The following qualitative research explores the adoption of Zero Trust in organisations from an organisational and user acceptance perspective. From an organisational perspective, the research highlights essential aspects such as testing the Zero Trust architecture in a pre-adoption phase, involving top management in the planning phase, communicating in a non-technical language, and making end-users feel a personal connection to IS security. The research highlights the importance of balancing the ease of use with security, evaluating the end-user's technical maturity, and carrying out evaluations from a user acceptance perspective. To gather valuable empirical data, the researcher has conducted semi-structured interviews with highly competent respondents within the field of Zero Trust. Most of the literature available today within Zero Trust focuses on technical aspects, and this research is a unique and vital contribution to the limited available research.