Escherichia coli O157:H7 and Salmonella have emerged as the most common foodborne enteric pathogens causing human illness from the consumption of beef. By mandate of the U.S. Department of Agriculture (USDA), Food Safety and Inspection Service (FSIS), the industry has implemented a Hazard Analysis and Critical Control Points (HACCP) system that utilize intervention technologies for controlling, preventing, and/or reducing enteric pathogens. In addition, USDA-FSIS has mandated that each facility must validate, monitor, and verify the effectiveness of each intervention implemented to eliminate E. coli O157:H7 and Salmonella. For this study, microbial decontamination interventions at two beef slaughter facilities were validated to demonstrate effectiveness in eliminating or reducing enteric pathogens. The facilities selected utilized either a lactic acid spray treatment or a combination of hot water followed by a lactic acid treatment. At both facilities, mesophilic plate counts (MPC) were significantly (P < 0.05) reduced, and E. coli and coliforms were eliminated below detectable limits at both facilities. No Salmonella positive samples were detected after either facility's intervention sequence. The framework used in this research to validate interventions can also be utilized in the future for yearly verification of the effectiveness of each intervention.

Thesis (Ph.D.)--Ohio State University, 2003. / Title from first page of PDF file. Document formatted into pages; contains xiv, 182 p.; also includes graphics. Includes abstract and vita. Advisor: Ahmed E. Yousef, Dept.of Food Science and Nutrition. Includes bibliographical references (p. ).

Abstract Lactate utilising bacteria (LUB) assist in reducing the risk of ruminal lactate acidosis when high concentrate diets are fed to feedlot cattle. Ruminal lactate acidosis can lead to lower animal performance and morbidity. Preliminary studies suggested that the strain, Megasphaera elsdenii (M.e.) NCIMB 41125, is a particularly potent LUB. The potential of M.e.NCIMB 41125 to improve the health and performance of feedlot cattle was investigated. Four hundred and forty eight Bonsmara steers (ca. 240 kg) were used in a 100-day feeding trial. Half the steers received at processing 200 mL M.e.NCIMB 41125 per os (LY) and the other half no LUB (LN). The diets in each of these treatments were divided into a low roughage (2%) (RL) and high roughage (8%) (RH) diet. The effects of LY vs. LN, RL vs. RH as main effects and their respective interactions (LYRL, LYRH, LNRL & LNRH) were compared in terms of morbidity, feedlot performance and carcass characteristics. The steers were weighed at two week intervals, feed was offered daily and the orts were removed weekly from each pen. The faecal consistency score and incidence of morbidity were recorded. At slaughter, carcass data were collected and the health status of the liver and rumen epithelium was assessed. Steers dosed with M.e.NCIMB 41125 had a 5.6% better average daily gain (ADG) during weeks 3 - 5 (2.09 kg/day vs. 1.98 kg/day for LY and LN, respectively). Feed conversion ratio (FCR, Weeks 1 - 13) was better for the steers fed the RL than the RH treatment (4.72 kg/kg vs. 4.99 kg/kg for RL and RH, respectively). Steers on the LNRH treatment during weeks 3 - 5 used more feed per kg gain than steers on the other treatments (5.39 kg/kg for LNRH vs. 4.74 kg/kg and 4.72 kg/kg for LYRL and LNRL, respectively). More steers (21) on the LNRL treatment were treated for morbidity than on the other treatments (8, 7 and 5 for LYRL, LYRH and LNRH, respectively). In general, animal performance was not improved by dosing with M.e.NCIMB 41125, but since ADG was improved in the immediate postadaptation phase (weeks 3 - 5) and morbidity levels were lower on the low roughage diet, dosing of steers on low roughage, lactate acidosis-prone, diets with M.e.NCIMB 41125 should prove useful.

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Leucocin A is a class 11a bacteriocin produced by Leuconostoc mesenteroides TA33a that was previously shown to inhibit Listeria monocytogenes. A spontaneous resistant mutant of L. monocytogenes was isolated, and found to be resistant to leucocin A at levels in excess of 2 mg/ml. The resistant mutant had an eight-fold increased binding capacity for leucocin A in comparison to the parental strain. The mutant showed no significant cross resistance to nisaplin or ESFI-7GR. The resistant phenotype had a similar growth rate in monoculture, to the sensitive phenotype. DNA and protein analysis of the resistant and susceptible strains were carried out using silver stained amplified fragment length polymorphism (ssAFLP) and one and two dimensional (2D) SDS polyacrylamide gel electrophoresis (PAGE). Two-dimensional SDS PAGE revealed two differences. The first was a 35 kDa protein which was present in the sensitive but absent from the resistant phenotype and, secondly there was a higher level of expression of a 18 kDa protein in the resistant phenotype compared with the sensitive phenotype. The 35 kDa protein was found to have a 83% homology to the mannose-specific phosphotransferase system IIAB of Streptococcus salivarius. / Thesis (M.Sc.)-University of Natal, Pietermaritzburg, 2000.

Thesis (M.S.)--Oregon State University, 2008. / Printout. Includes bibliographical references (leaves 37-40). Also available on the World Wide Web.

Thesis (Ph.D.)--University of Adelaide, Dept. of Horticulture, Viticulture & Oenology, 1999. / Bibliography: leaves 200-214.

Thesis (Ph. D.)--University of Hong Kong, 2009. / Includes bibliographical references (leaves 142-172) Also available in print.

Thesis (Master)--İzmir Institute of Technology, İzmir, 2005 / Keywords: Lactococcus lactis ssp. lactis, chromosome profiling, pulsed field gel electrophoresis, plasmid profiling, plasmid stability. Includes bibliographical references (leaves 58-63)