Master of Science / Department of Animal Sciences and Industry / James M. Lattimer / A study was conducted in a Latin rectangle to determine the impact of stressors on immune function in yearling horses. After 51 d of supplementation with either block 1, a molasses block containing 14.30% fat or block 2, a molasses block containing 7.58% fat, horses were subjected to three forms of stress. Blood samples were collected 6 h pre-stress and at h 1, 6, 12, 24, 48, 72, 96, and 168 post stress. Nasopharyngeal flush (NPF) was conducted 6 h pre-stressor and h 12 and 72 post-stressor. Blood was analyzed for fatty acids, various hematological parameters, cytokines, PGE₂, and cortisol. Nasopharyngeal flush samples were analyzed for IgA. Composition of molasses blocks were found to be inconsistent with documented formulation and n-6:n-3 ratios were found to be similar between blocks. Therefore, main effect of hour was evaluated. White blood cells were elevated (P < 0.01) above baseline at subsequent sampling times and platelet count was elevated (P = 0.01) at h 1, 6, 24, 48, and 96 h post-stressor compared to baseline. Lymphocyte concentrations were reduced (P < 0.01) at h 1 compared to baseline but were elevated above baseline by h 6 (P < 0.01). Neutrophil concentrations were elevated (P < 0.01) at h 1 and 6 compared to baseline. Nasal mucosal IgA concentrations had a tendency (P < 0.07) to be greater at h 12 relative to baseline. Serum cortisol dropped to concentrations below baseline (P < 0.01) at h 1 and 6 but increased above baseline by h 12 (P < 0.01). It appears that stressors applied were sufficient to induce mild changes in several of the hematological parameters evaluated but an overall more profound effect may have been displayed if stressors were more profound or prolonged. A second experiment was conducted to evaluate fermentation characteristics and aerobic stability of whole-plant corn silage after inoculation with three different bacterial inoculants. Treatments consisted of Lactobacillus plantarum and Lactobacillus buchneri (LPB), L. plantarum, L. buchneri, and Lactobacillus diolivorans (LPBD), and L. diolivorans (LD). Silages were sampled on d 0, 14, 39, and 90. Propionate was elevated in silages inoculated with LPBD on d 39 and d 90 compared to other treatments (P < 0.03). 1, 2-propanediol was elevated (P < 0.01) in silages inoculated with LPB and LPBD on d 39 and d 90 compared to CON and LD silages. An effect of sampling day (P < 0.05) was detected for DM, aNDF, ADF, ash, starch, pH, total VFA, acetate, propionate, lactate, ethanol, and 1,2-propanediol. When silages sampled on d 14, 39, and 90 were subjected to an aerobic stability test, only treatment differences were detected (P < 0.05) for lactate: acetate and 1-propanol. Sampling day differences were detected (P < 0.05) for pH, total VFA, acetate, propionate, butyrate, lactate:acetate, 1-propanol, and 1, 2-propanediol. Overall, results suggest that epiphytic microflora populations largely dominated the ensiling process as indicated by the relative similarities in silages throughout the trial.

In this work, a model microbial agent for bioremediation was improved using fermentation and formulation methods. The outcomes of the fermentation work include the development of a new culture medium which increased the cell productivity greater than one order of magnitude. A robust functionality to degrade the herbicide atrazine was expressed. The new medium was scaled-up to a 2L bioreactor. Liquid bacterial culture was not inherently stable and lost viability at both 4°C and 25°C storage. When liquid bacterial culture was formulated by encapsulation in a biopolymer gel and applied to zeolite the transfer of cells from bacterial culture to formulated carrier was highly efficient. No loss of viability was measured from the immobilization process, and the functionality of the agent was retained. The formulated agent expressed an extended shelf life of at least 10 weeks when stored in ambient (25°C) temperature. When the formulation granules were inoculated into sterile soil, viability of the granules was stable and also retained the maximum level of functionality for the full test period of 10 weeks. The soil surrounding the formulation granules was also enumerated. The number of cells in the soil increased after a single inoculation of the formulation and the maximum level of functionality was conveyed from the formulation to the surrounding soil. The formulated inoculant constitutes an improvement for a bioremediation strain to stabilize the agent, produce an extended shelf life at ambient temperatures, and maintain the functionality of a microbe to utilize atrazine. In this thesis we have used a biopolymer formulation in which an inoculum is simply mixed into a gel and applied directly to the surface of the zeolite with no special equipment, drying, temperatures, or secondary re-growth steps required. It is a simple model system consisting of a carrier, and a artificial biofilm. As a technique to produce stable functional inoculants for bioremediation, the work presented here demonstrates an approach that is simple, practical, effective, and robust.

Soybean has a strong demand for nitrogen that can be acquired from atmosphere for vegetative growth and seed production through the symbiosis with the soil bacterium Bradyrhizobium japonicum (B. japonicum). However, the native soil bradyrhizobia may be ineffective in nitrogen fixation and the greatest limiting factor in increasing symbiotic nitrogen fixation is the inability to influence the infection of soybean roots by a desired strain of B. japonicum due to competition from the native bradyrhizobia. Previous studies have demonstrated the efficacy of a co-inoculum seed treatment on the symbiotic competency of the soybean cultivar LS90-1920 in greenhouse and field trials. The co-inoculation by the soil bacterium Streptomyces kanamyceticus (S. kanamyceticus) strain ATCC 12853 and strains of B. japonicum more efficient in nitrogen fixation and resistant to the antibiotics kanamycin and neomycin may have an advantage over the native bradyrhizobia regarding soybean root infection (Gregor et al., 2003). However, inconsistent inoculation responses in field trials and low efficacy in nodule competency by selected Bradyrhizobium japonicum (B. japonicum) co-inocula were observed under greenhouse conditions. These results were attributed to insufficient population size or growth of viable co-inocula associated with the seed treatments. This recent study showed that the nodulation response of LS90-1920 to B. japonicum strains KNI-1 and KNI-3 is independent of the inoculum dose and age of the broth culture. Iron supplement to the inoculum nutrient solution significantly increased the total biomass of nodules formed by strain KNI-1 but not by strain KNI-3 on a per plant basis and had no effect on the nodule number regardless of B. japonicum strain. In the glass bead viability study, the effect of inoculum nutrient solution concentration on the viability of bacterial co-inocula is species-specific and influenced by seed coating material. The growth of Pseudomonas putida strains displayed a dependency on the concentration of the inoculum nutrient solution with graphite or vermicompost as the seed coating material treatment or with activated charcoal treatment associated with 0.1% or 1.0 % inoculum nutrient solution. The seed coating material treatments of vermicompost and graphite promote stronger growth of S. kanamyceticus strain ATCC 12853 than the activated charcoal treatment. After a six-day incubation at 28oC, a 1.0 % inoculum nutrient solution maintained the highest viable populations of co-inocula with activated charcoal and a 0.1% inoculum nutrient solution was most effective in the maintenance of the co-inocula population when graphite or vermicompost was employed as the seed coating material. By applying the appropriate level of inoculum nutrient solution, the viability of a selected B. japonicum KNI strain and co-inocula remained stable for six days in activated charcoal and graphite treatment regardless of the number of applied co-inocula. However, the vermicompost treatment did not maintain the viable populations of the B. japonicum KNI strains and P. putida strain G11-32 but support the vigorous growth of S. kanamyceticus strain ATCC 12853 and P. putida strain 17-29. Greenhouse studies employing sterilized vermiculite as a soybean growth medium showed no significant differences in nodule competency by the inoculum/seed coating treatments associated with B. japonicum strain KNI-1. However, the co-inoculum treatments significantly increased either the total nitrogenase activity (B. japonicum strain KNI-3 with S. kanamyceticus strain ATCC 12853) or the nodule number (B. japonicum strain KNI-3 with S. kanamyceticus strain ATCC 12853 and P. putida strain 17-29) versus the singular inoculum treatment of strain KNI-3. Soil-pot studies under the same greenhouse conditions showed no significant differences in the nodule competency between the inoculum treatment of B. japonicum strain KNI-3, the co-inoculum treatment of strain KNI-3 and S. kanamyceticus, and the non-inoculated control regardless of seed coating material. However, co-inoculation of emergence-promoting rhizobacteria (Pseudomonas putida strain 17-29 and G11-32) with strain KNI-3 and S. kanamyceticus strain ATCC 12853 may improve the total nitrogenase activity and specific nitrogenase activity, depending on the seed coating material and soil type. The treatment with activated charcoal employed as a seed coating material and the co-inocula of strain KNI-3, S. kanamyceticus strain ATCC 12853 and P. putida strains 17-29 or G11-32 showed significantly higher total nitrogenase activity (Stoy silt loam) and specific nitrogenase activity (Drummer silty clay loam) versus the non-inoculated control. For the Bethalto silty clay loam, the same co-inoculum treatment associated with graphite and vermicompost as the seed coating material significantly increased the total nitrogenase activity. The seed coating treatment by activated charcoal enhanced nodulation competency for both the 2010 and 2011 field trials resulting in higher grain yield, seed nitrogen content, and seed protein content versus the seed coating treatment by graphite. No significant differences by the inoculum treatments were determined.

Utilizing local by-products can serve to significantly reduce the current feed costs for ruminant producers. The objective of this study is (1) to evaluate the effects of chemical and bacterial inoculants in combination with ensiling, on the nutritional value and ensiling compatibility of olive pomace (OP) and (2) to identify the most biologically favorable treatment and ensiling day of OP for feeding to cattle. Freshly centrifuged OP (Olivas de Oro Olive Company, Creston, CA) was treated with one of four treatments: (1) no additives (control; CONT); (2) urea applied at 5% of DM (UREA; Agrium U.S. Inc, Loveland, CO); (3) SiloSolve MC (SS; a mixture of Enterococcus faecium M74, Lactococcus lactis SR3.54, and Lactobacillus plantarum CH6072 applied at 1.5 x 105 cfu/g of fresh forage; Chr. Hansen A/S, Hørsholm, Denmark); (4) SiloSolve MC applied at the same rate as treatment 3 with calcium oxide (CAO; Flinn Scientific, Batavia, IL) applied at 5% of DM. Treated OP was ensiled in quintuplicates into 20-L laboratory silos with vents, which were sealed for 2, 10, 60, and 120 d in a completely randomized design. At 0 d, DM was 2 and 5% higher for UREA and CAO, respectively, while ash and pH were highest for CAO. Fat tended to be higher for SS and CP was highest for UREA by 60 d (23.8%). CAO had significantly lower fiber portions, ethanol and yeasts (P < 0.05), and higher acetic acid than CONT. No significant differences were observed at 12 and 24 h in situ compared to CONT, yet at 48 h, NDFD was greatest for CONT and SS (P < 0.001). Lactic acid increase with prolonged ensiling with UREA having the highest content at 120 d (0.716 %) and acetic decreased for CONT, SS, and CAO. SS contained the most LAB (8.5 log cfu/ fresh g). Yeasts, molds, and sugars significantly decreased with ensiling. In conclusion, OP was determined to be a viable nutritional source for ruminants given its chemical composition and effective preservation after 120 d of ensiling that could be improved with chemical and bacterial inoculants.

Three studies were conducted to assess if the ability of Enterococcus spp. surviving the ensiling process and bovine gastrointestinal tract could impact risk of bovine mastitis. The first study determined ability of enterococci to survive 3 wk ensiling. Grass and corn crops were divided into 3 treatments: 2 commercial silage inoculants, 1 negative control. After wk 1, 2, and 3 of ensiling, dry matter and bacterial enumeration were performed. Addition of silage inoculant led to greater levels of enterococci in grass silage compared with negative control levels, but showed less difference in inoculated corn silage. The second study quantified enterococci shedding rates in lactating dairy cows. Using a 4 x 4 Latin Square design, lactating, ruminally fistulated Holsteins were inoculated with enterococcal isolates from silage inoculants, ensiled forages, or clinical mastitis cases. Over the 4-period study, each period consisted of rumen and fecal sampling (2 wk) followed by a wash period (10 d). There were no significant differences in rumen or fecal enterococci levels between the 4 treatments. Both rumen and fecal enterococci levels showed significant differences between baseline and treatment periods (period 3, 4). The third study analyzed similarity in enterococcal isolates of silage and bovine origin using pulsed-field gel electrophoresis patterns from SmaI restrictions. Dendogram analysis showed none of the isolates met or were greater than a 75% genetic similarity and produced a genetically diverse population. Results suggest Enterococcus spp. from silage inoculants are not likely to contribute to an increased risk of enterococcal bovine mastitis. / Master of Science

O crescente aumento da população acarretará uma alteração na demanda por alimentos, tornando-se assim motivo de preocupação no âmbito da produção agrícola mundial, As mudanças climáticas constituem uma das mais sérias variáveis que podem prejudicar o processo produtivo de diferentes culturas de importância econômica, pois tais condições têm alterado o cenário ambiental global, tornando o estresse hídrico uma questão importante em todo o mundo. Esta condição tem sido apontada como um problema para o aumento produtivo de diferentes culturas. Assim, novas soluções para a sobrevivência e o crescimento das plantas sob a disponibilidade restrita de água são de importância central para maior eficiência agrícola. O uso de microrganismos simbióticos como inoculantes em culturas agrícolas pode contribuir com o sucesso desta atividade, pois diversos trabalhos têm relatado a presença de mecanismos adaptados a condições extremas podendo ser utilizados como ferramentas alternativas para aumentar a tolerância a seca das culturas que estarão associadas. Neste contexto, a busca por microrganismos com capacidade de sobrevivência em ambientes extremos, tem se apresentado como uma estratégia favorável na busca de opções para resolução destes problemas. Entretanto, pouco se sabe sobre bactérias simbióticas com a soja e os efeitos deste quando em condições de estresse hídrico. Desta forma, propõe-se com este estudo a descoberta de linhagens compatíveis que promovam crescimento de plantas de soja sob estresse hídrico e elucidação dos mecanismos que essas bactérias utilizam para induzir tolerância ao estresse. Dentro dessa abordagem, visa-se ainda o desenvolvimento de inoculante bacteriano em consórcio capaz de promover o crescimento e aumentar a produtividade de plantas da soja sob condições limitantes de água. / The increasing population will lead to a change in the demand for food, making it a major concern in the field of world agricultural production. Climate change is one of the most serious variables that can hinder the productive process of different crops of economic importance. such conditions have altered the global environmental scenario, making water stress an important issue throughout the world. This condition has been pointed out as a problem for the productive increase of different cultures. Thus, new solutions for the survival and growth of plants under the restricted availability of water are of central importance for greater agricultural efficiency. The use of symbiotic microorganisms as inoculants in agricultural crops may contribute to the success of this activity, since several studies have reported the presence of mechanisms adapted to extreme conditions and can be used as alternative tools to increase the drought tolerance of the crops that will be associated. In this context, the search for microorganisms with the ability to survive in extreme environments has been presented as a favorable strategy in the search for options to solve these problems. However, little is known about symbiotic bacteria with soybean and its effects when under stress conditions. Thus, it is proposed with this study the discovery of compatible strains that promote the growth of soybean plants under water stress and elucidation of the mechanisms that these bacteria use to induce tolerance to stress. Within this approach, the development of a bacterial inoculant in a consortium capable of promoting growth and increasing the productivity of soybean plants under water limiting conditions.

O Estado do Tocantins apresenta área de cerrado, possuindo um grande potencial para o desenvolvimento do setor agropecuário. Ele concentra suas principais atividades na pecuária e na produção de grãos. Os principais fatores de baixa produtividade das culturas agrícolas, tais como, a soja, são as doenças causadas por patógenos que habitam o solo, que podem provocar prejuízos severos com perdas significativas na produtividade. O tratamento biológico em sementes com microrganismos benéficos é muito pouco conhecido. O fungo Pochonia vem se destacando por apresentar um potencial como agente de controle biológico e ser bastante promissor como promotor de crescimento em plantas. A inoculação de sementes com o fungo Pochonia, não só protege as plantas contra várias doenças, mas também, poderá melhorar o crescimento, podendo agir como promotor do crescimento vegetal. Objetivou-se neste presente trabalho determinar a capacidade de Pochonia spp. em sintetizar ácido indol acético (AIA), capacidade de solubilização de fosfato e a promoção do crescimento vegetal em culturas como soja e feijão-caupi. Foram determinadas a capacidade de sintetizar AIA e capacidade de solubilização de fosfato, meio BD adicionado de solução de K2HPO4 e CaCl2. Os resultados obtidos nas avaliações de AIA e solubilização de fosfato mesmo que em pequenas quantidades apresentaram incrementos em relação a testemunha, apresentaram resultados que variaram de 0,77 a 7,45 μg mL-1 em AIA e de 3,48 a 13,42 μg mL-1 na solubilização de fósforo, sendo que as estirpes que obtiveram melhor resultado nos experimentos foram UFT- P02 e UFT-P04. Nas avaliações em casa de vegetação avaliou-se MPSA, MSR, MST e altura de planta aos 45 e 56 dias após a emergência, dentre as estirpes as que se mostram com melhor eficiência relativa em relação a testemunha foram as estirpes UFT-P05 e UFT-P03 com incremento de 77 e 75% respectivamente. No teste de doses desenvolvido a campo obteve-se incremento 1.083,7 kg ha-1 em relação a testemunha sendo a dose que obteve maior incremento foi na dose de 3 kg de inoculante / 50 kg de semente com produtividade total de 4.115,7 kg ha-1. Conclui se com estes estudos que a Pochonia spp. mostrou potencial em disponibilizar fosforo para planta, sintetizar AIA com isso promovendo maior crescimento vegetal, incrementando assim aumentos significativos na produtividade de grãos. / The State of Tocantins presents a cerrado area, possessing great potential for the development of the agricultural sector. It focuses its main activities on livestock and grain production. The main factors of low productivity of agricultural crops, such as soybeans, are diseases caused by pathogens that inhabit the soil, which can cause severe losses with significant losses in productivity. Biological treatment in seeds with beneficial microorganisms is very little known. The Pochonia fungus has been shown to have a potential as a biological control agent and is very promising as a growth promoter in plants. Seed inoculation with the Pochonia fungus not only protects plants against various diseases, but also can improve growth and can act as a promoter of plant growth. The objective of this work was to determine the capacity of Pochonia spp. to synthesize indole acetic acid (IAA), phosphate solubilization capacity and promote plant growth in crops such as soybean and cowpea. The ability to synthesize IAA and phosphate solubilization capacity, BD medium added with K2HPO4 solution and CaCl2 were determined. The results obtained in the evaluations of IAA and phosphate solubilization even though in small quantities presented increases in relation to the control, presented results ranging from 0.77 to 7.45 μg mL-1 in AIA and from 3.48 to 13.42 μg mL-1 in phosphorus solubilization, and the strains that obtained the best result in the experiments were UFT-P02 and UFT-P04. DMA, DMR, DMT and plant height were evaluated in the greenhouse evaluations at 45 and 56 days after emergence, among the strains which showed the best relative efficiency in relation to the control were strains UFT-P05 and UFT -P03 with an increase of 77 and 75% respectively. In the dose test developed in the field, an increase of 1,083.7 kg ha-1 was obtained in relation to the control, and the dose that obtained the greatest increment was the dose of 3 kg of inoculant / 50 kg of seed with total yield of 4,115.7 kg ha-1. It is concluded from these studies that Pochonia spp. showed a potential in providing phosphorus to the plant, synthesizing IAA with this, promoting greater plant growth, thus increasing significant increases in grain yield.

O crescente aumento da população acarretará uma alteração na demanda por alimentos, tornando-se assim motivo de preocupação no âmbito da produção agrícola mundial, As mudanças climáticas constituem uma das mais sérias variáveis que podem prejudicar o processo produtivo de diferentes culturas de importância econômica, pois tais condições têm alterado o cenário ambiental global, tornando o estresse hídrico uma questão importante em todo o mundo. Esta condição tem sido apontada como um problema para o aumento produtivo de diferentes culturas. Assim, novas soluções para a sobrevivência e o crescimento das plantas sob a disponibilidade restrita de água são de importância central para maior eficiência agrícola. O uso de microrganismos simbióticos como inoculantes em culturas agrícolas pode contribuir com o sucesso desta atividade, pois diversos trabalhos têm relatado a presença de mecanismos adaptados a condições extremas podendo ser utilizados como ferramentas alternativas para aumentar a tolerância a seca das culturas que estarão associadas. Neste contexto, a busca por microrganismos com capacidade de sobrevivência em ambientes extremos, tem se apresentado como uma estratégia favorável na busca de opções para resolução destes problemas. Entretanto, pouco se sabe sobre bactérias simbióticas com a soja e os efeitos deste quando em condições de estresse hídrico. Desta forma, propõe-se com este estudo a descoberta de linhagens compatíveis que promovam crescimento de plantas de soja sob estresse hídrico e elucidação dos mecanismos que essas bactérias utilizam para induzir tolerância ao estresse. Dentro dessa abordagem, visa-se ainda o desenvolvimento de inoculante bacteriano em consórcio capaz de promover o crescimento e aumentar a produtividade de plantas da soja sob condições limitantes de água. / The increasing population will lead to a change in the demand for food, making it a major concern in the field of world agricultural production. Climate change is one of the most serious variables that can hinder the productive process of different crops of economic importance. such conditions have altered the global environmental scenario, making water stress an important issue throughout the world. This condition has been pointed out as a problem for the productive increase of different cultures. Thus, new solutions for the survival and growth of plants under the restricted availability of water are of central importance for greater agricultural efficiency. The use of symbiotic microorganisms as inoculants in agricultural crops may contribute to the success of this activity, since several studies have reported the presence of mechanisms adapted to extreme conditions and can be used as alternative tools to increase the drought tolerance of the crops that will be associated. In this context, the search for microorganisms with the ability to survive in extreme environments has been presented as a favorable strategy in the search for options to solve these problems. However, little is known about symbiotic bacteria with soybean and its effects when under stress conditions. Thus, it is proposed with this study the discovery of compatible strains that promote the growth of soybean plants under water stress and elucidation of the mechanisms that these bacteria use to induce tolerance to stress. Within this approach, the development of a bacterial inoculant in a consortium capable of promoting growth and increasing the productivity of soybean plants under water limiting conditions.

Due to population expiation and the increased awareness of the impact on the environment by wastewater treatment, improved wastewater treatment systems are needed to treat municipal and agricultural wastewater. Treating wastewater with oxygen decreases carbon compounds at the expense of energy to move carbon and oxygen to be in contact with each other. Anaerobic digestion of wastewater can reduce the cost by utilizing microbes to treat high amounts of carbon in wastewater without the need for extensive oxygen requirement. With a proper balance of nutrients, microbes also produce methane, a renewable energy source. It has been suggested that microalgae be utilized to help balance the nitrogen content of wastewater for treatment by microbes. One challenge with the use of algae is the initial breakdown of algae cells. Using a digester with microorganism that can produce methane and decompose algae could enhance production of methane from the digestion of algae. The combination of wastewater, which is high in carbon content relative to nitrogen, with algae, which is high in nitrogen, could provide the balance needed for the microbes to treat wastewater and provide methane. A biomethane potential test was used to compare the ability of two microbe communities, facultative lagoon sediment and anaerobic digester sludge to digest algae. Each microbe community treated dairy, swine, municipal, and petrochemical wastewater augmented with algae and acetate. The ability to degrade augmented wastewater and produce methane was determined by measuring the volume and composition of biogas produced over time. Both treatments were successful in production of methane. Facultative lagoon sediment showed more methane produced per carbon dioxide than anaerobic digester sludge.

Made available in DSpace on 2014-06-11T19:32:54Z (GMT). No. of bitstreams: 0 Previous issue date: 2009-02-13Bitstream added on 2014-06-13T18:44:28Z : No. of bitstreams: 1 scaquitto_dc_dr_jabo.pdf: 429936 bytes, checksum: 3e636e555a2a2cd56aa2c19a9d5c4352 (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / O amendoim (Arachis hypogaea L.) faz parte da família Fabaceae, que é capaz de associar-se com bactérias do gênero Rhizobium. Em solos onde o amendoim é cultivado tem-se a presença de uma população autóctone desta bactéria. A necessidade de inoculação das sementes de amendoim em solos contendo população autóctone tem sido sempre discutida. Com o objetivo de avaliar o efeito da inoculação de sementes de amendoim cultivado em vasos contendo vermiculita e a interação existente entre microrganismo-planta e microrganismo-microrganismo, foi realizado um experimento sob cultivo em casa de vegetação, em vasos de 0,5 L, com o cultivar IAC 886 e a IAC Tatu-ST, com três repetições. Os tratamentos foram constituídos de aplicação dos isolados individualmente e combinados com a estirpe padrão tipo SEMIA 6144, Bradyrhizoibium sp., recomendada pelo Ministério da Agricultura e sem aplicação de inoculante, sem aplicação de qualquer isolado. Houve interação entre o tipo de plantio e a aplicação de inoculante para todas as variáveis analisadas. Para a massa de matéria seca da parte aérea houve uma diferença significativa. O variável número de nódulos e o teor de Nitrogênio também apresentam diferenças quanto à aplicação, sendo que, em todos os casos foram superiores ao controle sem aplicação com os isolados nativos. / Peanut (Arachis hypogaea L.) belonging to Fabacea family, is able of associating with bacteria from Rhizobium genus. In soils where peanut is grown, there is usually an autochthonous population of these bacterial, however, the need of inoculation in peanut seeds in soils containing autochthonous population has always been controversial subject. With the objective of evaluating the effects of peanut seeds inoculation cultivated in vases contend vermiculite and to verifying the interaction with plant regulators, an experiment was carried out in greenhouse conditions, with three repetitions containing Runner IAC 886 e IAC Tatu-ST cultivar. The treatments were constituted of application of the individually isolated and combined with the lineage standard Bradyrhizobium sp., SEMIA 6144, recommended by Bureau of the Agriculture. An interaction was observed between the type of plantation and the application of inoculants for all analysed variable. For the mass of dry matter of the aerial part it had a significant difference. The variable numbers of nodules and rate of Nitrogen also present differences related to the treatments, being that, in all the cases was greater to the control without application of the isolated natives.