Thesis (M. Phil.)--University of Hong Kong, 2001. / Includes bibliographical references (leaves 149-174).

The objective of this study was to develop a reliable method for intracytoplasmic sperm injection (ICSI) in bovine oocytes. Oocytes recovered from abattoir-derived ovaries were centrifuged for 5 min at 6000xg to facilitate sperm injection. Sperm were pre-treated in vitro with 5mM dithiothreitol (DTT), and diluted (approximately 1:5) with 5% polyvinylpyrrolidone (PVP) in 0.9% saline. After sperm injection, various activation procedures were compared. Initially, 3 h after activation with 5muM Ionomycin (A23187), oocytes with second polar bodies were selected and treated with 1.9mM 6-dimethylaminopurine (DMAP). The cleavage rate of sperm-injected oocytes treated with Ionomycin and DMAP was higher than with Ionomycin alone (62.1 vs. 27.3%, p < 0.05). In sham-injected control oocytes treated with Ionomycin and DMAP, the cleavage rate was approximately six times higher than that of oocytes treated with Ionomycin alone (44.3 vs. 7.4%, p < 0.001). Upon examination 16 h after ICSI, pronuclear formation was observed in 33 of 47 (70.2%) DMAP-treated oocytes. Two pronuclei were present in 18 of 33 (54.6%), while one and three pronuclei were seen in 8 of 33 (24.2%) and 7 of 33 (21.2%), respectively. In sham-injected DMAP-treated control oocytes, 6 of 15 (40.0%) had one pronucleus while 9 of 15 (60.0%) had two pronuclei. Since a single Ca2+ stimulation provided insufficient activation and DMAP treatment could result in triploidy, activation by multiple Ca2+ stimulations following ICSI was tested. Three Ca2+ stimulations were given at 30-min intervals. Pronuclear formation was observed in 16 of 41 (39.0%) oocytes at 16 h after sperm injection, with one and two pronuclei found in 4 of 16 (25.0%) and 12 of 16 (75.0%), respectively. Although one pronucleus was formed in 3 of 33 (9.1%) sham-injected control oocytes treated with three Ca2+ stimulations, two pronuclei were not seen in any of these oocytes. Due to the low rate of pronuclear formation after 5muM Ionomycin, 50muM

No description available.

This thesis explores the complex web of social relations created by the use of donor insemination (DI) in Aotearoa/New Zealand. The experiences of pursuing parenthood and creating a family using this method of assisted conception are contextualised through attention to the practices of Donor Insemination Programmes and the discourses used by parents, their families and health professionals. Sociologists and other social scientists have drawn attention to the social and cultural consequences of the fragmentation of biological/genetic, gestational and social parenting that follows the use of third party gametes. This thesis explores the implications of these procreative arrangements for the meanings attached to cultural concepts such as 'kinship', 'family', and 'parenthood'. Variation in the way these families respond to issues associated with the use of donor sperm in the conception of a child is also highlighted. The thesis also explores the dominant discourse in the New Zealand context of children's 'right' to know their genetic origins, and how this is played out in the perceptions and actions of health professionals, parents of children conceived by DI and their kin. The research is exploratory and qualitative, drawing on semi-structured interviews with parents, grandparents, aunts and uncles of children conceived by DI, and with health professionals working in DI programmes. The inclusion of the perspectives of extended family members and health professionals constitutes a unique contribution to research on families with children conceived by DI. The secrecy, anonymity and confidentiality that have surrounded DI practices have long hindered the study of families with children conceived by DI. Despite a trend towards information-sharing in DI in New Zealand, the thesis shows that for these families, patterns of secrecy and disclosure are complex, variable and embedded in particular social and relational contexts.

No description available.

No description available.

Thesis (M.S.)--University of Wisconsin--Madison, 1965. / eContent provider-neutral record in process. Description based on print version record. Bibliography: l. 26-29.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1956. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Bibliographies: leaves 36-40, 92-103.

Thesis (M.S.W.)--Smith College School for Social Work, Northampton, Mass., 2009. / Includes bibliographical references (p. 88-90).

Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1992. / Vita. Abstract. Includes bibliographical references (leaves 54-59). Also available via the Internet.