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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

ESTABLISHMENT OF A QUISCENCE HERPES SIMPLEX TYPE 1 INFECTION IN L929 FIBROBLASTS AND NEURO-2A CELLS BY A NUCLEOSIDE ANALOGUE ACYCLOVIR

Shaklawoon, Noura January 2013 (has links)
No description available.
2

Establishment of a Quiescent Infection of HSV-1 in L929 Fibroblasts using a Mitotic Inhibitor and IFN-γ

Shinde, Neelam V. 17 April 2012 (has links)
No description available.
3

Detection of structural changes based on Mie-scattering analyses of mouse fibroblast L929 cells before and after necrosis

Baselt, Tobias, Richter, Clemens, Rudek, Florian, Nelsen, Bryan, Lasagni, Andrés Fabián, Hartmann, Peter 13 August 2020 (has links)
The aim of the presented work is to investigate the angle-resolved scattering characteristics of biological nano- and micro-scaled cell structures. The scattering results of cellular structures were compared to measurements of ideal spherical nano- and micro-particles. A monolayer of mouse fibroblasts L929 cells was cultivated in a Dulbecco's Modified Eagle Medium (DMEM) in a standard 24 well cell culture plate. The system allows an in situ measurement directly in the standard cell culture plate and a contaminant-free investigation of the viability of the cell cultures. Of particular interest was whether changes in the tumor characteristics occur in necrosis or other cell-harming effects. Because of the size ratios between wavelength and the scattering particles, all observations were investigated using Mie scattering theory. A setup for reliable measurements was developed and the scattered angle dependent intensity obtained was compared with simulated scattering characteristics. A homemade supercontinuum (SC) light source was filtered by an optical bandpass filter with a central wavelength of 500 nm. The scattered portion of the pulsed SC light behind the sample was recorded in a time-resolved manner at defined angles. A specimen holder adapted to standard cell culture plates allows detection of scattered radiation at angles between ±80° without angle-dependent Fresnel reflection losses and a Snell’s law bending of the propagation direction. Finally, the system was tested to detect structural changes of mouse fibroblast L929 cells before and after poisoning the cells with the cell detergent Triton X100 and the data clearly shows changes in the scattering characteristics when the cells were destroyed.
4

Toxicidade da clorexidina injetada na pata de camundongos e adicionada em cultura de fibroblastos L929 / Chlorhexidine toxicity injected in the paw of mice and added to cultured L929 fibroblasts

Faria, Gisele 04 June 2007 (has links)
Como a clorexidina (CHX) tem sido recomendada como solução irrigadora de canais radiculares e como curativo de demora, o objetivo do presente estudo foi caracterizar in vivo a lesão induzida pela injeção de CHX a 0,125, 0,25, 0,5 e 1,0% na pata de camundongos em intervalos de tempo selecionados (24 e 48 horas e 7 e 14 dias) e in vitro o modo e a causa morte celular (necrose e/ou apoptose) e o estresse causado pela exposição de fibroblastos L929 em cultura a concentrações crescentes da droga (0,000125, 0,00025, 0,0005, 0,001, 0,002, 0,004, 0,008 e 0,016%) por 24 horas. A proliferação celular foi avaliada por meio da incorporação de metil-3H-timidina ao DNA das células e imunomarcação para PCNA. A ultraestrutura foi analisada em microscópio eletrônico de transmissão e de varredura e o citoesqueleto das células por meio de marcação para actina e α-tubulina. Citometria de fluxo (Anexina-V FITC/iodeto de propídeo) foi empregada para diferenciar células necróticas de apoptóticas. Também, foi efetuada marcação para retículo endoplasmático, Bcl-2 (B-célula CLL/linfoma 2), Hsp70 (proteína de choque térmico 70) e Grp78 (glucose-regulated protein 78). Quando injetada no espaço subplantar da pata traseira de camundongos, a CHX induziu alterações necróticas na epiderme, derme e tecido subcutâneo em associação com uma resposta inflamatória reacional, particularmente nas concentrações mais elevadas. Em cultura de fibroblastos, a CHX induziu a diminuição da proliferação celular, causou morte celular por apoptose e necrose, desestruturação do citoesqueleto, alteração da morfologia celular, aumento da área das células, dilatação do retículo endoplasmático rugoso e acúmulo de proteínas nas cisternas. Além disso, a CHX causou aumento da expressão de Hsp70, de Grp78 (indicadores de estresse celular) e de Bcl-2 (proteína anti-apoptótica). Em conclusão, a CHX injetada no espaço subplantar da pata traseira de camundongos induz efeitos tóxicos severos. Além disso, a CHX adicionada em cultura de fibroblastos causou estresse do retículo endoplasmático como consequência do acúmulo de proteínas nas cisternas e induziu a morte celular por necrose e apoptose via estresse do retículo endoplasmático, além de causar estresse celular. Os resultados sugerem que a CHX poderia ter um efeito desfavorável na resolução de lesões periapicais em decorrência de sua ação tóxica sobre as células do tecido em torno do ápice dentário. / Because chlorhexidine (CHX) has been recommended as either endodontic irrigant or root canal dressing, this study aimed to characterize in vivo the lesion induced by injection of CHX at concentrations of 0.125, 0.25, 0.5 and 1.0% in the paw of mice at selected time intervals (24 and 48 hours and 7 and 14 days) and in vitro the mode and the cause of cell death (necrosis and /or apoptosis), and the cellular stress caused by exposition of cultured L929 fibroblasts to ascending concentrations of the drug ( 0.000125, 0.00025, 0.0005, 0.001, 0.002, 0.004, 0.008 and 0.016%) for 24 hours. The cell proliferation assay was performed by measuring incorporation of metil-3H-thymidine to cell DNA and immunocytochemical staining analysis of PCNA. The cell ultrastructure was analyzed in transmission and scanning electron microscopes and the cell cytoskeleton by florescence analysis of actin and α- tubulin. Apoptosis and necrosis were discriminated by flow cytometry with annexin V-FITC and PI. In addition endoplasmic reticulum, Bcl-2 (B-cell CLL/lymphoma 2), Hsp70 (heat shock protein 70) and Grp78 (glucoseregulated protein 78) were detected by fluorescence. CHX injected in the sub plantar space of the hind paw of mice induced necrotic changes in the epidermis, dermis and subcutaneous tissue in association with reactive inflammatory response, particularly at higher concentrations. In cultured fibroblasts, CHX decreased cellular proliferation, caused cell death by apoptosis and necrosis, disruption of the cytoskeleton, morphological cellular alterations, increased cells size (area), rough endoplasmic reticulum dilatation with accumulation of cysternal protein. In addition, CHX induced increased expression of Hsp 70, Grp78 (indicators of cellular stress) and Bcl-2 (anti-apoptotic protein). It was concluded that CHX injected in the sub plantar space of the hind paw of mice could induce severe toxic effect. In addition CHX caused endoplasmic reticulum stress as a consequence of accumulation of proteins in the endoplasmic reticulum cysterns and induced cell death by apoptosis and necrosis via endoplasmic reticulum stress and caused cellular stress. Taken together, these findings suggest that CHX may have an unfavorable effect on the resolution of apical periodontitis, due a toxic effect on the periapical tissue cells.
5

Toxicidade da clorexidina injetada na pata de camundongos e adicionada em cultura de fibroblastos L929 / Chlorhexidine toxicity injected in the paw of mice and added to cultured L929 fibroblasts

Gisele Faria 04 June 2007 (has links)
Como a clorexidina (CHX) tem sido recomendada como solução irrigadora de canais radiculares e como curativo de demora, o objetivo do presente estudo foi caracterizar in vivo a lesão induzida pela injeção de CHX a 0,125, 0,25, 0,5 e 1,0% na pata de camundongos em intervalos de tempo selecionados (24 e 48 horas e 7 e 14 dias) e in vitro o modo e a causa morte celular (necrose e/ou apoptose) e o estresse causado pela exposição de fibroblastos L929 em cultura a concentrações crescentes da droga (0,000125, 0,00025, 0,0005, 0,001, 0,002, 0,004, 0,008 e 0,016%) por 24 horas. A proliferação celular foi avaliada por meio da incorporação de metil-3H-timidina ao DNA das células e imunomarcação para PCNA. A ultraestrutura foi analisada em microscópio eletrônico de transmissão e de varredura e o citoesqueleto das células por meio de marcação para actina e α-tubulina. Citometria de fluxo (Anexina-V FITC/iodeto de propídeo) foi empregada para diferenciar células necróticas de apoptóticas. Também, foi efetuada marcação para retículo endoplasmático, Bcl-2 (B-célula CLL/linfoma 2), Hsp70 (proteína de choque térmico 70) e Grp78 (glucose-regulated protein 78). Quando injetada no espaço subplantar da pata traseira de camundongos, a CHX induziu alterações necróticas na epiderme, derme e tecido subcutâneo em associação com uma resposta inflamatória reacional, particularmente nas concentrações mais elevadas. Em cultura de fibroblastos, a CHX induziu a diminuição da proliferação celular, causou morte celular por apoptose e necrose, desestruturação do citoesqueleto, alteração da morfologia celular, aumento da área das células, dilatação do retículo endoplasmático rugoso e acúmulo de proteínas nas cisternas. Além disso, a CHX causou aumento da expressão de Hsp70, de Grp78 (indicadores de estresse celular) e de Bcl-2 (proteína anti-apoptótica). Em conclusão, a CHX injetada no espaço subplantar da pata traseira de camundongos induz efeitos tóxicos severos. Além disso, a CHX adicionada em cultura de fibroblastos causou estresse do retículo endoplasmático como consequência do acúmulo de proteínas nas cisternas e induziu a morte celular por necrose e apoptose via estresse do retículo endoplasmático, além de causar estresse celular. Os resultados sugerem que a CHX poderia ter um efeito desfavorável na resolução de lesões periapicais em decorrência de sua ação tóxica sobre as células do tecido em torno do ápice dentário. / Because chlorhexidine (CHX) has been recommended as either endodontic irrigant or root canal dressing, this study aimed to characterize in vivo the lesion induced by injection of CHX at concentrations of 0.125, 0.25, 0.5 and 1.0% in the paw of mice at selected time intervals (24 and 48 hours and 7 and 14 days) and in vitro the mode and the cause of cell death (necrosis and /or apoptosis), and the cellular stress caused by exposition of cultured L929 fibroblasts to ascending concentrations of the drug ( 0.000125, 0.00025, 0.0005, 0.001, 0.002, 0.004, 0.008 and 0.016%) for 24 hours. The cell proliferation assay was performed by measuring incorporation of metil-3H-thymidine to cell DNA and immunocytochemical staining analysis of PCNA. The cell ultrastructure was analyzed in transmission and scanning electron microscopes and the cell cytoskeleton by florescence analysis of actin and α- tubulin. Apoptosis and necrosis were discriminated by flow cytometry with annexin V-FITC and PI. In addition endoplasmic reticulum, Bcl-2 (B-cell CLL/lymphoma 2), Hsp70 (heat shock protein 70) and Grp78 (glucoseregulated protein 78) were detected by fluorescence. CHX injected in the sub plantar space of the hind paw of mice induced necrotic changes in the epidermis, dermis and subcutaneous tissue in association with reactive inflammatory response, particularly at higher concentrations. In cultured fibroblasts, CHX decreased cellular proliferation, caused cell death by apoptosis and necrosis, disruption of the cytoskeleton, morphological cellular alterations, increased cells size (area), rough endoplasmic reticulum dilatation with accumulation of cysternal protein. In addition, CHX induced increased expression of Hsp 70, Grp78 (indicators of cellular stress) and Bcl-2 (anti-apoptotic protein). It was concluded that CHX injected in the sub plantar space of the hind paw of mice could induce severe toxic effect. In addition CHX caused endoplasmic reticulum stress as a consequence of accumulation of proteins in the endoplasmic reticulum cysterns and induced cell death by apoptosis and necrosis via endoplasmic reticulum stress and caused cellular stress. Taken together, these findings suggest that CHX may have an unfavorable effect on the resolution of apical periodontitis, due a toxic effect on the periapical tissue cells.
6

In-vitro-Untersuchung der antimikrobiellen und zytotoxischen Eigenschaften eines kupferhaltigen Zinkoxidphosphatzementes / In vitro antimicrobial and cytotoxic properties of a phosphate cement with copper additive

Wassmann, Torsten 15 November 2017 (has links)
No description available.
7

Die Reorganisation des Aktinzytoskeletts in Hypoxie: Neue Erkenntnisse über die Rolle von ArhGAP29 / Remodeling of the actin cytoskeleton in hypoxia: An emerging role for ArhGAP29

Peters, Johannes 22 August 2019 (has links)
No description available.
8

Trajectoires d'enfants de la rue d'Haïti ayant bénéficié d'une intervention d'une ONG visant l'insertion sociale : que sont devenus ces enfants?

Lubin, Irdèle 12 April 2018 (has links)
Depuis plus de deux décennies, Haïti, comme plusieurs pays du monde est confronté au problème d'enfants et de jeunes qui laissent leur environnement familial pour se réfugier dans la rue. Ces enfants sont à la recherche d'un mieux être que la famille ne peut pas ou ne peut plus leur offrir. Qui sont ces enfants? Pourquoi se trouvent-ils dans la rue? À combien estime t-on leur nombre? Cette étude a permis d'obtenir des réponses à ces questions et à tant d'autres encore. Depuis plus de vingt ans, diverses interventions se pratiquent auprès de ces enfants par des organisations non gouvernementales notamment. Celles-ci disent viser la réinsertion familiale, sociale et économique au sein de la société haïtienne. Que sont devenus ces enfants de la rue en Haïti qui ont bénéficié d'interventions des ONG au cours de la période allant de 1986 à 1999? Plus spécifiquement, qu'est-il advenu de ces enfants sur le plan de leur insertion sociale (communautaire, familiale et économique)? Cette recherche vise à apporté un éclairage à ces questions. Cette recherche, de type qualitatif, est réalisée à partir de l'histoire de vie de 25 jeunes, 18 hommes et 7 femmes, âgés de 18 à 34 ans. Ils ont été dans les rues de Port-au-Prince de 1986 à 1996. Ils ont été rejoints par la technique boule de neige. Le travail de terrain s'est déroulé à Port-au-Prince au cours de la période allant de juin 2004 à juillet 2005. Les résultats permettent d'avancer que ces enfants viennent des zones rurales ou des régions pauvres de Port-au-Prince. Ils ont tous vécu dans une famille mono ou biparentale, ils n'ont pas été rejetés par celle-ci. Les raisons qui les ont poussés dans les rues sont de deux ordres : un déclencheur et un profond. Trois catégories de jeunes sont identifiés à partir des résultats: ceux qui ont abandonné complètement la rue a leur sortie des centres, ceux qui sont retournés vivre dans la rue après leur passage au centre et une dernière catégorie qui mène une vie partagée entre ces deux milieux. / En las ciudades de Haiti, como en varias otras de muchos paises del mundo, el problema de los ninos de la calle sigue siendo un reto desde el ano 1986. Varios ninos y ninas abandonan su hogar para ir deambulando en la calle, buscando algo que la familia no puede o ya no puede ofrecerles. ¿ Quienes son estos ninos? ¿ Cuantos son? ¿ Porque van a la calle? La présente investigaciôn permite contestar estas preguntas. Varias organizaciones no gubernamentales -ONG- ayudan estos ninos para que dejen de vivir en la calle. Trabajan con estos ninos y ninas desde mâs de veinte anos. Propusieron reintegrarlos en la sociedad después de su intervenciôn. ¿ Que ha pasado con los que estuvieron en estas instituciones? De manera especifica, ?Que ocurriô con su integraciôn comunitaria, familial y econômica? Esta investigaciôn trae algunas respuestas. Esta investigaciôn es de tipo cualitativo. Lleva sobre 25 personas: 18 hombres y 7 mujeres. Tienen entre 18 y 34 anos de edad. Estuvieron todos en centros de atenciôn para ninos de la calle. Los encontremos por la técnica boule de beige. Los resultados permiten decir que vienen en gran parte de zonas rurales y de las capas mâs pobres del pais. Los dividimos en très categorias. La primera déjà de vivir en la calle; la segunda se encuentra todavia en la calle a pesar de su estancia en un programa de una ONG; la tercera vive entre la calle y una casa. ¿ / Since last past twenty years, cities in Haïti, like many others in the world, had encountered the phenomenon of street children and street youth. Many children leave their family environment to go the street. Who are these children? How many they are? What they go out of their family? This research gives the responses to these questions. Many ONGs try to help these children. They want their reinsertion to the Haitian society before leaving the institution. Since the beginning of the intervention of these ONGs on this problem in 1986, there is no information concerning these children who where on the street and who leave in these institutions between 1986 of 1999. What about these children? Specifically, what about their community, social and economic reinsertion in Haitian society? This research gives the responses to these questions. This study is a qualitative research concerning 25 life histories of young adult aged to 18 to 34 years who where living in the street between 1986 to 1996. These young adult have to pass in the program of an ONG intervention between four months at least. They are 18 men and 7 women who had joined by boule de neige. The investigation has realized on June 2004 to July 2005 in Port-au-Prince, the capital city of Haiti. The results find that these young adults origin to the rural areas or to the area where are many poverty in the country. They leaved whit their family before going to the street. Too fundamentals reasons push them to the street une déclencheur et une profonde. These children can divide in tree catégories: the first one leave out of the street after their life in the ONG ; the second return to the street and the third continue their life between street and home..
9

Wirkung physiko-chemischer Oberflächencharakteristika auf Zytotoxizität verschiedener dentaler Komposite / Effect of physicochemical surfaces Characteristics of cytotoxicity of various dental composites

Kurbad, Oliver 16 May 2019 (has links)
No description available.
10

Kompozitní stomatologické biomateriály - struktura, analýza a vlastnosti / Composite Dental Biomaterials - Structure, Analysis and Properties

Matoušek, Aleš January 2012 (has links)
The aim of this work is to define relations between grain size and bioaktivity of oxide ceramics, specifically ZrO2, Al2O3 and HA. Ceramic materials with grain size from 100 nm up to 10 m, with various surface roughness, were tested for its bioactivity. Ceramography analysis was performed for all tested materials to precisely describe microstructures. Biological properties of the ceramic materials were tested via dilation tests directly in-vitro and by in-vitro extraction. Three cell culturing lines: osteoblast MG63, fibroblast L929, and epithelioid HeLa, were used for our testing. An influence of the grain size on the biological response was only found for the ceramic materials which had been thermally etched. The thermally etched nanocrystalline samples had larger areas covered by cells than ceramics with coarse grain microstructure. Biological tests on layered composites Al2O3×ZrO2 showed the cell selection determined by the type of material, where ZrO2 surfaces were preferably covered. Improved biological response of nanocrystalline ZrO2 was demonstrated on ceramic ZrO2, Al2O3 and SiO2 substrates with nanocrystalline coating of ZrO2. In this work a novel technological process for the formation of defect-free coatings was developed. Sintered coatings were tested using in-vitro technique with cell line HeLa, L929 and MG63 for up to 72 hours. The results of the biological tests of nanocrystalline coatings were consistent with results from the bulk nanocrystalline thermally etched ZrO2 ceramics.

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