In recent years, the rhizobial taxonomy changed significantly with the discovery of novel symbiotic associations between legumes and nodulating bacteria. This was aided by the focus shift from studying only agricultural crops to legumes indigenous to certain regions, ultimately to discover new inoculant strains and to uncover the secrets of the rhizobium¬legume symbiosis. In previous studies on the diversity of South African rhizobia, it has become clear that our country has a wealth of rhizobia. Cyclopia is a legume genus, which belongs to the fynbos biome of South Africa. Honeybush tea is a herbal infusion manufactured from the leaves and stems of certain Cyclopia spp. Commercial cultivation of this potentially new agricultural crop is now developed to protect the natural Cyclopia spp. populations from harvesting and ultimately extinction. Superior inoculant strains are necessary for these commercial seedlings. The diversity of root-nodulating strains isolated from 14 Cyclopia spp. was determined using 16S-23S IGS-RFLP and partial 16S rDNA base sequencing. Based on 16S-23S IGS-RFLP and partial 16S rDNA base sequencing most of the isolates, with the exception of seven strains, were found to belong to the genus Burkholderia. More extensive phylogenetic, symbiotic and phenotypic studies of selected strains were performed using near full-length 168 rDNA base sequencing, nodA base sequencing and substrate utilisation analysis. In the genus Burkholderia, the isolates belonged to the novel root-nodulating species Burkholderia tuberum and several novel, undescribed Burkholderia genotypes. However, no new Burkholderia species could formally be proposed, since DNA-DNA hybridisation analysis, which is a prerequisite for the description of new species could not be performed in our laboratory. The seven strains not affiliated with the Burkholderia genus belonged to two Bradyrhizobium genospecies, R tropici and a possibly new genus in the a-Proteobacteria. The nodA sequences of all the Cyclopia isolates corresponded to a large extent, indicating that different chromosomal genotypes harbour the same symbiotic genotype. All the isolates of the Cyclopia genus appear to be acid-tolerant, which is in agreement with the acidic nature of the soil from which the strains were isolated. / Thesis (PhD(Microbiology))--University of Pretoria, 2004. / Microbiology and Plant Pathology / unrestricted

Mycorrhizal associations are very important to plant growth. This is mainly due to the hyphal stands that are able to utilize concentrations of nutrients that would normally be considered unavailable for plant growth, especially phosphorus. These associations are of great importance in soils that have been disturbed by mineral extraction and are consequently low in available nutrients. The ability of mycorrhizal fungi to form associations on disturbed sites may be dictated by a number of factors: moisture, soil pH, soil fertility (including heavy metal ions), salinity, and plant response. Two factorial experiments were conducted with the legume Hedysarum boreale cv utahensis and the mycorrhizal fungus Glomus mosseae. The first to observe the effects of three factors: fungi level (mycorrhizal or non-mycorrhizal), type of phosphate (P) (monocalcium or superphosphate), and irrigation intervals (2, 4, or 8 day) on root and shoot dry weights. The second experiment was conducted to observe the effects of three factors: fungi level, type of P and level of P on root and shoot dry weights, root and shoot P concentration, nodulation and nitrogen fixation. In the first experiment, the combination of fungi level and type of P had a significant effect on root /shoot ratio (weight ). In the second experiment, the interaction of fungi level, type of P and level of P had a significant effect on nodulation. The interaction of P type and P level had a significant effect on the amount of phosphorus found in plant roots and shoots. The factor, fungi level, had a significant effect on shoot dry weight, with mycorrhizal plant's shoots having a significantly greater dry weight than non-mycorrhizal shoots. Mycorrhizal infection may have been greatly reduced by the adverse soil conditions found in the mine spoil (i.e. low pH, high salinity , and high heavy metal concentration). What infection did occur may have been great enough to produce changes in the shoot dry weight, but not great enough to significantly enhance plant P uptake. The reintroduction of mycorrhizae into disturbed areas for reclamation purposes may be important for rapid establishment of plant cover and to speed up succession. Before mycorrhizae can be reestablished in a disturbed area, many potential stress factors need to be addressed and, if possible, overcome.

Pastures in the Intermountain Western United States mainly consist of cool-season grasses which lack production without supplemental nitrogen. Legumes provide nitrogen at reduced cost compared to nitrogen fertilizer. There is a need for proven methods of inter-seeding legumes into existing cool-season grass pastures as well as knowledge of how animals prefer legumes to grasses and how the nutritive value of forages change throughout the growing season. This research provides a resource for effective integration of legumes into pastures of the Intermountain West. Alfalfa, birdsfoot trefoil, and cicer milkvetch were inter-seeded into existing cool-season grass pastures following pretreatments of light tillage, mowing and glyphosate. Early spring and fall inter-seeding was unsuccessful, while late spring was moderately successful with overall alfalfa frequency of 30% after a year. Summer inter-seeding was the most successful with birdsfoot trefoil, alfalfa, and cicer milkvetch frequency of 42, 32, and 22%, respectively. In the animal preference study, when compared to tall fescue, birdsfoot trefoil was always utilized more.In most observations birdsfoot trefoil utilization was higher than orchard grass. While in all comparisons with meadow bromegrass there was no difference in utilization. During early growth, perennial ryegrass utilization was higher than birdsfoot trefoil utilization, but no difference in utilization was detected in later observations. Overall, birdsfoot trefoil was utilized 73% overall while grass utilization was 74, 67, 64, and 53% for perennial ryegrass, meadow bromegrass, orchardgrass and tall fescue, respectively. The interaction of growth stage and time of season on the nutritive value of alfalfa, birdsfoot trefoil, cicer milkvetch, orchardgrass, perennial ryegrass and tall fescue was determined. In the spring to early summer, legume nutritive value decreased rapidly with maturity, in midsummer the nutritive value decreased slowly with maturity, while in late summer the nutritive value remained stable. In the spring, grass nutritive value decreased rapidly, while in mid to late summer all grass regrowth was vegetative and the nutritive value remained stable. By inter-seeding in the summer, effectively managing mixed pastures, and utilizing forage at its highest nutritive value, legumes can benefit the pastures of the Intermountain West.

The legume-rhizobia relationship is an important symbiosis. Studies have found variation in specificity and the functionality of symbiotic specificity can vary among plants of the same species and among rhizobia, as well as in concert with geographical variation. Here, we examined the diversity and geographic structure of rhizobia nodulating Chamaecrista fasciculata, which grows throughout the east-central U.S. and is symbiotic with Bradyrhizobium species. We investigated the association of geography and soil variables on rhizobial diversity by sampling plant nodules and soil across Mississippi and evaluated variation in rhizobia housed in different nodules of individual plants. Using nifH and truA, we conducted phylogenetic analyses and mantel tests but did not find that geography correlates with genetic diversity. However, soil variables and genetic distance were significantly correlated. Lastly, we found that rhizobia across nodules of the same plant varied substantially. These results contribute to the knowledge of rhizobial assemblages in natural populations.

No description available.

No description available.

This study compares the energy costs of synthesizing, distributing, and applying manufactured nitrogen fertilizer to the overall energy costs associated with nitrogen-fixing legume production and use. The energetics of com silage and com grain production under standard and alternative practices are examined. Economic analyses of crop production practices are used to aid the selection of recommended alternative practices. In corn silage production, cover-cropped treatments had a significant advantage over standard practice treatments in terms of overall energy expenditures for field operations. Cover-cropped no-till treatments required an average energy expenditure of 9026 MJ/ha compared to 19,763 MJ/ha required by the standard-practice no-till treatment. Cover-cropped treatments that used disking to kill the cover crops required an average energy expenditure of 9781 MJ/ha compared to 18,488 MJ /ha required by the standard-practice winter-fallow treatment. Alternative-practice treatments that utilized vetches to provide nitrogen for com production performed significantly better than standard-practice treatments in terms of energy use per unit of crop output. In addition, the alternative hairy vetch - no-till treatment produced a $33/ha greater average net revenue than the standard-practice no-till treatment. Weed control energy requirements for cover-cropped ridge-tillage com grain production were compared. Broadcast application of pre-emergence herbicides required an energy expenditure of 1160 MJ fha. Cultivation of ridges to control weeds consumed 380 MJ/ha. Economic costs of ridge cultivation were $14/ha. Broadcast application of pre-emergence herbicides cost $49/ha. / Master of Science

The lack of consistent summer pasture supply is a major limitation to livestock production in the mid-Atlantic region. Perennial warm-season grasses might provide a solution if managed for high quality. Experiments were conducted on separate well-established stands of Caucasian bluestem and 'Cave-in-Rock' switchgrass at the Kentland Farm near Blacksburg, VA. Stages of grass maturity at harvest simulating pasture and hay systems were tested. Six interseeded legume species and two grass monoculture checks, one with 56 kg N/ha applied in spring and after each harvest, the other with no N, were imposed as sub-plots. Legume species included alfalfa, red clover, sericea lespedeza, annual lespedeza, Illinois bundleflower, and purple prairieclover. Inter-seeded legumes contributed a significant amount to yield and quality of perennial warm-season grasses in the legume-establishment year. In the year after establishment, grass mixtures with alfalfa, red clover, and, for switchgrass, sericea lespedeza yielded as much forage as N-fertilized grasses. Alfalfa and red clover altered the distribution of yield of the grasses, and may not be as compatible with perennial warm-season grasses as sericea lespedeza in the long-term. Interseeded legumes improved quality considerably in the second year. / Master of Science

Matua prairie grass has a potential to extend the grazing season in Virginia due to its higher early spring and fall production. However, little is known about the compatibility of Matua prairie grass with legumes or the effects of legumes on the yield and quality of Matua prairie grass/legume mixtures. An experiment was conducted in 1998 and 1999 to investigate the botanical composition, yield, and chemical composition of Matua prairie grass grown with legumes. Legume treatments consisting of ladino clover (Trifolium repens), red clover (Trifolium pratense), alfalfa (Medicago sativa), and annual lespedeza (Lespedeza stipulacea) were drilled into a Matua prairie grass stand. Nitrogen was applied once each fall at two treatment levels of 0 or 84 kg/ha. The experiment was arranged in a randomized split block design with four replications. Legume treatments had no effect on percentage Matua prairie grass or total dry matter yield in 1998. However, in 1999 the ladino clover and red clover treatments increased (P<0.05) total dry matter yield, but also resulted in a substantial decrease (P<0.05) in percentage Matua prairie grass. Nitrogen application in the fall of 1998 had a residual effect (P<0.05) on the percentage Matua prairie grass and yield in 1999. The highest response to nitrogen fertilization occurred in the harvest immediately after fertilization, in October of 1999, which resulted in the largest increase (P<0.05) in percentage Matua prairie grass and yield, and the greatest decrease (P<0.05) in percentage legumes. The legume and nitrogen treatments similarly influenced the chemical composition of the Matua prairie grass/legume mixed forage. Ladino clover, red clover, and alfalfa treatments generally improved forage quality as indicated by a decrease (P<0.05) in NDF, ADF, hemicellulose, and cellulose, and an increase (P<0.05) in CP and IVDMD. Nitrogen fertilization did not influence the chemical composition of the forages to the same extent as the legume treatments, as a decrease in fiber components and an increase in CP and IVDMD were observed due to nitrogen. Overall, alfalfa appeared to be most compatible with Matua prairie grass, and the incorporation of alfalfa into a Matua prairie grass stand resulted in some improvements in total dry matter yield and nutritive value of the forage, without the detrimental suppression of Matua prairie grass. / Master of Science

Bakgrund: Generellt sett är den växtbaserade kosten den bästa för miljön, speciellt om man tillagar mat av exempelvis baljväxter ifrån grunden istället för att använda sig utav substitut. Intresset av vegetarisk mat har ökat och om man äter en vegetarisk kost eller byter ut köttet till baljväxter kan man minska risken för eventuella sjukdomar. Vi har idag 13 olika sorters baljväxter som odlas, skördas, förpackas och kontrolleras här i Sverige. Syfte: Syftet med denna studie var att undersöka hur vuxna människor i Sverige involverar baljväxter i kosten, hur nöjda de är med sin konsumtion och vilka motiv som ligger bakom konsumtionen. Metod: Den metod som valdes var en kvantitativ metod där vuxna människor mellan åldrarna 25–65 år fick svara på en webbenkät. Detta eftersom vi ville samla in en större mängd data för att jämföra resultat från våra informanter baserat på ålder och kön. Resultat: Motiven till att konsumera baljväxter var att för att det var gott, följt av att de är hälsosamma. Varför de inte konsumerade baljväxter handlade om ovana, okunskap, sensorik, fysiskt obehag (magont). Slutsats: För att svara till syftet om hur informanterna involverade baljväxter i kosten så var det främst vid middag och lunch som baljväxter konsumerades. Och de vanligaste baljväxterna som konsumerades var gröna ärtor och haricots verts.Mer än hälften av våra informanter ville konsumera mer baljväxter men flera av dem var även med sin konsumtion. Vad som avgjorde våra informanters inköp av baljväxter var priset och svensk producerat. Motiven till att de konsumerade baljväxter var att de tyckte det var gott samt att de tyckte de vart hälsosamma. / Background: Generally speaking, the plant-based diet is the best for the environment, especially if you prepare food from, for example, legumes from scratch instead of using substitutes. Interest in vegetarian food has increased and if you eat a vegetarian diet or replace meat with legumes, you can reduce the risk of possible diseases. We currently have 13 different types of legumes that are grown, harvested, packaged and controlled here in Sweden. Aim: The purpose of this study was to investigate how adults in Sweden include legumes in their diet, how satisfied they are with their consumption and what motives lie behind the consumption. Method: The method chosen was a quantitative method where adults between the ages of 25-65 years answered a web survey. This is because we wanted to collect a larger amount of data to compare results from our informants based on age and gender. Results: The motives for consuming legumes were because it was tasty, followed by the fact that they are healthy. Why they did not consume legumes was about unfamiliarity, ignorance, sensory, physical discomfort (stomach). Conclusion: To answer the purpose of how the informant's involved legumes in the diet, it was mainly at dinner and lunch that legumes were consumed. And the most common legumes consumed were green peas and green beans. More than half of our informants wanted to consume more legumes, but several of them were also with their consumption. What determined our informants' purchase of legumes was the price and Swedish production. The reasons why they consumed legumes were that they thought it was good and that they thought it was healthy.