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The Building Breathes Together presents a realm of speculative, industrial habitation and alchemical production. In my instillation, I look to raise questions surrounding romantic notions of production and utility. The work introduces a surreal and haunted space of decomposition and regeneration.

Thesis (Ph. D.)--University of New South Wales, 2002. / Also available online.

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In recent years, several industrial yeasts, owing to their robust growth and certain other characteristics, have been developed as recombinant host systems for commercial production of heterologous proteins. One such yeast Pichia pastoris has proven to be an excellent host for production of secreted and intracellular proteins (Cereghino and Cregg. 2000). The increasing popularity of this particular expression system can be attributed to several factors, most importantly: (1) the simplicity of techniques needed for the molecular genetic manipulation of Pichia pastoris and their similarity to those of Saccharomyces cerevisiae, one of the most well-characterized experimental systems in modern biology;(2) the ability of Pichia pastoris to produce foreign proteins at high levels, either intracellularly or extracellularly; and (3) the capability of performing many eukaryotic post-translational modifications, such as glycosylation, disulfide bond formation and proteolytic processing. The expression level for a given recombinant protein produced by Pichia pastoris seems to be determined largely by its inherent properties such as amino acid sequence, the tertiary structure and the site for expression (Sreekrishna et al.,1997). The attempts on increasing the protein expression levels by far are focused on genetic manipulations to enhance the gene expression and protein stability. Although this is crucial, there is ample scope to improve the productivity of Pichia pastoris fermentations by undertaking a systematic program of optimizing the entire fermentation process. This work aims at undertaking such a program by focusing on strategy to identify and to characterize trends in the behavior of the system. It can be expected that by addressing the process as a whole, rather than narrowly focusing on the protein expression alone, the methodology proposed here can simplify process scale-up and can be applied to several products made by the same host. Pichia pastoris is methylotrophic yeast. In the Pichia pastoris fermentation, the limiting carbon source is glycerol, method or mixture of both. It can grow on methanol as a sole carbon and energy source. It possesses a highly inducible methanol utilization pathway. The first step in the metabolism of methanol is the oxidation of methanol to formaldehyde using molecular oxygen by alcohol oxidase (AOX). AOX, the first enzyme of the pathway, accounts for up to 35% of the total protein in cells grown on limited amounts of methanol. The enzymes undetectable in cells grown on glucose, ethanol or glycerol. There are two genes in Pichia pastoris that code for AOX: AOXI. The AOXI gene product accounts for the majority of alcohol oxidase activity in the cell. This highly inducible and stringently regulated AOXI promoter has been used to construct expression vectors for the production of heterologous proteins in Pichia pastoris. Although some foreign proteins have expressed well in shake-flask cultures, expression levels are typically low compared to fomenter cultures. There are several key aspects of Pichia pastoris fermentations: 1. Fed-batch operation – Controlled addition of glycerol, methanol or mixture thereof. In general, strains are grown initially in a defined medium containing glycerol as its carbon source (growth phase). During this phase, biomass accumulates but heterogonous gene expression is fully repressed. Upon depletion of glycerol, a transition phase is initiated in which additional glycerol is fed to the culture at a growth-limiting rate. Finally, method a mixture of glycerol and methanol is fed to the culture to induce expression (induction phase). The duration of individual substrate feeds, the amount and mode of feeding are critical to optimal fermentation performance. 2.Online measurement and control-One of the most important key parameters in Pichia pastor is expression system is the methanol concentration. Monitoring and controlling this variable are important because high levels of this inductor substrate can be toxic to the cells and low levels of methanol may not be enough to initiate the AOX transcription (Cereghino and Cregg, 2000) This research work aims at investigation the above mentioned aspects by conduction an in depth engineering analysis of the Pichia Pastoris fermentations.

Este trabalho relata uma investigação realizada junto a uma empresa montadora de linha branca com o objetivo de identificar os assuntos de Física escolar presentes em sua linha de produção. Tratando-se de uma pesquisa realizada na fronteira entre dois campos, ao mesmo tempo que se apoiou em referenciais teóricos relativos à Educação e Trabalho, também necessitou de elementos pertinentes ao campo específico do Ensino de Física. Tomando como referência, num primeiro momento, a história da educação, principalmente no que concerne à sua relação com a produção industrial, e partindo do pressuposto de que o conhecimento presente no processo produtivo pode interagir com o conhecimento escolar, sem que para isso se estabeleça uma relação de dependência entre a indústria e a escola, a pesquisa se desenvolveu como um estudo de caso e, por meio de visitas ao setor produtivo da Empresa, entrevistas com alguns de seus funcionários e análise de questionários por eles respondidos, conseguiu-se identificar os assuntos escolares de Física presentes no processo produtivo investigado. Entre outros aspectos, discutiu-se sobre os espaços em que ocorre a circulação desses conhecimentos, ressaltando-se tanto a escola quanto a fábrica, na qual assumiram especial papel os próprios colegas de serviço, os supervisores e os assim denominados \"padrinhos\", funcionários com maior experiência que transmitem aos recém admitidos os conhecimentos necessários para um bom desempenho profissional. Procurou-se também identificar o significado que o conhecimento de Física tem, tanto para o ingresso como para a manutenção no emprego, assim como para a operação de equipamentos com alta tecnologia incorporada. Finalmente, procurou-se ressaltar as possíveis aproximações entre o conhecimento escolar de Física e o conhecimento presente na produção da empresa investigada, além de serem também tecidas considerações a respeito das possíveis implicações dos estudos realizados. / This work relates the investigation done between a company that produces appliances with the objective to identify the School Physics issues in the production line. Being a research done on the border of two fields, as well as theoretical references concerned to Education and Work, it used some pertinent elements specifically to Physics Education. Considering as reference the history of education, specially on what concerns to its relation with the industrial production and assuming that the actual knowledge in the productive process can interact with the school knowledge, although there will be no need for a relation of dependence between industry and school, the research developed as a case study, and through some interviews with employees, visits to the shield as well as analysis of the inquiries they had answered, had the possibility to identify the subjects related to academic Physics enclosed on the investigated work environment. Among other subjects, was discussed the spaces on which arrives the exchange of the knowledge emphasizing either the school and the company, on which we had the special contribution of the workers, the supervisors and the \"godfathers, employees with larger experience who transmit to the just admitted the necessary knowledge for a better professional performance. Tried either to identify the importance that the knowledge of Physics has not only to get a work position but also to keep that position as well as the operation of high tech equipment. Finally it tried to reveal the implications between the Physics knowledge from school and the Physics knowledge at the company that we analyzed and some considerations were made about possible implications of the done researches.

Este trabalho relata uma investigação realizada junto a uma empresa montadora de linha branca com o objetivo de identificar os assuntos de Física escolar presentes em sua linha de produção. Tratando-se de uma pesquisa realizada na fronteira entre dois campos, ao mesmo tempo que se apoiou em referenciais teóricos relativos à Educação e Trabalho, também necessitou de elementos pertinentes ao campo específico do Ensino de Física. Tomando como referência, num primeiro momento, a história da educação, principalmente no que concerne à sua relação com a produção industrial, e partindo do pressuposto de que o conhecimento presente no processo produtivo pode interagir com o conhecimento escolar, sem que para isso se estabeleça uma relação de dependência entre a indústria e a escola, a pesquisa se desenvolveu como um estudo de caso e, por meio de visitas ao setor produtivo da Empresa, entrevistas com alguns de seus funcionários e análise de questionários por eles respondidos, conseguiu-se identificar os assuntos escolares de Física presentes no processo produtivo investigado. Entre outros aspectos, discutiu-se sobre os espaços em que ocorre a circulação desses conhecimentos, ressaltando-se tanto a escola quanto a fábrica, na qual assumiram especial papel os próprios colegas de serviço, os supervisores e os assim denominados \"padrinhos\", funcionários com maior experiência que transmitem aos recém admitidos os conhecimentos necessários para um bom desempenho profissional. Procurou-se também identificar o significado que o conhecimento de Física tem, tanto para o ingresso como para a manutenção no emprego, assim como para a operação de equipamentos com alta tecnologia incorporada. Finalmente, procurou-se ressaltar as possíveis aproximações entre o conhecimento escolar de Física e o conhecimento presente na produção da empresa investigada, além de serem também tecidas considerações a respeito das possíveis implicações dos estudos realizados. / This work relates the investigation done between a company that produces appliances with the objective to identify the School Physics issues in the production line. Being a research done on the border of two fields, as well as theoretical references concerned to Education and Work, it used some pertinent elements specifically to Physics Education. Considering as reference the history of education, specially on what concerns to its relation with the industrial production and assuming that the actual knowledge in the productive process can interact with the school knowledge, although there will be no need for a relation of dependence between industry and school, the research developed as a case study, and through some interviews with employees, visits to the shield as well as analysis of the inquiries they had answered, had the possibility to identify the subjects related to academic Physics enclosed on the investigated work environment. Among other subjects, was discussed the spaces on which arrives the exchange of the knowledge emphasizing either the school and the company, on which we had the special contribution of the workers, the supervisors and the \"godfathers, employees with larger experience who transmit to the just admitted the necessary knowledge for a better professional performance. Tried either to identify the importance that the knowledge of Physics has not only to get a work position but also to keep that position as well as the operation of high tech equipment. Finally it tried to reveal the implications between the Physics knowledge from school and the Physics knowledge at the company that we analyzed and some considerations were made about possible implications of the done researches.

This master thesis is dedicated to Russian development and determinants of its growth. Just two decades ago Russia experienced a total change in the political and economic structure of the country. USSR fall, together will all its successes and failures. Russia went to the path of building a market economy, while the rest of the developed world was already living in such economy and using its benefits. With the heritage of the USSR, Russia had a huge potential to be successful in that road. The goal of this work is to answer the question, whether or not we were able to use it. In order to answer this question two-stage analysis was performed, combining theoretical and quantitative parts. Theoretical part contains the overall analysis of the areas of economic and social development. Quantitative part contains analysis of the main GDP drivers and endogenous powers of industrial production. Powered by TCPDF (www.tcpdf.org)