Suid-Afrikaanse produsente tot op hede die gevolge van alternerende drag verduur eerder as om die negatiewe effekte daarvan te probeer verminder. Weens ‘n afname in winsgewendheid vanweë ‘n toename in olyfproduksie kan Suid-Afrikaanse olyfprodusente egter nie meer langer die negatiewe effekte van alternerende drag ignoreer nie. Die effektiwiteit van NAA as uitdunmiddel word beïnvloed deur omgewingstoestande asook deur die plant se genetika. Gevolglik is proewe onderneem om die gebruik van NAA te evalueer op plaaslik belangrike kultivars en onder Suid-Afrikaanse kondisies. Die hoofdoel van die proewe was om optimale toediening konsentrasies van NAA vir ‘Barouni’, ‘Mission’ en ‘Manzanillo’ te bepaal. NAA het die vruglading per boom verminder en daardeur vrugkwaliteit (vruggrootte asook ‘n groter proporsie swart vrugte in die geval van ‘Mission’) in al drie kultivars verbeter. In al drie kultivars het uitdunning egter geen effek op die volgende seisoen se blom gehad nie. In die geval van ‘Barouni’ kan die swak opvolgblom moontlik toegeskryf word aan die lae vlak van uitdun terwyl klimaatstoestande tydens blomontwikkeling moontlik die oorsaak was vir die swak reaksie van ‘Mission’ en ‘Manzanillo’. Alhoewel toediening van NAA nie die bruto inkomste per hektaar verhoog het nie, kan winsgewendheid moontlik toeneem aangesien oeskoste ongeveer 50% van insetkostes uitmaak. Gebaseer op die resultate van die studie, word NAA toediening teen 200 mg L-1, 10 tot 15 dae na volblom, aanbeveel vir plaaslike toestande. Hierdie konsentrasie is effens hoër as konsentrasies wat in Kalifornië gebruik word. Selfs hoër NAA konsentrasies kan toegedien word wanneer ’n premium betaal word vir groter vrugte, soos in die geval van ‘Manzanillo’ en ‘Mission’. NAA teen 400 mg L-1 het egter vegetatiewe groei in ‘Mission’ verlaag en dit kan moontlik lei tot ‘n vermindering in draposisies in die volgende seisoen. Vroeër toediening moet oorweeg word wanneer ‘n groot “aan” oes verwag word, terwyl die NAA konsentrasie verminder of toediening uitgestel kan word ten einde uitdunning te verminder indien ‘n gemiddeld oes verwag word . Gibberelliensuur (GA3) is in die “af” seisoen toegedien op ‘Mission’ en ‘Manzanillo’ om vas te stel wanneer gedurende die seisoen saad-geproduseerde hormone blominduksie inhibeer. Die grootste effek op blominduksie van ‘Manzanillo’ is verkry met toediening tydens pitverharding gedurende vroeë Desember. Om die negatiewe effek van ‘n hoë vruglading op die volgende seisoen se blom te voorkom, moet vruguitdunning dus voor pitverharding geskied. Later toediening van GA3 in Januarie en Februarie het ook blom verminder, maar tot ‘n mindere mate as toediening in Desember. Hierdie later toedienings het moontlik blominisiasie van knoppe wat later gevorm het geïnhibeer of kon moontlik blomdifferensiasie negatief beïnvloed het. Die effek van die verlaagde “aan” jaar in die 2010/2011 seisoen in reaksie op GA3 toediening op opbrengs in die 2011/2012 seisoen moet nog bepaal word.

Thesis (MscAgric)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: Alternate bearing is a common phenomenon in most commercial perennial fruit trees. In citrus, the “on” year consists of a heavy crop load with mostly small fruit, often followed by an “off” year with few, large and coarse fruit. Carbohydrates play an important role in affecting alternate bearing, especially during fruit set, but also flowering and fruit maturation, and are essential in maintaining a regular bearing habit. Changes in starch and total sugar accumulation in the leaves of the alternate bearing prone „Nadorcott‟ mandarin were followed over an entire season for both “on” and “off” trees to evaluate the possibility of using carbohydrate levels to predict bearing potential. Starch accumulation followed a distinct pattern with differences between “on” and “off” trees visible in April and May. Starch concentrations in April showed a moderate negative correlation with yield and a moderate positive correlation with return bloom. Rapid starch accumulation started prior to harvest with a peak at the beginning of flowering. Thereafter a sharp decrease in starch levels occurred until after full bloom followed by a steady decrease from physiological fruit drop towards fruit maturity. “On” trees bore 53% more fruit than “off” trees, but the return bloom of “off” trees was 140% more than “on” trees, thus illustrating the negative effect that a large crop has on the next season‟s bloom. It was concluded that for „Nadorcott‟ mandarin, leaf starch concentration in April can be used as an indication of bearing potential the following season. Pruning is a well-established management tool to control alternate bearing. Summer pruned trees had more spring flush vegetative shoots, more nodes per shoot and also more growth per parent shoot overall, compared to unpruned, control trees. Control trees had higher light levels inside the tree compared to summer pruned trees. However, no differences in leaf starch or total sugar levels during April were measured between treatments. Production of new bearing sites should therefore be considered in this experiment. It was concluded that pruning during November followed by early regrowth management gave the best balance between light penetration and production of new bearing units. Pruning in November, rather than during winter, also allowed selective pruning of shoots with or without flowers, depending on whether it was an “on” or an “off” year. When fruit thinning chemicals are applied at the optimum time and concentration, it is an effective way of moderating an alternate bearing cycle. Unfortunately no significant differences were obtained in this experiment even though the thinning treatments did show slightly higher starch levels in April 2012, indicating that the demand for energy was lower in these trees. This response was most likely due to the slightly lower yield and fruit number of the thinning treatments compared to the control. The dichlorprop treatment also showed a higher fruit growth rate, and future research should focus on timing of chemical thinning sprays in late mandarin cultivars / AFRIKAANSE OPSOMMING: Alternerende drag is 'n algemene verskynsel by die meeste meerjarige kommersiële vrugtebome. In die “aan” jaar by sitrus word 'n swaar oeslading gedra wat hoofsaaklik uit klein vrugte bestaan gevolg deur 'n “af” jaar met minder, groter en growwer vrugte. Koolhidrate speel 'n belangrike rol, veral gedurende vrugset, maar ook tydens blomtyd en vrugrypwording, en is noodsaaklik om ‟n reëlmatige drasiklus te verseker. Veranderinge in stysel- en totale suiker akkumulasie in die blare van „Nadorcott‟ mandarynbome, is deur die loop van 'n volle seisoen gevolg op beide “aan” en “af” bome om die moontlikheid te ondersoek dat koolhidraatvlakke gebruik kan word om dragpotensiaal te bepaal. Verskille tussen “aan” en “af” bome was in April en Mei sigbaar. Styselvlakke in April het 'n matige negatiewe korrelasie met drag getoon en 'n matige positiewe korrelasie met die volgende seisoen se blom. Styselvlakke het voor oestyd begin toeneem en aan die begin van blomtyd 'n piek bereik waarna 'n skerp daling voorgekom het tot na volblom. Dit is gevolg deur 'n geleidelike afname vanaf fisiologiese vrugval totdat die vrugte ryp was. “Aan” bome het 53% meer vrugte gedra as “af” bome, maar die volgende seisoen se blom van “af” bome was 140% meer. Dit illustreer die negatiewe effek wat ‟n groot oes op die volgende seisoen se blom het. Die gevolgtrekking is dat styselvlakke in blare gedurende April gebruik kan word as 'n aanduiding van die drag-potensiaal vir die komende seisoen vir „Nadorcott‟ mandarynbome. Snoei is 'n gevestigde manier om alternerende drag te beheer. Bome wat in die somer gesnoei is, het 'n groter aantal vegetatiewe lote in die lente, meer knoppe per loot en ook meer groei op ouer-lote gehad in vergelyking met die kontrole bome wat nie gesnoei is. Kontrole bome het hoër ligvlakke binne-in die boom gehad in vergelyking met die bome wat in die somer gesnoei is. Daar is egter in April geen verskille gemeet in die blare se stysel- en totale suikervlakke tussen behandelings nie. Produksie van nuwe dra-posisies moet dus vir hierdie eksperiment in ag geneem word. Die gevolgtrekking was dat, deur in November te snoei en vroeë bestuur van nuwe groei toe te pas, die beste boomvorm verkry is. Deur in November te snoei eerder as in die winter, kon daar ook selektief gesnoei word aan lote met of sonder blomme, afhangende of dit ‟n “aan” of “af” jaar was. Korrekte chemiese vruguitdunning is een van die mees effektiewe maniere om ‟n alternerende drag-siklus te verminder. Ongelukkig is geen betekenisvolle verskille in hierdie eksperiment verkry nie, ten spyte van die feit dat die uitdunningsbehandelings wel ietwat hoër styselvlakke in April 2012 getoon het. Dit dui daarop dat die behoefte aan energie in hierdie bome laer was. Die reaksie was waarskynlik te wyte aan die effens laer oes en vruggetalle as gevolg van die uitdunningsbehandelings in vergelyking met die kontrole. Die dichlorprop-behandeling het ook ‟n hoërvruggroeitempo gestimuleer. Navorsing in die toekoms behoort te fokus op die tydberekening waarvolgens die chemiese uitdunningsmiddels op laat mandarynkultivars toegedien word.

Thesis (MScAgric)--Stellenbosch University, 2012. / ENGLISH ABSTRACT: Fruit trees are exposed to various factors that can adversely affect the production of quality fruit. These factors can directly affect the fruit and the health of the tree and can be classified according to their biotic or abiotic nature, such as pathogenic attacks and drought stress respectively. One of the cultural practices used commercially to address these stresses is the application of fungicides and bactericides. The fruit production industry is under severe pressure from consumers, retailers and environmentalists, locally and internationally, to reduce chemical applications to fruit and fruit trees. The use of natural plant defence elicitor compounds and nutrients offer a potential alternative to fungicide and bactericide sprays and may also increase fruit quality and size as result of a reduction of plant stress. Trials were conducted to evaluate the efficiency of natural plant defence elicitors i.e. salicylic acid (SA) and flavonoids, in addition to mineral nutrients and bactericide/fungicides, on peach (incidence of Xanthomonas infection), plum (induced drought stress and Mg/Mn deficiencies) and apple (Mg/Mn deficiencies) fruit and trees against specified biotic or abiotic stress factors. Trial on Prunus persica cv. ‘Sandvliet’were conducted over two seasons (2008/2009 and 2011/2012) on a commercial site, Protea Farm, in the Worcester area in the Western Cape Province. During the 2008/2009 season the SA (AlexinTM, AlexiboostTM) containing treatments were applied first at 75% petal drop at concentrations of 125 and 250 ml. 100 L-1. The copper (StCu, Cu)-containing treatment was applied at 50% petal drop, while dichlorophen (XanbacTM) treatments were applied at fruit set at concentrations of 150, 300 and 200 ml. 100 L-1. The flavonoid (CroplifeTM) treatment was applied at the start of petal drop at a concentration of 150 ml.100 L-1. During the 2011/2012 season, a new flavonoid (CropbiolifeTM) treatment, as well as potassium (K-MaxTM) treatment, were incorporated into the trial and applied at concentrations of 150 and 500 ml. 100 L-1 respectively. Additionally a SA (AlexinTM) and dichlorophen (XanbacTM) treatments that performed well during the first season, were incorporated into the second season with application times and rates similar to the first season’s protocol. In addition to fruit size and quality measurements, the percentage Xanthomonas infection was determined on the leaves and fruit of the experimental trees. The SA (AlexinTM) containing treatment significantly reduced the incidence of Xanthomonas infection on leaves and fruit compared to the control in the first season. However, results varied between the two seasons, as no significant difference from the control could be obtained in the following season. The AlexinTM treatments also significantly increased the fruit size and quality. The flavonoid (CropbiolifeTM) and K (K-MaxTM) containing treatments similarly reduced the Xanthomonas infection on leaves and fruit, as well as increasing the fruit size and quality in the second season. The dichlorophen (XanbacTM) containing treatment recorded varying results as it significantly reduced the Xanthomonas infection on the fruit only in the second season. The plum trials were conducted over the 2011/2012 season on ‘Laetitia’ and ‘Songold’ plum trees, Welgevallen Experimental Farm, Stellenbosch University. Three SA (AlexinTM, AlexSal and RezistTM) containing foliar treatments were applied on the ‘Laetitia’ trees. Only two SA (AlexinTM, AlexSal) containing foliar treatments were applied on the ‘Songold’ trees. Additionally, a foliar treatment containing only K, Ca, Mg and B, was applied in both the ‘Laetitia’ and ‘Songold’ trials. All the treatments were first applied at 75% petal drop, at the same concentration of 250 ml. 100 L-1. Additionally to fruit size and quality, the mineral nutrient content of the leaves and fruit was determined. The ascorbic acid and glutathione content was determined in fruit at harvest and again after storage. None of the treatments had a positive effect on the parameters measured, except the SA (AlexinTM) containing treatments which increased the titratible acidity (TA) in both at harvest and after storage. The treatments also did not alleviate the induced stress compared to the control. The apple and plum tree trials were conducted over the 2011/2012 season in a semi-closed greenhouse, at the Welgevallen Experimental Farm, Stellenbosch. Magnesium (Mg) and Manganese (Mn) deficiencies were induced in one-year-old ‘Royal Beaut’ apple and ‘Laetitia’ plum trees planted in 10 L nursery bags, by omitting these nutrients from a standard Long Ashton soil application. Foliar treatments of Mg (MagMaxTM) and Mn (ManMaxTM) containing sprays were subsequently applied at concentrations of 250 and 75 ml. 100 L-1 respectively, after deficiency symptoms for these nutrients were visually observed. Mineral nutrient analysis of the leaves were analysed on the 13th of February for the plums and 30th of March 2012, for the apples. The Mn (ManMaxTM) containing treatment successfully overcame the Mn induced deficiency. The Mg (MagMaxTM) containing treatment did not overcome the induced Mg deficiency and was probably due to the deficient nitrogen levels in the plants, caused by an error in the initial Long Ashton nutrient solution formulation. In conclusion AlexinTM, K-MaxTM and CropbiolifeTM have shown their ability to decrease Xanthomonas infection in peaches. Additionally to their positive effect on fruit size and quality on the peaches. SA was not able to overcome the induced stress on plums, but had a positive effect on the fruit quality and size. The ManMaxTM been proven to overcome the induced Mn deficiency, while MagMaxTM was unsuccessful to overcome the Mg deficiency. / AFRIKAANSE OPSOMMING: Vrugtebome word blootgestel aan verskeie faktore wat die produksie van kwaliteit vrugte nadelig kan beïnvloed. Hierdie faktore kan ‘n direkte invloed hê op die vrugte en op die gesondheid van die boom en kan geklassifiseer word op grond van hulle biotiese of abiotiese natuur, soos patogeen infeksie en droogte stres onderskeidelik. Van die produksie praktyke wat gebruik word sluit in die toepassing van verskillende swamdoders en bakterisiede. Die vrugtebedryf is onder geweldige druk van verbruikers, die kleinhandel en omgewingsbewustes om die toediening van chemikalieë aan vrugte en vrugtebome te verminder. Die gebruik van natuurlike plant verdediging stimulerende verbindings en nutriënte, bied 'n moontlike alternatief tot die spuit van swamdoders en bakterisiede, en kan ook moontlik ʼn bydrae maak tot verbeterde vrugkwaliteit en -grootte. Proewe is uitgevoer om die effektiwiteit van die natuurlike plant verdediging stimulante, salisielsuur (SA) en flavonoïede, addisioneel tot verskillende voedingstowwe en bakterieële / swamdoders op perske, pruim en appels teen Xanthomonas infeksie, droogte stres en Mg / Mn tekorte as biotiese en abiotiese stres faktore onderskeidelik te evalueer. Die Prunus persica ‘Sandvliet’ proewe is oor twee seisoene (2008/2009 en 2011/2012) op 'n kommersiële perseel, Protea Farm, in die Worcester-area in die Wes-Kaap Provinsie, uitgevoer. Gedurende die 2008/2009 seisoen is die SA (AlexinTM, AlexiboostTM) bevattende behandelings eers toegedien by 75% blomblaarval teen konsentrasies 125 en 250 ml. 100 L-1. Die koper (StCu, Cu) bevattende behandeling is toegedien by 50% blomblaarval, terwyl die dichlorofen (XanbacTM) bevattende behandelings toegedien is by vrugset, teen konsentrasies van 150, 300 en 200 ml. 100 L-1. Die flavonoïde (CroplifeTM) behandeling is toegedien by die begin van blomblaarval teen 'n konsentrasie van 150 ml. 100 L-1. Gedurende die 2011/2012 seisoen was 'n nuwe flavonoïd (CropbiolifeTM) en ‘n kalium (K-MaxTM) behandeling toegevoeg tot die eksperiment, met ʼn toediening teen konsentrasies van onderskeidelik 150 en 500 ml. 100 L-1. Daarbenewens is die SA (AlexinTM) en dichlorofen (XanbacTM) behandeling van die 2008/2009 seisoen herhaal teen dieselfde konsentrasies en toedieningstye soos in die protokol van die eerste seisoen. Behalwe vir die bepaling van vruggrootte en –kwaliteit, is die persentasie Xanthomonas infeksie op blare en vrugte ook bepaal. Die SA (AlexinTM) bevattende behandeling het die voorkoms van Xanthomonas infeksie op die blare en vrugte betekenisvol verminder in vergelyking met die kontrole. Resultate het egter gewissel in die daaropvolgende seisoen en geen beduidende verskille tussen die behandelings is waargeneem nie. Hierdie SA-bevattende behandelings het ook tot ‘n toename in vruggrootte en -kwaliteit gelei. Die flavonoïde bevattende behandelings, (CropbiolifeTM) en K (K-MaxTM), het soortgelyke afnames in Xanthomonas infeksie op die blare en vrugte in die tweede seisoen getoon, sowel as ‘n toename in vruggrootte en -kwaliteit. Die dichlorofen (XanbacTM) bevattende behandeling het variërende resultate getoon aangesien dit slegs tot ‘n beduidende afname in Xanthomonas infeksie op die blare en vrugte in die tweede seisoen kon lei. Pruim proewe is uitgevoer in die 2011/2012 seisoen op ‘Laetitia’ en ‘Songold’ pruimbome te Welgevallen Proefplaas, Universiteit van Stellenbosch. Drie SA (AlexinTM, AlexSal en RezistTM) bevattende blaar behandelings is toegedien op die ‘Laetitia’ bome. Slegs twee SA (AlexinTM, AlexSal) blaar behandelinge is toegedien op die ‘Songold’ bome. ʼn Verdere K, Ca, Mg en B blaar behandeling is ook toegedien in beide die ‘Laetitia’ en ‘Songold’ proewe. Al die behandelings se eerste toediening het saamgeval met 75% blomblaarval, teen dieselfde konsentrasie van 250 ml. 100 L-1. Addisioneel tot vruggrootte en –kwaliteit, is die mineraal element inhoud van die blare en vrugte bepaal. Die askorbiensuur en glutatioon inhoud is bepaal in die vrugte met oes asook na opberging. Geen behandeling het 'n positiewe uitwerking op die parameters wat gemeet is getoon nie, behalwe een van die SA (AlexinTM) bevattende behandelings wat die titreerbare sure (TS) verhoog het in beide kultivars. Die behandelings kon ook nie die geïnduseerde stres verlig in vergelyking met die kontrole nie. Die appel- en pruim proewe is uitgevoer gedurende die 2011/2012 seisoen in 'n semi-geslote glashuis te Welgevallen Proefplaas, Universiteit van Stellenbosch. Magnesium (Mg) en Mangaan (Mn) tekorte is geïnduseer in een-jaar-oue ‘Royal Beaut’ appel en ‘Laetitia’ pruim bome, aangeplant in 10L kwekerysakke, deur dié elemente uit ʼn toediening van standaard Long-Ashton voedingsoplossing aan die grond weg te laat. Mg (MagMaxTM) en Mn (ManMaxTM) bevattende blaarspuite is daarna toegepas teen onderskeidelik konsentrasies van 250 en 75 ml. 100 L-1. ʼn Minerale analise van die blare is uitgevoer op 13 Februarie, op die pruime en 30 Maart 2012, op die appels. Die Mn (ManMaxTM) bevattend behandeling het die Mn-geïnduseerde tekort verlig. Die Mg (MagMaxTM) bevattende behandeling het nie die geïnduseerde Mg-tekort verlig nie. Dit is moontlik toe te skryf aan die stikstof tekort in die plante wat te wyte was aan ʼn foutiewe Long Ashton voedingsoplossing formulasie wat aanvanklik toegedien is. Ten slotte het AlexinTM, K-MaxTM en CropbiolifeTM getoon dat hul die vermoë het om Xanthomonas infeksie te verminder, asook om vruggrootte en kwaliteit in perskes te verbeter. SA was nie in staat om die geïnduseerde stres op pruime te oorkom nie, maar het 'n positiewe uitwerking op die vruggroote en kwaliteit gehad. ManMaxTM het getoon dat dit ʼn geïnduseerde, visuele Mn tekort kan oorkom, terwyl MagMaxTM onsuksesvol was om die Mg-tekort te oorkom