Thesis (doctoral)--Rijks-Universiteit te Leiden, 1889. / "Stellingen": p. [175]-180. Includes bibliographical references and errata.

Thesis (MHCS(Kultuurgeskiedenis)--Universiteit van Pretoria, 2003. / Includes bibliographical references.

Orientador: Gil Eduardo Serra / Tese (doutorado) - Universidade Estadual de Campinas, Faculdade de Engenharia de Alimentos / Made available in DSpace on 2018-07-21T09:37:52Z (GMT). No. of bitstreams: 1 CesareVidaurre_TeresaJacoba_D.pdf: 4896751 bytes, checksum: 36b2186bfac3b85207eac90811a5d618 (MD5) Previous issue date: 1996 / Resumo: O presente trabalho teve como objetivo o desenvolvimento e a otimização da produção de bioinseticida bacteriano a partir do isolamento e seleção de uma linhagem de Bacillus thuringiensis que apresentasse uma diferenciada e alta atividade inseticida contra larvas de mosquitos de importância na saúde pública. A partir de amostras de solo, isolou-se uma bactéria entomopatogênica, denominada SP-36. Este isolado apresentou uma atividade de 30.767 ITU/mg, contra larvas de Aedes aegypti quase duas vezes mais alta que a linhagem padrão B. thuringiensis israelensis IPS-82, e foi classificado taxonomicamente como sendo um Bacillus thuringiensis. Na sorotipagem do isolado, este foi classificado como uma cepa autoglutinante de B.t.. O perfil electroforético das proteínas que compõem os cristais do isolado (110, 68, 38 e 25 kDa) apresentou significativa diferenças com os polipeptídios produzidos pela linhagem padrão. A otimização das condições fermentativas foram planejadas através da Metodologia por Superfícies de Resposta (MSR) com um delineamento experimental 34. Os resultados experimentais demonstraram uma resposta de 1,37 x 108 para contagem de esporos, completa lise celular e atividade de 35.000 ITU/mg, utilizando-se meio basal em condições de 30°C, 350 rpm, 5 % de inóculo e 7,2 de pH inicial. Para seleção dos componentes de meio de cultura à base de sub-produtos agro-industriais, foram utilizados fatoriais fraccionários, permitindo a seleção das fontes de carbono, nitrogênio e suplementos minerais, assim como o ajuste preliminar das concentrações dos componentes. foram selecionados os seguintes componentes: melaço, água de maceração de milho, água de levedura e fosfato de potássio. Para a otimização da concentração dos componentes selecionados utilizou.:se de delineamento central composto 54 da metodologia por superfícies de resposta, obtendo-se contagem de 1,28 x 108 esporos/ml, em 24 horas com um meio otimizado: 3,5g/l de melaço, 7,O g/l de água de maceração de milho, 16g/l de água de levedura e 6,O g/l de fosfato de potássio. Foram realizados testes de escalamento, com 30 e 50 % de oxigênio dissolvido, obtendo-se velocidades específicas de crescimento de 0,25 e.0,51 h-1, e contagem de esporos de 1,76 e 1,96 x 109 esp/ml respectivamente, em 24 e 18 horas / Abstract: The purpose of this work was to develop and improve the production of bacterial bioinsecticide from a microbiological screening and selection of a Bacillus thuringiensis strain which presents a high and differentiated insecticide activity against mosquitoes larvae of public heath importance. The entomopathogenic bacteria B.t. strain SP-36, has been isolated from soil samples, according to serotyping it as an auto-agglutinated B.t. strain. This isolate presented an activity of 30.767 ITU/mg against Aedes aegypti larvae, almost twice as high as the B. thuringiensis israelensis IPS-82 standard strain. The electrophoretic pattern of the proteins, which constitute the isolate crystals (110, 68, 38 and 25 kDa), has presented significant differences as to the polypeptides produced by the standard strain. The improvement of the fermentative conditions has been planned through the Response Surfaces Methodology (MSR) with an experimental design 34. The experimental results have shown a response of 1,37 x 108 for the spore count, complete cellular lysis and an activity of 35.000 ITU/mg, using a basal medium at 30°C, 350 rpm, 5% inoculum and initial pH 7,2. In order to select the culture medium based on agroindustrial by-products, fractional fatorials have been used, so that the sources of carbon, nitrogen and mineral suplements could be selected, as well as the preliminary setting of the components concentration. The following components have been selected: sugarcane molasses, com-steep liquor, yeast liquor and potassium phosphate. In order to improve the selected components concentration a central composite design 54 of the response surfaces methodology was used, obtaining a count, of 1,28 x 108 spores/ml, in 24 hours, in an optimized medium: 3,5g/l of molasses, 7,0g/l of com-steep liquor, 16g/l of yeast liquor and 6,0g/l of potassium phosphate. Scale-up tests have been performed, with 30 and 50% dissolved oxygen, obtaining specific growth rate of 0,25 and 0,51 h-1 and spores count of 1,76 and 1,96 x 109 spores/ml, in 24 hours, respectively / Doutorado / Doutor em Tecnologia de Alimentos

In her lifetime Jacoba Elizabeth (Nonnie) de la Rey, née Greeff (1856-1923) was widely known not only for being the wife of the eminent General Koos de la Rey, but also for her exceptional tenacity during the Second Anglo-Boer War (1899-1902). From December 1900 to the conclusion of the war in May 1902 she and her children lived a nomadic life. Nonnie's experiences were recorded in her memoirs, Mijne omzwervingen en beproevingen gedurende den oorlog, which were published in Amsterdam in 1903. Shortly thereafter – also in 1903 – the English edition, A Woman's Wanderings and Trials During the Anglo-Boer War, as translated by Lucy Hotz, was published in Londen. The 18 months that Nonnie spent wandering through the country probably constitute the most important phase of her life, but she experienced a great deal more, which she recorded in another memoir. Her childhood and pioneer's life are well documented. At the age of eighteen Nonnie met Jacobus Herculaas de la Rey; they were married on 24 October 1876 and settled in the vicinity of Lichtenburg. The period from 1880 to 1899 constitute a kaleidoscope of events in Nonnie's life, for example the First Anglo-Boer War (1880-1881), the loss of Nonnie's father and mother, and a move to the farm Elandsfontein. The South African War broke out in 1899. Nonnie's life during the war years can be divided into two phases, namely the period when she lived in Lichtenburg (October 1899 to November 1900), and the period characterised as her wandering years (December 1900 to May 1902). Events in Nonnie de la Rey's life from 1902 to 1923 include their official visit to Europe, the restoration of Elandsfontein, Union (1910) and Koos de la Rey's political involvement, the Rebellion of 1914 and the death of Koos de la Rey. In her final years Nonnie was involved with various organisations. She was an eminent presence in her community, being revered as a “volksmoeder” by her compatriots. She died in 1923. / Dissertation (MHCS)--University of Pretoria, 2005. / Historical and Heritage Studies / unrestricted

A museum has to care for the objects in its collection to the best of its ability. The concept collections management emerged in the 1960s, when accountability for collections became a strong incentive for museums to develop modern collections management practices. In the process of establishing accountability (the effective implementation of practices to ensure adherence to collections policies on the accessioning, care and disposal of objects in a museum collection) many museums encountered problems such as the lack of access to detailed information about the objects in collections, a proliferation of accession numbers and inadequate location control. These problems were also encountered at the National Cultural History Museum, Pretoria. This research reveals the way in which the historical, anthropological and archaeological collections at the Transvaal Museum, predecessor of the National Cultural History Museum were managed from 1913 to 1964. This period was chosen for the following reasons: -- J W B Gunning, the director of the Transvaal Museum, was succeeded by H G Breijer in 1913. The year 1913 is thus a clear starting point for research and a new beginning, a watershed, at the Museum. -- The year 1964 marked the inception of an autonomous museum, the National Cultural History and Open-Air Museum, and the discontinuance of responsibility, after a period of 60 years, for the anthropology, archaeology and history collections at the Transvaal Museum. The development of the Transvaal Museum as a natural history and a history museum, is traced. In 1953, for the first time, a trained professional officer was appointed for the history division at the Museum. After 1953 there was an increased awareness (from a professional point of view) that historical, anthropological and archaeological collections require specialized curatorial care. Modern collections management principles, although they were not called by this name, featured effectively in the handling of the historical collection in particular, for the first time in more than 50 years. Aspects such as departmental organization, the staff, expansion of collections, policies, documentation and conservation are investigated. An evaluation of the factors that played a decisive role in collections management practices for the historical, anthropological and archaeological collections shows that a combination of aspects has to be considered in order to understand the practices that were followed and the changes that were made. / Thesis (DPhil (Museology))--University of Pretoria, 2006. / Historical and Heritage Studies / unrestricted

The diploma thesis deals with seven novels by the contemporary British novelist David Mitchell. Using textual analysis, it traces the ways in which the novels are connected, most visibly expressed through reappering characters and realities, and provides an overview of Mitchell's biblioverse. The work also notes the differences between the fictional and the real, which Mitchell's novels examine, and leads to a broader reflection on the meaning of storytelling or the meaning of a cultural encyclopedia.