Invasive species are one of many important environmental issues facing Canadians today. A great deal of research has explored both the scientific and social aspects of invasive species. However, the cumulative research has not yet thoroughly explored people’s thoughts and feelings about, or perceptions of, invasive species and the influence these may have on management of, or policy decisions regarding, invasive species. This thesis research project was designed to assess the attitudes and perceptions that high school students have towards invasive species and to determine to what extent learning about invasive species alters their connection with nature. The study group was comprised of students from four Grade 11 Environmental Science classes from three high schools within the city of Guelph, Ontario. The students received regular classroom instruction from their teachers covering the course content, including invasive species. Students also made weekly visits to a local nature centre, providing them with hands-on learning experiences related to the course content. Students responded to an 80-question survey that assessed their knowledge of local invasive species and attitudes towards them as well as students’ connection to nature. Their connection to nature was assessed using a modified version of the Connectedness to Nature Scale (Mayer & Frantz, 2004). The surveys were administered by the classroom teachers in October, 2010, prior to the presentation of instructional material covering invasive species and then again in December, 2010 when the presentation of instructional material concerning invasive species was complete. The results showed that the students’ knowledge of invasive species upon entering the course was quite low and each of the classes witnessed a significant increase in knowledge. The survey results did not reveal any change to the students’ connection to nature; however, they did reveal three underlying themes in students’ attitudes towards invasive species: a concern about invasive species as a threat or problem; feelings of acceptance towards invasive species; and feelings of anxiety about invasive species. These attitudes remained relatively unchanged after the course.

Roadsides in Sweden offer suitable habitats for species that naturally occur in the old agricultural landscape, a habitat that is less common today. These species are favored by small-scale disturbance such as of mowing which is to some extent applied in roadsides for management of the road surface. The Swedish Transport Administration has worked according to the environmental directives for sustainable populations of threatened species that occur along roadsides. Roadsides with threatened species and species that represent the old agriculture landscape have been located and described. Such roadsides have been named species rich roadsides (artrik vägkant) and can be found in different environments spread across the country. The Swedish Transport Administration has developed recommendations for special management to support species of old agricultural landscape in roadsides. Previous studies have shown a negative development of the species rich roadsides, with reduced numbers of species rich roadsides.  This project has analyzed whether there is a relationship between the management of species rich roadsides and the negative development of species rich roadsides, and also make recommendations for improving management methods needed for improving the development of species rich roadsides. Data have been used from previous inventories of species rich roadsides, the study area restricted to Region Mitt.  The present management of species rich roadsides has a significant negative effect of the development of species rich roadsides. To improve the development of species rich roadsides, management must be adapted to the environment the roadside is located to.

The horticultural industry is responsible for approximately half of the invasive plant introductions in North America. To reduce these introductions, voluntary initiatives are preferred over government regulations. This thesis aims to evaluate the effectiveness of two types of voluntary initiatives. At the gardener level, I investigated the effectiveness of alternative species promotion campaigns, called “Grow Me Instead” programs. Adult gardeners visiting the Royal Botanical Gardens in Hamilton, Ontario, as well as customers at two garden centres, participated in a conjoint analysis which measured their preferences for various traits of potential ground cover species. Results showed that gardeners generally prefer plant species having invasive characteristics, suggesting these programs may not be as effective as initially believed. At the retailer level, this study aimed to build upon the work done by Burt and colleagues (2007) to obtain further understanding of the relative strength of internal (ethical motivations) and external (legislation, stakeholder pressures and economic opportunities) factors for motivating participation in voluntary initiatives. Telephone interviews were conducted with 30 industry professionals from southern Ontario to assess their adoption of the St. Louis Voluntary Codes of Conduct. Results found that participation rates of industry professionals in southern Ontario were lower for every specific initiative than those interviewed by Burt et al. (2007). Industry professionals presently experience the most pressure to participate from a sense of personal responsibility and the desire to create a green business image. Pressure was significantly higher from these sources than from pressure from employees. Together these two studies identified several barriers to the efficacy of voluntary initiatives as well as some reasons for optimism. To ensure the success of future voluntary initiatives, efforts must be made to encourage these two groups to work together. Understanding how both retailers and gardeners respond to voluntary initiatives will assist in the development of more effective programs and lead to fewer horticultural invasive species introductions in the future.

Feral swine (Sus scrofa domesticus) are spreading across North America at an alarming rate. Four Canadian provinces and 39 states within the continental United States now report standing populations of feral pigs. Estimates place the number of feral hogs in Texas >2M, accounting for more than half of the United States population. It is known that feral swine impact local ecology following establishment, with regard to shifts in local flora and fauna. The overall objective of this research was to investigate the role of feral swine in tick-borne pathogen transmission in Texas. The underpinning objectives were to establish host records for tick species parasitizing feral swine, determine the species assemblages associated with feral swine among different ecoregions of Texas, determine by immunoassay to which tick-borne bacteria feral pigs were being exposed, and detect the DNA of tick-borne bacteria by polymerase chain reaction assay in the event of poor or early immune response by the host. Feral pigs (N=432) were harvested from June 2008 to June 2010 using box and corral traps and by aerial gunning. Seven species of ticks, Amblyomma americanum, A. cajennense, A. maculatum, Dermacentor albipictus, D. halli, and D. variabilis; and Ixodes scapularis, were collected. Immature stages of A. cajennense and A. americanum were collected as well. All classes of feral pigs, gender by age, were infested with ticks. Serum was collected through a multi-organizational effort from 2006 to 2010 and tested by ELISA for previous exposure to tick-borne pathogens in the genera Rickettsia and Ehrlichia (N=888) and Borrelia (N=849). Prevalence percentages by immunoassay were 27.59 percent, 13.18 percent and 2.12 percent for Rickettsia, Ehrlichia, and Borrelia, respectively. Samples positive by ELISA for exposure to Borrelia were further screened by Western Blot for exposure to Borrelia turicatae. The results were equivocal in most cases. Blood samples (N=233) were collected from 2008 to 2010 and analyzed by polymerase chain reaction for the detection of the DNA of these same three genera of bacteria. Two of the samples were positive by PCR for the presence of Borrelia DNA. Texas feral swine are serving as hosts for at least seven species of ticks and are interacting with tick-borne pathogen transmissions cycles in Texas.

Introduced species cause both considerable ecological and economic damage every year. However, not much is known about how certain species are able to establish and spread beyond the site of initial introduction, whereas others do not. Species undergoing range expansion following an introduction may prove to be a valuable resource to invasion biology, but may also be informative in light of species' responses to changing environments (i.e. global climate change). Here, I took advantage of an ongoing range expansion of an introduced vertebrate species. House sparrows (Passer domesticus) were introduced to Mombasa, Kenya in the 1950s and have subsequently expanded their range northwest-ward and now occupy most major cities in Kenya. By comparing older, established populations (i.e. those in Mombasa) with more recently colonized populations at the range edge, it might be possible to determine some of the mechanisms that underlie range expansion in some species and/or populations. In Chapter 1, the background and ideas that motivated the rest of the dissertation is summarized. In Chapter 2, I studied how exploration and glucocorticoids (a hormone released in response to stressors) changed throughout the range expansion. Exploration was greater at the range edge, which is likely to ensure greater discovery of novel resources. Glucocorticoids released in response to restraint were also highest at the range edge, which might facilitate resolution of stressors in unpredictable environments. However, chronically elevated levels of glucocorticoids are often considered maladaptive, unless an individual can appropriately cope with them. Therefore, in Chapter 3, I characterized glucocorticoid receptors (i.e. mineralocorticoid receptor (MR) and glucocorticoid receptor (GR)) in the hippocampus, an area responsible for negative feedback of glucocorticoids as well as induction of behavioral and physiological response to stressors. I found that MR density was lower relative to GR density at the range edge compared to the site of introduction (Mombasa). I speculate this pattern is a mechanism to resolve the elevated levels of glucocorticoids at the range edge. Taken together, these results indicate that individuals at the range edge have a strong glucocorticoid response to stressors to induce a rapid, strong response to resolve stressors. Subsequently, in Chapter 4, I examined the potential mechanisms of phenotypic change among Kenyan house sparrows. Typically, following an introduction event, genetic diversity undergoes a bottleneck and is greatly reduced compared to the source population; as such, genetic evolution as the main driver of changing phenotypes throughout the range expansion is unlikely. We therefore hypothesized that epigenetic mechanisms (e.g. DNA methylation) may compensate for the expected reduced genetic diversity following an introduction. Although there was no pattern of epigenetic variation among cities (i.e. variation did not increase nor decrease further from the site of introduction), epigenetic variation increased as genetic inbreeding increased (a sign of reduced genetic diversity and bottlenecks), suggesting epigenetic modifications may compensate for reduced genetic diversity following an introduction event. Overall, patterns of phenotypic variation emerged dependent on age of the population- these patterns may prove to be important in other vertebrate range expansions as well. Surprisingly, epigenetic diversity did not correlate with phenotypic variation among populations; however, within-individual studies may reveal epigenotypes are related to certain behavioral or physiological phenotypes. In the future, studies should be designed to address how phenotypic differences arise despite relatively low genetic diversity and overall high genetic admixture among individuals. In Kenyan house sparrows, maintenance of high levels of flexibility and differential developmental influences may be important factors that lead to varying phenotypes dependent on time since colonization.

Non-native species are a major cause of endangerment for native species, but the mechanisms are often unclear. As species invasions continue to rise, it is important to understand how to mitigate this threat. Our field experiment quantified the impact of introduced Abert's squirrels (Sciurus aberti) on rates of food removal within the range of the critically endangered Mount Graham red squirrel (Tamiasciurus hudsonicus grahamensis). In the presence of Abert's squirrels, the time until 50% of cones were removed was 5.55 days faster than when Abert's squirrels were excluded (95% confidence interval 2.25 to 11.63 days). The impact on food availability as a result of cone removal by Abert's squirrels suggests the potential of food competition as a mechanism of endangerment for the Mount Graham red squirrel. Eradication of established non-native populations is rare. Management targeted at specific mechanisms by which non-natives cause endangerment is an essential tool for conservation.

Detrital subsidy from highly productive kelp beds and forests to adjacent habitats represents a major form of connectivity between coastal ecosystems that regulates regional patterns of community organization and production. In this thesis, I investigated environmental and biological factors that influence the rate of detrital production in Nova Scotian kelp beds, with emphasis on the role of invasive species in altering these dynamics. The rate of blade erosion of the dominant kelp species (Saccharina latissima and Laminaria digitata) increased significantly with the level of encrustation by the invasive bryozoan Membranipora membranacea and the extent of grazing damage by the native snail Lacuna vincta, and by increased water temperature and site exposure. The rate of detrital production (as dry mass) ranged from 0.5 to 1.71 kg m-2 y-1 across 5 sites, and increased linearly with kelp bed biomass. Spatial variation in the total level of grazing damage on kelp blades by L. vincta was explained in part by a negative relationship with site exposure, and the distribution of grazing along blades was regulated by kelp growth rate and the associated production of grazing-deterrent polyphenolics. Grazing damage by L. vincta that exceeded 0.5 to 1.0% of blade area caused increased rates of erosion during heavy wave action associated with a passing hurricane. The maximum stress before breakage, toughness, and extensibility of blade tissues decreased with the degree of encrustation by M. membranacea or grazing damage by L. vincta, which cause degradation and removal of the outer cell layers of kelp tissues, resulting in stress concentration and breakage at lower force applications than required to break undamaged tissues. The invasive green alga Codium fragile and S. latissima differed with respect to nutritional quality and changes in biochemical composition that occurred over the course of degradation on a sandy bottom adjacent to a kelp bed. Macrofaunal communities colonizing detrital deposits responded to these differences. These findings demonstrate that invasive species can alter the quantity and quality of detritus produced from subtidal kelp beds, and that their community-level effects can extend well beyond the invaded habitats via the export of detritus.

Wetland value is threatened by invasive plant species such as Reed Canarygrass (Phalaris arundinacea). Hence the research objectives of this project were to determine if reed canarygrass abundance has an effect on plant species diversity and assess the effectiveness of novel treatments on reed canarygrass control in a constructed wetland. Four treatments (mowing, herbicide, mowing plus herbicide, and a control) followed by broadcast seeding were applied to regulate growth of reed canarygrass. Principal components analysis, biodiversity measures, and ANOVA were used to identify community composition, quantify biodiversity values and identify treatment differences respectively. Results indicated differences in species composition between east and west blocks of the study site, reed canarygrass abundance appears to keep plant species diversity low, indigenous species were rare, and reed canarygrass was resistant to treatments.The results of this study are not surprising considering there is little evidence that treatments for reed canarygrass control are effective.

Ineffective biological control of the Eucalyptus pest Paropsis charybdis Stål (Coleoptera: Chrysomelidae: Paropsini) in cold areas of New Zealand was believed to be caused a climatic mismatch of the egg parasitoid Enoggera nassaui Girault (Hymenoptera: Pteromalidae). Two Tasmanian strains of the parasitoid were introduced to test climate-matching theory in 2000, with approximately 7000 wasps released. Establishment of the Florentine Valley strain was detected in 2002 using the Mitochondrial (mtDNA) gene Cytochrome Oxidase I (COI) as a strain specific marker. The hyperparasitoid Baeoanusia albifunicle Girault (Hymenoptera: Encyrtidae) and primary parasitoid Neopolycystus insectifurax Girault (Hymenoptera: Pteromalidae) were detected for the first time in New Zealand.
As paropsines have proven highly invasive internationally, a risk assessment of the paropsine threat to New Zealand was undertaken by evaluating the host range of E. nassaui and a reproductive assessment of 23 paropsine species in the genera Dicranosterna Motschulsky, Chrysophtharta Weise, Paropsis Olivier, Paropsisterna Motschulsky and Trachymela Weise. Enoggera nassaui proved polyphagous, but bioassay results proved that Paropsis species were significantly more susceptible to the egg parasitoid than Chrysophtharta species. Resistance within Chrysophtharta was attributed to spine-like chorion modifications. A COI derived Chrysophtharta phylogeny divided the genus into two distinct groupings, which was supported by chorion morphology.
Paropsine reproductive output was tested for key parameters indicating pest potential. Pest species displayed fecundity exceeding 600 eggs at an oviposition rate above 10 eggs per day⁻¹. Several non-pest species were identified as potential pests based on these parameters. The Chrysophtharta phylogeny suggested a moderate relationship between genetic relatedness and reproductive output. The Acacia defoliating paropsine Dicranosterna semipunctata (Chapuis) was evaluated for its susceptibility to E. nassaui and reproductive output. Egg parasitism occurring in bioassay did not translate into biological suppression following a specifically targeted release of E. nassaui, and the fecundity and oviposition rates fell below the thresholds predicted for a pest paropsine species.
Despite establishment of Tasmanian E. nassaui, hyperparasitism has now rendered this control agent ineffective in New Zealand. Neopolycystus insectifurax offers the best hope for future biological control of paropsine species in New Zealand.

Poison hemlock, Conium maculatum (Apiaceae), is an invasive plant in North America with a unique toxic chemistry. Previous research on this plant has focused on identifying herbivores as potential biological control agents or describing the toxic plant alkaloids. However, none have examined the role of higher trophic levels in the food web surrounding poison hemlock. Generalist predators and food web interactions are an important component of studies investigating invasion effects, as plant or animal introductions can alter ecosystem functioning. In this study, predators in poison hemlock were sampled at the foliar and epigeal levels, resulting in 956 Carabidae and 321 Coccinellidae being collected. Predator connectedness to plant resources was quantified using molecular gut-content and chemical analyses. Foliar Harmonia axyridis (Coccinellidae) contained aphid DNA and plant chemicals, while Harpalus pensylvanicus (Carabidae) only contained alkaloids, suggesting that the ground predators were obtaining plant chemicals via alternative prey. Feeding trials between H. axyridis and their potentially toxic prey, Hyadaphis foeniculi (Aphididae), revealed that the exotic predator shows faster development when consuming aphids from poison hemlock compared to alternative diets. This study reveals that three Eurasian species may be facilitating one another, illustrating the importance of continued examination of invasive species interactions.