Biserrula pelecinus L. has been introduced to Australia from the Mediterranean region, in the last decade due to many attractive agronomic features. This deep rooted, hard seeded, acid tolerant and insect resistant legume species provides high quality food for cattle and sheep, and grows well under the harsh edaphic and environmental conditions of Australia. In 1994, B. pelecinus was introduced to a site in Northam, Western Australia where there were no native rhizobia capable of nodulating this legume. The introduced plants were inoculated with a single inoculant strain of Mesorhizobium sp., WSM1271. This study investigated whether a diversity of rhizobia emerged over time. A second objective was to investigate the possible mechanisms involved in the diversification of rhizobia able to nodulate B. pelecinus. Eighty eight isolates of rhizobia were obtained from nodules on B. pelecinus growing at the Northam site in August 2000, six years after introduction. These plants were self-regenerating offspring from the original seeds sown. Molecular fingerprinting PCR with RPO1 and ERIC primers revealed that seven strains (novel isolates) had banding patterns distinct from WSM1271 while 81 strains had similar banding patterns to WSM1271. A 1400 bp internal fragment of the 16S rRNA gene was amplified and sequenced for four of the novel isolates (N17, N18, N45 and N87) and WSM1271. The phylogenetic tree developed using these sequences clustered the novel isolates in Mesorhizobium. There were >6 nucleotide mismatches between three of the novel isolates (N17, N18, N87) and WSM1271 while there were 23 nucleotide mismatches between N45 and WSM1271. When B. pelecinus cv. Casbah was inoculated with the novel isolates, five (N17, N18, N39, N46 and N87) yielded <40% of the shoot dry weight of the plants inoculated with the original inoculant (WSM1271). Novel isolates N15 and N45 were completely ineffective on B. pelecinus cv. Casbah. Physiological experiments to test the ability of the novel isolates and WSM1271 to grow on 14 different carbon sources (N acetyl glucosamine, arabinose, arbutine, dulcitol, β-gentiobiose, lactose, maltose, melibiose, D-raffinose, saccharose, L-sorbose, D-tagatose, trehalose and D-turanose) as the sole source of carbon, intrinsic resistance to eight different antibiotics (ampicillin, chloramphenicol, gentamicin, kanamycin, nalidixic acid, spectinomycin, streptomycin and tetracycline) and pH tolerance (pH 4.5, 5.0, 7.0, 9.0) revealed that the novel isolates had significantly different carbon source utilization patterns to WSM1271. However, pH tolerance and intrinsic resistance to antibiotics were similar between the novel isolates and WSM1271 except for streptomycin (100 μg/ml). Novel isolates N17, N18, N46 and N87 were susceptible for this antibiotic while the other novel isolates and WSM1271 were resistant. Host range experiments were performed for the novel isolates N17, N18, N45, N87, WSM1271 and two other root nodule bacteria (RNB) previously isolated from B. pelecinus growing in the Mediterranean region (WSM1284 and WSM1497) for twenty one legumes (Amorpha fruticosa, Astragalus adsurgens, Astragalus membranaceus, Astragalus sinicus, Biserrula pelecinus cv Casbah, Dorycnium hirsutum, Dorycnium rectum, Glycyrrhiza uralensis, Hedysarum spinosissimum, Leucaena leucocephala, Lotus corniculatus, Lotus edulis, Lotus glaber, Lotus maroccanus, Lotus ornithopodioides, Lotus parviflorus, Lotus pedunculatus, Lotus peregrinus, Lotus subbiflorus, Macroptilium atropurpureum, and Ornithopus sativus). Only isolate N17 have the same host range as WSM1271 in that they both nodulated B. pelecinus and A. membranaceus, while the other three novel isolates, WSM1284 and WSM1497 had a broader host range than WSM1271. Three isolates N18, N45 and N87 formed small white nodules on M. atropurpureum, in addition to nodulating the above hosts. Isolates N18 and N45 also nodulated A. adsurgens while N45 was the only isolate to nodulate L. edulis. Isolate N87 was the only isolate to nodulate A. fruticosa. WSM1497 nodulated A. adsurgens, A. membranaceus, B. pelecinus and L. corniculatus while WSM1284 was a promiscuous strain that nodulated 16 host species out of the 21 tested. A 710 bp internal region of nifH, a 567 bp internal region of nodA and a 1044 bp internal region of intS were sequenced for N17, N18, N45, N87 and WSM1271. The sequence comparison showed that the sequences of the above three genes of the four novel isolates were identical to that of WSM1271. Eckhardt gel electrophoresis revealed that WSM1271, three other RNB isolates from B. pelecinus from the Mediterranean region and isolate N18 each have a plasmid of approximately 500 kb while N17, N45 and N87 are plasmid free. Probing of the plasmid DNA from the Eckhardt gel with nifH and nodA probes indicated that these two genes were not located on the plasmid. Furthermore, the results of this study demonstrated that 92% of the nodules on B. pelecinus growing in the Northam site six years after the introduction of this plant were occupied by the inoculant strain and the N2 fixation efficiency of the progeny strains of WSM1271 remain similar to the mother culture. This study also showed that the carbon source utilization pattern, intrinsic antibiotic resistance and pH range of the progeny strains of WSM1271 remain relatively similar, except for few variations in carbon source utilization patterns. This thesis clearly demonstrated that phenotypicaly, genetically and phylogenetically diverse strains capable nodulating B. pelecinus evolved through symbiotic gene transfer from the inoculant strain to other soil bacteria within six years. The presence of intS, and the evidence of gene transfer between these Mesorhizobium strains indicates that transfer of symbiotic genes may have occurred via a symbiosis island present in WSM1271.

Thesis (Ph.D.)--Murdoch University, 2005. / Thesis submitted to the Division of Science and Engineering. Bibliography: leaves 184-207.

Thesis (Ph. D.)--University of Wisconsin--Madison, 1963. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 143-153).

Thesis (Ph. D.)--Washington State University, August 2009. / Title from PDF title page (viewed on Aug. 28, 2009). "Department of Crop and Soil Sciences." Includes bibliographical references.

Thesis (M.S.)--Oregon State University, 2010. / Printout. Includes bibliographical references (leaves 64-72). Also available on the World Wide Web.

Thesis (M.Anim.Sc) - University of Queensland, 2005. / Includes bibliography.

SOUSA, Bruno Lopes de.Caracterização estrutural das formas silvestre e recombinante de uma lectina de sementes de Vatairea macrocarpa Benth e análise das suas bases moleculares de ligação ao antígeno Tn. 2014. 119 f. Tese (Doutorado em Bioquímica)-Universidade Federal do Ceará, Fortaleza-CE, 2014. / Submitted by Eric Santiago (erichhcl@gmail.com) on 2016-07-20T12:14:08Z No. of bitstreams: 1 2014_tese_blsousa.pdf: 4344064 bytes, checksum: 784bc6294f0c6ff7014ef6c5ecece099 (MD5) / Approved for entry into archive by José Jairo Viana de Sousa (jairo@ufc.br) on 2016-08-02T20:33:39Z (GMT) No. of bitstreams: 1 2014_tese_blsousa.pdf: 4344064 bytes, checksum: 784bc6294f0c6ff7014ef6c5ecece099 (MD5) / Made available in DSpace on 2016-08-02T20:33:39Z (GMT). No. of bitstreams: 1 2014_tese_blsousa.pdf: 4344064 bytes, checksum: 784bc6294f0c6ff7014ef6c5ecece099 (MD5) Previous issue date: 2014 / Lectins are a very diverse class of proteins able to bind specific sugar structures reversibly and with high specificity, but without enzymatically modifying them, triggering several important cellular processes. Among the different lectin families, legume lectins are the most thoroughly studied and have been widely reported to exhibit a number of links to many pathological processes, including carcinogenesis. The remarkable anti-tumor properties of some legume lectins, resulting from their ability to induce programmed cell death and/or autophagocytosis in cancer cells have attracted much attention for biomedical applications. Moreover, a few galactose/N-acetylgalactosamine (Gal/GalNAc)-binding lectins from this group have proven to be useful markers for cancer histochemistry, and the structural characterization of these lectins bound to specific cancer epitopes has been carried out successfully. The seed lectin isolated from the legume tree Vatairea macrocarpa (VML) is a well characterized Gal/GalNAc-binding protein able to specifically recognize naturally occurring O-mucins presenting the carcinoma epitope Tn antigen (GalNAc-α-O-Ser). The crystal structures of VML in complex with Tn antigen and GalNAc have been determined at the resolution of 1.4 and 1.7 Å, respectively. Unfortunately, most of lectins obtained from natural sources consist in a mixture of forms, which is an undesired feature for biomedical applications. Thus, the recombinant form of VML (rVML) was expressed in Escherichia coli, being obtained soluble and wih high yielding. The crystal structure for rVML, as well as for the complex with Tn antigen, GalNAc and α-Lactose have been determined at resolutions of 1.7, 2.7, 2 and 1.8 Å, respectively, presenting the same overall structure and binding patterns as the wild lectin. Molecular docking analysis of this new structure and other Tn-binding legume lectins to O-mucin fragments differently decorated with this antigen provides a comparative binding profile among these proteins, stressing that subtle alterations that may not influence monosaccharide binding can, nonetheless, directly impact the ability of these lectins to recognize naturally occurring antigens. In addition to the specific biological effects of VML, the structural and binding similarities between it and other lectins commonly used as histological markers (e.g., VVLB4 and SBA) strongly suggest that VML can be used as a new tool for cancer research. This is the first report of crystal structures of a Gal/GalNAc-binding legume lectin from the Dalbergieae tribe. / As lectinas consitem em uma classe diversificada de proteínas, capazes de reconhecer estruturas glicídicas de forma reversível e com alta especificidade, no entanto sem alterar suas estruturas químicas, participando de vários processos celulares importantes. Dentre as diferentes famílias de lectinas, as isoladas a partir de leguminosas são as mais extensivamente estudadas, havendo sido relatada a influências dessas moléculas sobre diversos processos patológicos, incluindo a carcinogênese. Notáveis propriedades antitumorais têm sido detectadas para algumas lectinas de leguminosas, resultantes da sua habilidade em induzir a morte celular ou a autofagia em células cancerígenas, o que atraído atenção para suas possiveis aplicações biomédicas. Além disso, algumas lectinas desse grupo especificas para galactose/N-acetil-D-galactosamina (Gal/GalNAc) têm se mostrado úteis como marcadores histoquímicos na pesquisa do câncer e a caracterização estrutural dessas lectinas em complexo com diferentes epítopos cancerígenos vem sendo realizada com sucesso. A lectina isolada a partir das sementes da leguminosa Vatairea macrocarpa (VML) é uma lectina bem caracterizada específica para Gal/GalNAc capaz de reconhecer especificamente o antígeno Tn (GalNAc-α-O-Ser), naturalmente encontrado em O-mucinas presentes em diferentes tipos de câncer. As estruturas cristalográficas para a VML em complexo com o antígeno Tn e GalNAc foram determinadas com resoluções de 1.4 e 1.7 Å, respectivamente. A maioria das lectinas obtidas a partir de fontes naturais consiste em misturas de diferentes isoformas, uma característica indesejada para aplicações biomédicas. Com base nisso, uma construção recombinante para VML (rVML) foi expressa em Escherichia coli, sendo obtida de forma solúvel em com alto rendimento. A estrutura cristalina para a rVML, bem como para seus complexos com o antígeno Tn, GalNAc e α-Lactose foram determinadas com resoluções de 1.7, 2.7, 2 e 1.8 Å, respectivamente, apresentando a mesma estrutura geral e padrões de interação que a lectina silvestre. Com o intuito de gerar um perfil comparativo entre a VML e outras lectinas de leguminosas capazes de reconhecer o antígeno Tn, foram realizadas análises de docking molecular utilizando fragmentos de O-mucinas diferentemente decorados com o antígeno Tn. Esse perfil ressalta como alterações sutis no elenco ou disposição dos aminoácidos constituintes do sítio de ligação a carboidrato, que talvez não influenciem a capacidade de ligação a monossacarídeo, podem impactar diretamente a habilidade dessas lectinas em reconhecer antígenos em condições naturais. Adicionalmente aos já caracterizados efeitos biológicos relatados para VML, a similaridade entre sua estrutura e perfis de interações quando comparadas a outras lectinas comumente utilizadas como marcadores histoquímicos (e.g., VVLB4 e SBA), sugerem fortemente a possível utilização da VML como uma nova ferramenta na pesquisa do câncer. Esse trabalho consiste no primeiro relato de estruturas cristalográficas para uma lectina de leguminosa específica para Gal/GalNAc da tribo Dalbergieae.

CoordenaÃÃo de AperfeiÃoamento de Pessoal de NÃvel Superior / Objetivou-se avaliar o crescimento da GlirÃdica sepium submetida a diferentes manejos de corte nas condiÃÃes do semiÃrido cearense. O delineamento estatÃstico utilizado foi de blocos ao acaso em parcelas subdivididas. As parcelas constaram de quatro frequÃncias de corte (45, 60, 75 e 90 dias) e trÃs alturas de resÃduo (30, 60 e 90 cm) com quatro repetiÃÃes, sendo que a frequÃncia de corte compreendeu a parcela principal e a altura de resÃduo a subparcela. O experimento foi conduzido no municÃpio de Fortaleza, CearÃ. O plantio foi realizado em marÃo de 2010 em espaÃamento de 1,5 m x 1,0 m com Ãrea experimental de 600 m2 e estande com 336 plantas. O corte de uniformizaÃÃo e aplicaÃÃo dos tratamentos ocorreu em dezembro de 2010, a partir desta data iniciou-se o perÃodo de avaliaÃÃo que foi de 375 dias a quantidade de cortes variou de acordo com os tratamentos, realizando-se 8, 6, 5 e 4 cortes para os tratamentos com as frequÃncias de corte de 45, 60, 75 e 90 dias, respectivamente. A anÃlise das caracterÃsticas de crescimento da gliricÃdia foi obtida atravÃs da Ãrea foliar especÃfica, Ãndice de Ãrea foliar, taxa de crescimento relativo, taxa assimilatÃria lÃquida, taxa de crescimento da cultura, diÃmetro do caule ao nÃvel do solo, diÃmetro dos brotos, nÃmero de brotaÃÃo, nÃmero de folhas por broto, altura da planta, relaÃÃo folha/caule, matÃria seca da planta, da folha e do caule, produÃÃo de massa seca de folha e caule por corte e total e produÃÃo de massa de forragem por corte e total. As plantas manejadas com frequÃncia de corte de 90 dias e altura de resÃduo de 90 cm obtiveram maior Ãrea foliar especÃfica e Ãndice de Ãrea foliar com 48,51 cm2.g-1 e 5,54, respectivamente. A maior taxa de crescimento cultural foi de 2,63 g.m-2.dia-1 obtida no manejo de corte com frequÃncia de 45 dias e altura de resÃduo de 90 cm. No mesmo manejo de corte observou-se o valor mÃximo para taxa de crescimento relativo que foi de 0,016 g.g-1.dia-1 e maior taxa assimilatÃria lÃquida com 1,32 g.m-2.dia-1, ambas as taxas foram obtidas no perÃodo do 3Â ao 4Â corte (perÃodo chuvoso). O diÃmetro do broto para o manejo de corte com frequÃncia de 90 dias combinado com altura de resÃduo de 90 cm foi 0,31 cm superior ao diÃmetro do broto no manejo de corte com frequÃncia de 45 dias e altura de resÃduo de 30 cm, apresentando maior diÃmetro de broto de 0,96 cm para o perÃodo das chuvas e de 0,81 cm para o perÃodo seco, obtendo ainda, maior Ãndice de Ãrea foliar no perÃodo das chuvas e seco. O manejo com frequÃncia de corte de 90 dias combinado com altura de resÃduo de 90 cm obteve maior acÃmulo de massa seca de forragem por corte no perÃodo das chuvas e seco com 5,3 e 4,8 t.ha-1, e total com 10,7 e 9,7 t.ha-1, respectivamente. As plantas submetidas a esse mesmo manejo atingiram a maior produÃÃo de massa verde e seca de forragem por corte, com mÃdia de 18,13 t.ha-1 e 5,09 t.ha-1 em cada corte, respectivamente, produzindo 72,52 t.ha-1 de massa verde de forragem total e 20,36 t.ha-1 de massa seca de forragem total. A frequÃncia de corte e altura de resÃduo influenciam o crescimento e produÃÃo da gliricÃdia. A intensidade de corte 90 cm proporciona melhores taxas de crescimento e quando combinada com a frequÃncia de corte de 90 dias ocorre maior produÃÃo de massa de forragem da gliricÃdia. / The objective was to evaluate the growth of GlirÃdica sepium under different managements cutting conditions semiarid of CearÃ. The statistical design was a randomized block design with split plots. The plots consisted of four cut off frequencies (45, 60, 75 and 90 days) and three heights residue (30, 60 and 90 cm) with four replications, and the cut off frequency comprised the major portion of the residue height of the subplot. The experiment was conducted in the city of Fortaleza, CearÃ. Planting took place in March 2010 at a spacing of 1.5 m x 1.0 m with an area of 600 m2 and experimental stand with 336 plants. Cutting standardization and application of treatments occurred in December 2010, from this date began the evaluation period of 375 days which was the amount of cuts varied with treatments, performing 8, 6, 5 and 4 cuts for treatments with cut off frequencies of 45, 60, 75 and 90 days, respectively. The analysis of the growth characteristics of Gliricidia was obtained by specific leaf area, leaf area index, relative growth rate, net assimilation rate, crop growth rate, stem diameter at ground level, diameter of shoots, number of sprouting, number of leaves, plant height, leaf/stem ratio, plant dry matter, leaf and stem dry mass of leaf and stem by cutting and total production of dry matter per cutting and full. Plants managed with cut off frequency of 90 days and a residue height of 90 cm had higher specific leaf area and leaf area index with 48.51 cm2.g-1 and 5.54, respectively. The highest growth rate cultural was 2.63 g.m-2.dia-1 obtained in the management of cutting frequency of 45 days and residue height of 90 cm. At the same management cut off was observed for the maximum relative growth rate which was 0.016 g.g-1.dia-1 and higher net assimilation rate with 1.32 g.m-2.dia-1, both rates were obtained in period of 3Â to 4Â cut (rainy season). The diameter of the bud for handling cut frequently with 90 days combined residue height of 90 cm was 0.31 cm larger than the diameter of the shoot in the management of cutting frequency of 45 days and residue height of 30 cm, with larger diameter of 0.96 cm shoot for the rainy season and 0.81 cm for the dry period, getting even, greater leaf area index during the rainy season and dry. The handling with cut off frequency of 90 days combined with residue height of 90 cm obtained higher dry matter accumulation by cutting forage during the rainy season and dry with 5.3 and 4.8 t.ha-1, and with full 10.7 t.ha-1 and 9.7, respectively. Plants subjected to the same management reached the highest yield of green mass and dry fodder by cutting, with a mean of 18.13 t.ha-1 and 5.09 t.ha-1 in each cut, respectively, producing 72 52 t.ha-1 fresh weight of total forage and 20.36 t.ha-1 dry weight of total forage. The cut off frequency and height of residue influence the growth and production of Gliricidia. The intensity of cut 90 cm provides better growth rates and when combined with a cut off frequency of 90 days is greater herbage mass production of Gliricidia.

O processo de beneficiamento do zinco, extraído em Vazante pela Companhia Mineira de Metais - CMM produz um rejeito alcalino e com baixa disponibilidade de nutrientes. Esta dissertação tem como objetivo avaliar o potencial de utilização de espécies leguminosas noduladas e micorrizadas na revegetação de barragem de rejeito da CMM. Neste sentido, foram instalados dois experimentos de campo onde foi realizado o plantio prévio de Brachiaria sp. O primeiro experimento foi composto por 36 tratamentos que foram formados por uma combinação de 17 espécies + 1 testemunha (ausência de plantas) na presença e na ausência de esterco de curral (2,0 L) na cova de plantio. Cada unidade experimental foi formada por 20 exemplares da mesma espécie que foram plantadas em covas abertas manualmente (25 x 25 x 25 cm) num espaçamento de 2 x 2 m. Todas as covas receberam a adubação básica formada por 125 g de superfosfato simples e 60 g de cloreto de potássio. Entre as 17 espécies avaliadas, 3 não pertencem a família Leguminosae e receberam, além da adubação básica, cerca de 25 g de sulfato de amônio por cobertura. O segundo experimento foi montado com o objetivo de avaliar o potencial de espécies leguminosas beneficiarem o estabelecimento e crescimento de espécies não leguminosas na revegetação de barragem de rejeito da CMM. Foram utilizadas três espécies leguminosas (Enterolobium scomburkii, Acacia mangium e Acacia holosericea) e três não leguminosas (Lithraea brasiliensis, Cinnamomum glaziovii e Eugenia jambolana) num esquema fatorial (3 x 3) + 1 testemunha, formando dez tratamentos distribuídos em blocos ao acaso com três repetições. Cada parcela foi formada por 20 plantas (10 leguminosas + 10 não leguminosas) plantadas em espaçamento 2 x 2 m e com a mesma adubação básica utilizada no primeiro experimento. Todas as espécies leguminosas utilizadas foram previamente inoculadas com estirpes selecionadas de bactérias fixadoras de Nitrogênio atmosférico e com uma mistura de fungos micorrízicos provenientes da Embrapa/Agrobiologia. Os experimentos foram avaliados quanto ao estabelecimento e crescimento de plantas (altura e diâmetro do colo) aos 4, 12 e 24 meses após o plantio. Os resultados obtidos permitem concluir que dentre as espécies avaliadas, as mais indicadas para a primeira etapa da revegetação da barragem de rejeito da CMM são: Acacia holosericea, Acacia farnesiana, Acacia auriculiformis, Mimosa caesalpiniifolia, Leucaena leucocephala, Mimosa birmucronata, Enterolobium schomburkii e Prosopis juliflora. O sucesso do consórcio de espécies leguminosas e não leguminosas depende da escolha das espécies a serem combinadas, de maneira que não exista uma efetiva competição por água, nutrientes e luz que possa prejudicar as espécies de menor plasticidade. Das combinações avaliadas, as de maiores potencialidades para o programa de revegetação das barragens de rejeito da CMM são aquelas envolvendo a espécieLithraea brasiliensis. / The ore milling process at Vazante by Companhia Mineira de Metais - CMM produce an alkaline and low nutrients content refuse. This work had the aim to evaluate the potential of nodulated and mycorrhized legumes shrubs and trees specie at the revegetation of the CMM´s tailing dam. Therefore, two field experiments were carried out at the tailing dam where Brachiaria sp. was previously seemed. The first experiment was formed by a combination of 17 specie + 1 blank (without plants) in the presence and absence of cattle manure (2.0 L) at planting pit. Each experimental unit was composed by 20 individuals of same specie which were planted in handle holes (25 x 25 x 25 cm) at 2 x 2 m lines. Each planting pit received 125 g of super phosphate and 60 g of potassium chlorate. Among the 17 species evaluated, three are not Leguminosae and also received more 25 g of ammonium sulfate by surface application. The second experiment was carried out with the aim to evaluate the potential of leguminous specie to benefit the establishment and growth of non-leguminous specie at the revegetation of the refuse dam. At this experiment three leguminous specie (Enterolobium schomburkii, Acacia mangium and Acacia holosericea) and three non-leguminous specie (Lithraea brasiliensis, Cinnamomum glaziovii and Eugenia jambolana) were combined in a factorial design (3 x 3) + 1 blank, forming 10 trataments. Each experimental unit was composed by 20 plants (10 legumes + 10 non-legumes) planted and fertilized at the same way used at the first experiment. The leguminous specie were previously inoculated and mycorrhized with efficient strains of N-fixation and a misture of mycorrhizal fungi obtained at Embrapa/Agrobiologia. The experiments were evaluated about the plants establishment and growth (height and steam diameter) at 4, 12 and 24 months after the planting time. The obtained results allowed concluding that the indicated specie, among all evaluated, to the first step of the refuse dam revegetation are: Acacia holosericea, Acacia farnesiana, Acacia auriculiformis, Mimosa caesalpiniifolia, Leucaena leucocephala, Mimosa birmucronata, Enterolobium schomburkii e Prosopis juliflora. The success of the combination of leguminous and non-leguminous specie depends to the choice of the specie to avoid competition by water, nutrients and light which may damage the specie with less plasticity. Among all evaluated combinations the more potentiality one to the CMM´s tailing dam revegetation are which envolving L. brasiliensis.

Made available in DSpace on 2015-04-20T12:22:41Z (GMT). No. of bitstreams: 1 marco.pdf: 749143 bytes, checksum: e1d5d1fa51525a11da8d7d61481e33ed (MD5) Previous issue date: 2012-06-21 / Little one knows on the efficiency of the answer of N2 fixation in legume tree native of the Amazonian when inoculated with specific or homolog rhizobia to demonstrate the use potential of microbial inoculants to stimulate biological technologies in the agricultural production and recovery of soils. The objective of this work was to evaluate the effect of the inoculation with Rhizobia about the growth and biomass distribution in three native legume trees. The selected species were: Inga thibaudiana var. thibaudiana, I. nobilis and Erythrina fusca. Two soils acid of the firm earth they were appraised: yellow oxisol collected under pasture and yellow red ultisol collected in agroforestry systems. The species were sowed in sand and transplanted for bags with 2 soil kg and led in the nursery. In the two soils three forms of supply of N were tested: control treatment (plants not inoculated and without mineral N); plants supplied with 80 kg of N hectare-1; and, plants inoculated with a cocktail of strains of Rhizobia of the INPA collection. Monthly the length of the stem of the plants was evaluated. The crop was made to the 93 days for E. fusca and 165 and 151 days for I. thibaudiana and I. nobilis, being determined the distribution of dry biomass in the leaves, roots, stem and nodules of the plant. The number of nodules was counted and made determinations of leave-N and total-N of the plants. The experimental design was it entirely random, in factorial arrangement 2 x 3 (soils x forms of supply of N), with 9 repetitions. The inoculation with Rhizobia influences the growth, biomass partition, nodulation indicators and absorption of N, having differences in the answer depending on the appraised species. The specificity character or of promiscuity in the selected legume tree is decisive in the answer of the species the inoculation. All the species of legume tree researched adapted better to the soil ultisol compared to the oxisol. It was demonstrated that the employed strains as inoculants in I. thibaudiana var. thibaudiana formed processes of fixation of more efficient N2 than when inoculated in I. nobilis. For E. fusca, considered of behavior non specific as the compatibility with native Rhizobia the practice of the inoculation favored the symbiotic processes in soil oxisol compared to ultisol. It was evidenced that the inoculation technique with Rhizobia has alternative potentiality for supply of nitrogen for the plants, compared the nitrogen fertilization. / Pouco se conhece sobre a eficiência da resposta fixadora de N2 de leguminosas arbóreas nativas da Amazônia quando inoculadas com rizóbios específicos ou homólogos, havendo a necessidade de demonstrar o potencial de uso deste inoculante microbiano para estimular tecnologias biológicas na produção agrícola e recuperação de solos. O objetivo deste trabalho foi avaliar o efeito da inoculação com rizóbios sobre o crescimento e distribuição de biomassa em três leguminosas arbóreas nativas. As espécies selecionadas foram: Inga thibaudiana var. thibaudiana, I. nobilis e Erythrina fusca. Dois solos ácidos da terra firme foram avaliados: Latossolo Amarelo coletado sob pastagem e Argissolo Vermelho Amarelo coletado em sistemas agroflorestais. As espécies foram semeadas em areia e transplantadas para sacos com 2 kg de solo e conduzidas no viveiro. Nos dois solos, três formas de suprimento de N foram testadas: testemunha (plantas não inoculadas e sem N-mineral); plantas supridas com 80 kg de N ha-1; e, plantas inoculadas com um coquetel de estirpes de rizóbios da coleção do INPA. Mensalmente avaliou-se o comprimento do caule das plantas. A colheita foi efetuada aos 93 dias para E. fusca e 165 e 151 dias para I. thibaudiana e I. nobilis, determinando-se a distribuição de biomassa seca nas folhas, raízes, caule e nódulos das plantas. O número de nódulos foi contado e efetuado determinações do N-foliar e N-total das plantas. O delineamento experimental foi o inteiramente casualizado, em arranjo fatorial 2 x 3 (solos x formas de suprimento de N), com 9 repetições. A inoculação com rizóbios influenciou o crescimento, partição de biomassa, indicadores de nodulação e absorção de N, havendo diferenças na resposta dependendo da espécie avaliada. O caráter de especificidade ou de promiscuidade na leguminosa arbórea selecionada é determinante na resposta da espécie a inoculação. Todas as espécies de leguminosas arbóreas pesquisadas adaptaram-se melhor ao solo Argissolo comparado ao Latossolo. Demonstrou-se que as estirpes empregadas como inoculante em I. thibaudiana var. thibaudiana formaram processos de fixação de N2 mais eficientes que quando inoculadas em I. nobilis. Para E fusca, considerada de comportamento não específico quanto a compatibilidade com rizóbios nativos a prática da inoculação favoreceu os processos simbióticos em solo Latossolo comparado ao Argissolo. Evidenciou-se que a técnica de inoculação com rizóbios tem potencialidade alternativa para suprimento de nitrogênio para as plantas, comparado a fertilização nitrogenada.