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1	The effects of various combinations of different classes of anticancer drugs and tyrosine kinase inhibitors on the human MCF-7 breast carcinoma cell line Abrahams, Beynon January 2014 (has links) Magister Scientiae (Medical Bioscience) - MSc(MBS) / This study investigated the effects of TKIs on the growth and proliferation of MCF-7 breast carcinoma cells in culture. MCF-7 cells were exposed to different concentrations of TKIs alone and in combination with each other. Inhibition of cell growth by TKIs used individually occurred in a dose- and time-dependent manner. When EGFR Inhibitor I, EGFR Inhibitor II/BIBX1382 and the multi-specific EGFR/ErbB-2/ErB-4 Inhibitor were used in combination with each other at equimolar log dose concentrations, the combined effects on cell growth was significantly different to inhibitors used individually as reflected in a decreased EC50 (IC50) during combination treatments. Generally, for the combinations with DOX, CPL and the TKIs, synergistic as well as antagonistic effects were observed at isoeffective concentrations with resultant decreases in dose reduction indices (DRIs) implying greater efficacies with the respective combinations. In this study, conventional PCR was used to detect and illustrate the presence of the EGFR gene in the samples, while RT-qPCR was used to determine the mRNA expression levels of this gene in MCF-7 breast carcinoma cells Breast cancer Human MCF-7 breast carcinoma cells Epidermal growth factor receptor Tyrosine kinase inhibitors Doxorubicin Cisplatin EGFR inhibitor I EGFR inhibitor II/BIBX1382 EGFR/ErbB2/ErbB4 inhibitor Dose-response curves IC50/EC50 Drug combination analysis Synergism Antagonism Dose-reduction indices Haematoxylin and eosin staining Annexin V-Cy3 fluorescent staining Apoptosis EGFR gene expression analysis Real-time qPCR

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The effects of various combinations of different classes of anticancer drugs and tyrosine kinase inhibitors on the human MCF-7 breast carcinoma cell line