A Computed Tomographic Imaging Spectrometer (CTIS) is an imaging spectrometer which can acquire a hyper-spectral data set in a single snapshot (one focal plane array integration time) with no moving parts. A specially designed dispersing element, which separates light from the three-dimensional object cube into a grid of two-dimensional prismatic diffraction orders, is the key element in the instrument. The capabilities of the CTIS instrument can be improved by employing a more optimized grating design.There were two main goals to this research: (1) to design a novel CTIS disperser that will improve CTIS capabilities over the previous 5x5 disperser and (2) to integrate the new disperser into the CTIS and evaluate its performance compared to the 5x5 disperser. Six new disperser ideas were evaluated based on their performance in a number of computer simulations to determine the most optimal dispersion pattern. A new CTIS disperser incorporating a novel radial design pattern was developed and tested. Reconstruction results of various spatial and spectral targets are presented. Capabilities of the new CTIS instrument incorporating the radial grating are compared to the previous instrument employing a 5x5 disperser. While both dispersers perform similarly for point-source objects, the radial grating performs better than the previous disperser for extended sources.

Knowledge of water depth is a crucial for planning military amphibious operations. Bathymetry from remote sensing with multispectral or hyperspectral imagery provides an opportunity to acquire water depth data faster than traditional hydrographic survey methods without the need to deploy a hydrographic survey vessel. It also provides a means of collecting bathymetric data covertly. This research explores two techniques for deriving bathymetry and assesses them for use by those involved in providing support to military operations. To support this aim a fieldwork campaign was undertaken in May, 2000, in northern Queensland. The fieldwork collected various inherent and apparent water optical properties and was concurrent with airborne hyperspectral imagery collection, space-based multispectral imagery collection and a hydrographic survey. The water optical properties were used to characterise the water and to understand how they affect deriving bathymetry from imagery. The hydrographic data was used to assess the performance of the bathymetric techniques. Two methods for deriving bathymetry were trialled. One uses a ratio of subsurface irradiance reflectance at two wavelengths and then tunes the result with known water depths. The other inverts the radiative transfer equation utilising the optical properties of the water to derive water depth. Both techniques derived water depth down to approximately six to seven metres. At that point the Cowley Beach waters became optically deep. Sensitivity analysis of the inversion method found that it was most sensitive to errors in vertical attenuation Kd and to errors in transforming the imagery into subsurface irradiance reflectance, R(0-) units. Both techniques require a priori knowledge to derive depth and a more sophisticated approach would be required to determine water depth without prior knowledge of the area of interest. This research demonstrates that water depth can be accurately mapped with optical techniques in less than ideal optical conditions. It also demonstrates that the collection of inherent and apparent optical properties is important for validating remotely sensed imagery.

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Les propriétés optiques de nanotubes de carbone sont décrites idéalement parla physique d’un objet unidimensionnel, donnant lieu notamment à l’apparition des excitons pour décrire les transitions optiques de ces objets. Les expériences d’optique(émission, absorption) réalisées sur ces objets à température ambiante et sur des ensemble d’objets ont permis de confirmer les prédictions théoriques basées sur la physique des objets 1D. Mais à température cryogénique et à l’échelle de l’objet unique,les propriétés optiques observées expérimentalement sont systématiquement très éloignées de celles d’un objet 1D. On peut notamment citer l’apparition de propriétés comme l’émission de photons uniques, qui a largement contribué à l’intensification de la recherche sur ces objets pour des applications en photonique quantique. Ces propriétés sont attribuées à la localisation des excitons le long de l’axe des nanotubes dans des puits de potentiel créés aléatoirement par l’interaction des nanotubes avec leur environnement. Les propriétés optiques sont alors proches de celles des objets0D, et sont fortement modulées par l’environnement. Les mécanismes et l’origine de la localisation et la connaissance physique de ces puits sont encore très limités. Ce travail montre d’une part le développement d’une technique d’absorption sur objet individuel et la caractérisation de sa sensibilité, et d’autre part l’étude statistique de l’émission de nanotubes à température cryogénique. Les résultats obtenus par une technique de super-résolution couplée à une imagerie hyper-spectrale montrent les grandeurs caractéristiques des puits de potentiels au sein de nanotubes individuels.Un dispositif expérimental de photoluminescence résolue en excitation implémenté au cours de ce travail a également montré une modification de l’état excitonique fondamental par l’environnement, avec l’apparition d’une discrétisation spatiale et spectrale de l’état fondamental délocalisé en une multitude d’états localisés. / The optical properties of carbon nanotubes are ideally described by the physicsof a one-dimensional object, giving rise in particular to the emergence of excitons todescribe the optical transitions of these objects. The optical experiments (emission,absorption) carried out on these objects at ambient temperature and on ensemblesconfirm the theoretical predictions based on the physics of 1D objects. But atcryogenic temperature and at the single emitter scale, the optical properties observedexperimentally are systematically different from those of a 1D object. One can citethe emergence of properties such as photon antibunching, which largely contributed tothe intensification of research on these objects for applications in quantum photonics.These properties are attributed to the localization of excitons along the nanotube axisin local potential wells (traps) created randomly by the interaction of nanotubes withtheir environment. The optical properties are then close to those of 0D objects, andare strongly modulated by the environment. The mechanisms and the origin of thelocalization and the physical knowledge of these traps are still very limited. This workshows on the one hand the development of an absorption setup on individual objectand the characterization of its sensitivity, and on the other hand the statistical studyof the emission of nanotubes at cryogenic temperature in a micro-photoluminescencesetup. The results obtained in the later setup by a super-resolution technique coupledwith hyper-spectral imaging show the characteristic quantities of potential wellswithin individual nanotubes. An experimental excitation-resolved photoluminescencesetup implemented during this work also showed a modification of the fundamentalexcitonic state by the environment, with the emergence of a spatial and spectraldiscretization of the delocalized ground state in a multitude of localized states.

The need for water conservation continues to increase as global freshwater resources dwindle. In response, many golf course superintendents are implementing new methods and tools to become more frugal with their water applications. For example, scheduling irrigation using time-domain reflectometer (TDR) soil moisture sensors can decrease water usage. Still, TDR measurements are time-consuming and only cover small scales, leading to many locations being unsampled. Remotely sensed data such as the normalized difference vegetation index (NDVI) offer the potential of estimating moisture stress across larger scales; however, NDVI measurements are influenced by numerous stressors beyond moisture availability, thus limiting its reliability for irrigation decisions. An alternative vegetation index, the water band index (WBI), is primarily influenced by water absorption within a narrow spectral range of near-infrared light. Previous research has established strong relationships between moisture stress of creeping bentgrass (CBG) grown on sand-based root zones, a typical scenario for golf course putting greens. However, this relationship characterizes only a small portion of total acreage across golf courses, which limits widespread adoption. In our research, '007' CBG and 'Latitude 36'hybrid bermudagrass (HBG) were grown on three soil textures, USGA 90:10 sand (S), sand loam (SL) and clay (C), arranged in a 2 x 3 factorial design, randomized within six individual dry-down cycles serving as replications. Canopy reflectance and volumetric water content (VWC) data were collected hourly between 0700 and 1900 hr using a hyperspectral radiometer and an embedded soil moisture sensor, until complete turf necrosis. The WBI had the strongest relationship to VWC (r = 0.62) and visual estimations of wilt (r = -0.91) compared to the green-to-red ratio index (GRI) or NDVI. Parameters associated with non-linear regression were analyzed to compare grasses, soils, indices, and their interactions. The WBI and GRI compared favorably with each other and indicated significant moisture stress approximately 28 hr earlier than NDVI (P = 0.0010). WBI and GRI respectively predicted moisture stress 12 to 9 hr before visual estimation of 50% wilt, whereas NDVI provided 2 hr of prediction time (P = 0.0317). When considering the time to significant moisture stress, the HBG lasted 28 hr longer than CBG, while S lasted 42 hr longer than either SL and C (P ≤ 0.0011). Nonlinear regression analysis showed that WBI and GRI can be useful for predicting moisture stress of CBG and HBG grown on three diverse soils in a highly controlled environment. Our results provide substantial evidence and direction for future research investigating how WBI and GRI can expedite moisture stress assessment and prediction on a large-acreage basis. / Master of Science in Life Sciences / Managed turfgrasses provide several benefits including filtering pollutants, cooling their surroundings, generating oxygen, preventing erosion, serving as recreational surfaces, and increasing landscape aesthetics. Intensively managed turfgrass systems, such as on golf courses and sports fields, require more inputs to maintain acceptable conditions. Freshwater use is often excessive on intensively managed turfgrasses to maintain proper plant growth. Drought conditions often limit water availability, especially in regions with limited rainfall. Turf managers tend to over-apply water across large acreage when few localized areas begin to show symptoms of drought. Additionally, turf managers sometimes wrongly identify stressed areas from other factors as ones being moisture-deprived. Advancements such as the use of soil moisture meters have simplified irrigation decisions as an aid to visual inspections for drought stress. While this method enhances detection accuracy, it still provides no solution to increase efficiency. Expanding our current knowledge of turfgrass canopy light reflectance for rapid moisture stress identification can potentially save both time and water resources. The objective of this research was to enhance our ability to identify and predict moisture stress of creeping bentgrass (CBG) and hybrid bermudagrass (HBG) canopies integrated into varying soil textures (USGA 90:10 sand (S), sand loam (SL) and Clay (C)) using light reflectance measurements. Dry-down cycles were conducted under greenhouses conditions collecting soil moisture and light reflectance data every hour from 7 am to 7 pm after saturating and withholding water from established plugs. Moisture stress was most accurately estimated over time using two vegetation indices, the water band index (WBI) and green-to-red ratio index (GRI), with approximately ninety percent accuracy to visible wilt stress. The WBI and GRI predicted moisture stress of CBG in all soil types and HBG in SL and C approximately 14 hours before the grasses reached 50% wilt. While light reflectance varies on exposed soils, our research shows that underlying soils do not interfere with measurements across typical turfgrass stands. This research provides a foundation for future research implementing rapid, aerial measurements of moisture stressed turfgrasses on a broad application of CBG and HBG on constructed or native soils.

AbstractIn the field of Biomedical Optics measurement of tissue optical properties, like absorption, scattering, and reduced scattering coefficient, has gained importance for therapeutic and diagnostic applications. Accuracy in determining the optical properties is of vital importance to quantitatively determine chromophores in tissue.There are different techniques used to quantify tissue chromophores. Reflectance spectroscopy is one of the most common methods to rapidly and accurately characterize the blood amount and oxygen saturation in the microcirculation. With a hyper spectral imaging (HSI) device it is possible to capture images with spectral information that depends both on tissue absorption and scattering. To analyze this data software that accounts for both absorption and scattering event needs to be developed.In this thesis work an HSI algorithm, capable of assessing tissue oxygenation while accounting for both tissue absorption and scattering, is developed. The complete imaging system comprises: a light source, a liquid crystal tunable filter (LCTF), a camera lens, a CCD camera, control units and power supply for light source and filter, and a computer.This work also presents a Graphic processing Unit (GPU) implementation of the developed HSI algorithm, which is found computationally demanding. It is found that the GPU implementation outperforms the Matlab “lsqnonneg” function by the order of 5-7X.At the end, the HSI system and the developed algorithm is evaluated in two experiments. In the first experiment the concentration of chromophores is assessed while occluding the finger tip. In the second experiment the skin is provoked by UV light while checking for Erythema development by analyzing the oxyhemoglobin image at different point of time. In this experiment the melanin concentration change is also checked at different point of time from exposure.It is found that the result matches the theory in the time dependent change of oxyhemoglobin and deoxyhemoglobin. However, the result of melanin does not correspond to the theoretically expected result.