Submitted by Milena Rubi (milenarubi@ufscar.br) on 2017-08-08T17:44:33Z No. of bitstreams: 1 GHIOTTO_Thais_2016.pdf: 18907197 bytes, checksum: 0779f8ac869df152bdd7747df97a15e5 (MD5) / Approved for entry into archive by Milena Rubi (milenarubi@ufscar.br) on 2017-08-08T17:44:43Z (GMT) No. of bitstreams: 1 GHIOTTO_Thais_2016.pdf: 18907197 bytes, checksum: 0779f8ac869df152bdd7747df97a15e5 (MD5) / Approved for entry into archive by Milena Rubi (milenarubi@ufscar.br) on 2017-08-08T17:44:49Z (GMT) No. of bitstreams: 1 GHIOTTO_Thais_2016.pdf: 18907197 bytes, checksum: 0779f8ac869df152bdd7747df97a15e5 (MD5) / Made available in DSpace on 2017-08-08T17:44:55Z (GMT). No. of bitstreams: 1 GHIOTTO_Thais_2016.pdf: 18907197 bytes, checksum: 0779f8ac869df152bdd7747df97a15e5 (MD5) Previous issue date: 2016-05-09 / Não recebi financiamento / Megastigmus transvaalensis is an exotic wasp that attacks Brazilian pepper tree drupes Schinus terebinthifolius in native forests and areas of restoration and ecological restoration in Brazil. The parasitism begins with the oviposition M. transvaalensis in drupes S. terebinthifolius where the larvae hatch and remain internally feeding of nutrients and tissue, affecting germination. The aim of this work was to study the effect of temperature, rainfall and humidity in the population dynamics of M. transvaalensis and determine the parasitism rate and sex ratio of this wasp in drupes S. terebinthifolius. The study was performed with yellow sticky traps and collection of S. terebinthifolius drupes in seasonal semideciduous forest during August 2014 to September 2015, in the region of Sorocaba, São Paulo, Brazil. The capture of insects through the sticky traps proved satisfactory, and thus can be applied to the monitoring of small Hymenopteros. Populations of M. transvaalensis were negatively correlated with the maximum temperature and population peak in the fall of 2015, meaning that when the maximum temperature has reduced, there is a higher incidence of insect field. The parasitism rate of S. terebinthifolius drupes ranged from zero to 36.34% during this period, making this value an aggravating factor for the emergence of new individuals, since this phytophagous wasp has the potential to be spread throughout Brazil and It poses a threat to the natural regeneration of S. terebinthifolius. The sex ratio of M. transvaalensis was 0.42 and 0.08 in the laboratory field. The bio-ecology and the damage caused by M. transvaalensis in drupes S. terebinthifolius warrant further studies to integrated management / Megastigmus transvaalensis é uma vespa exótica que ataca drupas de aroeira-pimenteira Schinus terebinthifolius em florestas nativas e em áreas de recomposição e restauração ecológica no Brasil. O parasitismo inicia com a oviposição de M. transvaalensis nas drupas de S. terebinthifolius, onde as larvas internamente eclodem e permanecem alimentando-se dos nutrientes e tecidos, prejudicando a germinação. O objetivo desse trabalho foi estudar o efeito da temperatura, precipitação e umidade na flutuação populacional de M. transvaalensis e determinar o índice de parasitismo e razão sexual dessa vespa nas drupas de S. terebinthifolius. O estudo foi realizado com armadilhas adesivas amarelas e coleta de drupas de S. terebinthifolius em fragmento de floresta estacional semidecidual, durante agosto de 2014 a setembro de 2015, na região de Sorocaba, São Paulo, Brasil. A captura dos insetos através das armadilhas adesivas se mostrou satisfatória, podendo assim ser aplicada para o monitoramento de pequenos Hymenopteros. As populações de M. transvaalensis apresentaram correlação negativa com a temperatura máxima e pico populacional no outono de 2015, significando que, quando a temperatura máxima apresenta redução, há maior incidência do inseto em campo. O índice de parasitismo das drupas de S. terebinthifolius variou de zero a 36,34% no período avaliado, tornando este valor um agravante para o surgimento de novos indivíduos, uma vez que, essa vespa fitófaga tem potencial de ser disseminado por todo Brasil e representa ameaça para a regeneração natural de S. terebinthifolius. A razão sexual de M. transvaalensis foi de 0,42 no laboratório e 0,08 em campo. A bioecologia e os danos causados por M. transvaalensis em drupas de S. terebinthifolius justificam mais estudos visando o manejo integrado dessa vespa fitófaga.

[pt] Atualmente, as novas tecnologias estão estendendo os limites físicos dos veículos automotivos em busca de mais segurança e comforto. Novas aplicações, como por exemplo veículos autônomos, exigem sistemas de controle capazes de garantir a estabilidade do veículo durante a condução autônoma ou em cenários perigosos. Na maioria dos carros modernos, os sistemas de controle atuam de forma independente, ou seja, não há coordenação ou compartilhamento de dados entre eles, pois poderiam produzir conflitos entre esses controladores. Desse modo, nenhuma melhoria na estabilidade do veículo é alcançada ou inclusive, piores cenários podem ser produzidos. Para superar esses problemas, uma abordagem integrada é projetada neste trabalho. Esta integração, definida como sistema de controle integrado (IC), usa uma regra para coordenar o programa eletrônico de estabilidade (ESP em inglês) e o sistema de direção de quatro rodas (4WS em inglês). O ESP realiza uma frenagem seletiva dependendo do estado atual do veículo. Esta condição é estimada pela diferença entre a taxa de guinada desejada, obtida usando um modelo linear do veículo, e a taxa de guinada real. Adicionalmente, as pressões de frenagem em cada roda são calculadas pelo sistema de travagem antibloqueio (ABS em inglês). Neste trabalho, uma lógica de comutação on-off e um modelo hidráulico de primeira ordem são empregadas para modelar o sistema ABS. Para projetar o 4WS, usou-se uma estratégia por alimentação direta que considera o ângulo de esterçamento das roas frontais. Finalmente, para testar as vantagens do sistema IC proposto nesta tese contra o enfoque não integrado, realizaram-se simulações considerando um modelo não-linear do veículo em cenários críticos de condução. O modelo do veículo foi derivado empregando a abordagem multicorpos e o princípio de Jourdain, e depois é validado usando um conjunto de dados experimentais obtidos por sensores montados em um carro a escala. / [en] Nowadays new technologies are pushing the road vehicle limits further. Promising applications, e.g. self-driving cars, requires control systems that are able to ensure the vehicle s stability during autonomous driving or under dangerous scenarios. In most of modern cars, the control systems actuates independently, i.e. there is no coordination or data sharing between them. This approach can produce conflicts between these standalone controllers, thereby no improvements on the vehicle s stability are achieved or even a worse scenario can be produced. In order to overcome these problems, an integrated approach is designed in this work. This integration, defined as Integrated control system (IC), use a rule to coordinate the Electronic stability program (ESP) and the Four-wheel steering system (4WS). The ESP performs a selective braking depending of the current state of the vehicle. This condition is estimated by the difference between the desired yaw rate, obtained using a linear vehicle model, and the actual yaw rate. In addition, the braking pressures at each wheel are computed by the Anti-lock braking system (ABS). In this work, an on-off switching logic and a firstorder hydraulic model are employed to model the ABS system. To model the 4WS, a simple feed-forward control strategy that consider the front steering as input is used. Finally, in order to test the advantages of the IC system against the non-integrated one, simulations considering a nonlinear vehicle model under critical driving scenarios were performed. The vehicle model was derived employing the multibody approach and the Jourdain s principle, and then it is validated using a set of experimental data obtained by sensors mounted on a scaled car.

There is a growing recognition globally that many agrochemicals are hazardous to humans, animals and the environment. Therefore, there is a need to substitute these chemical products with biological and physical treatments, and to change agronomic practices in order to control pests and diseases in agriculture. The primary objective of this thesis was to develop and test in laboratory, field and commercial packhouses trials as alternative control measures against green mould of citrus (caused by Penicillium digitatum Pers: Fr. Sacc) and Penicillium molds of litchi (caused by several Penicillium). A South African isolate of P. digitatum, isolated from an infected orange fruit, was found to be resistant to imazalil (the standard postharvest fungicide used in South Africa). Sixty yeast and 92 Bacillus strains were screened for their antagonistic activity against this isolate of P. digitatum. None of the yeasts or Bacillus isolates produced a curative action against P. digitatum on oranges. However, yeast Isolate B13 provided excellent preventative control of P. digitatum, superior to all the Bacillus isolates, when it was applied to citrus fruit prior to artificial inoculation with P. digitatum. Electron microscopy showed that yeast Isolate B13 inhibited conidial germination of P. digitatum. For the control of P. digitatum pre-harvest, trees were sprayed with a yeast, Isolate B13, a few months or a few days before harvest. However, this treatment alone proved to be ineffective in providing preventative control of green mould on Valencia oranges. For the control of P. digitatum preharvest, trees were treated with potassium silicate for a full season. Regular potassium silicate treatments resulted in a significant preventative control of P. digitatum infection on both navel and Valencia oranges. Treatment of Eureka lemons with potassium silicate as a postharvest treatment for the control of P. digitatum resulted in reduced disease lesion diameters when applied preventatively or curatively. Electron microscopy showed that potassium silicate inhibited germination of P. digitatum conidia and growth of its mycelium. Hot-water dip treatment at 50-58°C for 60-180 seconds (in increments of 15 seconds), significantly reduced infection development in inoculated wounds of Valencia oranges compared with control fruit treated with tap water, without causing any rind damage. The integration of the yeast, a hot water dip and potassium silicate pre-and postharvest applications provided control of P. digitatum control in multiple packhouse trials. The control achieved by the yeast Isolate B13 or hot-water, and potassium silicate in the packhouse alone was superior or equivalent to that provided by imazalil. A similar study was also carried out to determine possible control measures for Penicillium sp. on litchis. In this study, a total of 23 yeast and 13 Bacillus isolates were obtained from litchi fruit surfaces. Ten yeast and 10 Bacillus isolates that had shown good efficacy against P. digitatum of citrus were added to these for screening against Penicillium sp. of litchis. None of the yeasts or Bacillus isolates produced a curative action against Penicillium sp. infection on litchis. However, several yeast isolates (YL4, YL10, YLH and B13) resulted in reduced severity of the pathogen, when applied preventatively, compared with an untreated control. The yeast isolates were superior to all the Bacillus isolates, when applied to litchis prior to artificial inoculation by Penicillium infection on litchis. Potassium silicate as a postharvest treatment for the control of the pathogen caused reduced lesion diameters when applied preventatively or curatively to naturally infected litchis. The results presented in this thesis highlight the use of biological, physical and agronomic practices singly or in combination as an alternative control strategy against citrus postharvest green mould. This thesis also provides an insight into expanding these strategies, partly or fully, for the control of other postharvest Penicillium infections using litchi as an example. / Thesis (Ph.D.)-University of KwaZulu-Natal, Pietermaritzburg, 2010.

Weed management in sustainable farming systems often requires the integration of several different pest management techniques. Cultural, biological, and chemical pest control efforts can be utilized to contribute to the common goal of reducing weeds in vegetable row crop systems. This research addresses how common disturbances such as tillage and insecticide use affect: carabid beetle assemblages; predation of weed seeds by invertebrates; and weed seed recruitment. Field experiments were conducted over three years at two different locations on the OSU Vegetable Research Farm, Linn Co., OR. We found that activity-density of carabid beetles varied seasonally and peaked in late August-September each year. Insecticide applied in year 1 affected seed loss in year 2, suggesting possible long-term effects of land management on weed seed removal. Weed recruitment was highly variable between treatment, site, and year. Conserving biological weed control agents in combination with cultural techniques such as reducing tillage and the use of cover crops, helps growers shift from expensive, density-independent control efforts to more ecological, long-term solutions for weed management in agroecosystems. / Graduation date: 2011 / Access restricted to the OSU community, at author's request, from Dec. 15, 2010 - Dec. 15, 2011.